Amino acid dipepetide frequency for Pseudomonas phage MR299-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.864AlaAla: 8.864 ± 1.518
1.09AlaCys: 1.09 ± 0.339
4.505AlaAsp: 4.505 ± 0.461
5.376AlaGlu: 5.376 ± 0.911
2.688AlaPhe: 2.688 ± 0.417
6.539AlaGly: 6.539 ± 1.162
1.162AlaHis: 1.162 ± 0.259
4.65AlaIle: 4.65 ± 0.507
4.577AlaLys: 4.577 ± 0.622
7.992AlaLeu: 7.992 ± 0.917
2.398AlaMet: 2.398 ± 0.416
3.487AlaAsn: 3.487 ± 0.453
2.688AlaPro: 2.688 ± 0.596
4.287AlaGln: 4.287 ± 0.843
3.415AlaArg: 3.415 ± 0.52
5.158AlaSer: 5.158 ± 0.733
3.923AlaThr: 3.923 ± 0.603
6.103AlaVal: 6.103 ± 0.812
0.944AlaTrp: 0.944 ± 0.31
2.979AlaTyr: 2.979 ± 0.524
0.0AlaXaa: 0.0 ± 0.0
Cys
0.799CysAla: 0.799 ± 0.203
0.145CysCys: 0.145 ± 0.093
0.436CysAsp: 0.436 ± 0.167
0.727CysGlu: 0.727 ± 0.19
0.145CysPhe: 0.145 ± 0.112
1.235CysGly: 1.235 ± 0.32
0.509CysHis: 0.509 ± 0.16
0.509CysIle: 0.509 ± 0.205
0.291CysLys: 0.291 ± 0.142
0.654CysLeu: 0.654 ± 0.202
0.145CysMet: 0.145 ± 0.119
0.509CysAsn: 0.509 ± 0.175
0.581CysPro: 0.581 ± 0.212
0.363CysGln: 0.363 ± 0.153
0.872CysArg: 0.872 ± 0.23
0.436CysSer: 0.436 ± 0.175
0.363CysThr: 0.363 ± 0.16
0.727CysVal: 0.727 ± 0.272
0.073CysTrp: 0.073 ± 0.07
0.727CysTyr: 0.727 ± 0.24
0.0CysXaa: 0.0 ± 0.0
Asp
5.231AspAla: 5.231 ± 0.542
0.509AspCys: 0.509 ± 0.21
3.851AspAsp: 3.851 ± 0.512
4.722AspGlu: 4.722 ± 0.929
2.398AspPhe: 2.398 ± 0.42
4.505AspGly: 4.505 ± 0.603
1.744AspHis: 1.744 ± 0.441
4.214AspIle: 4.214 ± 0.468
2.688AspLys: 2.688 ± 0.558
6.03AspLeu: 6.03 ± 0.576
1.889AspMet: 1.889 ± 0.34
2.107AspAsn: 2.107 ± 0.447
3.705AspPro: 3.705 ± 0.556
1.526AspGln: 1.526 ± 0.343
3.633AspArg: 3.633 ± 0.501
2.979AspSer: 2.979 ± 0.474
3.269AspThr: 3.269 ± 0.44
2.833AspVal: 2.833 ± 0.415
1.962AspTrp: 1.962 ± 0.377
1.889AspTyr: 1.889 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
6.393GluAla: 6.393 ± 0.988
0.581GluCys: 0.581 ± 0.19
5.376GluAsp: 5.376 ± 0.686
6.539GluGlu: 6.539 ± 0.973
2.107GluPhe: 2.107 ± 0.333
6.176GluGly: 6.176 ± 0.629
1.308GluHis: 1.308 ± 0.276
2.688GluIle: 2.688 ± 0.383
3.269GluLys: 3.269 ± 0.607
6.176GluLeu: 6.176 ± 0.718
2.398GluMet: 2.398 ± 0.408
2.688GluAsn: 2.688 ± 0.359
1.744GluPro: 1.744 ± 0.344
2.543GluGln: 2.543 ± 0.449
5.231GluArg: 5.231 ± 0.694
2.979GluSer: 2.979 ± 0.644
3.415GluThr: 3.415 ± 0.596
6.975GluVal: 6.975 ± 0.871
0.799GluTrp: 0.799 ± 0.218
1.889GluTyr: 1.889 ± 0.41
0.0GluXaa: 0.0 ± 0.0
Phe
2.398PheAla: 2.398 ± 0.425
0.436PheCys: 0.436 ± 0.15
2.325PheAsp: 2.325 ± 0.521
1.889PheGlu: 1.889 ± 0.42
1.816PhePhe: 1.816 ± 0.412
4.141PheGly: 4.141 ± 0.537
0.799PheHis: 0.799 ± 0.247
2.47PheIle: 2.47 ± 0.45
2.034PheLys: 2.034 ± 0.392
3.705PheLeu: 3.705 ± 0.42
0.944PheMet: 0.944 ± 0.214
1.38PheAsn: 1.38 ± 0.361
1.308PhePro: 1.308 ± 0.334
1.526PheGln: 1.526 ± 0.386
1.598PheArg: 1.598 ± 0.397
2.616PheSer: 2.616 ± 0.399
2.325PheThr: 2.325 ± 0.318
3.269PheVal: 3.269 ± 0.521
0.872PheTrp: 0.872 ± 0.237
1.38PheTyr: 1.38 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
7.193GlyAla: 7.193 ± 1.192
0.872GlyCys: 0.872 ± 0.25
5.013GlyAsp: 5.013 ± 0.649
4.505GlyGlu: 4.505 ± 0.68
2.979GlyPhe: 2.979 ± 0.557
7.12GlyGly: 7.12 ± 1.094
2.325GlyHis: 2.325 ± 0.425
4.722GlyIle: 4.722 ± 0.521
4.722GlyLys: 4.722 ± 0.652
6.248GlyLeu: 6.248 ± 0.734
2.543GlyMet: 2.543 ± 0.475
4.432GlyAsn: 4.432 ± 0.607
2.833GlyPro: 2.833 ± 0.474
3.197GlyGln: 3.197 ± 0.453
4.214GlyArg: 4.214 ± 0.57
6.466GlySer: 6.466 ± 0.845
4.287GlyThr: 4.287 ± 0.621
5.74GlyVal: 5.74 ± 0.625
1.09GlyTrp: 1.09 ± 0.31
2.18GlyTyr: 2.18 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
1.308HisAla: 1.308 ± 0.337
0.291HisCys: 0.291 ± 0.15
0.727HisAsp: 0.727 ± 0.271
1.744HisGlu: 1.744 ± 0.381
0.944HisPhe: 0.944 ± 0.282
1.453HisGly: 1.453 ± 0.295
0.509HisHis: 0.509 ± 0.232
1.308HisIle: 1.308 ± 0.324
1.162HisLys: 1.162 ± 0.264
2.543HisLeu: 2.543 ± 0.478
0.509HisMet: 0.509 ± 0.164
0.727HisAsn: 0.727 ± 0.216
0.799HisPro: 0.799 ± 0.224
0.654HisGln: 0.654 ± 0.2
1.38HisArg: 1.38 ± 0.309
0.799HisSer: 0.799 ± 0.211
1.09HisThr: 1.09 ± 0.296
1.017HisVal: 1.017 ± 0.274
0.654HisTrp: 0.654 ± 0.31
0.654HisTyr: 0.654 ± 0.266
0.0HisXaa: 0.0 ± 0.0
Ile
3.996IleAla: 3.996 ± 0.53
0.363IleCys: 0.363 ± 0.146
2.543IleAsp: 2.543 ± 0.444
2.979IleGlu: 2.979 ± 0.547
2.543IlePhe: 2.543 ± 0.494
4.94IleGly: 4.94 ± 0.531
1.09IleHis: 1.09 ± 0.278
2.906IleIle: 2.906 ± 0.471
3.487IleLys: 3.487 ± 0.517
3.923IleLeu: 3.923 ± 0.516
0.944IleMet: 0.944 ± 0.215
1.889IleAsn: 1.889 ± 0.372
3.56IlePro: 3.56 ± 0.615
2.47IleGln: 2.47 ± 0.483
3.633IleArg: 3.633 ± 0.59
2.688IleSer: 2.688 ± 0.48
2.688IleThr: 2.688 ± 0.461
3.415IleVal: 3.415 ± 0.606
0.509IleTrp: 0.509 ± 0.172
2.18IleTyr: 2.18 ± 0.387
0.0IleXaa: 0.0 ± 0.0
Lys
4.94LysAla: 4.94 ± 0.808
0.436LysCys: 0.436 ± 0.181
4.359LysAsp: 4.359 ± 0.531
3.996LysGlu: 3.996 ± 0.462
2.252LysPhe: 2.252 ± 0.382
3.342LysGly: 3.342 ± 0.542
1.38LysHis: 1.38 ± 0.303
2.18LysIle: 2.18 ± 0.364
3.269LysLys: 3.269 ± 0.537
4.359LysLeu: 4.359 ± 0.607
1.671LysMet: 1.671 ± 0.333
2.034LysAsn: 2.034 ± 0.331
2.47LysPro: 2.47 ± 0.494
1.962LysGln: 1.962 ± 0.328
3.197LysArg: 3.197 ± 0.661
3.197LysSer: 3.197 ± 0.418
2.688LysThr: 2.688 ± 0.398
4.141LysVal: 4.141 ± 0.702
1.235LysTrp: 1.235 ± 0.306
2.398LysTyr: 2.398 ± 0.395
0.0LysXaa: 0.0 ± 0.0
Leu
6.611LeuAla: 6.611 ± 0.656
0.799LeuCys: 0.799 ± 0.215
5.231LeuAsp: 5.231 ± 0.587
6.248LeuGlu: 6.248 ± 0.809
3.342LeuPhe: 3.342 ± 0.513
6.466LeuGly: 6.466 ± 0.86
1.017LeuHis: 1.017 ± 0.268
3.778LeuIle: 3.778 ± 0.544
5.667LeuLys: 5.667 ± 0.609
6.466LeuLeu: 6.466 ± 0.644
3.487LeuMet: 3.487 ± 0.662
4.141LeuAsn: 4.141 ± 0.464
3.778LeuPro: 3.778 ± 0.561
3.633LeuGln: 3.633 ± 0.566
5.958LeuArg: 5.958 ± 0.606
4.577LeuSer: 4.577 ± 0.613
3.996LeuThr: 3.996 ± 0.54
5.231LeuVal: 5.231 ± 0.582
0.654LeuTrp: 0.654 ± 0.221
2.398LeuTyr: 2.398 ± 0.495
0.0LeuXaa: 0.0 ± 0.0
Met
3.051MetAla: 3.051 ± 0.574
0.218MetCys: 0.218 ± 0.126
1.598MetAsp: 1.598 ± 0.284
1.816MetGlu: 1.816 ± 0.418
0.872MetPhe: 0.872 ± 0.268
2.252MetGly: 2.252 ± 0.658
0.363MetHis: 0.363 ± 0.153
1.526MetIle: 1.526 ± 0.312
2.252MetLys: 2.252 ± 0.354
2.107MetLeu: 2.107 ± 0.393
0.509MetMet: 0.509 ± 0.198
1.162MetAsn: 1.162 ± 0.411
1.162MetPro: 1.162 ± 0.239
0.872MetGln: 0.872 ± 0.217
1.38MetArg: 1.38 ± 0.275
2.325MetSer: 2.325 ± 0.427
1.962MetThr: 1.962 ± 0.42
1.744MetVal: 1.744 ± 0.388
0.509MetTrp: 0.509 ± 0.192
0.581MetTyr: 0.581 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
2.979AsnAla: 2.979 ± 0.458
0.581AsnCys: 0.581 ± 0.234
2.18AsnAsp: 2.18 ± 0.331
3.197AsnGlu: 3.197 ± 0.513
2.034AsnPhe: 2.034 ± 0.448
4.141AsnGly: 4.141 ± 0.672
1.162AsnHis: 1.162 ± 0.255
3.124AsnIle: 3.124 ± 0.497
1.526AsnLys: 1.526 ± 0.308
3.487AsnLeu: 3.487 ± 0.486
0.727AsnMet: 0.727 ± 0.23
1.816AsnAsn: 1.816 ± 0.432
2.325AsnPro: 2.325 ± 0.485
1.816AsnGln: 1.816 ± 0.382
2.906AsnArg: 2.906 ± 0.358
1.671AsnSer: 1.671 ± 0.349
2.616AsnThr: 2.616 ± 0.47
2.906AsnVal: 2.906 ± 0.428
0.944AsnTrp: 0.944 ± 0.248
1.453AsnTyr: 1.453 ± 0.301
0.0AsnXaa: 0.0 ± 0.0
Pro
2.761ProAla: 2.761 ± 0.387
0.363ProCys: 0.363 ± 0.144
3.197ProAsp: 3.197 ± 0.479
4.505ProGlu: 4.505 ± 0.648
2.398ProPhe: 2.398 ± 0.347
2.979ProGly: 2.979 ± 0.479
0.872ProHis: 0.872 ± 0.245
1.744ProIle: 1.744 ± 0.371
2.979ProLys: 2.979 ± 0.434
2.688ProLeu: 2.688 ± 0.373
1.017ProMet: 1.017 ± 0.282
1.962ProAsn: 1.962 ± 0.448
1.526ProPro: 1.526 ± 0.531
2.034ProGln: 2.034 ± 0.411
1.744ProArg: 1.744 ± 0.388
2.252ProSer: 2.252 ± 0.416
2.107ProThr: 2.107 ± 0.435
2.833ProVal: 2.833 ± 0.487
0.727ProTrp: 0.727 ± 0.191
1.598ProTyr: 1.598 ± 0.392
0.0ProXaa: 0.0 ± 0.0
Gln
5.086GlnAla: 5.086 ± 0.639
0.218GlnCys: 0.218 ± 0.107
2.398GlnAsp: 2.398 ± 0.386
3.705GlnGlu: 3.705 ± 0.518
1.017GlnPhe: 1.017 ± 0.276
3.124GlnGly: 3.124 ± 0.649
0.363GlnHis: 0.363 ± 0.191
1.38GlnIle: 1.38 ± 0.266
1.816GlnLys: 1.816 ± 0.372
3.342GlnLeu: 3.342 ± 0.519
1.308GlnMet: 1.308 ± 0.397
1.816GlnAsn: 1.816 ± 0.456
0.944GlnPro: 0.944 ± 0.251
1.526GlnGln: 1.526 ± 0.4
2.906GlnArg: 2.906 ± 0.526
1.744GlnSer: 1.744 ± 0.287
1.671GlnThr: 1.671 ± 0.387
2.543GlnVal: 2.543 ± 0.34
0.654GlnTrp: 0.654 ± 0.221
1.235GlnTyr: 1.235 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
4.577ArgAla: 4.577 ± 0.762
0.436ArgCys: 0.436 ± 0.176
3.269ArgAsp: 3.269 ± 0.514
2.979ArgGlu: 2.979 ± 0.511
2.252ArgPhe: 2.252 ± 0.354
4.505ArgGly: 4.505 ± 0.526
1.09ArgHis: 1.09 ± 0.314
3.851ArgIle: 3.851 ± 0.443
3.633ArgLys: 3.633 ± 0.622
5.449ArgLeu: 5.449 ± 0.746
1.889ArgMet: 1.889 ± 0.417
3.342ArgAsn: 3.342 ± 0.545
2.47ArgPro: 2.47 ± 0.455
2.688ArgGln: 2.688 ± 0.412
3.342ArgArg: 3.342 ± 0.523
3.124ArgSer: 3.124 ± 0.55
2.398ArgThr: 2.398 ± 0.423
4.65ArgVal: 4.65 ± 0.691
1.09ArgTrp: 1.09 ± 0.285
1.453ArgTyr: 1.453 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
4.359SerAla: 4.359 ± 0.755
0.799SerCys: 0.799 ± 0.269
3.415SerAsp: 3.415 ± 0.388
4.577SerGlu: 4.577 ± 0.694
2.034SerPhe: 2.034 ± 0.475
5.594SerGly: 5.594 ± 0.54
1.308SerHis: 1.308 ± 0.276
3.778SerIle: 3.778 ± 0.45
2.906SerLys: 2.906 ± 0.537
3.923SerLeu: 3.923 ± 0.582
1.671SerMet: 1.671 ± 0.372
2.47SerAsn: 2.47 ± 0.398
2.398SerPro: 2.398 ± 0.456
2.107SerGln: 2.107 ± 0.379
2.761SerArg: 2.761 ± 0.471
3.996SerSer: 3.996 ± 0.582
2.252SerThr: 2.252 ± 0.473
3.851SerVal: 3.851 ± 0.575
1.162SerTrp: 1.162 ± 0.292
1.816SerTyr: 1.816 ± 0.415
0.0SerXaa: 0.0 ± 0.0
Thr
4.505ThrAla: 4.505 ± 0.678
0.363ThrCys: 0.363 ± 0.156
2.325ThrAsp: 2.325 ± 0.426
3.269ThrGlu: 3.269 ± 0.506
2.47ThrPhe: 2.47 ± 0.353
4.069ThrGly: 4.069 ± 0.563
0.799ThrHis: 0.799 ± 0.224
2.47ThrIle: 2.47 ± 0.443
2.325ThrLys: 2.325 ± 0.43
5.376ThrLeu: 5.376 ± 0.596
1.38ThrMet: 1.38 ± 0.238
2.034ThrAsn: 2.034 ± 0.345
3.197ThrPro: 3.197 ± 0.526
2.18ThrGln: 2.18 ± 0.43
2.979ThrArg: 2.979 ± 0.404
3.124ThrSer: 3.124 ± 0.476
2.833ThrThr: 2.833 ± 0.495
3.633ThrVal: 3.633 ± 0.515
0.872ThrTrp: 0.872 ± 0.322
1.453ThrTyr: 1.453 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
5.231ValAla: 5.231 ± 0.658
1.017ValCys: 1.017 ± 0.295
4.65ValAsp: 4.65 ± 0.601
5.013ValGlu: 5.013 ± 0.619
2.979ValPhe: 2.979 ± 0.446
5.958ValGly: 5.958 ± 0.642
1.744ValHis: 1.744 ± 0.352
2.906ValIle: 2.906 ± 0.501
3.705ValLys: 3.705 ± 0.442
5.086ValLeu: 5.086 ± 0.64
1.671ValMet: 1.671 ± 0.363
3.487ValAsn: 3.487 ± 0.525
2.107ValPro: 2.107 ± 0.374
1.962ValGln: 1.962 ± 0.321
3.851ValArg: 3.851 ± 0.446
4.432ValSer: 4.432 ± 0.561
4.65ValThr: 4.65 ± 0.496
5.522ValVal: 5.522 ± 0.734
1.017ValTrp: 1.017 ± 0.298
2.979ValTyr: 2.979 ± 0.608
0.0ValXaa: 0.0 ± 0.0
Trp
1.235TrpAla: 1.235 ± 0.271
0.218TrpCys: 0.218 ± 0.104
1.453TrpAsp: 1.453 ± 0.28
1.308TrpGlu: 1.308 ± 0.281
0.654TrpPhe: 0.654 ± 0.24
1.526TrpGly: 1.526 ± 0.297
0.0TrpHis: 0.0 ± 0.0
0.727TrpIle: 0.727 ± 0.238
1.598TrpLys: 1.598 ± 0.367
1.235TrpLeu: 1.235 ± 0.258
0.509TrpMet: 0.509 ± 0.173
0.654TrpAsn: 0.654 ± 0.207
0.654TrpPro: 0.654 ± 0.243
0.363TrpGln: 0.363 ± 0.153
1.308TrpArg: 1.308 ± 0.29
0.799TrpSer: 0.799 ± 0.33
0.944TrpThr: 0.944 ± 0.248
0.509TrpVal: 0.509 ± 0.214
0.291TrpTrp: 0.291 ± 0.112
0.581TrpTyr: 0.581 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.308TyrAla: 1.308 ± 0.312
0.509TyrCys: 0.509 ± 0.178
2.833TyrAsp: 2.833 ± 0.433
2.18TyrGlu: 2.18 ± 0.483
1.308TyrPhe: 1.308 ± 0.246
2.616TyrGly: 2.616 ± 0.365
0.727TyrHis: 0.727 ± 0.208
1.816TyrIle: 1.816 ± 0.344
1.598TyrLys: 1.598 ± 0.33
2.688TyrLeu: 2.688 ± 0.493
0.581TyrMet: 0.581 ± 0.24
1.526TyrAsn: 1.526 ± 0.345
2.034TyrPro: 2.034 ± 0.39
1.09TyrGln: 1.09 ± 0.241
2.18TyrArg: 2.18 ± 0.283
1.816TyrSer: 1.816 ± 0.396
2.18TyrThr: 2.18 ± 0.346
2.325TyrVal: 2.325 ± 0.481
0.509TyrTrp: 0.509 ± 0.238
1.453TyrTyr: 1.453 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski