Yellowstone lake phycodnavirus 2

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0YMN5|A0A0P0YMN5_9PHYC Putative 2OG-Fe(II) oxygenase OS=Yellowstone lake phycodnavirus 2 OX=1586714 PE=4 SV=1
MM1 pKa = 7.9GINDD5 pKa = 3.93EE6 pKa = 4.37YY7 pKa = 11.74SRR9 pKa = 11.84DD10 pKa = 3.47AYY12 pKa = 11.03NYY14 pKa = 7.37EE15 pKa = 3.81QAYY18 pKa = 10.76DD19 pKa = 3.65SDD21 pKa = 4.99DD22 pKa = 4.2SVDD25 pKa = 3.86SPDD28 pKa = 5.03PIHH31 pKa = 7.37PEE33 pKa = 3.99DD34 pKa = 3.47WQDD37 pKa = 2.94WYY39 pKa = 11.59SEE41 pKa = 4.04QLLDD45 pKa = 2.84AWMTIRR51 pKa = 11.84QLAEE55 pKa = 3.38EE56 pKa = 5.83DD57 pKa = 3.78YY58 pKa = 8.77TQVRR62 pKa = 11.84VGFPQFVEE70 pKa = 5.34FVMNPGDD77 pKa = 4.46LYY79 pKa = 10.95TEE81 pKa = 4.03NAMSQRR87 pKa = 11.84VVAMWNGIKK96 pKa = 10.19DD97 pKa = 3.37IQVIRR102 pKa = 11.84EE103 pKa = 3.95NLTVEE108 pKa = 5.32NFAAWANINIGG119 pKa = 3.39

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0YMZ0|A0A0P0YMZ0_9PHYC Uncharacterized protein OS=Yellowstone lake phycodnavirus 2 OX=1586714 PE=3 SV=1
MM1 pKa = 7.39ILFRR5 pKa = 11.84ISRR8 pKa = 11.84HH9 pKa = 5.79KK10 pKa = 10.72SLQNLFRR17 pKa = 11.84WRR19 pKa = 11.84IQKK22 pKa = 7.21MTKK25 pKa = 9.98KK26 pKa = 10.41INFLTKK32 pKa = 10.02SSTRR36 pKa = 11.84SSFRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84SMNPRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84IFFPAVV55 pKa = 2.84

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

225

0

225

53499

49

1556

237.8

26.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.028 ± 0.252

1.54 ± 0.112

4.849 ± 0.164

5.282 ± 0.256

4.615 ± 0.134

6.609 ± 0.309

2.054 ± 0.108

5.553 ± 0.12

6.34 ± 0.357

8.355 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.108

5.428 ± 0.225

4.871 ± 0.165

3.529 ± 0.115

5.219 ± 0.23

6.69 ± 0.289

6.783 ± 0.335

7.228 ± 0.153

1.557 ± 0.075

3.738 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski