Nocardioides baekrokdamisoli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2935 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G9J1S0|A0A3G9J1S0_9ACTN Inositol-3-phosphate synthase OS=Nocardioides baekrokdamisoli OX=1804624 GN=ino1 PE=3 SV=1
MM1 pKa = 7.57SYY3 pKa = 11.33DD4 pKa = 3.35LAVWEE9 pKa = 4.8GEE11 pKa = 4.21QPISDD16 pKa = 3.87AAALDD21 pKa = 4.52CYY23 pKa = 10.45LKK25 pKa = 11.19LMDD28 pKa = 4.3RR29 pKa = 11.84MEE31 pKa = 5.29SGDD34 pKa = 4.24HH35 pKa = 6.46EE36 pKa = 4.5PTPTPAIMAFVEE48 pKa = 4.28ALTARR53 pKa = 11.84WPDD56 pKa = 3.2TDD58 pKa = 4.22EE59 pKa = 5.26AWDD62 pKa = 4.27DD63 pKa = 5.7DD64 pKa = 4.06EE65 pKa = 5.59TPWADD70 pKa = 4.11APIINNAFGDD80 pKa = 4.52AIYY83 pKa = 10.04FAMTYY88 pKa = 10.14SGAEE92 pKa = 3.64RR93 pKa = 11.84AVPFAAQCASEE104 pKa = 4.45LGLVCFDD111 pKa = 4.25PQAEE115 pKa = 4.42SLVSARR121 pKa = 4.5

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G9J3E8|A0A3G9J3E8_9ACTN Uncharacterized protein OS=Nocardioides baekrokdamisoli OX=1804624 GN=Back2_22410 PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.41GRR40 pKa = 11.84NKK42 pKa = 10.37LAVV45 pKa = 3.36

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2935

0

2935

913977

39

2937

311.4

33.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.985 ± 0.061

0.74 ± 0.013

5.893 ± 0.04

5.266 ± 0.051

2.992 ± 0.025

8.927 ± 0.041

2.246 ± 0.022

4.534 ± 0.028

2.542 ± 0.035

9.792 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.009 ± 0.018

2.334 ± 0.025

5.275 ± 0.035

2.921 ± 0.025

6.769 ± 0.047

5.741 ± 0.033

6.38 ± 0.048

8.852 ± 0.04

1.579 ± 0.023

2.221 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski