Amino acid dipepetide frequency for Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.008AlaAla: 2.008 ± 0.088	0.972AlaCys: 0.972 ± 0.045	2.662AlaAsp: 2.662 ± 0.072	3.924AlaGlu: 3.924 ± 0.094	3.544AlaPhe: 3.544 ± 0.092	4.684AlaGly: 4.684 ± 0.111	1.282AlaHis: 1.282 ± 0.047	4.918AlaIle: 4.918 ± 0.1	5.053AlaLys: 5.053 ± 0.114	7.915AlaLeu: 7.915 ± 0.128	1.56AlaMet: 1.56 ± 0.055	1.862AlaAsn: 1.862 ± 0.068	1.904AlaPro: 1.904 ± 0.059	2.406AlaGln: 2.406 ± 0.069	2.872AlaArg: 2.872 ± 0.071	3.665AlaSer: 3.665 ± 0.088	2.489AlaThr: 2.489 ± 0.069	5.359AlaVal: 5.359 ± 0.101	0.56AlaTrp: 0.56 ± 0.032	3.18AlaTyr: 3.18 ± 0.085	0.0AlaXaa: 0.0 ± 0.0
Cys	0.729CysAla: 0.729 ± 0.038	0.135CysCys: 0.135 ± 0.017	0.445CysAsp: 0.445 ± 0.033	0.616CysGlu: 0.616 ± 0.03	0.362CysPhe: 0.362 ± 0.028	1.008CysGly: 1.008 ± 0.05	0.254CysHis: 0.254 ± 0.022	0.702CysIle: 0.702 ± 0.04	0.623CysLys: 0.623 ± 0.036	0.872CysLeu: 0.872 ± 0.047	0.256CysMet: 0.256 ± 0.022	0.294CysAsn: 0.294 ± 0.027	0.661CysPro: 0.661 ± 0.039	0.312CysGln: 0.312 ± 0.025	0.438CysArg: 0.438 ± 0.029	0.601CysSer: 0.601 ± 0.038	0.484CysThr: 0.484 ± 0.034	0.719CysVal: 0.719 ± 0.041	0.079CysTrp: 0.079 ± 0.013	0.387CysTyr: 0.387 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	3.261AspAla: 3.261 ± 0.076	0.393AspCys: 0.393 ± 0.03	1.621AspAsp: 1.621 ± 0.054	3.364AspGlu: 3.364 ± 0.086	2.635AspPhe: 2.635 ± 0.072	3.141AspGly: 3.141 ± 0.073	0.74AspHis: 0.74 ± 0.04	4.585AspIle: 4.585 ± 0.087	3.067AspLys: 3.067 ± 0.082	5.105AspLeu: 5.105 ± 0.105	1.358AspMet: 1.358 ± 0.044	1.176AspAsn: 1.176 ± 0.054	2.433AspPro: 2.433 ± 0.079	0.944AspGln: 0.944 ± 0.043	2.73AspArg: 2.73 ± 0.069	1.893AspSer: 1.893 ± 0.062	2.318AspThr: 2.318 ± 0.064	4.018AspVal: 4.018 ± 0.089	0.652AspTrp: 0.652 ± 0.034	2.321AspTyr: 2.321 ± 0.069	0.0AspXaa: 0.0 ± 0.0
Glu	4.619GluAla: 4.619 ± 0.094	0.576GluCys: 0.576 ± 0.038	4.675GluAsp: 4.675 ± 0.1	9.195GluGlu: 9.195 ± 0.167	3.29GluPhe: 3.29 ± 0.075	6.316GluGly: 6.316 ± 0.109	1.302GluHis: 1.302 ± 0.049	5.014GluIle: 5.014 ± 0.112	7.344GluLys: 7.344 ± 0.14	8.318GluLeu: 8.318 ± 0.127	1.536GluMet: 1.536 ± 0.053	2.797GluAsn: 2.797 ± 0.078	2.161GluPro: 2.161 ± 0.054	1.606GluGln: 1.606 ± 0.058	5.509GluArg: 5.509 ± 0.12	3.582GluSer: 3.582 ± 0.076	2.503GluThr: 2.503 ± 0.074	6.442GluVal: 6.442 ± 0.114	0.731GluTrp: 0.731 ± 0.035	2.712GluTyr: 2.712 ± 0.07	0.0GluXaa: 0.0 ± 0.0
Phe	2.759PheAla: 2.759 ± 0.089	0.522PheCys: 0.522 ± 0.03	2.246PheAsp: 2.246 ± 0.061	3.229PheGlu: 3.229 ± 0.074	2.653PhePhe: 2.653 ± 0.088	3.393PheGly: 3.393 ± 0.084	0.764PheHis: 0.764 ± 0.037	3.206PheIle: 3.206 ± 0.078	2.901PheLys: 2.901 ± 0.069	6.146PheLeu: 6.146 ± 0.142	1.217PheMet: 1.217 ± 0.048	1.372PheAsn: 1.372 ± 0.054	1.905PhePro: 1.905 ± 0.067	1.054PheGln: 1.054 ± 0.041	2.004PheArg: 2.004 ± 0.063	3.404PheSer: 3.404 ± 0.09	2.066PheThr: 2.066 ± 0.061	3.735PheVal: 3.735 ± 0.078	0.526PheTrp: 0.526 ± 0.033	2.388PheTyr: 2.388 ± 0.07	0.0PheXaa: 0.0 ± 0.0
Gly	4.171GlyAla: 4.171 ± 0.103	0.821GlyCys: 0.821 ± 0.044	3.323GlyAsp: 3.323 ± 0.083	4.729GlyGlu: 4.729 ± 0.111	3.931GlyPhe: 3.931 ± 0.085	5.096GlyGly: 5.096 ± 0.141	1.21GlyHis: 1.21 ± 0.057	6.091GlyIle: 6.091 ± 0.121	5.957GlyLys: 5.957 ± 0.115	6.217GlyLeu: 6.217 ± 0.107	1.767GlyMet: 1.767 ± 0.055	2.19GlyAsn: 2.19 ± 0.061	1.524GlyPro: 1.524 ± 0.062	1.662GlyGln: 1.662 ± 0.058	3.921GlyArg: 3.921 ± 0.095	3.845GlySer: 3.845 ± 0.093	3.24GlyThr: 3.24 ± 0.088	5.651GlyVal: 5.651 ± 0.092	0.877GlyTrp: 0.877 ± 0.038	3.17GlyTyr: 3.17 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.1HisAla: 1.1 ± 0.051	0.202HisCys: 0.202 ± 0.018	0.52HisAsp: 0.52 ± 0.031	0.954HisGlu: 0.954 ± 0.044	0.888HisPhe: 0.888 ± 0.043	1.097HisGly: 1.097 ± 0.049	0.501HisHis: 0.501 ± 0.028	1.633HisIle: 1.633 ± 0.057	0.902HisLys: 0.902 ± 0.043	2.32HisLeu: 2.32 ± 0.071	0.508HisMet: 0.508 ± 0.028	0.495HisAsn: 0.495 ± 0.025	1.198HisPro: 1.198 ± 0.045	0.582HisGln: 0.582 ± 0.032	0.944HisArg: 0.944 ± 0.043	0.872HisSer: 0.872 ± 0.04	0.974HisThr: 0.974 ± 0.044	1.196HisVal: 1.196 ± 0.041	0.175HisTrp: 0.175 ± 0.016	0.737HisTyr: 0.737 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	4.814IleAla: 4.814 ± 0.104	0.558IleCys: 0.558 ± 0.036	3.735IleAsp: 3.735 ± 0.084	5.496IleGlu: 5.496 ± 0.102	2.881IlePhe: 2.881 ± 0.095	4.369IleGly: 4.369 ± 0.095	1.142IleHis: 1.142 ± 0.047	4.14IleIle: 4.14 ± 0.096	6.73IleLys: 6.73 ± 0.106	6.908IleLeu: 6.908 ± 0.119	1.315IleMet: 1.315 ± 0.037	3.015IleAsn: 3.015 ± 0.07	3.272IlePro: 3.272 ± 0.074	1.48IleGln: 1.48 ± 0.049	3.847IleArg: 3.847 ± 0.093	4.436IleSer: 4.436 ± 0.074	4.227IleThr: 4.227 ± 0.079	4.61IleVal: 4.61 ± 0.094	0.461IleTrp: 0.461 ± 0.028	2.77IleTyr: 2.77 ± 0.078	0.0IleXaa: 0.0 ± 0.0
Lys	5.557LysAla: 5.557 ± 0.106	0.402LysCys: 0.402 ± 0.032	5.023LysAsp: 5.023 ± 0.096	8.52LysGlu: 8.52 ± 0.138	2.181LysPhe: 2.181 ± 0.061	5.585LysGly: 5.585 ± 0.112	1.268LysHis: 1.268 ± 0.048	4.311LysIle: 4.311 ± 0.105	6.024LysLys: 6.024 ± 0.116	7.859LysLeu: 7.859 ± 0.14	1.574LysMet: 1.574 ± 0.05	2.755LysAsn: 2.755 ± 0.087	3.254LysPro: 3.254 ± 0.071	1.56LysGln: 1.56 ± 0.059	4.646LysArg: 4.646 ± 0.099	3.991LysSer: 3.991 ± 0.086	3.189LysThr: 3.189 ± 0.061	7.081LysVal: 7.081 ± 0.122	0.582LysTrp: 0.582 ± 0.036	2.559LysTyr: 2.559 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	6.564LeuAla: 6.564 ± 0.117	0.962LeuCys: 0.962 ± 0.042	4.383LeuAsp: 4.383 ± 0.086	8.723LeuGlu: 8.723 ± 0.149	5.143LeuPhe: 5.143 ± 0.126	7.202LeuGly: 7.202 ± 0.134	1.65LeuHis: 1.65 ± 0.056	6.962LeuIle: 6.962 ± 0.133	9.728LeuLys: 9.728 ± 0.149	12.946LeuLeu: 12.946 ± 0.237	2.716LeuMet: 2.716 ± 0.074	3.135LeuAsn: 3.135 ± 0.082	4.688LeuPro: 4.688 ± 0.093	2.764LeuGln: 2.764 ± 0.091	6.049LeuArg: 6.049 ± 0.125	8.759LeuSer: 8.759 ± 0.139	4.852LeuThr: 4.852 ± 0.09	7.263LeuVal: 7.263 ± 0.129	1.124LeuTrp: 1.124 ± 0.045	4.499LeuTyr: 4.499 ± 0.095	0.0LeuXaa: 0.0 ± 0.0
Met	1.713MetAla: 1.713 ± 0.055	0.27MetCys: 0.27 ± 0.022	1.205MetAsp: 1.205 ± 0.045	2.067MetGlu: 2.067 ± 0.07	0.902MetPhe: 0.902 ± 0.044	1.947MetGly: 1.947 ± 0.063	0.439MetHis: 0.439 ± 0.025	1.259MetIle: 1.259 ± 0.047	1.785MetLys: 1.785 ± 0.055	2.66MetLeu: 2.66 ± 0.077	0.484MetMet: 0.484 ± 0.031	0.724MetAsn: 0.724 ± 0.03	1.075MetPro: 1.075 ± 0.041	0.634MetGln: 0.634 ± 0.033	1.367MetArg: 1.367 ± 0.044	1.455MetSer: 1.455 ± 0.05	0.828MetThr: 0.828 ± 0.036	1.759MetVal: 1.759 ± 0.06	0.279MetTrp: 0.279 ± 0.019	0.683MetTyr: 0.683 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	2.152AsnAla: 2.152 ± 0.064	0.402AsnCys: 0.402 ± 0.032	0.927AsnAsp: 0.927 ± 0.039	1.493AsnGlu: 1.493 ± 0.055	1.713AsnPhe: 1.713 ± 0.057	1.619AsnGly: 1.619 ± 0.062	0.542AsnHis: 0.542 ± 0.028	2.694AsnIle: 2.694 ± 0.069	1.783AsnLys: 1.783 ± 0.065	4.212AsnLeu: 4.212 ± 0.093	0.823AsnMet: 0.823 ± 0.04	0.893AsnAsn: 0.893 ± 0.049	2.278AsnPro: 2.278 ± 0.077	0.834AsnGln: 0.834 ± 0.041	1.653AsnArg: 1.653 ± 0.063	1.65AsnSer: 1.65 ± 0.058	1.704AsnThr: 1.704 ± 0.058	2.163AsnVal: 2.163 ± 0.066	0.411AsnTrp: 0.411 ± 0.029	1.583AsnTyr: 1.583 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.442ProAla: 2.442 ± 0.078	0.366ProCys: 0.366 ± 0.025	2.123ProAsp: 2.123 ± 0.065	3.595ProGlu: 3.595 ± 0.089	2.15ProPhe: 2.15 ± 0.069	2.046ProGly: 2.046 ± 0.076	0.963ProHis: 0.963 ± 0.038	2.64ProIle: 2.64 ± 0.076	2.795ProLys: 2.795 ± 0.077	3.993ProLeu: 3.993 ± 0.086	0.965ProMet: 0.965 ± 0.04	1.399ProAsn: 1.399 ± 0.047	1.9ProPro: 1.9 ± 0.073	1.432ProGln: 1.432 ± 0.055	1.414ProArg: 1.414 ± 0.059	2.501ProSer: 2.501 ± 0.069	2.006ProThr: 2.006 ± 0.054	3.159ProVal: 3.159 ± 0.074	0.405ProTrp: 0.405 ± 0.027	2.082ProTyr: 2.082 ± 0.063	0.0ProXaa: 0.0 ± 0.0
Gln	2.004GlnAla: 2.004 ± 0.066	0.198GlnCys: 0.198 ± 0.021	1.331GlnAsp: 1.331 ± 0.046	2.851GlnGlu: 2.851 ± 0.07	0.733GlnPhe: 0.733 ± 0.039	1.435GlnGly: 1.435 ± 0.06	0.425GlnHis: 0.425 ± 0.03	1.929GlnIle: 1.929 ± 0.062	2.521GlnLys: 2.521 ± 0.076	2.201GlnLeu: 2.201 ± 0.066	0.785GlnMet: 0.785 ± 0.038	0.92GlnAsn: 0.92 ± 0.043	0.801GlnPro: 0.801 ± 0.043	0.812GlnGln: 0.812 ± 0.048	1.61GlnArg: 1.61 ± 0.049	1.153GlnSer: 1.153 ± 0.042	1.185GlnThr: 1.185 ± 0.051	2.303GlnVal: 2.303 ± 0.066	0.268GlnTrp: 0.268 ± 0.027	0.726GlnTyr: 0.726 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	3.323ArgAla: 3.323 ± 0.082	0.513ArgCys: 0.513 ± 0.036	2.93ArgAsp: 2.93 ± 0.078	5.539ArgGlu: 5.539 ± 0.114	2.392ArgPhe: 2.392 ± 0.069	3.69ArgGly: 3.69 ± 0.097	0.963ArgHis: 0.963 ± 0.048	4.128ArgIle: 4.128 ± 0.093	3.937ArgLys: 3.937 ± 0.084	5.621ArgLeu: 5.621 ± 0.103	1.311ArgMet: 1.311 ± 0.049	1.693ArgAsn: 1.693 ± 0.057	1.424ArgPro: 1.424 ± 0.054	1.43ArgGln: 1.43 ± 0.048	3.27ArgArg: 3.27 ± 0.089	2.609ArgSer: 2.609 ± 0.064	1.849ArgThr: 1.849 ± 0.056	4.355ArgVal: 4.355 ± 0.087	0.728ArgTrp: 0.728 ± 0.042	2.321ArgTyr: 2.321 ± 0.063	0.0ArgXaa: 0.0 ± 0.0
Ser	4.07SerAla: 4.07 ± 0.093	0.719SerCys: 0.719 ± 0.039	2.275SerAsp: 2.275 ± 0.063	3.58SerGlu: 3.58 ± 0.082	3.564SerPhe: 3.564 ± 0.092	3.856SerGly: 3.856 ± 0.085	1.077SerHis: 1.077 ± 0.048	4.086SerIle: 4.086 ± 0.089	4.025SerLys: 4.025 ± 0.08	7.8SerLeu: 7.8 ± 0.14	1.585SerMet: 1.585 ± 0.046	1.502SerAsn: 1.502 ± 0.058	2.442SerPro: 2.442 ± 0.075	1.851SerGln: 1.851 ± 0.059	2.633SerArg: 2.633 ± 0.068	3.697SerSer: 3.697 ± 0.104	2.368SerThr: 2.368 ± 0.072	4.304SerVal: 4.304 ± 0.078	0.661SerTrp: 0.661 ± 0.033	2.851SerTyr: 2.851 ± 0.077	0.0SerXaa: 0.0 ± 0.0
Thr	2.957ThrAla: 2.957 ± 0.079	0.465ThrCys: 0.465 ± 0.034	2.165ThrAsp: 2.165 ± 0.059	2.354ThrGlu: 2.354 ± 0.069	2.348ThrPhe: 2.348 ± 0.066	3.542ThrGly: 3.542 ± 0.086	0.924ThrHis: 0.924 ± 0.04	2.442ThrIle: 2.442 ± 0.071	2.838ThrLys: 2.838 ± 0.078	5.08ThrLeu: 5.08 ± 0.101	0.76ThrMet: 0.76 ± 0.04	1.253ThrAsn: 1.253 ± 0.047	2.302ThrPro: 2.302 ± 0.067	1.273ThrGln: 1.273 ± 0.055	1.696ThrArg: 1.696 ± 0.06	2.451ThrSer: 2.451 ± 0.066	1.833ThrThr: 1.833 ± 0.066	3.191ThrVal: 3.191 ± 0.077	0.337ThrTrp: 0.337 ± 0.026	1.976ThrTyr: 1.976 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	4.877ValAla: 4.877 ± 0.089	1.014ValCys: 1.014 ± 0.052	3.647ValAsp: 3.647 ± 0.094	6.004ValGlu: 6.004 ± 0.108	3.917ValPhe: 3.917 ± 0.081	5.68ValGly: 5.68 ± 0.114	1.203ValHis: 1.203 ± 0.052	5.752ValIle: 5.752 ± 0.105	6.328ValLys: 6.328 ± 0.105	8.369ValLeu: 8.369 ± 0.137	1.716ValMet: 1.716 ± 0.059	2.39ValAsn: 2.39 ± 0.067	3.162ValPro: 3.162 ± 0.074	1.804ValGln: 1.804 ± 0.059	4.081ValArg: 4.081 ± 0.093	5.358ValSer: 5.358 ± 0.106	1.419ValThr: 1.419 ± 0.055	6.172ValVal: 6.172 ± 0.091	0.864ValTrp: 0.864 ± 0.047	3.177ValTyr: 3.177 ± 0.073	0.0ValXaa: 0.0 ± 0.0
Trp	0.6TrpAla: 0.6 ± 0.034	0.097TrpCys: 0.097 ± 0.015	0.61TrpAsp: 0.61 ± 0.034	0.94TrpGlu: 0.94 ± 0.042	0.452TrpPhe: 0.452 ± 0.029	0.724TrpGly: 0.724 ± 0.039	0.243TrpHis: 0.243 ± 0.021	0.764TrpIle: 0.764 ± 0.043	0.837TrpLys: 0.837 ± 0.04	1.055TrpLeu: 1.055 ± 0.045	0.292TrpMet: 0.292 ± 0.025	0.373TrpAsn: 0.373 ± 0.027	0.157TrpPro: 0.157 ± 0.016	0.349TrpGln: 0.349 ± 0.023	0.6TrpArg: 0.6 ± 0.036	0.502TrpSer: 0.502 ± 0.035	0.378TrpThr: 0.378 ± 0.024	0.807TrpVal: 0.807 ± 0.04	0.14TrpTrp: 0.14 ± 0.018	0.355TrpTyr: 0.355 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.101TyrAla: 3.101 ± 0.082	0.391TyrCys: 0.391 ± 0.027	1.909TyrAsp: 1.909 ± 0.056	3.054TyrGlu: 3.054 ± 0.076	1.81TyrPhe: 1.81 ± 0.055	2.962TyrGly: 2.962 ± 0.077	0.904TyrHis: 0.904 ± 0.041	2.95TyrIle: 2.95 ± 0.064	2.741TyrLys: 2.741 ± 0.069	4.517TyrLeu: 4.517 ± 0.097	1.007TyrMet: 1.007 ± 0.04	1.289TyrAsn: 1.289 ± 0.048	1.835TyrPro: 1.835 ± 0.055	1.403TyrGln: 1.403 ± 0.053	2.743TyrArg: 2.743 ± 0.061	2.521TyrSer: 2.521 ± 0.063	2.275TyrThr: 2.275 ± 0.067	2.647TyrVal: 2.647 ± 0.068	0.416TyrTrp: 0.416 ± 0.03	1.833TyrTyr: 1.833 ± 0.063	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1892 proteins (555287 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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