Amino acid dipepetide frequency for Microbacterium phage Warren

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.283AlaAla: 17.283 ± 1.646
0.4AlaCys: 0.4 ± 0.185
9.041AlaAsp: 9.041 ± 0.801
9.922AlaGlu: 9.922 ± 0.821
2.56AlaPhe: 2.56 ± 0.582
8.641AlaGly: 8.641 ± 0.97
2.0AlaHis: 2.0 ± 0.364
6.401AlaIle: 6.401 ± 0.666
4.881AlaLys: 4.881 ± 0.77
12.402AlaLeu: 12.402 ± 1.024
3.04AlaMet: 3.04 ± 0.46
3.361AlaAsn: 3.361 ± 0.45
5.921AlaPro: 5.921 ± 0.955
4.081AlaGln: 4.081 ± 0.583
9.522AlaArg: 9.522 ± 1.268
6.881AlaSer: 6.881 ± 0.712
6.961AlaThr: 6.961 ± 0.525
8.321AlaVal: 8.321 ± 1.04
2.48AlaTrp: 2.48 ± 0.452
2.24AlaTyr: 2.24 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.72CysAla: 0.72 ± 0.331
0.0CysCys: 0.0 ± 0.0
0.64CysAsp: 0.64 ± 0.278
0.32CysGlu: 0.32 ± 0.148
0.0CysPhe: 0.0 ± 0.0
0.96CysGly: 0.96 ± 0.289
0.32CysHis: 0.32 ± 0.151
0.16CysIle: 0.16 ± 0.108
0.16CysLys: 0.16 ± 0.102
0.16CysLeu: 0.16 ± 0.115
0.08CysMet: 0.08 ± 0.075
0.0CysAsn: 0.0 ± 0.0
0.4CysPro: 0.4 ± 0.162
0.08CysGln: 0.08 ± 0.083
0.56CysArg: 0.56 ± 0.235
0.24CysSer: 0.24 ± 0.132
0.0CysThr: 0.0 ± 0.0
0.4CysVal: 0.4 ± 0.201
0.16CysTrp: 0.16 ± 0.119
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
10.242AspAla: 10.242 ± 0.78
0.16AspCys: 0.16 ± 0.116
5.281AspAsp: 5.281 ± 0.785
6.081AspGlu: 6.081 ± 0.653
1.68AspPhe: 1.68 ± 0.356
6.641AspGly: 6.641 ± 0.798
1.12AspHis: 1.12 ± 0.322
2.8AspIle: 2.8 ± 0.497
2.16AspLys: 2.16 ± 0.376
5.681AspLeu: 5.681 ± 0.976
1.2AspMet: 1.2 ± 0.33
1.12AspAsn: 1.12 ± 0.302
3.361AspPro: 3.361 ± 0.72
2.08AspGln: 2.08 ± 0.408
4.241AspArg: 4.241 ± 0.538
3.361AspSer: 3.361 ± 0.594
3.201AspThr: 3.201 ± 0.544
5.841AspVal: 5.841 ± 0.915
1.2AspTrp: 1.2 ± 0.257
1.28AspTyr: 1.28 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
8.241GluAla: 8.241 ± 0.789
0.4GluCys: 0.4 ± 0.22
3.281GluAsp: 3.281 ± 0.516
1.12GluGlu: 1.12 ± 0.319
2.72GluPhe: 2.72 ± 0.425
4.721GluGly: 4.721 ± 0.589
2.72GluHis: 2.72 ± 0.507
4.801GluIle: 4.801 ± 0.578
1.12GluLys: 1.12 ± 0.328
4.321GluLeu: 4.321 ± 0.493
2.32GluMet: 2.32 ± 0.38
2.08GluAsn: 2.08 ± 0.348
3.921GluPro: 3.921 ± 0.657
3.441GluGln: 3.441 ± 0.569
6.881GluArg: 6.881 ± 0.8
4.161GluSer: 4.161 ± 0.564
4.481GluThr: 4.481 ± 0.71
2.96GluVal: 2.96 ± 0.578
1.04GluTrp: 1.04 ± 0.311
1.68GluTyr: 1.68 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
3.201PheAla: 3.201 ± 0.433
0.08PheCys: 0.08 ± 0.084
2.64PheAsp: 2.64 ± 0.424
2.0PheGlu: 2.0 ± 0.393
0.64PhePhe: 0.64 ± 0.383
3.12PheGly: 3.12 ± 0.549
0.24PheHis: 0.24 ± 0.143
1.44PheIle: 1.44 ± 0.462
0.72PheLys: 0.72 ± 0.205
1.2PheLeu: 1.2 ± 0.412
0.56PheMet: 0.56 ± 0.199
0.4PheAsn: 0.4 ± 0.16
0.32PhePro: 0.32 ± 0.152
0.8PheGln: 0.8 ± 0.193
1.52PheArg: 1.52 ± 0.422
1.6PheSer: 1.6 ± 0.438
2.0PheThr: 2.0 ± 0.363
2.4PheVal: 2.4 ± 0.342
0.4PheTrp: 0.4 ± 0.217
0.56PheTyr: 0.56 ± 0.22
0.0PheXaa: 0.0 ± 0.0
Gly
9.281GlyAla: 9.281 ± 1.021
0.48GlyCys: 0.48 ± 0.199
5.521GlyAsp: 5.521 ± 0.756
6.081GlyGlu: 6.081 ± 0.841
2.48GlyPhe: 2.48 ± 0.784
7.041GlyGly: 7.041 ± 0.787
1.2GlyHis: 1.2 ± 0.282
4.401GlyIle: 4.401 ± 0.887
2.24GlyLys: 2.24 ± 0.368
6.401GlyLeu: 6.401 ± 1.024
2.0GlyMet: 2.0 ± 0.61
2.56GlyAsn: 2.56 ± 0.452
3.521GlyPro: 3.521 ± 0.566
2.0GlyGln: 2.0 ± 0.334
6.001GlyArg: 6.001 ± 0.752
5.761GlySer: 5.761 ± 0.947
6.721GlyThr: 6.721 ± 0.946
6.801GlyVal: 6.801 ± 0.678
1.92GlyTrp: 1.92 ± 0.372
2.08GlyTyr: 2.08 ± 0.484
0.0GlyXaa: 0.0 ± 0.0
His
2.24HisAla: 2.24 ± 0.508
0.08HisCys: 0.08 ± 0.069
1.28HisAsp: 1.28 ± 0.354
1.52HisGlu: 1.52 ± 0.384
0.64HisPhe: 0.64 ± 0.231
1.44HisGly: 1.44 ± 0.334
0.64HisHis: 0.64 ± 0.225
0.64HisIle: 0.64 ± 0.205
0.4HisLys: 0.4 ± 0.171
1.36HisLeu: 1.36 ± 0.399
0.4HisMet: 0.4 ± 0.178
0.24HisAsn: 0.24 ± 0.136
1.36HisPro: 1.36 ± 0.324
0.48HisGln: 0.48 ± 0.197
1.36HisArg: 1.36 ± 0.375
0.96HisSer: 0.96 ± 0.272
0.72HisThr: 0.72 ± 0.254
1.52HisVal: 1.52 ± 0.331
0.32HisTrp: 0.32 ± 0.188
0.32HisTyr: 0.32 ± 0.147
0.0HisXaa: 0.0 ± 0.0
Ile
6.481IleAla: 6.481 ± 0.725
0.16IleCys: 0.16 ± 0.108
4.641IleAsp: 4.641 ± 0.638
5.201IleGlu: 5.201 ± 0.489
1.28IlePhe: 1.28 ± 0.336
4.001IleGly: 4.001 ± 0.782
0.56IleHis: 0.56 ± 0.229
1.92IleIle: 1.92 ± 0.368
1.52IleLys: 1.52 ± 0.348
1.12IleLeu: 1.12 ± 0.3
1.04IleMet: 1.04 ± 0.278
0.64IleAsn: 0.64 ± 0.242
1.36IlePro: 1.36 ± 0.323
1.6IleGln: 1.6 ± 0.388
3.12IleArg: 3.12 ± 0.485
1.92IleSer: 1.92 ± 0.381
3.761IleThr: 3.761 ± 0.543
5.521IleVal: 5.521 ± 0.727
0.48IleTrp: 0.48 ± 0.218
1.12IleTyr: 1.12 ± 0.335
0.0IleXaa: 0.0 ± 0.0
Lys
4.161LysAla: 4.161 ± 0.552
0.08LysCys: 0.08 ± 0.084
0.72LysAsp: 0.72 ± 0.271
1.2LysGlu: 1.2 ± 0.308
0.96LysPhe: 0.96 ± 0.312
2.08LysGly: 2.08 ± 0.472
0.8LysHis: 0.8 ± 0.229
2.32LysIle: 2.32 ± 0.484
0.96LysLys: 0.96 ± 0.284
0.72LysLeu: 0.72 ± 0.266
0.96LysMet: 0.96 ± 0.283
0.32LysAsn: 0.32 ± 0.142
2.16LysPro: 2.16 ± 0.511
0.64LysGln: 0.64 ± 0.247
3.681LysArg: 3.681 ± 0.603
1.76LysSer: 1.76 ± 0.399
3.04LysThr: 3.04 ± 0.492
2.96LysVal: 2.96 ± 0.424
0.4LysTrp: 0.4 ± 0.165
0.56LysTyr: 0.56 ± 0.182
0.0LysXaa: 0.0 ± 0.0
Leu
11.202LeuAla: 11.202 ± 1.244
0.64LeuCys: 0.64 ± 0.269
6.561LeuAsp: 6.561 ± 0.737
2.64LeuGlu: 2.64 ± 0.445
1.92LeuPhe: 1.92 ± 0.459
6.081LeuGly: 6.081 ± 0.673
0.64LeuHis: 0.64 ± 0.263
3.12LeuIle: 3.12 ± 0.486
2.16LeuLys: 2.16 ± 0.404
6.961LeuLeu: 6.961 ± 0.77
1.28LeuMet: 1.28 ± 0.423
1.68LeuAsn: 1.68 ± 0.415
3.441LeuPro: 3.441 ± 0.56
1.28LeuGln: 1.28 ± 0.313
5.921LeuArg: 5.921 ± 0.729
5.201LeuSer: 5.201 ± 0.636
5.601LeuThr: 5.601 ± 0.634
6.321LeuVal: 6.321 ± 0.675
1.2LeuTrp: 1.2 ± 0.339
1.76LeuTyr: 1.76 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
1.84MetAla: 1.84 ± 0.417
0.0MetCys: 0.0 ± 0.0
0.4MetAsp: 0.4 ± 0.161
0.8MetGlu: 0.8 ± 0.198
0.56MetPhe: 0.56 ± 0.264
1.12MetGly: 1.12 ± 0.485
0.24MetHis: 0.24 ± 0.132
1.44MetIle: 1.44 ± 0.335
0.4MetLys: 0.4 ± 0.169
2.16MetLeu: 2.16 ± 0.431
0.64MetMet: 0.64 ± 0.334
0.8MetAsn: 0.8 ± 0.278
2.16MetPro: 2.16 ± 0.456
1.52MetGln: 1.52 ± 0.339
2.4MetArg: 2.4 ± 0.46
3.761MetSer: 3.761 ± 0.583
2.96MetThr: 2.96 ± 0.443
1.04MetVal: 1.04 ± 0.264
0.4MetTrp: 0.4 ± 0.147
0.32MetTyr: 0.32 ± 0.147
0.0MetXaa: 0.0 ± 0.0
Asn
3.601AsnAla: 3.601 ± 0.572
0.24AsnCys: 0.24 ± 0.144
1.84AsnAsp: 1.84 ± 0.417
1.36AsnGlu: 1.36 ± 0.337
0.4AsnPhe: 0.4 ± 0.192
4.001AsnGly: 4.001 ± 0.739
0.24AsnHis: 0.24 ± 0.13
0.88AsnIle: 0.88 ± 0.244
0.64AsnLys: 0.64 ± 0.217
1.92AsnLeu: 1.92 ± 0.379
0.16AsnMet: 0.16 ± 0.122
0.32AsnAsn: 0.32 ± 0.133
1.2AsnPro: 1.2 ± 0.283
0.48AsnGln: 0.48 ± 0.206
2.0AsnArg: 2.0 ± 0.426
1.12AsnSer: 1.12 ± 0.253
1.44AsnThr: 1.44 ± 0.312
1.76AsnVal: 1.76 ± 0.302
0.56AsnTrp: 0.56 ± 0.182
0.32AsnTyr: 0.32 ± 0.241
0.0AsnXaa: 0.0 ± 0.0
Pro
5.041ProAla: 5.041 ± 0.937
0.16ProCys: 0.16 ± 0.107
4.001ProAsp: 4.001 ± 0.701
3.361ProGlu: 3.361 ± 0.522
1.04ProPhe: 1.04 ± 0.291
5.681ProGly: 5.681 ± 1.043
0.72ProHis: 0.72 ± 0.249
1.68ProIle: 1.68 ± 0.345
1.68ProLys: 1.68 ± 0.341
2.88ProLeu: 2.88 ± 0.586
2.0ProMet: 2.0 ± 0.406
1.6ProAsn: 1.6 ± 0.285
2.72ProPro: 2.72 ± 0.617
1.2ProGln: 1.2 ± 0.358
2.96ProArg: 2.96 ± 0.511
3.12ProSer: 3.12 ± 0.544
4.321ProThr: 4.321 ± 0.659
4.881ProVal: 4.881 ± 0.61
0.64ProTrp: 0.64 ± 0.236
0.96ProTyr: 0.96 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
3.04GlnAla: 3.04 ± 0.484
0.0GlnCys: 0.0 ± 0.0
1.2GlnAsp: 1.2 ± 0.295
1.52GlnGlu: 1.52 ± 0.405
1.04GlnPhe: 1.04 ± 0.281
2.24GlnGly: 2.24 ± 0.517
1.04GlnHis: 1.04 ± 0.289
1.28GlnIle: 1.28 ± 0.289
0.48GlnLys: 0.48 ± 0.159
0.8GlnLeu: 0.8 ± 0.237
1.04GlnMet: 1.04 ± 0.271
0.88GlnAsn: 0.88 ± 0.224
2.72GlnPro: 2.72 ± 0.559
1.76GlnGln: 1.76 ± 0.486
3.681GlnArg: 3.681 ± 0.559
2.08GlnSer: 2.08 ± 0.317
1.52GlnThr: 1.52 ± 0.414
2.88GlnVal: 2.88 ± 0.543
0.96GlnTrp: 0.96 ± 0.266
1.36GlnTyr: 1.36 ± 0.333
0.0GlnXaa: 0.0 ± 0.0
Arg
9.762ArgAla: 9.762 ± 1.112
0.88ArgCys: 0.88 ± 0.331
5.681ArgAsp: 5.681 ± 0.601
6.321ArgGlu: 6.321 ± 0.797
1.44ArgPhe: 1.44 ± 0.406
5.601ArgGly: 5.601 ± 0.641
1.44ArgHis: 1.44 ± 0.346
2.8ArgIle: 2.8 ± 0.448
2.64ArgLys: 2.64 ± 0.434
7.041ArgLeu: 7.041 ± 0.654
2.4ArgMet: 2.4 ± 0.405
2.16ArgAsn: 2.16 ± 0.418
3.12ArgPro: 3.12 ± 0.531
2.56ArgGln: 2.56 ± 0.423
7.041ArgArg: 7.041 ± 0.802
3.201ArgSer: 3.201 ± 0.494
3.841ArgThr: 3.841 ± 0.57
5.281ArgVal: 5.281 ± 0.639
1.52ArgTrp: 1.52 ± 0.308
2.48ArgTyr: 2.48 ± 0.524
0.0ArgXaa: 0.0 ± 0.0
Ser
6.801SerAla: 6.801 ± 0.675
0.08SerCys: 0.08 ± 0.08
3.12SerAsp: 3.12 ± 0.525
2.88SerGlu: 2.88 ± 0.401
2.08SerPhe: 2.08 ± 0.43
6.641SerGly: 6.641 ± 0.962
0.8SerHis: 0.8 ± 0.266
3.201SerIle: 3.201 ± 0.547
2.32SerLys: 2.32 ± 0.483
4.721SerLeu: 4.721 ± 0.685
2.16SerMet: 2.16 ± 0.485
1.2SerAsn: 1.2 ± 0.252
2.96SerPro: 2.96 ± 0.472
1.68SerGln: 1.68 ± 0.387
3.201SerArg: 3.201 ± 0.462
2.96SerSer: 2.96 ± 0.477
4.401SerThr: 4.401 ± 0.613
4.481SerVal: 4.481 ± 0.402
1.12SerTrp: 1.12 ± 0.265
1.04SerTyr: 1.04 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
8.161ThrAla: 8.161 ± 0.988
0.64ThrCys: 0.64 ± 0.255
4.001ThrAsp: 4.001 ± 0.558
4.401ThrGlu: 4.401 ± 0.487
2.32ThrPhe: 2.32 ± 0.45
5.041ThrGly: 5.041 ± 0.563
0.96ThrHis: 0.96 ± 0.294
2.72ThrIle: 2.72 ± 0.462
2.8ThrLys: 2.8 ± 0.365
6.001ThrLeu: 6.001 ± 0.617
1.2ThrMet: 1.2 ± 0.325
1.52ThrAsn: 1.52 ± 0.342
4.641ThrPro: 4.641 ± 0.532
2.24ThrGln: 2.24 ± 0.41
4.161ThrArg: 4.161 ± 0.52
3.201ThrSer: 3.201 ± 0.524
4.401ThrThr: 4.401 ± 0.646
4.641ThrVal: 4.641 ± 0.525
2.08ThrTrp: 2.08 ± 0.475
1.28ThrTyr: 1.28 ± 0.272
0.0ThrXaa: 0.0 ± 0.0
Val
9.041ValAla: 9.041 ± 0.763
0.56ValCys: 0.56 ± 0.194
6.561ValAsp: 6.561 ± 0.767
5.601ValGlu: 5.601 ± 0.648
1.2ValPhe: 1.2 ± 0.305
6.561ValGly: 6.561 ± 0.815
1.12ValHis: 1.12 ± 0.293
3.361ValIle: 3.361 ± 0.424
2.4ValLys: 2.4 ± 0.394
6.721ValLeu: 6.721 ± 0.783
1.36ValMet: 1.36 ± 0.32
2.24ValAsn: 2.24 ± 0.256
3.921ValPro: 3.921 ± 0.537
2.48ValGln: 2.48 ± 0.346
5.681ValArg: 5.681 ± 0.725
4.241ValSer: 4.241 ± 0.597
5.681ValThr: 5.681 ± 0.73
4.801ValVal: 4.801 ± 0.674
1.2ValTrp: 1.2 ± 0.353
2.0ValTyr: 2.0 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
2.4TrpAla: 2.4 ± 0.401
0.16TrpCys: 0.16 ± 0.107
1.04TrpAsp: 1.04 ± 0.245
2.16TrpGlu: 2.16 ± 0.408
0.4TrpPhe: 0.4 ± 0.193
0.96TrpGly: 0.96 ± 0.25
0.8TrpHis: 0.8 ± 0.253
1.6TrpIle: 1.6 ± 0.5
0.4TrpLys: 0.4 ± 0.184
1.6TrpLeu: 1.6 ± 0.391
0.48TrpMet: 0.48 ± 0.22
0.8TrpAsn: 0.8 ± 0.399
0.72TrpPro: 0.72 ± 0.251
0.32TrpGln: 0.32 ± 0.139
1.6TrpArg: 1.6 ± 0.318
1.04TrpSer: 1.04 ± 0.311
0.72TrpThr: 0.72 ± 0.243
0.88TrpVal: 0.88 ± 0.249
0.4TrpTrp: 0.4 ± 0.2
0.48TrpTyr: 0.48 ± 0.252
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.601TyrAla: 3.601 ± 0.426
0.16TyrCys: 0.16 ± 0.105
1.28TyrAsp: 1.28 ± 0.388
1.92TyrGlu: 1.92 ± 0.425
0.48TyrPhe: 0.48 ± 0.188
2.0TyrGly: 2.0 ± 0.486
0.32TyrHis: 0.32 ± 0.169
0.48TyrIle: 0.48 ± 0.178
0.4TyrLys: 0.4 ± 0.17
1.44TyrLeu: 1.44 ± 0.341
0.56TyrMet: 0.56 ± 0.212
0.56TyrAsn: 0.56 ± 0.212
0.72TyrPro: 0.72 ± 0.213
0.8TyrGln: 0.8 ± 0.262
1.68TyrArg: 1.68 ± 0.484
1.28TyrSer: 1.28 ± 0.32
0.64TyrThr: 0.64 ± 0.243
3.04TyrVal: 3.04 ± 0.465
0.48TyrTrp: 0.48 ± 0.169
0.72TyrTyr: 0.72 ± 0.256
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12499 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski