Pelagibacter phage HTVC025P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; unclassified Autographiviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NU62|A0A4Y1NU62_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC025P OX=2259657 GN=P025_gp38 PE=4 SV=1
MM1 pKa = 7.49SFLAQVSYY9 pKa = 10.18TGNGSTTQYY18 pKa = 10.87SITFPYY24 pKa = 10.18IDD26 pKa = 3.6VTHH29 pKa = 6.27VKK31 pKa = 10.38AYY33 pKa = 11.1LNGTLTSAYY42 pKa = 8.52TISSSTLTFTTAPANGVVIRR62 pKa = 11.84IEE64 pKa = 4.06RR65 pKa = 11.84EE66 pKa = 3.93TPNDD70 pKa = 3.04NRR72 pKa = 11.84LVDD75 pKa = 4.18FTDD78 pKa = 4.42GSVLTEE84 pKa = 3.73QDD86 pKa = 3.31LDD88 pKa = 3.76RR89 pKa = 11.84SADD92 pKa = 3.46QNFYY96 pKa = 10.27IAQEE100 pKa = 4.05ITDD103 pKa = 4.25DD104 pKa = 3.98SASKK108 pKa = 10.76LGLDD112 pKa = 4.38TDD114 pKa = 4.43DD115 pKa = 5.25KK116 pKa = 11.8YY117 pKa = 11.6NAQSKK122 pKa = 9.09VIKK125 pKa = 10.64NLANPVNDD133 pKa = 3.31TDD135 pKa = 4.1AVNKK139 pKa = 9.93QFISTNLPNINTVAGISANVTTVAGISGNVTTVAGNNANVSTVATNIASVNTVATNIADD198 pKa = 4.21VITVANDD205 pKa = 3.15LNEE208 pKa = 4.92AISEE212 pKa = 4.25IEE214 pKa = 3.99TTANDD219 pKa = 3.39LNEE222 pKa = 4.41AVSEE226 pKa = 4.01IDD228 pKa = 3.48TVGTNIASVQAVGTDD243 pKa = 2.98IANVNTVATDD253 pKa = 3.46IANVNTVATDD263 pKa = 3.46IANVNTVGGAIANVNTVAGANANITTVANANANINTVATDD303 pKa = 3.54IANVNLVGGSIANVNTVATNVANVNTVASNNANINTVASANANITTLAGISADD356 pKa = 2.88ITTVATNSANISTIATDD373 pKa = 3.15IAKK376 pKa = 10.54VITTANDD383 pKa = 3.26LNEE386 pKa = 4.31ATSEE390 pKa = 4.14IEE392 pKa = 4.19VVANAIANVDD402 pKa = 3.29IVGTDD407 pKa = 2.94IADD410 pKa = 3.74VNTVATNIANINAVNSNSSNINAVNSNSANINTVATNDD448 pKa = 3.46ANITTVAGSIANVNTVGSNIGTVNEE473 pKa = 3.84FGEE476 pKa = 4.71RR477 pKa = 11.84YY478 pKa = 9.02RR479 pKa = 11.84VSASAPSTSLDD490 pKa = 3.96LGDD493 pKa = 5.46LYY495 pKa = 11.35FDD497 pKa = 3.82TASNTMKK504 pKa = 10.34VYY506 pKa = 10.95SSGGWINAGSSVNGTADD523 pKa = 3.5RR524 pKa = 11.84YY525 pKa = 10.78KK526 pKa = 9.54YY527 pKa = 8.79TATASQTTFTGADD540 pKa = 3.54DD541 pKa = 3.94NGNTLGYY548 pKa = 10.3DD549 pKa = 3.07AGFLDD554 pKa = 4.67VYY556 pKa = 11.11LNGIRR561 pKa = 11.84LVNGSDD567 pKa = 3.66FTASSGSSIVLTTGASVSDD586 pKa = 3.5ILEE589 pKa = 3.99VVAFGTFQLANFSITDD605 pKa = 3.76ANDD608 pKa = 3.15VPPLGSAGQALVVNSGGTALEE629 pKa = 4.08FANASSAEE637 pKa = 4.19VYY639 pKa = 10.65GFSRR643 pKa = 11.84DD644 pKa = 3.22INGNLIVTTTNQGQDD659 pKa = 3.27SITQSEE665 pKa = 4.84YY666 pKa = 11.53ANFDD670 pKa = 3.59DD671 pKa = 5.21VLFSASGFTFSISNGEE687 pKa = 4.34LIATII692 pKa = 4.25

Molecular weight: 70.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NU31|A0A4Y1NU31_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC025P OX=2259657 GN=P025_gp10 PE=4 SV=1
MM1 pKa = 7.8CNFKK5 pKa = 10.91APSPPPAPEE14 pKa = 5.26PIPEE18 pKa = 4.24TPPVVTQATTTQKK31 pKa = 11.16APATARR37 pKa = 11.84NEE39 pKa = 4.19SGVNRR44 pKa = 11.84NMASEE49 pKa = 4.53VSRR52 pKa = 11.84KK53 pKa = 8.8RR54 pKa = 11.84QGRR57 pKa = 11.84GSLRR61 pKa = 11.84IPLASLGSGSGLNFPTAA78 pKa = 3.57

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11728

40

1246

239.3

26.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.855 ± 0.525

0.929 ± 0.187

5.585 ± 0.251

6.344 ± 0.451

4.221 ± 0.326

6.804 ± 0.779

1.228 ± 0.213

6.949 ± 0.32

8.245 ± 0.76

8.058 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.918 ± 0.197

6.693 ± 0.48

2.993 ± 0.235

4.246 ± 0.257

4.033 ± 0.266

7.171 ± 0.547

6.881 ± 0.71

6.003 ± 0.355

0.946 ± 0.118

3.897 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski