Microbacterium phage RubyRalph

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XLY0|A0A6G6XLY0_9CAUD Tape measure protein OS=Microbacterium phage RubyRalph OX=2704040 GN=38 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84KK3 pKa = 9.41EE4 pKa = 3.91IKK6 pKa = 10.46ASAGLIAAGAVVAGSVIGASVWIAALAAGDD36 pKa = 4.3LRR38 pKa = 11.84EE39 pKa = 4.76CAYY42 pKa = 9.56PDD44 pKa = 3.57NGSCIVDD51 pKa = 3.57HH52 pKa = 6.77NGEE55 pKa = 4.23GPAKK59 pKa = 10.57DD60 pKa = 4.01SAPLDD65 pKa = 3.39ARR67 pKa = 11.84MPAVTVTSPPVVTALPVVSLASADD91 pKa = 3.92SLPPCPTEE99 pKa = 5.24DD100 pKa = 5.0SEE102 pKa = 4.72GCYY105 pKa = 9.68WDD107 pKa = 5.21ASEE110 pKa = 5.15QGNGDD115 pKa = 3.45GADD118 pKa = 3.2VVTPVPEE125 pKa = 4.07TAPEE129 pKa = 4.1VPAPVTEE136 pKa = 4.39WAPRR140 pKa = 11.84PADD143 pKa = 3.6TEE145 pKa = 4.17RR146 pKa = 11.84ATGPVQEE153 pKa = 4.37YY154 pKa = 11.03DD155 pKa = 3.49EE156 pKa = 5.51GVLFCGVGAKK166 pKa = 9.39PALDD170 pKa = 3.45VDD172 pKa = 4.56PFGNWWAYY180 pKa = 10.34CEE182 pKa = 4.24PALIDD187 pKa = 3.43

Molecular weight: 19.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XLN9|A0A6G6XLN9_9CAUD Uncharacterized protein OS=Microbacterium phage RubyRalph OX=2704040 GN=11 PE=4 SV=1
MM1 pKa = 7.54TPQDD5 pKa = 4.61DD6 pKa = 4.02PVLTPVEE13 pKa = 3.73AAAAIRR19 pKa = 11.84VAEE22 pKa = 4.29STLKK26 pKa = 9.65RR27 pKa = 11.84WRR29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 3.56TRR33 pKa = 11.84KK34 pKa = 8.25TKK36 pKa = 10.71DD37 pKa = 2.85QVGPYY42 pKa = 8.69WVVLSRR48 pKa = 11.84SRR50 pKa = 11.84VGYY53 pKa = 9.66RR54 pKa = 11.84RR55 pKa = 11.84SAIDD59 pKa = 3.12TFLSSRR65 pKa = 11.84EE66 pKa = 3.78QRR68 pKa = 11.84ADD70 pKa = 3.06GG71 pKa = 3.88

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19595

31

1002

192.1

20.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.692 ± 0.419

0.862 ± 0.128

6.053 ± 0.193

7.043 ± 0.347

2.771 ± 0.151

8.655 ± 0.289

1.837 ± 0.162

4.598 ± 0.185

2.853 ± 0.186

7.905 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.116

2.812 ± 0.166

5.797 ± 0.26

3.695 ± 0.369

7.446 ± 0.35

5.588 ± 0.208

6.262 ± 0.245

7.237 ± 0.258

1.929 ± 0.165

2.475 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski