Amino acid dipepetide frequency for Bacillus thermoamylovorans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.94AlaAla: 4.94 ± 0.085	0.572AlaCys: 0.572 ± 0.024	3.149AlaAsp: 3.149 ± 0.05	4.367AlaGlu: 4.367 ± 0.08	3.073AlaPhe: 3.073 ± 0.07	4.953AlaGly: 4.953 ± 0.087	1.104AlaHis: 1.104 ± 0.031	5.974AlaIle: 5.974 ± 0.078	5.135AlaLys: 5.135 ± 0.078	6.41AlaLeu: 6.41 ± 0.087	1.759AlaMet: 1.759 ± 0.047	2.954AlaAsn: 2.954 ± 0.055	1.845AlaPro: 1.845 ± 0.047	1.946AlaGln: 1.946 ± 0.044	2.557AlaArg: 2.557 ± 0.058	3.44AlaSer: 3.44 ± 0.062	3.341AlaThr: 3.341 ± 0.064	5.086AlaVal: 5.086 ± 0.08	0.541AlaTrp: 0.541 ± 0.024	2.164AlaTyr: 2.164 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.39CysAla: 0.39 ± 0.02	0.087CysCys: 0.087 ± 0.01	0.367CysAsp: 0.367 ± 0.019	0.449CysGlu: 0.449 ± 0.024	0.325CysPhe: 0.325 ± 0.02	0.64CysGly: 0.64 ± 0.027	0.192CysHis: 0.192 ± 0.014	0.584CysIle: 0.584 ± 0.024	0.401CysLys: 0.401 ± 0.02	0.697CysLeu: 0.697 ± 0.029	0.159CysMet: 0.159 ± 0.014	0.281CysAsn: 0.281 ± 0.018	0.353CysPro: 0.353 ± 0.02	0.24CysGln: 0.24 ± 0.016	0.279CysArg: 0.279 ± 0.016	0.475CysSer: 0.475 ± 0.023	0.4CysThr: 0.4 ± 0.02	0.386CysVal: 0.386 ± 0.02	0.063CysTrp: 0.063 ± 0.008	0.269CysTyr: 0.269 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	2.941AspAla: 2.941 ± 0.068	0.409AspCys: 0.409 ± 0.022	2.501AspAsp: 2.501 ± 0.065	4.564AspGlu: 4.564 ± 0.074	2.593AspPhe: 2.593 ± 0.052	3.36AspGly: 3.36 ± 0.065	1.128AspHis: 1.128 ± 0.036	4.191AspIle: 4.191 ± 0.074	3.2AspLys: 3.2 ± 0.062	5.041AspLeu: 5.041 ± 0.081	1.211AspMet: 1.211 ± 0.035	1.821AspAsn: 1.821 ± 0.055	2.004AspPro: 2.004 ± 0.052	2.12AspGln: 2.12 ± 0.049	2.162AspArg: 2.162 ± 0.047	2.415AspSer: 2.415 ± 0.058	2.329AspThr: 2.329 ± 0.058	3.538AspVal: 3.538 ± 0.059	0.593AspTrp: 0.593 ± 0.026	2.174AspTyr: 2.174 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.804GluAla: 4.804 ± 0.082	0.363GluCys: 0.363 ± 0.019	3.414GluAsp: 3.414 ± 0.065	6.696GluGlu: 6.696 ± 0.112	2.676GluPhe: 2.676 ± 0.057	3.948GluGly: 3.948 ± 0.059	1.46GluHis: 1.46 ± 0.041	6.344GluIle: 6.344 ± 0.082	7.694GluLys: 7.694 ± 0.117	6.947GluLeu: 6.947 ± 0.087	2.296GluMet: 2.296 ± 0.049	4.146GluAsn: 4.146 ± 0.072	1.993GluPro: 1.993 ± 0.045	3.427GluGln: 3.427 ± 0.066	3.411GluArg: 3.411 ± 0.071	3.265GluSer: 3.265 ± 0.064	3.937GluThr: 3.937 ± 0.072	4.838GluVal: 4.838 ± 0.079	0.754GluTrp: 0.754 ± 0.029	2.335GluTyr: 2.335 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.072PheAla: 3.072 ± 0.055	0.375PheCys: 0.375 ± 0.02	2.384PheAsp: 2.384 ± 0.05	2.831PheGlu: 2.831 ± 0.056	2.645PhePhe: 2.645 ± 0.075	3.429PheGly: 3.429 ± 0.065	1.138PheHis: 1.138 ± 0.04	4.486PheIle: 4.486 ± 0.087	2.538PheLys: 2.538 ± 0.054	5.013PheLeu: 5.013 ± 0.092	1.153PheMet: 1.153 ± 0.032	1.95PheAsn: 1.95 ± 0.04	1.781PhePro: 1.781 ± 0.048	1.843PheGln: 1.843 ± 0.043	1.647PheArg: 1.647 ± 0.04	3.363PheSer: 3.363 ± 0.072	2.712PheThr: 2.712 ± 0.057	3.097PheVal: 3.097 ± 0.062	0.539PheTrp: 0.539 ± 0.031	1.919PheTyr: 1.919 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.34GlyAla: 4.34 ± 0.086	0.578GlyCys: 0.578 ± 0.026	3.079GlyAsp: 3.079 ± 0.065	4.492GlyGlu: 4.492 ± 0.066	3.424GlyPhe: 3.424 ± 0.06	4.712GlyGly: 4.712 ± 0.093	1.297GlyHis: 1.297 ± 0.037	6.247GlyIle: 6.247 ± 0.1	5.53GlyLys: 5.53 ± 0.076	6.249GlyLeu: 6.249 ± 0.096	2.047GlyMet: 2.047 ± 0.05	2.91GlyAsn: 2.91 ± 0.06	1.693GlyPro: 1.693 ± 0.048	2.19GlyGln: 2.19 ± 0.053	2.599GlyArg: 2.599 ± 0.054	3.635GlySer: 3.635 ± 0.067	3.936GlyThr: 3.936 ± 0.066	4.843GlyVal: 4.843 ± 0.074	0.716GlyTrp: 0.716 ± 0.031	2.779GlyTyr: 2.779 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.224HisAla: 1.224 ± 0.034	0.176HisCys: 0.176 ± 0.013	0.952HisAsp: 0.952 ± 0.03	1.329HisGlu: 1.329 ± 0.032	1.125HisPhe: 1.125 ± 0.035	1.348HisGly: 1.348 ± 0.04	0.666HisHis: 0.666 ± 0.029	1.58HisIle: 1.58 ± 0.043	1.031HisLys: 1.031 ± 0.036	2.123HisLeu: 2.123 ± 0.044	0.477HisMet: 0.477 ± 0.022	0.779HisAsn: 0.779 ± 0.029	1.157HisPro: 1.157 ± 0.035	0.746HisGln: 0.746 ± 0.028	0.82HisArg: 0.82 ± 0.029	1.082HisSer: 1.082 ± 0.034	0.99HisThr: 0.99 ± 0.032	1.437HisVal: 1.437 ± 0.038	0.228HisTrp: 0.228 ± 0.015	0.894HisTyr: 0.894 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.949IleAla: 5.949 ± 0.084	0.688IleCys: 0.688 ± 0.029	4.827IleAsp: 4.827 ± 0.082	6.12IleGlu: 6.12 ± 0.087	4.06IlePhe: 4.06 ± 0.086	6.303IleGly: 6.303 ± 0.1	1.831IleHis: 1.831 ± 0.044	7.272IleIle: 7.272 ± 0.108	5.16IleLys: 5.16 ± 0.081	7.989IleLeu: 7.989 ± 0.107	1.929IleMet: 1.929 ± 0.049	3.844IleAsn: 3.844 ± 0.058	3.746IlePro: 3.746 ± 0.063	3.144IleGln: 3.144 ± 0.058	3.416IleArg: 3.416 ± 0.057	5.294IleSer: 5.294 ± 0.08	4.584IleThr: 4.584 ± 0.062	5.914IleVal: 5.914 ± 0.088	0.726IleTrp: 0.726 ± 0.031	2.832IleTyr: 2.832 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	4.502LysAla: 4.502 ± 0.078	0.328LysCys: 0.328 ± 0.017	3.879LysAsp: 3.879 ± 0.074	7.073LysGlu: 7.073 ± 0.092	2.373LysPhe: 2.373 ± 0.054	4.454LysGly: 4.454 ± 0.074	1.259LysHis: 1.259 ± 0.041	5.968LysIle: 5.968 ± 0.075	6.688LysLys: 6.688 ± 0.091	6.394LysLeu: 6.394 ± 0.087	2.304LysMet: 2.304 ± 0.045	4.235LysAsn: 4.235 ± 0.077	2.317LysPro: 2.317 ± 0.05	3.27LysGln: 3.27 ± 0.067	3.419LysArg: 3.419 ± 0.058	3.543LysSer: 3.543 ± 0.058	3.878LysThr: 3.878 ± 0.065	4.763LysVal: 4.763 ± 0.077	0.815LysTrp: 0.815 ± 0.03	2.641LysTyr: 2.641 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	7.14LeuAla: 7.14 ± 0.083	0.643LeuCys: 0.643 ± 0.026	4.653LeuAsp: 4.653 ± 0.07	6.556LeuGlu: 6.556 ± 0.085	5.053LeuPhe: 5.053 ± 0.094	6.113LeuGly: 6.113 ± 0.093	1.887LeuHis: 1.887 ± 0.045	7.792LeuIle: 7.792 ± 0.122	7.155LeuLys: 7.155 ± 0.085	10.101LeuLeu: 10.101 ± 0.131	2.319LeuMet: 2.319 ± 0.049	4.586LeuAsn: 4.586 ± 0.065	3.891LeuPro: 3.891 ± 0.067	3.621LeuGln: 3.621 ± 0.072	3.303LeuArg: 3.303 ± 0.065	6.237LeuSer: 6.237 ± 0.076	5.685LeuThr: 5.685 ± 0.079	6.085LeuVal: 6.085 ± 0.079	0.794LeuTrp: 0.794 ± 0.03	3.238LeuTyr: 3.238 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	1.927MetAla: 1.927 ± 0.042	0.136MetCys: 0.136 ± 0.013	1.444MetAsp: 1.444 ± 0.037	2.165MetGlu: 2.165 ± 0.044	1.065MetPhe: 1.065 ± 0.033	1.685MetGly: 1.685 ± 0.037	0.378MetHis: 0.378 ± 0.022	2.237MetIle: 2.237 ± 0.05	2.377MetLys: 2.377 ± 0.056	2.276MetLeu: 2.276 ± 0.047	0.782MetMet: 0.782 ± 0.034	1.653MetAsn: 1.653 ± 0.042	0.873MetPro: 0.873 ± 0.034	0.824MetGln: 0.824 ± 0.031	0.985MetArg: 0.985 ± 0.03	1.503MetSer: 1.503 ± 0.045	1.535MetThr: 1.535 ± 0.037	1.809MetVal: 1.809 ± 0.042	0.169MetTrp: 0.169 ± 0.013	0.726MetTyr: 0.726 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.78AsnAla: 2.78 ± 0.052	0.33AsnCys: 0.33 ± 0.019	2.483AsnAsp: 2.483 ± 0.058	3.851AsnGlu: 3.851 ± 0.069	1.993AsnPhe: 1.993 ± 0.052	3.478AsnGly: 3.478 ± 0.065	1.224AsnHis: 1.224 ± 0.033	3.965AsnIle: 3.965 ± 0.07	3.281AsnLys: 3.281 ± 0.063	4.232AsnLeu: 4.232 ± 0.074	1.246AsnMet: 1.246 ± 0.034	2.293AsnAsn: 2.293 ± 0.069	2.244AsnPro: 2.244 ± 0.05	2.237AsnGln: 2.237 ± 0.051	2.233AsnArg: 2.233 ± 0.048	2.383AsnSer: 2.383 ± 0.055	2.218AsnThr: 2.218 ± 0.047	2.968AsnVal: 2.968 ± 0.061	0.569AsnTrp: 0.569 ± 0.025	1.728AsnTyr: 1.728 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.073ProAla: 2.073 ± 0.053	0.211ProCys: 0.211 ± 0.015	2.133ProAsp: 2.133 ± 0.044	3.031ProGlu: 3.031 ± 0.055	2.098ProPhe: 2.098 ± 0.047	2.319ProGly: 2.319 ± 0.057	0.722ProHis: 0.722 ± 0.028	3.131ProIle: 3.131 ± 0.061	2.314ProLys: 2.314 ± 0.048	3.377ProLeu: 3.377 ± 0.066	0.83ProMet: 0.83 ± 0.037	1.846ProAsn: 1.846 ± 0.039	0.941ProPro: 0.941 ± 0.033	0.996ProGln: 0.996 ± 0.033	1.065ProArg: 1.065 ± 0.034	2.11ProSer: 2.11 ± 0.043	1.965ProThr: 1.965 ± 0.039	2.924ProVal: 2.924 ± 0.056	0.338ProTrp: 0.338 ± 0.021	1.467ProTyr: 1.467 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.586GlnAla: 2.586 ± 0.056	0.179GlnCys: 0.179 ± 0.013	1.562GlnAsp: 1.562 ± 0.042	2.813GlnGlu: 2.813 ± 0.056	1.815GlnPhe: 1.815 ± 0.049	2.028GlnGly: 2.028 ± 0.05	0.63GlnHis: 0.63 ± 0.027	3.144GlnIle: 3.144 ± 0.066	2.925GlnLys: 2.925 ± 0.06	4.205GlnLeu: 4.205 ± 0.081	1.173GlnMet: 1.173 ± 0.036	1.895GlnAsn: 1.895 ± 0.048	1.139GlnPro: 1.139 ± 0.043	1.544GlnGln: 1.544 ± 0.043	1.275GlnArg: 1.275 ± 0.034	1.972GlnSer: 1.972 ± 0.045	2.059GlnThr: 2.059 ± 0.049	2.371GlnVal: 2.371 ± 0.043	0.38GlnTrp: 0.38 ± 0.02	1.433GlnTyr: 1.433 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.351ArgAla: 2.351 ± 0.045	0.226ArgCys: 0.226 ± 0.016	2.022ArgAsp: 2.022 ± 0.049	3.198ArgGlu: 3.198 ± 0.069	1.941ArgPhe: 1.941 ± 0.048	2.297ArgGly: 2.297 ± 0.051	0.749ArgHis: 0.749 ± 0.024	3.13ArgIle: 3.13 ± 0.065	3.51ArgLys: 3.51 ± 0.059	3.955ArgLeu: 3.955 ± 0.076	1.212ArgMet: 1.212 ± 0.04	2.075ArgAsn: 2.075 ± 0.046	1.308ArgPro: 1.308 ± 0.039	1.534ArgGln: 1.534 ± 0.042	1.791ArgArg: 1.791 ± 0.055	1.958ArgSer: 1.958 ± 0.047	2.094ArgThr: 2.094 ± 0.045	2.513ArgVal: 2.513 ± 0.056	0.379ArgTrp: 0.379 ± 0.02	1.459ArgTyr: 1.459 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.253SerAla: 3.253 ± 0.057	0.43SerCys: 0.43 ± 0.021	2.608SerAsp: 2.608 ± 0.055	3.549SerGlu: 3.549 ± 0.058	3.157SerPhe: 3.157 ± 0.065	4.322SerGly: 4.322 ± 0.072	1.038SerHis: 1.038 ± 0.031	5.116SerIle: 5.116 ± 0.08	3.584SerLys: 3.584 ± 0.054	5.782SerLeu: 5.782 ± 0.069	1.509SerMet: 1.509 ± 0.037	2.487SerAsn: 2.487 ± 0.049	2.03SerPro: 2.03 ± 0.042	1.847SerGln: 1.847 ± 0.047	2.192SerArg: 2.192 ± 0.049	3.357SerSer: 3.357 ± 0.064	2.949SerThr: 2.949 ± 0.05	3.858SerVal: 3.858 ± 0.067	0.578SerTrp: 0.578 ± 0.024	2.016SerTyr: 2.016 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	3.73ThrAla: 3.73 ± 0.069	0.374ThrCys: 0.374 ± 0.02	2.828ThrAsp: 2.828 ± 0.056	3.555ThrGlu: 3.555 ± 0.059	2.688ThrPhe: 2.688 ± 0.054	4.352ThrGly: 4.352 ± 0.073	0.975ThrHis: 0.975 ± 0.031	4.828ThrIle: 4.828 ± 0.075	3.592ThrLys: 3.592 ± 0.051	4.835ThrLeu: 4.835 ± 0.071	1.33ThrMet: 1.33 ± 0.037	2.732ThrAsn: 2.732 ± 0.057	2.154ThrPro: 2.154 ± 0.049	1.265ThrGln: 1.265 ± 0.035	1.87ThrArg: 1.87 ± 0.05	2.971ThrSer: 2.971 ± 0.055	2.83ThrThr: 2.83 ± 0.057	4.286ThrVal: 4.286 ± 0.071	0.492ThrTrp: 0.492 ± 0.023	1.943ThrTyr: 1.943 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.719ValAla: 4.719 ± 0.077	0.543ValCys: 0.543 ± 0.022	3.631ValAsp: 3.631 ± 0.056	4.793ValGlu: 4.793 ± 0.079	3.216ValPhe: 3.216 ± 0.06	4.461ValGly: 4.461 ± 0.068	1.348ValHis: 1.348 ± 0.042	6.041ValIle: 6.041 ± 0.075	5.048ValLys: 5.048 ± 0.074	6.458ValLeu: 6.458 ± 0.076	1.692ValMet: 1.692 ± 0.041	3.238ValAsn: 3.238 ± 0.058	2.661ValPro: 2.661 ± 0.049	2.321ValGln: 2.321 ± 0.054	2.553ValArg: 2.553 ± 0.057	4.175ValSer: 4.175 ± 0.069	3.849ValThr: 3.849 ± 0.067	4.847ValVal: 4.847 ± 0.076	0.606ValTrp: 0.606 ± 0.026	2.236ValTyr: 2.236 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.565TrpAla: 0.565 ± 0.023	0.073TrpCys: 0.073 ± 0.009	0.462TrpAsp: 0.462 ± 0.024	0.67TrpGlu: 0.67 ± 0.027	0.545TrpPhe: 0.545 ± 0.028	0.597TrpGly: 0.597 ± 0.027	0.199TrpHis: 0.199 ± 0.014	0.821TrpIle: 0.821 ± 0.03	0.713TrpLys: 0.713 ± 0.025	1.12TrpLeu: 1.12 ± 0.04	0.29TrpMet: 0.29 ± 0.019	0.514TrpAsn: 0.514 ± 0.024	0.279TrpPro: 0.279 ± 0.018	0.402TrpGln: 0.402 ± 0.021	0.4TrpArg: 0.4 ± 0.023	0.52TrpSer: 0.52 ± 0.025	0.527TrpThr: 0.527 ± 0.027	0.631TrpVal: 0.631 ± 0.031	0.127TrpTrp: 0.127 ± 0.013	0.342TrpTyr: 0.342 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.025TyrAla: 2.025 ± 0.05	0.314TyrCys: 0.314 ± 0.018	1.914TyrAsp: 1.914 ± 0.045	2.645TyrGlu: 2.645 ± 0.053	2.05TyrPhe: 2.05 ± 0.044	2.497TyrGly: 2.497 ± 0.053	0.939TyrHis: 0.939 ± 0.031	2.779TyrIle: 2.779 ± 0.055	2.227TyrLys: 2.227 ± 0.047	3.647TyrLeu: 3.647 ± 0.061	0.83TyrMet: 0.83 ± 0.032	1.496TyrAsn: 1.496 ± 0.037	1.483TyrPro: 1.483 ± 0.038	1.612TyrGln: 1.612 ± 0.036	1.729TyrArg: 1.729 ± 0.043	1.963TyrSer: 1.963 ± 0.046	1.813TyrThr: 1.813 ± 0.042	2.243TyrVal: 2.243 ± 0.046	0.388TyrTrp: 0.388 ± 0.02	1.536TyrTyr: 1.536 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3440 proteins (983123 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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