Valsa mali var. pyri

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Diaporthales; Valsaceae; Valsa; Valsa mali

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10741 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A194UQF3|A0A194UQF3_9PEZI Uncharacterized protein OS=Valsa mali var. pyri OX=694573 GN=VP1G_01238 PE=4 SV=1
MM1 pKa = 7.59HH2 pKa = 7.11FRR4 pKa = 11.84SPIWAAGTALLLASSATAQTSTSCNPTEE32 pKa = 4.45TTCDD36 pKa = 3.61PDD38 pKa = 3.39EE39 pKa = 4.53ALGMAIDD46 pKa = 3.62VDD48 pKa = 4.73FTQGEE53 pKa = 4.69VNSFTSSGGTPSYY66 pKa = 11.14GSDD69 pKa = 2.71GVTFTISKK77 pKa = 10.5EE78 pKa = 3.65GDD80 pKa = 3.15APQLNSIFYY89 pKa = 10.0IMFGKK94 pKa = 10.51VEE96 pKa = 4.09ITMKK100 pKa = 10.22CAPGTGIVTSVVLQSDD116 pKa = 4.11DD117 pKa = 3.97LDD119 pKa = 4.73EE120 pKa = 6.67IDD122 pKa = 5.02MEE124 pKa = 5.05FLGTDD129 pKa = 3.44DD130 pKa = 5.82SNVQLMYY137 pKa = 10.46FGKK140 pKa = 10.7GEE142 pKa = 3.97RR143 pKa = 11.84ADD145 pKa = 3.72NAVVNADD152 pKa = 3.9APNSQEE158 pKa = 4.86DD159 pKa = 4.28FVTYY163 pKa = 9.59TVDD166 pKa = 2.81WSSEE170 pKa = 3.95RR171 pKa = 11.84IAWSVAGTVVRR182 pKa = 11.84VLEE185 pKa = 4.29STTYY189 pKa = 10.02PDD191 pKa = 4.76YY192 pKa = 11.32YY193 pKa = 9.89PQTPMQLKK201 pKa = 9.38FGIWAGGDD209 pKa = 3.51PKK211 pKa = 11.01NSEE214 pKa = 4.35GTISWAGGTTDD225 pKa = 3.94YY226 pKa = 11.66SDD228 pKa = 4.4GPFSATVQRR237 pKa = 11.84VAVTDD242 pKa = 3.67YY243 pKa = 10.55STGSSYY249 pKa = 11.05TYY251 pKa = 11.11GNTSGLWEE259 pKa = 4.8SIEE262 pKa = 4.18SDD264 pKa = 3.04GGEE267 pKa = 3.86INANADD273 pKa = 3.27EE274 pKa = 4.62AATVTSVSGATSTGSSDD291 pKa = 3.72FPGSGIGDD299 pKa = 3.61GTSTVTASIEE309 pKa = 4.26SMPSGWHH316 pKa = 4.62MTSSGKK322 pKa = 9.67IVPNGSSAINPPHH335 pKa = 6.83ILLLAGPLSFFIFVVCNGGWRR356 pKa = 3.41

Molecular weight: 37.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A194ULZ4|A0A194ULZ4_9PEZI TPT domain-containing protein OS=Valsa mali var. pyri OX=694573 GN=VP1G_00193 PE=4 SV=1
MM1 pKa = 7.41SHH3 pKa = 6.25EE4 pKa = 4.71SVWNSRR10 pKa = 11.84PRR12 pKa = 11.84QYY14 pKa = 11.58GKK16 pKa = 10.29GARR19 pKa = 11.84EE20 pKa = 3.99CRR22 pKa = 11.84VCTHH26 pKa = 6.93RR27 pKa = 11.84AGLIRR32 pKa = 11.84KK33 pKa = 8.91YY34 pKa = 10.76GLNICRR40 pKa = 11.84QCFRR44 pKa = 11.84EE45 pKa = 4.01KK46 pKa = 10.63SADD49 pKa = 3.07IGFVKK54 pKa = 10.35HH55 pKa = 5.95RR56 pKa = 4.18

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10741

0

10741

5305359

50

5615

493.9

54.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.799 ± 0.02

1.145 ± 0.007

5.928 ± 0.015

6.27 ± 0.026

3.593 ± 0.013

7.251 ± 0.023

2.334 ± 0.01

4.708 ± 0.015

4.83 ± 0.022

8.651 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.009

3.636 ± 0.011

6.097 ± 0.026

3.954 ± 0.014

5.982 ± 0.021

8.048 ± 0.027

6.031 ± 0.021

6.281 ± 0.017

1.463 ± 0.008

2.739 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski