Xingshan nematode virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKI9|A0A1L3KKI9_9VIRU DiSB-ORF2_chro domain-containing protein OS=Xingshan nematode virus 2 OX=1923761 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84VCVLLISFFGAFLGSTEE19 pKa = 4.12RR20 pKa = 11.84FGLRR24 pKa = 11.84YY25 pKa = 9.53HH26 pKa = 6.07EE27 pKa = 4.71RR28 pKa = 11.84ARR30 pKa = 11.84KK31 pKa = 9.3IPGAYY36 pKa = 9.18DD37 pKa = 3.3VYY39 pKa = 11.1LDD41 pKa = 3.93SLISKK46 pKa = 9.69YY47 pKa = 10.87EE48 pKa = 3.76NASYY52 pKa = 10.39YY53 pKa = 10.88LKK55 pKa = 10.61IFGLVTSNQAKK66 pKa = 10.18RR67 pKa = 11.84LIEE70 pKa = 4.2EE71 pKa = 4.38LDD73 pKa = 3.68SLRR76 pKa = 11.84KK77 pKa = 9.5EE78 pKa = 3.76IQDD81 pKa = 3.28RR82 pKa = 11.84VDD84 pKa = 3.56YY85 pKa = 11.15VSEE88 pKa = 3.77NHH90 pKa = 6.22KK91 pKa = 10.25RR92 pKa = 11.84MSMIPFGVLANRR104 pKa = 11.84YY105 pKa = 8.61IVDD108 pKa = 3.8DD109 pKa = 4.01AVVAGFRR116 pKa = 11.84SIDD119 pKa = 3.39YY120 pKa = 9.93YY121 pKa = 11.27RR122 pKa = 11.84RR123 pKa = 11.84ILSFCLADD131 pKa = 3.62LEE133 pKa = 4.84SRR135 pKa = 11.84ILTVSEE141 pKa = 4.22TFLDD145 pKa = 4.45PVCMSAYY152 pKa = 9.74NKK154 pKa = 10.39SFNEE158 pKa = 5.03DD159 pKa = 2.66IDD161 pKa = 4.25CRR163 pKa = 11.84LSSLCFGVIKK173 pKa = 10.79KK174 pKa = 10.15NVDD177 pKa = 3.22GLFICSGYY185 pKa = 10.59SKK187 pKa = 11.12NLFRR191 pKa = 11.84HH192 pKa = 4.78YY193 pKa = 10.62RR194 pKa = 11.84LAKK197 pKa = 8.88STLLADD203 pKa = 3.72VFPLRR208 pKa = 11.84EE209 pKa = 4.15GQFEE213 pKa = 4.45FYY215 pKa = 10.97CNVSNNRR222 pKa = 11.84PLLVHH227 pKa = 6.98KK228 pKa = 10.75SLLNGDD234 pKa = 3.92KK235 pKa = 10.82RR236 pKa = 11.84FVDD239 pKa = 3.94PFSVLLQSDD248 pKa = 4.52FPFLTFSDD256 pKa = 3.33GGYY259 pKa = 9.28RR260 pKa = 11.84ALVFSQFVDD269 pKa = 3.55SYY271 pKa = 10.01STVSLATAGFKK282 pKa = 9.59YY283 pKa = 10.5VCNDD287 pKa = 3.18TLFAEE292 pKa = 4.7SSIIDD297 pKa = 3.76DD298 pKa = 3.73VNVFRR303 pKa = 11.84SFRR306 pKa = 11.84GCRR309 pKa = 11.84NFVLTEE315 pKa = 4.13GSDD318 pKa = 3.64VSSKK322 pKa = 10.43ICVDD326 pKa = 3.37HH327 pKa = 7.35LYY329 pKa = 10.79QDD331 pKa = 4.09FQALVDD337 pKa = 4.1RR338 pKa = 11.84CPEE341 pKa = 3.42DD342 pKa = 3.54WYY344 pKa = 10.17TFDD347 pKa = 3.85HH348 pKa = 6.77AVATFEE354 pKa = 4.61TYY356 pKa = 10.13TIGINDD362 pKa = 3.74YY363 pKa = 11.54SEE365 pKa = 5.04VLWKK369 pKa = 9.08TIPQVVTDD377 pKa = 4.01LLRR380 pKa = 11.84YY381 pKa = 9.52FDD383 pKa = 4.4RR384 pKa = 11.84NHH386 pKa = 6.1INVRR390 pKa = 11.84VQDD393 pKa = 3.62FEE395 pKa = 5.12KK396 pKa = 10.41FYY398 pKa = 11.22KK399 pKa = 10.86DD400 pKa = 3.06FVDD403 pKa = 3.89YY404 pKa = 10.98VRR406 pKa = 11.84SFGTLTNVTVRR417 pKa = 11.84FADD420 pKa = 3.44QVFDD424 pKa = 4.9GIGANLRR431 pKa = 11.84LLDD434 pKa = 3.84SFTFRR439 pKa = 11.84NFSEE443 pKa = 4.43NFVDD447 pKa = 4.45YY448 pKa = 10.02FWAKK452 pKa = 10.84AMGVDD457 pKa = 3.81VEE459 pKa = 4.38QIRR462 pKa = 11.84DD463 pKa = 3.65TTHH466 pKa = 6.44FEE468 pKa = 4.48SIHH471 pKa = 6.15SVTSVSGWLAQAISSFVRR489 pKa = 11.84PFWQVFLEE497 pKa = 4.01LLEE500 pKa = 5.64DD501 pKa = 3.45ILDD504 pKa = 3.96IIIRR508 pKa = 11.84VLFDD512 pKa = 3.52LTPLLEE518 pKa = 4.09KK519 pKa = 10.33FYY521 pKa = 10.85RR522 pKa = 11.84LTLAAFDD529 pKa = 4.19SLLNLLFQVLTLVLSFLLHH548 pKa = 6.4ILIILEE554 pKa = 4.23SKK556 pKa = 10.47ILLSEE561 pKa = 4.09YY562 pKa = 10.65LLVYY566 pKa = 10.4LFLSRR571 pKa = 11.84YY572 pKa = 7.19WSSPVPPLLILLLLILVFGFQRR594 pKa = 11.84SYY596 pKa = 11.05PSFLFFVLNHH606 pKa = 5.95QIKK609 pKa = 10.39SVLNATAIPSVTYY622 pKa = 10.14DD623 pKa = 3.17YY624 pKa = 11.5QFVYY628 pKa = 10.9NITLVNYY635 pKa = 7.34THH637 pKa = 6.99HH638 pKa = 7.42LYY640 pKa = 10.67HH641 pKa = 6.64FSIVNTPYY649 pKa = 10.21SYY651 pKa = 11.36SFYY654 pKa = 10.58VSNVSVFTYY663 pKa = 10.39VSEE666 pKa = 4.3KK667 pKa = 10.37FSSFNSTFFVTTFSHH682 pKa = 6.48YY683 pKa = 10.7LDD685 pKa = 4.86SSDD688 pKa = 5.38DD689 pKa = 3.72CDD691 pKa = 3.95FVCFLKK697 pKa = 11.0FFGFF701 pKa = 4.04

Molecular weight: 81.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KKI9|A0A1L3KKI9_9VIRU DiSB-ORF2_chro domain-containing protein OS=Xingshan nematode virus 2 OX=1923761 PE=4 SV=1
MM1 pKa = 7.54ASSALMLPSEE11 pKa = 4.49NGRR14 pKa = 11.84PPKK17 pKa = 10.36FYY19 pKa = 11.19DD20 pKa = 3.26HH21 pKa = 6.94ATGIQTVRR29 pKa = 11.84GGQQVGEE36 pKa = 4.45GFFSSVFNSFYY47 pKa = 10.86NIIYY51 pKa = 9.0HH52 pKa = 6.15FWGLTFAVLGIFTLLSEE69 pKa = 4.67YY70 pKa = 9.52GTASGPLEE78 pKa = 4.12VLLQAILKK86 pKa = 8.92FISDD90 pKa = 3.93PDD92 pKa = 3.62VPIVLKK98 pKa = 10.96SIASAFAWLLGYY110 pKa = 7.75MVKK113 pKa = 10.52YY114 pKa = 9.75KK115 pKa = 10.98YY116 pKa = 10.48VVAYY120 pKa = 9.9SAILFVPAIVKK131 pKa = 10.03PSTRR135 pKa = 11.84NIMFSFLLIFMAFLHH150 pKa = 6.66YY151 pKa = 9.58ITILQVLLLSLLFYY165 pKa = 10.45LFVMLRR171 pKa = 11.84TPAHH175 pKa = 6.14KK176 pKa = 10.4FFVLFMALIVFSVGFTEE193 pKa = 4.27HH194 pKa = 6.76QSFLSSKK201 pKa = 10.91LNLLLPADD209 pKa = 4.21LSKK212 pKa = 11.02PLTFKK217 pKa = 10.94PNTFLLPDD225 pKa = 3.9AQSPSARR232 pKa = 11.84AKK234 pKa = 10.1RR235 pKa = 11.84DD236 pKa = 3.2EE237 pKa = 4.1NEE239 pKa = 3.88ILRR242 pKa = 11.84HH243 pKa = 5.09QVAQLMEE250 pKa = 5.47KK251 pKa = 9.74ISNMHH256 pKa = 6.03QEE258 pKa = 4.26MEE260 pKa = 4.38TMRR263 pKa = 11.84QAASKK268 pKa = 10.13PNEE271 pKa = 4.11ATTAAPTVLPRR282 pKa = 11.84TEE284 pKa = 3.84RR285 pKa = 11.84HH286 pKa = 5.66KK287 pKa = 11.28SSS289 pKa = 3.12

Molecular weight: 32.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3712

289

2722

1237.3

142.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.55 ± 0.783

2.182 ± 0.574

7.462 ± 1.359

5.038 ± 0.559

6.897 ± 1.484

4.31 ± 0.301

1.913 ± 0.246

5.711 ± 0.06

6.008 ± 1.113

9.833 ± 1.511

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.586 ± 0.696

4.095 ± 0.225

2.963 ± 0.67

2.344 ± 0.362

5.792 ± 0.618

7.678 ± 0.926

4.984 ± 0.134

8.944 ± 0.546

0.7 ± 0.06

5.011 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski