Candidatus Gallionella acididurans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Gallionellaceae; Gallionella

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139BNH7|A0A139BNH7_9PROT Uncharacterized protein OS=Candidatus Gallionella acididurans OX=1796491 GN=AWT59_3357 PE=4 SV=1
MM1 pKa = 7.56SYY3 pKa = 8.58VTKK6 pKa = 10.51ICIALFALLLSGQNASASMDD26 pKa = 3.71CEE28 pKa = 4.16GTFPNFITDD37 pKa = 3.78VCWSCAFPIEE47 pKa = 4.78FGTVPINITGSSGQEE62 pKa = 3.69TTVDD66 pKa = 3.44SGVGAVCICGINPGVTISFWEE87 pKa = 4.16PLRR90 pKa = 11.84DD91 pKa = 2.99IDD93 pKa = 4.45VVRR96 pKa = 11.84KK97 pKa = 7.25PFCMSTLGGVDD108 pKa = 3.6MNPGFDD114 pKa = 4.19APHH117 pKa = 6.02GTQTKK122 pKa = 10.16KK123 pKa = 10.85DD124 pKa = 3.86NSDD127 pKa = 3.09MTSFYY132 pKa = 9.79QAHH135 pKa = 6.88WYY137 pKa = 9.83VDD139 pKa = 3.41PLMQLLQLVLDD150 pKa = 4.28SRR152 pKa = 11.84CIEE155 pKa = 3.85QKK157 pKa = 10.93GFDD160 pKa = 3.5VAYY163 pKa = 9.04LTEE166 pKa = 5.83FDD168 pKa = 4.94PLWNDD173 pKa = 4.06DD174 pKa = 3.83EE175 pKa = 4.41MTAIINPDD183 pKa = 2.89SFLFGNLPAQQ193 pKa = 3.69

Molecular weight: 21.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139BQP0|A0A139BQP0_9PROT Uncharacterized protein OS=Candidatus Gallionella acididurans OX=1796491 GN=AWT59_2558 PE=4 SV=1
MM1 pKa = 7.75GLRR4 pKa = 11.84SCGIANLKK12 pKa = 9.78IRR14 pKa = 11.84DD15 pKa = 3.49IDD17 pKa = 3.47WRR19 pKa = 11.84VGIVTLRR26 pKa = 11.84GTKK29 pKa = 8.37SLRR32 pKa = 11.84QDD34 pKa = 3.48VLPLPMEE41 pKa = 4.78TGQALVDD48 pKa = 3.49YY49 pKa = 9.05LQNEE53 pKa = 4.75RR54 pKa = 11.84PRR56 pKa = 11.84TANPSVFVPRR66 pKa = 11.84LGDD69 pKa = 3.17HH70 pKa = 7.08DD71 pKa = 4.93RR72 pKa = 11.84PMTAGAVRR80 pKa = 11.84GVIRR84 pKa = 11.84WAYY87 pKa = 8.93KK88 pKa = 10.36RR89 pKa = 11.84IGLTHH94 pKa = 6.08TRR96 pKa = 11.84SHH98 pKa = 6.95ALRR101 pKa = 11.84HH102 pKa = 5.54AFACRR107 pKa = 11.84LVEE110 pKa = 4.42SGSSLKK116 pKa = 10.49EE117 pKa = 3.69VADD120 pKa = 4.16LLRR123 pKa = 11.84HH124 pKa = 6.13RR125 pKa = 11.84SLSTTLIYY133 pKa = 10.91AKK135 pKa = 10.61LDD137 pKa = 3.76TPKK140 pKa = 10.35LAAIALPWPGSTSS153 pKa = 3.03

Molecular weight: 16.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3512

0

3512

1004978

18

1965

286.2

31.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.555 ± 0.048

0.981 ± 0.016

5.183 ± 0.032

5.596 ± 0.045

3.805 ± 0.032

7.632 ± 0.041

2.427 ± 0.023

5.744 ± 0.032

4.499 ± 0.038

10.638 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.023

3.579 ± 0.032

4.543 ± 0.029

4.105 ± 0.033

5.923 ± 0.033

5.878 ± 0.033

5.128 ± 0.034

7.06 ± 0.043

1.279 ± 0.017

2.68 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski