Bacillus sp. cl95

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3914 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6B4J0|A0A1I6B4J0_9BACI 1 4-alpha-glucan branching enzyme GlgB OS=Bacillus sp. cl95 OX=1761761 GN=glgB PE=3 SV=1
MM1 pKa = 7.12YY2 pKa = 10.44NGYY5 pKa = 10.19EE6 pKa = 4.0NEE8 pKa = 4.04DD9 pKa = 3.66DD10 pKa = 4.1YY11 pKa = 12.24VRR13 pKa = 11.84SLKK16 pKa = 11.08KK17 pKa = 9.97NDD19 pKa = 3.33TYY21 pKa = 11.15RR22 pKa = 11.84FSYY25 pKa = 10.39NYY27 pKa = 9.4EE28 pKa = 3.57IVVNRR33 pKa = 11.84FGDD36 pKa = 3.67GDD38 pKa = 4.62DD39 pKa = 4.64DD40 pKa = 4.92VEE42 pKa = 4.25LANATVDD49 pKa = 3.56ITVSWDD55 pKa = 3.28DD56 pKa = 3.67SSVPGYY62 pKa = 10.25IISWNVNAPTSLPNEE77 pKa = 4.03WTNSEE82 pKa = 4.11EE83 pKa = 4.31EE84 pKa = 4.06IVKK87 pKa = 10.01EE88 pKa = 4.07VIVMYY93 pKa = 10.36LYY95 pKa = 11.19SDD97 pKa = 4.48LEE99 pKa = 4.41ANGISSEE106 pKa = 4.33TFKK109 pKa = 11.08FVV111 pKa = 3.09

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6C972|A0A1I6C972_9BACI Two-component system response regulator YesN OS=Bacillus sp. cl95 OX=1761761 GN=SAMN04488577_3534 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.47VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.77VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3914

0

3914

1110460

25

2468

283.7

31.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.781 ± 0.039

0.705 ± 0.012

5.067 ± 0.036

7.535 ± 0.055

4.762 ± 0.039

6.9 ± 0.04

2.07 ± 0.019

7.805 ± 0.041

7.282 ± 0.038

9.844 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.017

4.495 ± 0.028

3.501 ± 0.023

3.522 ± 0.028

3.922 ± 0.027

6.093 ± 0.03

5.304 ± 0.031

6.987 ± 0.035

1.044 ± 0.016

3.484 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski