Bacillus phage GA-1 (Bacteriophage GA-1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Tatarstanvirinae; Gaunavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9FZV1|Q9FZV1_BPGA1 Uncharacterized protein OS=Bacillus phage GA-1 OX=12345 PE=4 SV=1
MM1 pKa = 7.14ITMTDD6 pKa = 4.5FISLVEE12 pKa = 4.02EE13 pKa = 3.87NMIYY17 pKa = 10.88ALFEE21 pKa = 4.14LSQPSEE27 pKa = 4.28GIEE30 pKa = 3.89QVIVHH35 pKa = 5.83YY36 pKa = 10.46KK37 pKa = 10.49DD38 pKa = 4.1YY39 pKa = 10.59EE40 pKa = 4.24IPNSHH45 pKa = 6.56YY46 pKa = 11.17YY47 pKa = 10.26KK48 pKa = 10.36IQAQDD53 pKa = 2.62IYY55 pKa = 11.6GEE57 pKa = 4.13LLVTLEE63 pKa = 4.43IFSDD67 pKa = 4.33YY68 pKa = 10.27IDD70 pKa = 4.49RR71 pKa = 11.84PIEE74 pKa = 3.95TVMTSSLEE82 pKa = 3.87EE83 pKa = 3.8TFNIMKK89 pKa = 10.18EE90 pKa = 4.12HH91 pKa = 7.73DD92 pKa = 3.55DD93 pKa = 4.44KK94 pKa = 12.04AWFDD98 pKa = 4.0KK99 pKa = 10.85KK100 pKa = 8.37WW101 pKa = 3.04

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9FZV3|Q9FZV3_BPGA1 Uncharacterized protein OS=Bacillus phage GA-1 OX=12345 PE=4 SV=1
MM1 pKa = 7.35FATTATVIGIFFIGLLVGHH20 pKa = 6.78TLTKK24 pKa = 10.39KK25 pKa = 10.54

Molecular weight: 2.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

6563

25

740

187.5

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.906 ± 0.373

0.762 ± 0.172

6.552 ± 0.287

7.649 ± 0.56

5.043 ± 0.42

6.019 ± 0.428

1.783 ± 0.18

7.055 ± 0.434

8.152 ± 0.701

8.167 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.188

6.339 ± 0.381

2.712 ± 0.32

3.398 ± 0.273

3.977 ± 0.289

5.592 ± 0.314

6.171 ± 0.331

6.704 ± 0.35

1.204 ± 0.215

4.906 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski