Flavobacterium noncentrifugens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8VCF8|A0A1G8VCF8_9FLAO Aquaporin Z OS=Flavobacterium noncentrifugens OX=1128970 GN=SAMN04487935_1286 PE=3 SV=1
MM1 pKa = 7.54EE2 pKa = 5.29NNYY5 pKa = 9.84LSKK8 pKa = 10.48IISFCPKK15 pKa = 10.41AILLGLLAFSPKK27 pKa = 10.35GIAQSYY33 pKa = 7.12TVTPIPFQVYY43 pKa = 8.05TADD46 pKa = 3.62TQVQGTADD54 pKa = 3.82DD55 pKa = 4.86LYY57 pKa = 11.37SPVITLPFNFTFYY70 pKa = 11.24GNSYY74 pKa = 8.37SQIVISTNGYY84 pKa = 7.64IDD86 pKa = 3.77FRR88 pKa = 11.84IGMTGLMSEE97 pKa = 4.13WDD99 pKa = 4.08LNGLTIPSANFATKK113 pKa = 10.7NSILGAYY120 pKa = 9.33HH121 pKa = 7.53DD122 pKa = 5.36MYY124 pKa = 11.36NSSQDD129 pKa = 3.62PNVGSITMGTFGFAPYY145 pKa = 10.23RR146 pKa = 11.84RR147 pKa = 11.84FIVMFDD153 pKa = 3.5NNSHH157 pKa = 5.98YY158 pKa = 10.97SCQAVKK164 pKa = 10.77SSFQMILYY172 pKa = 7.05EE173 pKa = 4.12TTNIIDD179 pKa = 3.4VQLIDD184 pKa = 4.93KK185 pKa = 7.46PICAGWEE192 pKa = 3.47QGAAVTGIINSTGTEE207 pKa = 3.76AVTPPGRR214 pKa = 11.84NTGAWTAFHH223 pKa = 6.48EE224 pKa = 5.09GWRR227 pKa = 11.84FQDD230 pKa = 4.16LEE232 pKa = 4.09SSTAYY237 pKa = 10.85NYY239 pKa = 7.6TKK241 pKa = 10.69CDD243 pKa = 3.49ANTDD247 pKa = 3.17GFEE250 pKa = 4.03IFNLNVAQNDD260 pKa = 3.67LWPANPSGIHH270 pKa = 6.34FYY272 pKa = 10.97SSEE275 pKa = 3.83TDD277 pKa = 3.52AVSEE281 pKa = 4.3TNALSLNYY289 pKa = 10.22TNTTANEE296 pKa = 3.66QTIYY300 pKa = 10.74TNIGGLVKK308 pKa = 10.29QIILRR313 pKa = 11.84TVDD316 pKa = 3.32CANDD320 pKa = 3.58FDD322 pKa = 6.56ADD324 pKa = 4.09TVASADD330 pKa = 3.6EE331 pKa = 4.61DD332 pKa = 4.29LNHH335 pKa = 7.53DD336 pKa = 4.14GNLANDD342 pKa = 3.93DD343 pKa = 3.96TDD345 pKa = 4.88GDD347 pKa = 4.51GIPNFTDD354 pKa = 3.27NDD356 pKa = 4.1DD357 pKa = 5.25DD358 pKa = 5.92GDD360 pKa = 4.12LVLTNVEE367 pKa = 4.35YY368 pKa = 11.14VFPKK372 pKa = 10.37SGNAVLDD379 pKa = 3.83TDD381 pKa = 3.94SDD383 pKa = 4.59GIPDD387 pKa = 4.22YY388 pKa = 11.51LDD390 pKa = 4.92ADD392 pKa = 4.28DD393 pKa = 6.28DD394 pKa = 5.06GDD396 pKa = 4.18GVLTINEE403 pKa = 4.68DD404 pKa = 3.68YY405 pKa = 11.08NGNHH409 pKa = 6.01NPIDD413 pKa = 5.28DD414 pKa = 4.14DD415 pKa = 3.89TDD417 pKa = 3.94NNGTPDD423 pKa = 3.74YY424 pKa = 10.12LQLGVALGTNGFNIAKK440 pKa = 9.78NSIAIFPNPASDD452 pKa = 3.89VLNIEE457 pKa = 4.39NKK459 pKa = 10.07SSEE462 pKa = 4.48AITNVSVYY470 pKa = 10.3SINGVLVKK478 pKa = 9.95EE479 pKa = 5.08AKK481 pKa = 10.16NAMDD485 pKa = 5.16KK486 pKa = 10.85ISVSDD491 pKa = 3.93LQTGVYY497 pKa = 9.05FVKK500 pKa = 10.45IQAGNQTLNYY510 pKa = 10.15KK511 pKa = 10.04FIKK514 pKa = 9.96KK515 pKa = 9.89

Molecular weight: 56.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8S7U0|A0A1G8S7U0_9FLAO Uncharacterized protein OS=Flavobacterium noncentrifugens OX=1128970 GN=SAMN04487935_0440 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.35KK16 pKa = 10.31RR17 pKa = 11.84KK18 pKa = 9.82KK19 pKa = 8.65RR20 pKa = 11.84ARR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.91KK27 pKa = 10.59KK28 pKa = 9.82KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3558

0

3558

1205208

30

3285

338.7

37.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.202 ± 0.052

0.801 ± 0.016

5.374 ± 0.036

5.985 ± 0.053

5.415 ± 0.04

6.501 ± 0.05

1.708 ± 0.021

7.797 ± 0.041

7.47 ± 0.061

8.952 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.022

6.227 ± 0.042

3.57 ± 0.029

3.425 ± 0.021

3.25 ± 0.027

6.653 ± 0.033

6.218 ± 0.081

6.229 ± 0.033

1.023 ± 0.015

3.961 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski