Amino acid dipepetide frequency for Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.614AlaAla: 5.614 ± 0.216
0.466AlaCys: 0.466 ± 0.028
4.005AlaAsp: 4.005 ± 0.1
3.81AlaGlu: 3.81 ± 0.116
2.991AlaPhe: 2.991 ± 0.087
4.919AlaGly: 4.919 ± 0.117
1.321AlaHis: 1.321 ± 0.045
5.716AlaIle: 5.716 ± 0.196
5.687AlaLys: 5.687 ± 0.211
6.719AlaLeu: 6.719 ± 0.137
1.68AlaMet: 1.68 ± 0.067
3.561AlaAsn: 3.561 ± 0.12
2.038AlaPro: 2.038 ± 0.061
2.984AlaGln: 2.984 ± 0.119
2.504AlaArg: 2.504 ± 0.077
4.085AlaSer: 4.085 ± 0.101
4.227AlaThr: 4.227 ± 0.144
4.8AlaVal: 4.8 ± 0.114
0.657AlaTrp: 0.657 ± 0.039
2.472AlaTyr: 2.472 ± 0.079
0.0AlaXaa: 0.0 ± 0.0
Cys
0.342CysAla: 0.342 ± 0.028
0.037CysCys: 0.037 ± 0.008
0.352CysAsp: 0.352 ± 0.028
0.249CysGlu: 0.249 ± 0.023
0.247CysPhe: 0.247 ± 0.02
0.461CysGly: 0.461 ± 0.034
0.133CysHis: 0.133 ± 0.016
0.378CysIle: 0.378 ± 0.027
0.24CysLys: 0.24 ± 0.022
0.647CysLeu: 0.647 ± 0.042
0.151CysMet: 0.151 ± 0.016
0.242CysAsn: 0.242 ± 0.02
0.228CysPro: 0.228 ± 0.021
0.208CysGln: 0.208 ± 0.02
0.162CysArg: 0.162 ± 0.017
0.374CysSer: 0.374 ± 0.027
0.269CysThr: 0.269 ± 0.023
0.313CysVal: 0.313 ± 0.024
0.08CysTrp: 0.08 ± 0.013
0.223CysTyr: 0.223 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.765AspAla: 3.765 ± 0.185
0.255AspCys: 0.255 ± 0.022
3.28AspAsp: 3.28 ± 0.101
4.216AspGlu: 4.216 ± 0.119
2.851AspPhe: 2.851 ± 0.069
3.702AspGly: 3.702 ± 0.1
1.251AspHis: 1.251 ± 0.05
4.017AspIle: 4.017 ± 0.103
4.962AspLys: 4.962 ± 0.09
5.999AspLeu: 5.999 ± 0.12
1.363AspMet: 1.363 ± 0.051
3.118AspAsn: 3.118 ± 0.109
2.55AspPro: 2.55 ± 0.084
2.644AspGln: 2.644 ± 0.09
2.146AspArg: 2.146 ± 0.066
3.204AspSer: 3.204 ± 0.088
2.769AspThr: 2.769 ± 0.077
3.617AspVal: 3.617 ± 0.085
0.713AspTrp: 0.713 ± 0.045
2.586AspTyr: 2.586 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
4.198GluAla: 4.198 ± 0.158
0.259GluCys: 0.259 ± 0.021
3.527GluAsp: 3.527 ± 0.098
4.346GluGlu: 4.346 ± 0.115
2.145GluPhe: 2.145 ± 0.068
2.892GluGly: 2.892 ± 0.068
1.152GluHis: 1.152 ± 0.048
4.739GluIle: 4.739 ± 0.111
6.017GluLys: 6.017 ± 0.113
5.74GluLeu: 5.74 ± 0.13
1.735GluMet: 1.735 ± 0.059
3.985GluAsn: 3.985 ± 0.092
1.653GluPro: 1.653 ± 0.07
2.226GluGln: 2.226 ± 0.065
2.181GluArg: 2.181 ± 0.077
3.241GluSer: 3.241 ± 0.271
2.872GluThr: 2.872 ± 0.078
4.09GluVal: 4.09 ± 0.1
0.5GluTrp: 0.5 ± 0.027
2.007GluTyr: 2.007 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
2.982PheAla: 2.982 ± 0.083
0.312PheCys: 0.312 ± 0.025
2.802PheAsp: 2.802 ± 0.076
2.31PheGlu: 2.31 ± 0.079
2.177PhePhe: 2.177 ± 0.083
3.108PheGly: 3.108 ± 0.091
0.778PheHis: 0.778 ± 0.039
3.559PheIle: 3.559 ± 0.117
3.023PheLys: 3.023 ± 0.075
4.344PheLeu: 4.344 ± 0.134
1.025PheMet: 1.025 ± 0.051
2.72PheAsn: 2.72 ± 0.074
1.357PhePro: 1.357 ± 0.049
1.157PheGln: 1.157 ± 0.05
1.222PheArg: 1.222 ± 0.048
3.222PheSer: 3.222 ± 0.1
2.628PheThr: 2.628 ± 0.067
2.86PheVal: 2.86 ± 0.083
0.604PheTrp: 0.604 ± 0.033
1.867PheTyr: 1.867 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
4.293GlyAla: 4.293 ± 0.101
0.363GlyCys: 0.363 ± 0.026
3.33GlyAsp: 3.33 ± 0.095
3.471GlyGlu: 3.471 ± 0.094
2.911GlyPhe: 2.911 ± 0.09
3.823GlyGly: 3.823 ± 0.103
1.311GlyHis: 1.311 ± 0.051
5.561GlyIle: 5.561 ± 0.125
5.336GlyLys: 5.336 ± 0.118
6.239GlyLeu: 6.239 ± 0.12
1.828GlyMet: 1.828 ± 0.06
2.998GlyAsn: 2.998 ± 0.095
1.449GlyPro: 1.449 ± 0.049
2.472GlyGln: 2.472 ± 0.082
2.305GlyArg: 2.305 ± 0.071
3.757GlySer: 3.757 ± 0.101
3.825GlyThr: 3.825 ± 0.11
4.293GlyVal: 4.293 ± 0.106
0.662GlyTrp: 0.662 ± 0.039
2.821GlyTyr: 2.821 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
1.163HisAla: 1.163 ± 0.048
0.099HisCys: 0.099 ± 0.013
1.137HisAsp: 1.137 ± 0.044
1.188HisGlu: 1.188 ± 0.054
0.963HisPhe: 0.963 ± 0.042
1.404HisGly: 1.404 ± 0.053
0.565HisHis: 0.565 ± 0.033
1.268HisIle: 1.268 ± 0.044
1.209HisLys: 1.209 ± 0.048
1.896HisLeu: 1.896 ± 0.061
0.444HisMet: 0.444 ± 0.025
0.982HisAsn: 0.982 ± 0.045
0.926HisPro: 0.926 ± 0.042
0.802HisGln: 0.802 ± 0.04
0.73HisArg: 0.73 ± 0.033
1.03HisSer: 1.03 ± 0.047
0.955HisThr: 0.955 ± 0.041
1.275HisVal: 1.275 ± 0.05
0.254HisTrp: 0.254 ± 0.021
0.919HisTyr: 0.919 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
5.857IleAla: 5.857 ± 0.115
0.606IleCys: 0.606 ± 0.037
4.696IleAsp: 4.696 ± 0.139
4.194IleGlu: 4.194 ± 0.087
3.905IlePhe: 3.905 ± 0.123
5.004IleGly: 5.004 ± 0.114
1.285IleHis: 1.285 ± 0.044
6.06IleIle: 6.06 ± 0.167
5.844IleLys: 5.844 ± 0.108
7.136IleLeu: 7.136 ± 0.173
1.718IleMet: 1.718 ± 0.074
4.61IleAsn: 4.61 ± 0.144
3.101IlePro: 3.101 ± 0.084
2.259IleGln: 2.259 ± 0.069
2.421IleArg: 2.421 ± 0.078
5.376IleSer: 5.376 ± 0.114
4.448IleThr: 4.448 ± 0.116
5.265IleVal: 5.265 ± 0.127
0.797IleTrp: 0.797 ± 0.038
2.57IleTyr: 2.57 ± 0.074
0.0IleXaa: 0.0 ± 0.0
Lys
5.153LysAla: 5.153 ± 0.176
0.242LysCys: 0.242 ± 0.021
5.333LysAsp: 5.333 ± 0.117
5.827LysGlu: 5.827 ± 0.114
2.683LysPhe: 2.683 ± 0.078
3.75LysGly: 3.75 ± 0.086
1.544LysHis: 1.544 ± 0.054
6.21LysIle: 6.21 ± 0.124
7.796LysLys: 7.796 ± 0.143
6.795LysLeu: 6.795 ± 0.122
2.468LysMet: 2.468 ± 0.071
5.294LysAsn: 5.294 ± 0.108
2.608LysPro: 2.608 ± 0.131
3.45LysGln: 3.45 ± 0.101
3.192LysArg: 3.192 ± 0.098
4.104LysSer: 4.104 ± 0.1
4.247LysThr: 4.247 ± 0.108
5.071LysVal: 5.071 ± 0.102
0.768LysTrp: 0.768 ± 0.036
3.249LysTyr: 3.249 ± 0.084
0.0LysXaa: 0.0 ± 0.0
Leu
7.423LeuAla: 7.423 ± 0.159
0.56LeuCys: 0.56 ± 0.036
5.553LeuAsp: 5.553 ± 0.119
4.987LeuGlu: 4.987 ± 0.126
4.175LeuPhe: 4.175 ± 0.124
6.293LeuGly: 6.293 ± 0.135
1.632LeuHis: 1.632 ± 0.055
7.645LeuIle: 7.645 ± 0.178
7.233LeuLys: 7.233 ± 0.134
8.787LeuLeu: 8.787 ± 0.255
2.315LeuMet: 2.315 ± 0.069
5.529LeuAsn: 5.529 ± 0.111
3.786LeuPro: 3.786 ± 0.084
3.185LeuGln: 3.185 ± 0.081
3.427LeuArg: 3.427 ± 0.088
6.661LeuSer: 6.661 ± 0.24
5.99LeuThr: 5.99 ± 0.114
6.407LeuVal: 6.407 ± 0.15
0.824LeuTrp: 0.824 ± 0.037
2.746LeuTyr: 2.746 ± 0.078
0.0LeuXaa: 0.0 ± 0.0
Met
1.971MetAla: 1.971 ± 0.062
0.146MetCys: 0.146 ± 0.015
1.352MetAsp: 1.352 ± 0.05
1.176MetGlu: 1.176 ± 0.045
0.929MetPhe: 0.929 ± 0.041
1.517MetGly: 1.517 ± 0.059
0.439MetHis: 0.439 ± 0.028
1.976MetIle: 1.976 ± 0.067
2.106MetLys: 2.106 ± 0.068
2.211MetLeu: 2.211 ± 0.071
0.803MetMet: 0.803 ± 0.036
1.476MetAsn: 1.476 ± 0.056
1.151MetPro: 1.151 ± 0.044
1.028MetGln: 1.028 ± 0.043
0.931MetArg: 0.931 ± 0.042
1.981MetSer: 1.981 ± 0.297
1.634MetThr: 1.634 ± 0.063
1.67MetVal: 1.67 ± 0.051
0.223MetTrp: 0.223 ± 0.018
0.686MetTyr: 0.686 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.626AsnAla: 3.626 ± 0.206
0.327AsnCys: 0.327 ± 0.024
3.253AsnAsp: 3.253 ± 0.095
3.333AsnGlu: 3.333 ± 0.091
2.657AsnPhe: 2.657 ± 0.074
3.995AsnGly: 3.995 ± 0.14
1.181AsnHis: 1.181 ± 0.045
3.939AsnIle: 3.939 ± 0.085
4.608AsnLys: 4.608 ± 0.084
5.403AsnLeu: 5.403 ± 0.114
1.415AsnMet: 1.415 ± 0.047
3.92AsnAsn: 3.92 ± 0.142
2.448AsnPro: 2.448 ± 0.087
2.584AsnGln: 2.584 ± 0.077
1.861AsnArg: 1.861 ± 0.063
3.987AsnSer: 3.987 ± 0.298
2.844AsnThr: 2.844 ± 0.101
3.486AsnVal: 3.486 ± 0.093
0.795AsnTrp: 0.795 ± 0.044
2.427AsnTyr: 2.427 ± 0.08
0.0AsnXaa: 0.0 ± 0.0
Pro
2.543ProAla: 2.543 ± 0.087
0.116ProCys: 0.116 ± 0.013
2.511ProAsp: 2.511 ± 0.104
2.831ProGlu: 2.831 ± 0.083
1.607ProPhe: 1.607 ± 0.061
1.956ProGly: 1.956 ± 0.087
0.662ProHis: 0.662 ± 0.032
2.674ProIle: 2.674 ± 0.069
2.701ProLys: 2.701 ± 0.074
3.008ProLeu: 3.008 ± 0.082
0.757ProMet: 0.757 ± 0.038
2.158ProAsn: 2.158 ± 0.079
0.523ProPro: 0.523 ± 0.037
1.365ProGln: 1.365 ± 0.049
1.043ProArg: 1.043 ± 0.047
1.978ProSer: 1.978 ± 0.07
2.298ProThr: 2.298 ± 0.12
2.858ProVal: 2.858 ± 0.141
0.369ProTrp: 0.369 ± 0.029
1.346ProTyr: 1.346 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
3.052GlnAla: 3.052 ± 0.116
0.092GlnCys: 0.092 ± 0.012
2.141GlnAsp: 2.141 ± 0.065
2.449GlnGlu: 2.449 ± 0.07
1.413GlnPhe: 1.413 ± 0.051
2.053GlnGly: 2.053 ± 0.061
0.609GlnHis: 0.609 ± 0.033
3.135GlnIle: 3.135 ± 0.085
3.491GlnLys: 3.491 ± 0.09
3.701GlnLeu: 3.701 ± 0.088
1.132GlnMet: 1.132 ± 0.046
2.4GlnAsn: 2.4 ± 0.081
1.203GlnPro: 1.203 ± 0.055
1.435GlnGln: 1.435 ± 0.056
1.43GlnArg: 1.43 ± 0.056
1.944GlnSer: 1.944 ± 0.065
2.327GlnThr: 2.327 ± 0.095
2.713GlnVal: 2.713 ± 0.078
0.351GlnTrp: 0.351 ± 0.024
1.421GlnTyr: 1.421 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.324ArgAla: 2.324 ± 0.061
0.175ArgCys: 0.175 ± 0.018
2.031ArgAsp: 2.031 ± 0.063
2.23ArgGlu: 2.23 ± 0.071
1.61ArgPhe: 1.61 ± 0.055
1.906ArgGly: 1.906 ± 0.062
0.809ArgHis: 0.809 ± 0.041
2.78ArgIle: 2.78 ± 0.071
3.076ArgLys: 3.076 ± 0.074
3.415ArgLeu: 3.415 ± 0.099
0.979ArgMet: 0.979 ± 0.043
1.939ArgAsn: 1.939 ± 0.056
1.297ArgPro: 1.297 ± 0.044
1.486ArgGln: 1.486 ± 0.059
1.711ArgArg: 1.711 ± 0.057
1.889ArgSer: 1.889 ± 0.068
1.661ArgThr: 1.661 ± 0.058
2.337ArgVal: 2.337 ± 0.068
0.334ArgTrp: 0.334 ± 0.022
1.425ArgTyr: 1.425 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.925SerAla: 3.925 ± 0.102
0.276SerCys: 0.276 ± 0.022
3.653SerAsp: 3.653 ± 0.093
3.871SerGlu: 3.871 ± 0.283
2.936SerPhe: 2.936 ± 0.078
4.448SerGly: 4.448 ± 0.098
1.178SerHis: 1.178 ± 0.053
4.187SerIle: 4.187 ± 0.094
4.521SerLys: 4.521 ± 0.077
6.261SerLeu: 6.261 ± 0.244
1.755SerMet: 1.755 ± 0.298
3.762SerAsn: 3.762 ± 0.309
1.818SerPro: 1.818 ± 0.055
2.376SerGln: 2.376 ± 0.076
2.141SerArg: 2.141 ± 0.074
4.325SerSer: 4.325 ± 0.135
3.529SerThr: 3.529 ± 0.101
4.092SerVal: 4.092 ± 0.278
0.814SerTrp: 0.814 ± 0.043
2.441SerTyr: 2.441 ± 0.068
0.0SerXaa: 0.0 ± 0.0
Thr
4.252ThrAla: 4.252 ± 0.159
0.293ThrCys: 0.293 ± 0.024
3.284ThrAsp: 3.284 ± 0.118
2.764ThrGlu: 2.764 ± 0.102
2.54ThrPhe: 2.54 ± 0.064
3.978ThrGly: 3.978 ± 0.109
1.013ThrHis: 1.013 ± 0.05
4.722ThrIle: 4.722 ± 0.133
4.021ThrLys: 4.021 ± 0.127
5.183ThrLeu: 5.183 ± 0.103
1.232ThrMet: 1.232 ± 0.05
3.198ThrAsn: 3.198 ± 0.146
2.642ThrPro: 2.642 ± 0.125
2.046ThrGln: 2.046 ± 0.068
1.787ThrArg: 1.787 ± 0.061
3.537ThrSer: 3.537 ± 0.087
3.641ThrThr: 3.641 ± 0.159
4.121ThrVal: 4.121 ± 0.126
0.611ThrTrp: 0.611 ± 0.041
2.181ThrTyr: 2.181 ± 0.112
0.0ThrXaa: 0.0 ± 0.0
Val
4.88ValAla: 4.88 ± 0.118
0.368ValCys: 0.368 ± 0.023
3.91ValAsp: 3.91 ± 0.088
3.827ValGlu: 3.827 ± 0.095
2.705ValPhe: 2.705 ± 0.079
4.574ValGly: 4.574 ± 0.114
1.095ValHis: 1.095 ± 0.049
5.379ValIle: 5.379 ± 0.116
5.146ValLys: 5.146 ± 0.108
6.276ValLeu: 6.276 ± 0.137
1.571ValMet: 1.571 ± 0.047
3.656ValAsn: 3.656 ± 0.108
2.741ValPro: 2.741 ± 0.117
2.3ValGln: 2.3 ± 0.07
2.177ValArg: 2.177 ± 0.069
4.642ValSer: 4.642 ± 0.295
4.22ValThr: 4.22 ± 0.179
4.829ValVal: 4.829 ± 0.132
0.642ValTrp: 0.642 ± 0.036
2.226ValTyr: 2.226 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.64TrpAla: 0.64 ± 0.039
0.082TrpCys: 0.082 ± 0.013
0.553TrpAsp: 0.553 ± 0.037
0.434TrpGlu: 0.434 ± 0.031
0.565TrpPhe: 0.565 ± 0.035
0.769TrpGly: 0.769 ± 0.042
0.277TrpHis: 0.277 ± 0.021
0.89TrpIle: 0.89 ± 0.046
0.662TrpLys: 0.662 ± 0.036
1.224TrpLeu: 1.224 ± 0.054
0.264TrpMet: 0.264 ± 0.023
0.66TrpAsn: 0.66 ± 0.043
0.31TrpPro: 0.31 ± 0.026
0.516TrpGln: 0.516 ± 0.028
0.388TrpArg: 0.388 ± 0.029
0.667TrpSer: 0.667 ± 0.035
0.528TrpThr: 0.528 ± 0.032
0.62TrpVal: 0.62 ± 0.037
0.172TrpTrp: 0.172 ± 0.019
0.434TrpTyr: 0.434 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.254TyrAla: 2.254 ± 0.06
0.264TyrCys: 0.264 ± 0.022
2.308TyrAsp: 2.308 ± 0.075
1.992TyrGlu: 1.992 ± 0.071
2.048TyrPhe: 2.048 ± 0.071
2.49TyrGly: 2.49 ± 0.059
0.974TyrHis: 0.974 ± 0.043
2.315TyrIle: 2.315 ± 0.066
2.104TyrLys: 2.104 ± 0.057
4.175TyrLeu: 4.175 ± 0.116
0.769TyrMet: 0.769 ± 0.038
1.879TyrAsn: 1.879 ± 0.06
1.479TyrPro: 1.479 ± 0.054
1.971TyrGln: 1.971 ± 0.061
1.655TyrArg: 1.655 ± 0.053
2.305TyrSer: 2.305 ± 0.06
2.135TyrThr: 2.135 ± 0.107
2.444TyrVal: 2.444 ± 0.071
0.487TyrTrp: 0.487 ± 0.029
1.568TyrTyr: 1.568 ± 0.057
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1809 proteins (587476 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski