Amino acid dipepetide frequency for Mycobacterium phage Daenerys

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.022AlaAla: 15.022 ± 1.406
0.915AlaCys: 0.915 ± 0.262
6.892AlaAsp: 6.892 ± 0.57
7.592AlaGlu: 7.592 ± 0.723
2.746AlaPhe: 2.746 ± 0.364
10.23AlaGly: 10.23 ± 1.189
2.423AlaHis: 2.423 ± 0.418
4.253AlaIle: 4.253 ± 0.442
4.577AlaLys: 4.577 ± 0.491
7.969AlaLeu: 7.969 ± 0.758
2.261AlaMet: 2.261 ± 0.352
2.8AlaAsn: 2.8 ± 0.423
4.846AlaPro: 4.846 ± 0.487
3.284AlaGln: 3.284 ± 0.404
7.538AlaArg: 7.538 ± 0.676
5.169AlaSer: 5.169 ± 0.549
5.384AlaThr: 5.384 ± 0.555
6.623AlaVal: 6.623 ± 0.623
2.154AlaTrp: 2.154 ± 0.332
2.531AlaTyr: 2.531 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
0.7CysAla: 0.7 ± 0.224
0.054CysCys: 0.054 ± 0.047
1.561CysAsp: 1.561 ± 0.372
1.131CysGlu: 1.131 ± 0.262
0.215CysPhe: 0.215 ± 0.101
1.723CysGly: 1.723 ± 0.363
0.162CysHis: 0.162 ± 0.092
0.215CysIle: 0.215 ± 0.131
0.431CysLys: 0.431 ± 0.138
0.969CysLeu: 0.969 ± 0.297
0.269CysMet: 0.269 ± 0.133
0.377CysAsn: 0.377 ± 0.139
1.077CysPro: 1.077 ± 0.264
0.538CysGln: 0.538 ± 0.193
0.861CysArg: 0.861 ± 0.219
0.7CysSer: 0.7 ± 0.19
0.754CysThr: 0.754 ± 0.225
0.7CysVal: 0.7 ± 0.18
0.377CysTrp: 0.377 ± 0.149
0.162CysTyr: 0.162 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
6.784AspAla: 6.784 ± 0.678
1.185AspCys: 1.185 ± 0.261
4.523AspAsp: 4.523 ± 0.592
3.284AspGlu: 3.284 ± 0.428
1.938AspPhe: 1.938 ± 0.255
6.299AspGly: 6.299 ± 0.609
1.508AspHis: 1.508 ± 0.343
2.584AspIle: 2.584 ± 0.313
1.723AspLys: 1.723 ± 0.281
6.084AspLeu: 6.084 ± 0.476
1.238AspMet: 1.238 ± 0.281
1.669AspAsn: 1.669 ± 0.356
4.684AspPro: 4.684 ± 0.433
2.746AspGln: 2.746 ± 0.363
5.007AspArg: 5.007 ± 0.55
3.392AspSer: 3.392 ± 0.542
3.715AspThr: 3.715 ± 0.516
4.738AspVal: 4.738 ± 0.582
1.508AspTrp: 1.508 ± 0.302
2.046AspTyr: 2.046 ± 0.3
0.0AspXaa: 0.0 ± 0.0
Glu
6.407GluAla: 6.407 ± 0.625
0.915GluCys: 0.915 ± 0.229
2.907GluAsp: 2.907 ± 0.405
2.746GluGlu: 2.746 ± 0.481
2.423GluPhe: 2.423 ± 0.344
3.715GluGly: 3.715 ± 0.377
1.777GluHis: 1.777 ± 0.349
2.584GluIle: 2.584 ± 0.414
1.938GluLys: 1.938 ± 0.353
5.761GluLeu: 5.761 ± 0.7
1.454GluMet: 1.454 ± 0.28
1.831GluAsn: 1.831 ± 0.315
3.069GluPro: 3.069 ± 0.431
2.8GluGln: 2.8 ± 0.395
5.169GluArg: 5.169 ± 0.592
3.284GluSer: 3.284 ± 0.448
4.684GluThr: 4.684 ± 0.609
3.661GluVal: 3.661 ± 0.448
1.615GluTrp: 1.615 ± 0.319
1.669GluTyr: 1.669 ± 0.289
0.0GluXaa: 0.0 ± 0.0
Phe
2.854PheAla: 2.854 ± 0.393
0.377PheCys: 0.377 ± 0.136
2.315PheAsp: 2.315 ± 0.434
2.046PheGlu: 2.046 ± 0.319
1.023PhePhe: 1.023 ± 0.234
3.177PheGly: 3.177 ± 0.74
0.431PheHis: 0.431 ± 0.149
1.292PheIle: 1.292 ± 0.327
1.185PheLys: 1.185 ± 0.291
1.615PheLeu: 1.615 ± 0.258
0.592PheMet: 0.592 ± 0.154
1.131PheAsn: 1.131 ± 0.306
1.615PhePro: 1.615 ± 0.315
1.292PheGln: 1.292 ± 0.274
1.615PheArg: 1.615 ± 0.295
1.4PheSer: 1.4 ± 0.299
2.046PheThr: 2.046 ± 0.368
1.938PheVal: 1.938 ± 0.306
0.431PheTrp: 0.431 ± 0.134
0.754PheTyr: 0.754 ± 0.254
0.0PheXaa: 0.0 ± 0.0
Gly
9.799GlyAla: 9.799 ± 1.063
0.969GlyCys: 0.969 ± 0.264
6.246GlyAsp: 6.246 ± 0.493
4.415GlyGlu: 4.415 ± 0.589
2.8GlyPhe: 2.8 ± 0.514
11.576GlyGly: 11.576 ± 2.076
2.154GlyHis: 2.154 ± 0.297
4.038GlyIle: 4.038 ± 0.574
2.477GlyLys: 2.477 ± 0.307
6.192GlyLeu: 6.192 ± 0.62
2.261GlyMet: 2.261 ± 0.467
3.177GlyAsn: 3.177 ± 0.433
4.307GlyPro: 4.307 ± 0.642
2.423GlyGln: 2.423 ± 0.531
5.384GlyArg: 5.384 ± 0.587
6.03GlySer: 6.03 ± 0.992
6.892GlyThr: 6.892 ± 0.772
5.869GlyVal: 5.869 ± 0.649
2.369GlyTrp: 2.369 ± 0.353
1.938GlyTyr: 1.938 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
1.669HisAla: 1.669 ± 0.335
0.431HisCys: 0.431 ± 0.183
0.969HisAsp: 0.969 ± 0.233
1.185HisGlu: 1.185 ± 0.255
0.377HisPhe: 0.377 ± 0.115
1.454HisGly: 1.454 ± 0.28
0.861HisHis: 0.861 ± 0.217
1.615HisIle: 1.615 ± 0.32
0.754HisLys: 0.754 ± 0.213
1.723HisLeu: 1.723 ± 0.285
0.485HisMet: 0.485 ± 0.135
0.915HisAsn: 0.915 ± 0.214
1.615HisPro: 1.615 ± 0.302
0.808HisGln: 0.808 ± 0.264
2.208HisArg: 2.208 ± 0.398
0.969HisSer: 0.969 ± 0.228
1.4HisThr: 1.4 ± 0.365
1.454HisVal: 1.454 ± 0.3
0.538HisTrp: 0.538 ± 0.163
1.023HisTyr: 1.023 ± 0.223
0.0HisXaa: 0.0 ± 0.0
Ile
5.061IleAla: 5.061 ± 0.497
0.7IleCys: 0.7 ± 0.224
3.984IleAsp: 3.984 ± 0.505
3.554IleGlu: 3.554 ± 0.363
0.7IlePhe: 0.7 ± 0.209
4.038IleGly: 4.038 ± 0.42
1.238IleHis: 1.238 ± 0.268
1.615IleIle: 1.615 ± 0.267
0.969IleLys: 0.969 ± 0.206
2.423IleLeu: 2.423 ± 0.455
0.377IleMet: 0.377 ± 0.118
2.046IleAsn: 2.046 ± 0.312
2.854IlePro: 2.854 ± 0.363
1.4IleGln: 1.4 ± 0.264
2.423IleArg: 2.423 ± 0.388
2.1IleSer: 2.1 ± 0.409
3.392IleThr: 3.392 ± 0.417
3.23IleVal: 3.23 ± 0.384
1.023IleTrp: 1.023 ± 0.244
0.808IleTyr: 0.808 ± 0.18
0.0IleXaa: 0.0 ± 0.0
Lys
3.769LysAla: 3.769 ± 0.494
0.646LysCys: 0.646 ± 0.189
1.669LysAsp: 1.669 ± 0.291
1.4LysGlu: 1.4 ± 0.241
1.185LysPhe: 1.185 ± 0.198
2.477LysGly: 2.477 ± 0.383
0.861LysHis: 0.861 ± 0.255
0.861LysIle: 0.861 ± 0.238
1.508LysLys: 1.508 ± 0.377
2.531LysLeu: 2.531 ± 0.383
0.538LysMet: 0.538 ± 0.133
0.861LysAsn: 0.861 ± 0.198
2.584LysPro: 2.584 ± 0.395
1.777LysGln: 1.777 ± 0.313
2.692LysArg: 2.692 ± 0.455
2.046LysSer: 2.046 ± 0.298
1.992LysThr: 1.992 ± 0.257
2.369LysVal: 2.369 ± 0.336
1.023LysTrp: 1.023 ± 0.223
0.754LysTyr: 0.754 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
7.807LeuAla: 7.807 ± 0.665
0.969LeuCys: 0.969 ± 0.252
5.061LeuAsp: 5.061 ± 0.505
4.577LeuGlu: 4.577 ± 0.53
2.315LeuPhe: 2.315 ± 0.287
4.523LeuGly: 4.523 ± 0.495
0.969LeuHis: 0.969 ± 0.26
3.123LeuIle: 3.123 ± 0.412
2.477LeuLys: 2.477 ± 0.356
5.384LeuLeu: 5.384 ± 0.602
1.831LeuMet: 1.831 ± 0.302
2.531LeuAsn: 2.531 ± 0.327
5.007LeuPro: 5.007 ± 0.643
2.8LeuGln: 2.8 ± 0.471
5.33LeuArg: 5.33 ± 0.767
4.846LeuSer: 4.846 ± 0.5
5.061LeuThr: 5.061 ± 0.445
5.546LeuVal: 5.546 ± 0.582
1.238LeuTrp: 1.238 ± 0.283
2.154LeuTyr: 2.154 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.046MetAla: 2.046 ± 0.338
0.215MetCys: 0.215 ± 0.125
1.346MetAsp: 1.346 ± 0.296
1.023MetGlu: 1.023 ± 0.19
0.646MetPhe: 0.646 ± 0.174
1.992MetGly: 1.992 ± 0.257
0.108MetHis: 0.108 ± 0.08
0.861MetIle: 0.861 ± 0.24
0.646MetLys: 0.646 ± 0.184
1.561MetLeu: 1.561 ± 0.292
0.485MetMet: 0.485 ± 0.211
1.077MetAsn: 1.077 ± 0.252
1.185MetPro: 1.185 ± 0.243
0.592MetGln: 0.592 ± 0.149
1.454MetArg: 1.454 ± 0.299
2.854MetSer: 2.854 ± 0.362
1.723MetThr: 1.723 ± 0.303
1.185MetVal: 1.185 ± 0.306
0.162MetTrp: 0.162 ± 0.087
0.269MetTyr: 0.269 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
3.284AsnAla: 3.284 ± 0.37
0.215AsnCys: 0.215 ± 0.111
1.831AsnAsp: 1.831 ± 0.333
1.992AsnGlu: 1.992 ± 0.385
0.808AsnPhe: 0.808 ± 0.255
4.092AsnGly: 4.092 ± 0.508
0.969AsnHis: 0.969 ± 0.212
1.615AsnIle: 1.615 ± 0.418
0.969AsnLys: 0.969 ± 0.246
2.477AsnLeu: 2.477 ± 0.384
0.538AsnMet: 0.538 ± 0.151
1.723AsnAsn: 1.723 ± 0.339
2.584AsnPro: 2.584 ± 0.389
1.077AsnGln: 1.077 ± 0.315
1.884AsnArg: 1.884 ± 0.36
1.615AsnSer: 1.615 ± 0.334
1.992AsnThr: 1.992 ± 0.294
2.154AsnVal: 2.154 ± 0.339
0.969AsnTrp: 0.969 ± 0.204
0.592AsnTyr: 0.592 ± 0.147
0.0AsnXaa: 0.0 ± 0.0
Pro
5.276ProAla: 5.276 ± 0.566
0.7ProCys: 0.7 ± 0.196
4.684ProAsp: 4.684 ± 0.512
4.253ProGlu: 4.253 ± 0.417
1.615ProPhe: 1.615 ± 0.36
6.515ProGly: 6.515 ± 0.778
1.454ProHis: 1.454 ± 0.266
2.154ProIle: 2.154 ± 0.333
2.261ProLys: 2.261 ± 0.346
3.877ProLeu: 3.877 ± 0.619
1.346ProMet: 1.346 ± 0.327
2.1ProAsn: 2.1 ± 0.321
4.2ProPro: 4.2 ± 0.612
2.208ProGln: 2.208 ± 0.365
3.338ProArg: 3.338 ± 0.52
3.177ProSer: 3.177 ± 0.477
3.554ProThr: 3.554 ± 0.465
4.738ProVal: 4.738 ± 0.525
1.292ProTrp: 1.292 ± 0.234
1.669ProTyr: 1.669 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
4.146GlnAla: 4.146 ± 0.631
0.538GlnCys: 0.538 ± 0.25
1.831GlnAsp: 1.831 ± 0.283
1.938GlnGlu: 1.938 ± 0.331
1.185GlnPhe: 1.185 ± 0.213
2.477GlnGly: 2.477 ± 0.469
0.861GlnHis: 0.861 ± 0.217
1.615GlnIle: 1.615 ± 0.288
1.346GlnLys: 1.346 ± 0.263
2.961GlnLeu: 2.961 ± 0.392
0.7GlnMet: 0.7 ± 0.21
0.861GlnAsn: 0.861 ± 0.238
2.692GlnPro: 2.692 ± 0.404
1.508GlnGln: 1.508 ± 0.358
2.638GlnArg: 2.638 ± 0.386
2.154GlnSer: 2.154 ± 0.351
1.615GlnThr: 1.615 ± 0.33
2.477GlnVal: 2.477 ± 0.359
0.7GlnTrp: 0.7 ± 0.195
1.023GlnTyr: 1.023 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
6.946ArgAla: 6.946 ± 0.649
1.131ArgCys: 1.131 ± 0.262
4.738ArgAsp: 4.738 ± 0.621
5.007ArgGlu: 5.007 ± 0.566
1.938ArgPhe: 1.938 ± 0.356
4.307ArgGly: 4.307 ± 0.501
1.454ArgHis: 1.454 ± 0.297
4.092ArgIle: 4.092 ± 0.511
2.154ArgLys: 2.154 ± 0.345
4.738ArgLeu: 4.738 ± 0.539
2.208ArgMet: 2.208 ± 0.39
2.531ArgAsn: 2.531 ± 0.33
3.823ArgPro: 3.823 ± 0.439
1.938ArgGln: 1.938 ± 0.378
5.815ArgArg: 5.815 ± 0.787
3.5ArgSer: 3.5 ± 0.37
3.392ArgThr: 3.392 ± 0.549
5.223ArgVal: 5.223 ± 0.659
2.046ArgTrp: 2.046 ± 0.337
1.938ArgTyr: 1.938 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
5.707SerAla: 5.707 ± 0.784
0.538SerCys: 0.538 ± 0.215
3.984SerAsp: 3.984 ± 0.467
2.907SerGlu: 2.907 ± 0.329
1.777SerPhe: 1.777 ± 0.395
6.353SerGly: 6.353 ± 1.011
1.4SerHis: 1.4 ± 0.285
2.692SerIle: 2.692 ± 0.417
1.992SerLys: 1.992 ± 0.328
3.554SerLeu: 3.554 ± 0.372
1.292SerMet: 1.292 ± 0.226
2.369SerAsn: 2.369 ± 0.451
3.123SerPro: 3.123 ± 0.373
1.508SerGln: 1.508 ± 0.218
3.338SerArg: 3.338 ± 0.351
3.661SerSer: 3.661 ± 0.71
3.715SerThr: 3.715 ± 0.464
4.738SerVal: 4.738 ± 0.639
1.238SerTrp: 1.238 ± 0.216
1.346SerTyr: 1.346 ± 0.272
0.0SerXaa: 0.0 ± 0.0
Thr
6.084ThrAla: 6.084 ± 0.58
0.538ThrCys: 0.538 ± 0.2
3.715ThrAsp: 3.715 ± 0.612
3.769ThrGlu: 3.769 ± 0.446
1.777ThrPhe: 1.777 ± 0.289
7.107ThrGly: 7.107 ± 0.711
1.561ThrHis: 1.561 ± 0.343
3.715ThrIle: 3.715 ± 0.442
2.1ThrLys: 2.1 ± 0.339
4.415ThrLeu: 4.415 ± 0.558
1.131ThrMet: 1.131 ± 0.209
1.992ThrAsn: 1.992 ± 0.442
4.146ThrPro: 4.146 ± 0.516
2.154ThrGln: 2.154 ± 0.381
3.5ThrArg: 3.5 ± 0.482
3.93ThrSer: 3.93 ± 0.429
4.738ThrThr: 4.738 ± 0.648
5.33ThrVal: 5.33 ± 0.553
1.185ThrTrp: 1.185 ± 0.288
1.508ThrTyr: 1.508 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
7.43ValAla: 7.43 ± 0.599
1.454ValCys: 1.454 ± 0.264
5.384ValAsp: 5.384 ± 0.588
4.469ValGlu: 4.469 ± 0.613
2.261ValPhe: 2.261 ± 0.368
5.869ValGly: 5.869 ± 0.683
1.077ValHis: 1.077 ± 0.204
2.746ValIle: 2.746 ± 0.396
2.8ValLys: 2.8 ± 0.423
5.223ValLeu: 5.223 ± 0.606
1.346ValMet: 1.346 ± 0.237
2.046ValAsn: 2.046 ± 0.305
3.984ValPro: 3.984 ± 0.389
2.638ValGln: 2.638 ± 0.359
4.523ValArg: 4.523 ± 0.516
4.469ValSer: 4.469 ± 0.637
5.169ValThr: 5.169 ± 0.535
6.299ValVal: 6.299 ± 0.714
1.831ValTrp: 1.831 ± 0.334
1.185ValTyr: 1.185 ± 0.25
0.0ValXaa: 0.0 ± 0.0
Trp
1.992TrpAla: 1.992 ± 0.314
0.162TrpCys: 0.162 ± 0.087
1.508TrpAsp: 1.508 ± 0.263
1.185TrpGlu: 1.185 ± 0.294
0.538TrpPhe: 0.538 ± 0.155
1.238TrpGly: 1.238 ± 0.28
0.7TrpHis: 0.7 ± 0.195
1.023TrpIle: 1.023 ± 0.219
0.7TrpLys: 0.7 ± 0.187
2.154TrpLeu: 2.154 ± 0.38
0.915TrpMet: 0.915 ± 0.245
0.754TrpAsn: 0.754 ± 0.224
1.561TrpPro: 1.561 ± 0.367
0.969TrpGln: 0.969 ± 0.262
2.261TrpArg: 2.261 ± 0.442
1.131TrpSer: 1.131 ± 0.261
1.4TrpThr: 1.4 ± 0.251
1.777TrpVal: 1.777 ± 0.357
0.969TrpTrp: 0.969 ± 0.189
0.323TrpTyr: 0.323 ± 0.113
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.423TyrAla: 2.423 ± 0.426
0.323TyrCys: 0.323 ± 0.148
1.508TyrAsp: 1.508 ± 0.287
1.938TyrGlu: 1.938 ± 0.305
0.861TyrPhe: 0.861 ± 0.256
2.154TyrGly: 2.154 ± 0.433
0.485TyrHis: 0.485 ± 0.144
1.238TyrIle: 1.238 ± 0.267
0.538TyrLys: 0.538 ± 0.175
1.938TyrLeu: 1.938 ± 0.327
0.108TyrMet: 0.108 ± 0.077
0.592TyrAsn: 0.592 ± 0.146
1.454TyrPro: 1.454 ± 0.3
0.808TyrGln: 0.808 ± 0.213
1.992TyrArg: 1.992 ± 0.342
0.808TyrSer: 0.808 ± 0.236
1.777TyrThr: 1.777 ± 0.316
2.154TyrVal: 2.154 ± 0.338
0.592TyrTrp: 0.592 ± 0.157
0.646TyrTyr: 0.646 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (18574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski