Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium glutamicum

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3094 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8NQT0|Q8NQT0_CORGL Uncharacterized protein OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=Cgl1344 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 9.92DD3 pKa = 3.48YY4 pKa = 11.05AVHH7 pKa = 5.54TRR9 pKa = 11.84GLVSLLSLIFLFVLSGCGGNATTADD34 pKa = 3.69EE35 pKa = 4.37AAEE38 pKa = 4.11SDD40 pKa = 4.13VVTSSSAPPSKK51 pKa = 10.21RR52 pKa = 11.84ALDD55 pKa = 3.35VGEE58 pKa = 4.18AVEE61 pKa = 4.26IPGVVLTVNSVTQSDD76 pKa = 3.68QLMLYY81 pKa = 10.17SEE83 pKa = 5.07GSARR87 pKa = 11.84GSEE90 pKa = 3.87PRR92 pKa = 11.84EE93 pKa = 3.79QRR95 pKa = 11.84NAASGEE101 pKa = 4.14KK102 pKa = 9.73FVSVDD107 pKa = 3.38TTVKK111 pKa = 10.65NSSSDD116 pKa = 3.36PWDD119 pKa = 3.78LSCGHH124 pKa = 6.6VLQTWLLEE132 pKa = 4.58DD133 pKa = 4.73EE134 pKa = 5.95LDD136 pKa = 4.32DD137 pKa = 4.66QQGDD141 pKa = 3.77QEE143 pKa = 5.03KK144 pKa = 10.48KK145 pKa = 10.49FSPIDD150 pKa = 3.5NLDD153 pKa = 3.78QISGNPEE160 pKa = 3.79CGVLLEE166 pKa = 4.38VGTEE170 pKa = 4.48IEE172 pKa = 4.47MTWSFTIPDD181 pKa = 5.04DD182 pKa = 4.59IEE184 pKa = 3.86ITHH187 pKa = 6.81FGFSLSDD194 pKa = 3.36STSNDD199 pKa = 2.94LAIISLGGAIEE210 pKa = 4.17TSSAITTTEE219 pKa = 3.87VIAPEE224 pKa = 4.16NDD226 pKa = 3.17TEE228 pKa = 4.35TLLEE232 pKa = 4.17ITPVDD237 pKa = 3.99CQVGLGPIVTSWSDD251 pKa = 3.27GTVGGWSQHH260 pKa = 5.58CQDD263 pKa = 3.44VHH265 pKa = 8.84DD266 pKa = 4.55EE267 pKa = 4.19VLAGEE272 pKa = 4.57VAANTPVCDD281 pKa = 4.13GVVCTYY287 pKa = 10.74PSGATMPDD295 pKa = 3.33PNAPQIPSDD304 pKa = 3.56TSGAVCDD311 pKa = 4.57EE312 pKa = 4.33NQCVYY317 pKa = 10.6PNGYY321 pKa = 8.41IARR324 pKa = 11.84IGDD327 pKa = 3.94PNVPNYY333 pKa = 9.87LKK335 pKa = 10.35PGNSPWVQGQIDD347 pKa = 4.06FQNCLDD353 pKa = 3.69SGKK356 pKa = 9.11TIEE359 pKa = 4.16QCRR362 pKa = 11.84EE363 pKa = 3.7EE364 pKa = 4.47LNN366 pKa = 3.6

Molecular weight: 38.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q79VC7|Q79VC7_CORGL Permeases of the major facilitator superfamily OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=Cgl2777 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.72GRR42 pKa = 11.84AKK44 pKa = 9.63LTAA47 pKa = 4.21

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3093

1

3094

958486

17

2993

309.8

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.723 ± 0.049

0.669 ± 0.013

5.945 ± 0.04

6.354 ± 0.041

3.635 ± 0.029

8.034 ± 0.04

2.12 ± 0.021

5.775 ± 0.036

3.487 ± 0.032

9.673 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.018

3.295 ± 0.025

4.842 ± 0.032

3.383 ± 0.029

5.596 ± 0.039

6.393 ± 0.032

6.172 ± 0.036

7.951 ± 0.039

1.411 ± 0.018

2.22 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski