Alphaproteobacteria bacterium PA3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4749 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257JLY2|A0A257JLY2_9PROT Uncharacterized protein OS=Alphaproteobacteria bacterium PA3 OX=2015571 GN=CFE32_14480 PE=3 SV=1
MM1 pKa = 7.76CIVCQAKK8 pKa = 10.47SILKK12 pKa = 9.6PDD14 pKa = 3.42YY15 pKa = 10.56EE16 pKa = 5.12LIFGSGMNDD25 pKa = 2.95VPAAAALASKK35 pKa = 10.47EE36 pKa = 3.9PDD38 pKa = 3.46YY39 pKa = 11.71LEE41 pKa = 4.29TANATTSNTATSDD54 pKa = 3.44QNINGVLANTRR65 pKa = 11.84FSSLTLTFAFPQLATDD81 pKa = 3.74YY82 pKa = 8.49EE83 pKa = 4.35ANYY86 pKa = 10.5ASVSATTSQVIGSFSPITAAMQTAARR112 pKa = 11.84FTFEE116 pKa = 5.18LASQLTGLTITEE128 pKa = 4.46GPAATANFRR137 pKa = 11.84LGLFEE142 pKa = 4.56QNADD146 pKa = 3.19GTGPRR151 pKa = 11.84TAFAYY156 pKa = 10.48LPTSSIVGGDD166 pKa = 2.87SWYY169 pKa = 11.16SNTSSFTSPTRR180 pKa = 11.84GNYY183 pKa = 8.41AWATILHH190 pKa = 6.6EE191 pKa = 4.78IGHH194 pKa = 6.09NLGLKK199 pKa = 9.7HH200 pKa = 5.41GHH202 pKa = 5.29EE203 pKa = 4.68TGGPAGVAMAANRR216 pKa = 11.84NSMEE220 pKa = 4.24FSLMTYY226 pKa = 10.71NSFIGDD232 pKa = 3.49DD233 pKa = 3.44TAGYY237 pKa = 8.49TNEE240 pKa = 3.78RR241 pKa = 11.84WGFAQTFMMYY251 pKa = 10.36DD252 pKa = 3.5IAALQHH258 pKa = 6.13MYY260 pKa = 10.81GADD263 pKa = 3.03FTMNNTNSIYY273 pKa = 10.37TFSEE277 pKa = 4.15TTGEE281 pKa = 3.98MFINGVGQGAPGGNRR296 pKa = 11.84VFLTIWDD303 pKa = 3.91GGGTDD308 pKa = 3.99TYY310 pKa = 11.6DD311 pKa = 3.3FSNYY315 pKa = 7.17TTTQLIDD322 pKa = 3.69LRR324 pKa = 11.84PSFWSKK330 pKa = 10.65FSDD333 pKa = 3.55VQTANLGSGNFSRR346 pKa = 11.84GNVFNALQYY355 pKa = 10.89QNDD358 pKa = 3.54GRR360 pKa = 11.84SLIEE364 pKa = 3.76NAFGGSGNDD373 pKa = 4.51DD374 pKa = 2.84IRR376 pKa = 11.84GNQAANTLEE385 pKa = 4.13GRR387 pKa = 11.84DD388 pKa = 3.99GNDD391 pKa = 4.14LINGGSGADD400 pKa = 3.48FLYY403 pKa = 10.71GQVGNDD409 pKa = 3.42TLIGDD414 pKa = 4.25FVVTNSVAANPGVGFTGLAIQTLAQGSAPTSQSTALNLTQNFGLFSNSDD463 pKa = 3.19IGDD466 pKa = 3.93STTSPHH472 pKa = 4.75TTISASGNGAAHH484 pKa = 7.79WFRR487 pKa = 11.84VDD489 pKa = 2.89IASADD494 pKa = 3.53VTITLDD500 pKa = 3.15IDD502 pKa = 3.59ATTGGLDD509 pKa = 3.18SFVRR513 pKa = 11.84LFFLAPGGTIATKK526 pKa = 10.47VAEE529 pKa = 4.46NDD531 pKa = 4.02DD532 pKa = 4.2GSTSAGGGGSATNTDD547 pKa = 3.12SYY549 pKa = 11.07LTYY552 pKa = 10.04LTLQPGTYY560 pKa = 8.78FAVVGTYY567 pKa = 9.78QDD569 pKa = 2.94IDD571 pKa = 3.59ALASGTGYY579 pKa = 8.47TLHH582 pKa = 7.04ISVRR586 pKa = 11.84GEE588 pKa = 3.78VSTTGLGDD596 pKa = 3.52GGTAGDD602 pKa = 3.7DD603 pKa = 3.29QLYY606 pKa = 10.7GGEE609 pKa = 4.62GNDD612 pKa = 3.73TLIGGLGADD621 pKa = 3.74RR622 pKa = 11.84LEE624 pKa = 4.56GQNGVDD630 pKa = 3.18TASYY634 pKa = 10.74ANAATGVYY642 pKa = 10.22VGFGDD647 pKa = 3.64SGNATGEE654 pKa = 4.12AFGDD658 pKa = 3.64TFFSIEE664 pKa = 4.28NLTGSAFSDD673 pKa = 3.06ILSWDD678 pKa = 3.19NNANVISGLAGQDD691 pKa = 3.22QLFGNGGNDD700 pKa = 3.23TLLGGTEE707 pKa = 4.54DD708 pKa = 4.42DD709 pKa = 4.42VLTGGQGGDD718 pKa = 3.62VLNGEE723 pKa = 4.75AGFDD727 pKa = 3.62TASYY731 pKa = 9.54AASAAAIYY739 pKa = 10.38LFAGDD744 pKa = 3.73NGNWTGDD751 pKa = 3.32AFGDD755 pKa = 3.64TFISIEE761 pKa = 4.2RR762 pKa = 11.84VDD764 pKa = 3.64GSNFHH769 pKa = 7.04DD770 pKa = 4.94LIGFSEE776 pKa = 4.63FNDD779 pKa = 3.62EE780 pKa = 4.37VRR782 pKa = 11.84GLAGNDD788 pKa = 3.16QLYY791 pKa = 11.02GLGGDD796 pKa = 4.02DD797 pKa = 3.79VLLGGEE803 pKa = 4.91GRR805 pKa = 11.84DD806 pKa = 3.8LLNGGSGTDD815 pKa = 3.26TASYY819 pKa = 8.22ATSAAAIYY827 pKa = 10.07LVAGDD832 pKa = 3.93EE833 pKa = 4.61GNWTGDD839 pKa = 3.16AFGDD843 pKa = 3.67TLISIEE849 pKa = 4.58RR850 pKa = 11.84VDD852 pKa = 3.7GSNFNDD858 pKa = 3.96IIGLSDD864 pKa = 3.52LADD867 pKa = 3.66EE868 pKa = 4.69VRR870 pKa = 11.84GLSGNDD876 pKa = 3.07QLFGLGGNDD885 pKa = 3.15ILIGGIGRR893 pKa = 11.84DD894 pKa = 3.64VLNGGTGTDD903 pKa = 3.21TASYY907 pKa = 8.21STAAAGVYY915 pKa = 10.22AFAGDD920 pKa = 3.39WGNFTGDD927 pKa = 3.26AAGDD931 pKa = 3.7TFTSIEE937 pKa = 4.33NLTGSAFNDD946 pKa = 3.25ILGMDD951 pKa = 4.56GGNNVIDD958 pKa = 4.47GGAGDD963 pKa = 5.38DD964 pKa = 3.69VLFGQGGGDD973 pKa = 3.68TLIGGAGFDD982 pKa = 3.55TASYY986 pKa = 8.61ATATSGVYY994 pKa = 9.67VFYY997 pKa = 10.98GDD999 pKa = 3.18WGSFTGDD1006 pKa = 2.99ARR1008 pKa = 11.84GDD1010 pKa = 3.48TMFSVEE1016 pKa = 5.27SILGSNFNDD1025 pKa = 4.0IIGMDD1030 pKa = 3.91DD1031 pKa = 4.03FANTLAGGGGDD1042 pKa = 4.25DD1043 pKa = 3.37ILYY1046 pKa = 10.95GEE1048 pKa = 4.87GGGDD1052 pKa = 3.15RR1053 pKa = 11.84LFGNAGFDD1061 pKa = 3.53TVSYY1065 pKa = 10.79AFAPSGVYY1073 pKa = 10.03ILFSDD1078 pKa = 4.7LGNATGDD1085 pKa = 3.41AAGDD1089 pKa = 4.04SEE1091 pKa = 4.8FSSIEE1096 pKa = 4.29CIVGSAFDD1104 pKa = 5.5DD1105 pKa = 4.35IIGMGDD1111 pKa = 3.37FNDD1114 pKa = 3.97TLGGGGGNDD1123 pKa = 2.82VLMGNGGGDD1132 pKa = 2.78RR1133 pKa = 11.84FFGNEE1138 pKa = 3.49GFDD1141 pKa = 3.52TVSYY1145 pKa = 10.3GLAGSGVYY1153 pKa = 10.06IFFADD1158 pKa = 4.31LGNATGDD1165 pKa = 3.35ARR1167 pKa = 11.84GDD1169 pKa = 3.84SEE1171 pKa = 4.25FSSIEE1176 pKa = 4.24SIVGSNFNDD1185 pKa = 3.81VIGMGDD1191 pKa = 3.45FADD1194 pKa = 4.55TIIGGGGNDD1203 pKa = 3.4ILLGMGGNDD1212 pKa = 4.06TLWGGAGNDD1221 pKa = 3.34LFAYY1225 pKa = 9.43TSFFFGTDD1233 pKa = 3.7TIRR1236 pKa = 11.84DD1237 pKa = 3.89FQDD1240 pKa = 3.32GLDD1243 pKa = 4.16RR1244 pKa = 11.84IDD1246 pKa = 4.07FSRR1249 pKa = 11.84LAGATYY1255 pKa = 10.49SGLAITSVAGGTQVALGTSTILLSGINTSQITAADD1290 pKa = 3.71FLFAA1294 pKa = 5.89

Molecular weight: 132.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257JVV1|A0A257JVV1_9PROT Heat-inducible transcription repressor HrcA OS=Alphaproteobacteria bacterium PA3 OX=2015571 GN=hrcA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84ARR12 pKa = 11.84MATKK16 pKa = 10.32GGRR19 pKa = 11.84LVLARR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AKK28 pKa = 9.93GRR30 pKa = 11.84KK31 pKa = 8.85RR32 pKa = 11.84LSAA35 pKa = 3.96

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4749

0

4749

1247484

16

2327

262.7

28.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.68 ± 0.054

0.853 ± 0.013

5.637 ± 0.032

5.318 ± 0.038

3.789 ± 0.023

8.573 ± 0.046

1.879 ± 0.017

5.069 ± 0.026

3.556 ± 0.027

10.071 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.021

2.851 ± 0.026

5.246 ± 0.026

3.568 ± 0.025

6.524 ± 0.033

5.805 ± 0.027

5.536 ± 0.033

7.079 ± 0.029

1.445 ± 0.017

2.146 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski