Amino acid dipepetide frequency for Serratia phage Scapp

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.78AlaAla: 11.78 ± 1.548
1.185AlaCys: 1.185 ± 0.246
7.039AlaAsp: 7.039 ± 0.735
6.594AlaGlu: 6.594 ± 0.829
4.149AlaPhe: 4.149 ± 0.665
7.187AlaGly: 7.187 ± 0.767
1.778AlaHis: 1.778 ± 0.359
5.483AlaIle: 5.483 ± 0.635
6.298AlaLys: 6.298 ± 0.927
7.557AlaLeu: 7.557 ± 0.929
2.297AlaMet: 2.297 ± 0.407
3.779AlaAsn: 3.779 ± 0.555
3.63AlaPro: 3.63 ± 0.515
5.038AlaGln: 5.038 ± 0.605
6.372AlaArg: 6.372 ± 0.899
5.186AlaSer: 5.186 ± 0.509
6.298AlaThr: 6.298 ± 1.067
6.965AlaVal: 6.965 ± 0.745
1.482AlaTrp: 1.482 ± 0.368
3.408AlaTyr: 3.408 ± 0.429
0.0AlaXaa: 0.0 ± 0.0
Cys
0.963CysAla: 0.963 ± 0.274
0.296CysCys: 0.296 ± 0.156
0.667CysAsp: 0.667 ± 0.21
0.963CysGlu: 0.963 ± 0.303
0.296CysPhe: 0.296 ± 0.135
1.111CysGly: 1.111 ± 0.349
0.222CysHis: 0.222 ± 0.137
0.667CysIle: 0.667 ± 0.23
0.815CysLys: 0.815 ± 0.291
0.741CysLeu: 0.741 ± 0.279
0.37CysMet: 0.37 ± 0.134
0.519CysAsn: 0.519 ± 0.194
0.296CysPro: 0.296 ± 0.183
0.148CysGln: 0.148 ± 0.102
1.111CysArg: 1.111 ± 0.329
0.815CysSer: 0.815 ± 0.237
0.593CysThr: 0.593 ± 0.188
0.667CysVal: 0.667 ± 0.187
0.296CysTrp: 0.296 ± 0.151
0.445CysTyr: 0.445 ± 0.205
0.0CysXaa: 0.0 ± 0.0
Asp
6.816AspAla: 6.816 ± 0.687
0.593AspCys: 0.593 ± 0.258
5.112AspAsp: 5.112 ± 1.72
5.631AspGlu: 5.631 ± 1.008
2.667AspPhe: 2.667 ± 0.407
5.186AspGly: 5.186 ± 0.65
0.741AspHis: 0.741 ± 0.222
2.89AspIle: 2.89 ± 0.382
3.408AspLys: 3.408 ± 0.563
3.853AspLeu: 3.853 ± 0.635
1.926AspMet: 1.926 ± 0.445
2.815AspAsn: 2.815 ± 0.386
2.667AspPro: 2.667 ± 0.407
1.185AspGln: 1.185 ± 0.295
2.593AspArg: 2.593 ± 0.398
2.741AspSer: 2.741 ± 0.466
3.038AspThr: 3.038 ± 0.413
5.631AspVal: 5.631 ± 0.596
1.111AspTrp: 1.111 ± 0.279
2.075AspTyr: 2.075 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
5.705GluAla: 5.705 ± 0.623
0.741GluCys: 0.741 ± 0.266
4.075GluAsp: 4.075 ± 0.681
3.705GluGlu: 3.705 ± 1.37
3.186GluPhe: 3.186 ± 0.526
2.519GluGly: 2.519 ± 0.504
0.963GluHis: 0.963 ± 0.294
3.186GluIle: 3.186 ± 0.458
3.705GluLys: 3.705 ± 0.641
4.52GluLeu: 4.52 ± 0.644
2.297GluMet: 2.297 ± 0.458
2.815GluAsn: 2.815 ± 0.37
1.482GluPro: 1.482 ± 0.392
2.297GluGln: 2.297 ± 0.536
3.705GluArg: 3.705 ± 0.565
2.297GluSer: 2.297 ± 0.375
2.964GluThr: 2.964 ± 0.478
4.668GluVal: 4.668 ± 0.682
1.778GluTrp: 1.778 ± 0.414
2.0GluTyr: 2.0 ± 0.441
0.0GluXaa: 0.0 ± 0.0
Phe
4.223PheAla: 4.223 ± 0.572
0.445PheCys: 0.445 ± 0.142
3.26PheAsp: 3.26 ± 0.502
2.075PheGlu: 2.075 ± 0.305
1.185PhePhe: 1.185 ± 0.428
3.186PheGly: 3.186 ± 0.721
0.519PheHis: 0.519 ± 0.19
1.926PheIle: 1.926 ± 0.399
2.223PheLys: 2.223 ± 0.494
1.482PheLeu: 1.482 ± 0.395
0.519PheMet: 0.519 ± 0.195
2.149PheAsn: 2.149 ± 0.42
1.334PhePro: 1.334 ± 0.304
0.963PheGln: 0.963 ± 0.227
1.63PheArg: 1.63 ± 0.364
2.075PheSer: 2.075 ± 0.379
3.779PheThr: 3.779 ± 0.614
3.26PheVal: 3.26 ± 0.443
0.445PheTrp: 0.445 ± 0.195
1.037PheTyr: 1.037 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
6.52GlyAla: 6.52 ± 0.679
1.185GlyCys: 1.185 ± 0.312
4.668GlyAsp: 4.668 ± 0.703
4.297GlyGlu: 4.297 ± 0.577
2.593GlyPhe: 2.593 ± 0.439
6.742GlyGly: 6.742 ± 0.92
1.111GlyHis: 1.111 ± 0.317
3.705GlyIle: 3.705 ± 0.404
6.001GlyLys: 6.001 ± 0.99
4.816GlyLeu: 4.816 ± 0.578
2.741GlyMet: 2.741 ± 0.501
3.927GlyAsn: 3.927 ± 0.668
2.075GlyPro: 2.075 ± 0.33
2.89GlyGln: 2.89 ± 0.499
4.89GlyArg: 4.89 ± 0.662
3.334GlySer: 3.334 ± 0.588
4.371GlyThr: 4.371 ± 0.868
6.372GlyVal: 6.372 ± 0.801
0.963GlyTrp: 0.963 ± 0.441
2.667GlyTyr: 2.667 ± 0.377
0.0GlyXaa: 0.0 ± 0.0
His
1.556HisAla: 1.556 ± 0.369
0.296HisCys: 0.296 ± 0.15
1.111HisAsp: 1.111 ± 0.332
1.111HisGlu: 1.111 ± 0.366
0.741HisPhe: 0.741 ± 0.236
0.815HisGly: 0.815 ± 0.266
0.148HisHis: 0.148 ± 0.122
0.889HisIle: 0.889 ± 0.229
0.741HisLys: 0.741 ± 0.213
1.556HisLeu: 1.556 ± 0.334
0.445HisMet: 0.445 ± 0.195
0.519HisAsn: 0.519 ± 0.183
1.111HisPro: 1.111 ± 0.25
0.593HisGln: 0.593 ± 0.239
1.037HisArg: 1.037 ± 0.242
0.741HisSer: 0.741 ± 0.213
0.889HisThr: 0.889 ± 0.277
1.037HisVal: 1.037 ± 0.274
0.222HisTrp: 0.222 ± 0.117
0.37HisTyr: 0.37 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
5.705IleAla: 5.705 ± 0.64
0.963IleCys: 0.963 ± 0.221
3.186IleAsp: 3.186 ± 0.493
3.705IleGlu: 3.705 ± 0.534
0.741IlePhe: 0.741 ± 0.234
4.445IleGly: 4.445 ± 0.639
0.445IleHis: 0.445 ± 0.264
3.556IleIle: 3.556 ± 0.595
3.112IleLys: 3.112 ± 0.503
3.779IleLeu: 3.779 ± 0.455
0.963IleMet: 0.963 ± 0.337
3.26IleAsn: 3.26 ± 0.632
2.445IlePro: 2.445 ± 0.407
1.334IleGln: 1.334 ± 0.318
2.593IleArg: 2.593 ± 0.515
2.593IleSer: 2.593 ± 0.404
3.705IleThr: 3.705 ± 0.587
3.186IleVal: 3.186 ± 0.626
0.741IleTrp: 0.741 ± 0.276
1.111IleTyr: 1.111 ± 0.384
0.0IleXaa: 0.0 ± 0.0
Lys
6.446LysAla: 6.446 ± 1.121
0.593LysCys: 0.593 ± 0.215
2.593LysAsp: 2.593 ± 0.5
2.815LysGlu: 2.815 ± 0.47
2.593LysPhe: 2.593 ± 0.434
3.408LysGly: 3.408 ± 0.708
1.185LysHis: 1.185 ± 0.26
2.593LysIle: 2.593 ± 0.397
4.297LysLys: 4.297 ± 0.996
4.742LysLeu: 4.742 ± 0.678
2.075LysMet: 2.075 ± 0.418
2.223LysAsn: 2.223 ± 0.415
2.89LysPro: 2.89 ± 0.531
3.334LysGln: 3.334 ± 0.542
4.001LysArg: 4.001 ± 0.701
3.186LysSer: 3.186 ± 0.653
3.334LysThr: 3.334 ± 0.515
4.149LysVal: 4.149 ± 0.566
0.889LysTrp: 0.889 ± 0.223
2.371LysTyr: 2.371 ± 0.415
0.0LysXaa: 0.0 ± 0.0
Leu
8.224LeuAla: 8.224 ± 0.805
0.963LeuCys: 0.963 ± 0.222
4.371LeuAsp: 4.371 ± 0.579
5.26LeuGlu: 5.26 ± 0.592
2.149LeuPhe: 2.149 ± 0.344
4.816LeuGly: 4.816 ± 0.467
0.519LeuHis: 0.519 ± 0.209
2.964LeuIle: 2.964 ± 0.478
2.89LeuLys: 2.89 ± 0.55
5.335LeuLeu: 5.335 ± 0.701
1.926LeuMet: 1.926 ± 0.341
4.001LeuAsn: 4.001 ± 0.473
4.149LeuPro: 4.149 ± 0.596
3.705LeuGln: 3.705 ± 0.457
4.816LeuArg: 4.816 ± 0.649
4.371LeuSer: 4.371 ± 0.471
4.075LeuThr: 4.075 ± 0.458
5.038LeuVal: 5.038 ± 0.713
1.037LeuTrp: 1.037 ± 0.238
1.852LeuTyr: 1.852 ± 0.367
0.0LeuXaa: 0.0 ± 0.0
Met
2.371MetAla: 2.371 ± 0.364
0.37MetCys: 0.37 ± 0.193
1.185MetAsp: 1.185 ± 0.266
1.26MetGlu: 1.26 ± 0.225
1.63MetPhe: 1.63 ± 0.363
1.482MetGly: 1.482 ± 0.284
0.889MetHis: 0.889 ± 0.26
1.482MetIle: 1.482 ± 0.391
1.852MetLys: 1.852 ± 0.418
1.926MetLeu: 1.926 ± 0.394
0.889MetMet: 0.889 ± 0.222
1.408MetAsn: 1.408 ± 0.33
1.704MetPro: 1.704 ± 0.332
1.704MetGln: 1.704 ± 0.37
2.075MetArg: 2.075 ± 0.344
1.852MetSer: 1.852 ± 0.418
1.482MetThr: 1.482 ± 0.269
1.482MetVal: 1.482 ± 0.299
0.519MetTrp: 0.519 ± 0.153
1.111MetTyr: 1.111 ± 0.213
0.0MetXaa: 0.0 ± 0.0
Asn
5.483AsnAla: 5.483 ± 0.88
0.296AsnCys: 0.296 ± 0.146
2.964AsnAsp: 2.964 ± 0.437
1.704AsnGlu: 1.704 ± 0.432
1.63AsnPhe: 1.63 ± 0.355
4.668AsnGly: 4.668 ± 0.782
0.667AsnHis: 0.667 ± 0.252
2.371AsnIle: 2.371 ± 0.408
2.593AsnLys: 2.593 ± 0.393
3.408AsnLeu: 3.408 ± 0.487
1.556AsnMet: 1.556 ± 0.328
2.297AsnAsn: 2.297 ± 0.525
2.667AsnPro: 2.667 ± 0.382
1.26AsnGln: 1.26 ± 0.338
1.556AsnArg: 1.556 ± 0.322
1.704AsnSer: 1.704 ± 0.335
2.0AsnThr: 2.0 ± 0.384
4.297AsnVal: 4.297 ± 0.611
0.815AsnTrp: 0.815 ± 0.226
1.704AsnTyr: 1.704 ± 0.338
0.0AsnXaa: 0.0 ± 0.0
Pro
4.223ProAla: 4.223 ± 0.639
0.074ProCys: 0.074 ± 0.076
2.964ProAsp: 2.964 ± 0.436
2.371ProGlu: 2.371 ± 0.593
1.926ProPhe: 1.926 ± 0.466
3.556ProGly: 3.556 ± 0.423
0.815ProHis: 0.815 ± 0.316
1.926ProIle: 1.926 ± 0.293
3.408ProLys: 3.408 ± 0.548
2.593ProLeu: 2.593 ± 0.389
0.815ProMet: 0.815 ± 0.229
2.075ProAsn: 2.075 ± 0.534
1.704ProPro: 1.704 ± 0.471
1.556ProGln: 1.556 ± 0.314
2.593ProArg: 2.593 ± 0.484
3.112ProSer: 3.112 ± 0.38
1.926ProThr: 1.926 ± 0.368
3.556ProVal: 3.556 ± 0.534
0.815ProTrp: 0.815 ± 0.243
0.963ProTyr: 0.963 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
4.075GlnAla: 4.075 ± 0.691
0.741GlnCys: 0.741 ± 0.213
2.0GlnAsp: 2.0 ± 0.403
1.26GlnGlu: 1.26 ± 0.414
1.704GlnPhe: 1.704 ± 0.291
2.741GlnGly: 2.741 ± 0.499
0.667GlnHis: 0.667 ± 0.224
2.297GlnIle: 2.297 ± 0.37
1.852GlnLys: 1.852 ± 0.412
3.779GlnLeu: 3.779 ± 0.481
1.334GlnMet: 1.334 ± 0.278
1.704GlnAsn: 1.704 ± 0.343
2.075GlnPro: 2.075 ± 0.395
2.0GlnGln: 2.0 ± 0.386
2.519GlnArg: 2.519 ± 0.523
1.704GlnSer: 1.704 ± 0.367
2.445GlnThr: 2.445 ± 0.408
2.297GlnVal: 2.297 ± 0.363
0.593GlnTrp: 0.593 ± 0.212
1.185GlnTyr: 1.185 ± 0.268
0.0GlnXaa: 0.0 ± 0.0
Arg
5.409ArgAla: 5.409 ± 0.53
0.445ArgCys: 0.445 ± 0.171
4.223ArgAsp: 4.223 ± 0.733
3.556ArgGlu: 3.556 ± 0.597
1.926ArgPhe: 1.926 ± 0.353
4.445ArgGly: 4.445 ± 0.688
1.334ArgHis: 1.334 ± 0.354
4.075ArgIle: 4.075 ± 0.55
3.482ArgLys: 3.482 ± 0.428
5.483ArgLeu: 5.483 ± 0.584
1.926ArgMet: 1.926 ± 0.336
2.371ArgAsn: 2.371 ± 0.421
2.075ArgPro: 2.075 ± 0.417
1.63ArgGln: 1.63 ± 0.351
4.89ArgArg: 4.89 ± 1.055
2.297ArgSer: 2.297 ± 0.398
3.26ArgThr: 3.26 ± 0.484
5.483ArgVal: 5.483 ± 0.593
1.334ArgTrp: 1.334 ± 0.327
2.149ArgTyr: 2.149 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
5.631SerAla: 5.631 ± 0.585
0.667SerCys: 0.667 ± 0.28
2.815SerAsp: 2.815 ± 0.439
3.112SerGlu: 3.112 ± 0.416
1.482SerPhe: 1.482 ± 0.286
5.335SerGly: 5.335 ± 0.617
1.037SerHis: 1.037 ± 0.247
2.445SerIle: 2.445 ± 0.511
3.556SerLys: 3.556 ± 0.411
3.408SerLeu: 3.408 ± 0.53
1.482SerMet: 1.482 ± 0.297
2.223SerAsn: 2.223 ± 0.378
2.297SerPro: 2.297 ± 0.462
1.926SerGln: 1.926 ± 0.431
3.482SerArg: 3.482 ± 0.446
2.741SerSer: 2.741 ± 0.512
3.482SerThr: 3.482 ± 0.721
3.112SerVal: 3.112 ± 0.405
0.667SerTrp: 0.667 ± 0.196
1.26SerTyr: 1.26 ± 0.386
0.0SerXaa: 0.0 ± 0.0
Thr
7.039ThrAla: 7.039 ± 0.815
0.815ThrCys: 0.815 ± 0.256
3.112ThrAsp: 3.112 ± 0.512
3.26ThrGlu: 3.26 ± 0.417
2.815ThrPhe: 2.815 ± 0.572
6.298ThrGly: 6.298 ± 0.908
0.37ThrHis: 0.37 ± 0.159
3.556ThrIle: 3.556 ± 0.551
2.371ThrLys: 2.371 ± 0.402
4.594ThrLeu: 4.594 ± 0.7
1.704ThrMet: 1.704 ± 0.282
2.075ThrAsn: 2.075 ± 0.47
3.112ThrPro: 3.112 ± 0.57
2.223ThrGln: 2.223 ± 0.46
2.741ThrArg: 2.741 ± 0.404
3.038ThrSer: 3.038 ± 0.48
3.482ThrThr: 3.482 ± 0.793
4.223ThrVal: 4.223 ± 0.832
0.667ThrTrp: 0.667 ± 0.18
1.26ThrTyr: 1.26 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
7.113ValAla: 7.113 ± 0.87
0.889ValCys: 0.889 ± 0.275
4.816ValAsp: 4.816 ± 0.619
4.223ValGlu: 4.223 ± 0.722
2.075ValPhe: 2.075 ± 0.348
4.89ValGly: 4.89 ± 0.415
1.63ValHis: 1.63 ± 0.354
3.779ValIle: 3.779 ± 0.454
4.445ValLys: 4.445 ± 0.584
5.409ValLeu: 5.409 ± 0.54
2.0ValMet: 2.0 ± 0.392
2.815ValAsn: 2.815 ± 0.521
2.741ValPro: 2.741 ± 0.522
2.667ValGln: 2.667 ± 0.495
5.409ValArg: 5.409 ± 0.643
5.335ValSer: 5.335 ± 0.822
4.668ValThr: 4.668 ± 0.699
5.186ValVal: 5.186 ± 0.723
1.26ValTrp: 1.26 ± 0.311
2.445ValTyr: 2.445 ± 0.342
0.0ValXaa: 0.0 ± 0.0
Trp
1.185TrpAla: 1.185 ± 0.282
0.296TrpCys: 0.296 ± 0.121
0.963TrpAsp: 0.963 ± 0.227
0.593TrpGlu: 0.593 ± 0.206
0.445TrpPhe: 0.445 ± 0.22
1.037TrpGly: 1.037 ± 0.34
0.222TrpHis: 0.222 ± 0.12
0.445TrpIle: 0.445 ± 0.157
1.334TrpLys: 1.334 ± 0.37
1.63TrpLeu: 1.63 ± 0.334
0.445TrpMet: 0.445 ± 0.145
0.963TrpAsn: 0.963 ± 0.237
0.815TrpPro: 0.815 ± 0.229
1.26TrpGln: 1.26 ± 0.315
1.556TrpArg: 1.556 ± 0.337
0.815TrpSer: 0.815 ± 0.279
0.741TrpThr: 0.741 ± 0.26
1.111TrpVal: 1.111 ± 0.307
0.222TrpTrp: 0.222 ± 0.115
0.222TrpTyr: 0.222 ± 0.12
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.038TyrAla: 3.038 ± 0.44
0.148TyrCys: 0.148 ± 0.105
1.556TyrAsp: 1.556 ± 0.404
1.111TyrGlu: 1.111 ± 0.332
1.778TyrPhe: 1.778 ± 0.276
2.0TyrGly: 2.0 ± 0.311
0.667TyrHis: 0.667 ± 0.2
1.334TyrIle: 1.334 ± 0.359
1.334TyrLys: 1.334 ± 0.353
1.852TyrLeu: 1.852 ± 0.333
0.889TyrMet: 0.889 ± 0.233
1.778TyrAsn: 1.778 ± 0.336
1.704TyrPro: 1.704 ± 0.391
1.26TyrGln: 1.26 ± 0.295
2.223TyrArg: 2.223 ± 0.389
2.223TyrSer: 2.223 ± 0.417
2.223TyrThr: 2.223 ± 0.359
2.075TyrVal: 2.075 ± 0.397
0.445TyrTrp: 0.445 ± 0.199
1.26TyrTyr: 1.26 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13498 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski