Pseudomonas phage vB_Pae-SS2019XI

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XH26|A0A6G6XH26_9CAUD Capsid and scaffold protein OS=Pseudomonas phage vB_Pae-SS2019XI OX=2660688 GN=vBPaeSS2019XI_003 PE=4 SV=1
MM1 pKa = 8.03SIEE4 pKa = 4.65EE5 pKa = 4.07YY6 pKa = 10.76LKK8 pKa = 10.91SCLEE12 pKa = 4.83DD13 pKa = 4.04GLDD16 pKa = 3.46THH18 pKa = 7.05RR19 pKa = 11.84LRR21 pKa = 11.84VSLTVDD27 pKa = 3.57GKK29 pKa = 9.14VQFYY33 pKa = 9.71IHH35 pKa = 7.26PEE37 pKa = 4.11GEE39 pKa = 3.89EE40 pKa = 3.98EE41 pKa = 3.96EE42 pKa = 5.13AVDD45 pKa = 4.76FEE47 pKa = 5.09VDD49 pKa = 3.37GSTLIHH55 pKa = 5.72VTDD58 pKa = 3.81

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XGQ2|A0A6G6XGQ2_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_Pae-SS2019XI OX=2660688 GN=vBPaeSS2019XI_042 PE=4 SV=1
MM1 pKa = 7.28TRR3 pKa = 11.84QPHH6 pKa = 4.9YY7 pKa = 11.0VVVTGGRR14 pKa = 11.84YY15 pKa = 6.51FTNKK19 pKa = 8.78EE20 pKa = 4.11VIYY23 pKa = 10.19GALNAVLSEE32 pKa = 3.79FRR34 pKa = 11.84KK35 pKa = 10.1SNKK38 pKa = 9.35AGTRR42 pKa = 11.84RR43 pKa = 11.84FVLVHH48 pKa = 6.01GAAPGADD55 pKa = 3.01TLSARR60 pKa = 11.84WARR63 pKa = 11.84DD64 pKa = 3.16CGLAALAVPAEE75 pKa = 3.93WMTFGRR81 pKa = 11.84SAGPRR86 pKa = 11.84RR87 pKa = 11.84NAEE90 pKa = 3.81MLEE93 pKa = 4.12WVPAKK98 pKa = 10.71LLVAFPGGRR107 pKa = 11.84GTAHH111 pKa = 5.7MVALARR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84GLPVRR124 pKa = 11.84QVHH127 pKa = 6.06IPSSDD132 pKa = 3.39DD133 pKa = 3.98DD134 pKa = 3.89EE135 pKa = 6.11FEE137 pKa = 4.08VARR140 pKa = 11.84ASLQKK145 pKa = 10.44IGGAPP150 pKa = 3.14

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

18015

34

1415

217.0

24.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.096 ± 0.556

1.16 ± 0.138

6.173 ± 0.237

6.556 ± 0.272

3.497 ± 0.118

7.366 ± 0.25

1.865 ± 0.17

4.235 ± 0.175

4.635 ± 0.273

8.909 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.128

3.547 ± 0.207

4.829 ± 0.306

3.969 ± 0.329

7.227 ± 0.279

5.556 ± 0.212

5.329 ± 0.277

6.978 ± 0.219

1.81 ± 0.174

2.992 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski