Amino acid dipepetide frequency for Bacillus phage BCJA1c

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.362AlaAla: 5.362 ± 1.518
0.394AlaCys: 0.394 ± 0.179
4.179AlaAsp: 4.179 ± 0.693
6.388AlaGlu: 6.388 ± 0.807
2.366AlaPhe: 2.366 ± 0.433
4.022AlaGly: 4.022 ± 0.618
1.498AlaHis: 1.498 ± 0.371
4.495AlaIle: 4.495 ± 0.895
6.545AlaLys: 6.545 ± 0.601
5.993AlaLeu: 5.993 ± 0.751
1.814AlaMet: 1.814 ± 0.412
3.312AlaAsn: 3.312 ± 0.418
1.183AlaPro: 1.183 ± 0.245
3.864AlaGln: 3.864 ± 0.769
3.075AlaArg: 3.075 ± 0.528
3.785AlaSer: 3.785 ± 0.75
3.706AlaThr: 3.706 ± 0.625
4.495AlaVal: 4.495 ± 0.833
0.71AlaTrp: 0.71 ± 0.26
2.129AlaTyr: 2.129 ± 0.484
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.135
0.0CysCys: 0.0 ± 0.0
0.552CysAsp: 0.552 ± 0.267
0.473CysGlu: 0.473 ± 0.163
0.237CysPhe: 0.237 ± 0.127
0.71CysGly: 0.71 ± 0.289
0.237CysHis: 0.237 ± 0.152
0.079CysIle: 0.079 ± 0.076
0.473CysLys: 0.473 ± 0.18
0.473CysLeu: 0.473 ± 0.212
0.079CysMet: 0.079 ± 0.063
0.552CysAsn: 0.552 ± 0.227
0.552CysPro: 0.552 ± 0.263
0.079CysGln: 0.079 ± 0.08
0.158CysArg: 0.158 ± 0.098
0.946CysSer: 0.946 ± 0.328
0.237CysThr: 0.237 ± 0.142
0.552CysVal: 0.552 ± 0.257
0.079CysTrp: 0.079 ± 0.067
0.158CysTyr: 0.158 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
3.864AspAla: 3.864 ± 0.48
0.631AspCys: 0.631 ± 0.233
3.864AspAsp: 3.864 ± 0.752
6.309AspGlu: 6.309 ± 0.62
2.839AspPhe: 2.839 ± 0.571
5.205AspGly: 5.205 ± 0.669
2.05AspHis: 2.05 ± 0.366
4.495AspIle: 4.495 ± 0.65
4.889AspLys: 4.889 ± 0.563
5.993AspLeu: 5.993 ± 0.667
1.735AspMet: 1.735 ± 0.302
2.918AspAsn: 2.918 ± 0.399
2.287AspPro: 2.287 ± 0.452
1.893AspGln: 1.893 ± 0.371
3.627AspArg: 3.627 ± 0.762
2.76AspSer: 2.76 ± 0.449
2.287AspThr: 2.287 ± 0.421
3.391AspVal: 3.391 ± 0.465
0.946AspTrp: 0.946 ± 0.272
3.233AspTyr: 3.233 ± 0.649
0.0AspXaa: 0.0 ± 0.0
Glu
5.047GluAla: 5.047 ± 0.592
0.552GluCys: 0.552 ± 0.237
5.757GluAsp: 5.757 ± 0.713
7.965GluGlu: 7.965 ± 0.953
3.549GluPhe: 3.549 ± 0.673
5.205GluGly: 5.205 ± 0.723
1.814GluHis: 1.814 ± 0.332
6.072GluIle: 6.072 ± 0.659
6.151GluLys: 6.151 ± 1.008
9.069GluLeu: 9.069 ± 0.829
2.602GluMet: 2.602 ± 0.504
4.416GluAsn: 4.416 ± 0.639
2.287GluPro: 2.287 ± 0.478
3.47GluGln: 3.47 ± 0.602
4.653GluArg: 4.653 ± 0.673
5.126GluSer: 5.126 ± 0.733
3.391GluThr: 3.391 ± 0.465
4.258GluVal: 4.258 ± 0.706
1.419GluTrp: 1.419 ± 0.263
4.179GluTyr: 4.179 ± 0.653
0.0GluXaa: 0.0 ± 0.0
Phe
2.681PheAla: 2.681 ± 0.354
0.315PheCys: 0.315 ± 0.156
3.391PheAsp: 3.391 ± 0.433
3.075PheGlu: 3.075 ± 0.539
0.946PhePhe: 0.946 ± 0.26
2.208PheGly: 2.208 ± 0.355
0.552PheHis: 0.552 ± 0.197
2.445PheIle: 2.445 ± 0.495
2.997PheLys: 2.997 ± 0.502
2.129PheLeu: 2.129 ± 0.483
1.341PheMet: 1.341 ± 0.33
2.839PheAsn: 2.839 ± 0.354
0.789PhePro: 0.789 ± 0.266
1.419PheGln: 1.419 ± 0.391
1.893PheArg: 1.893 ± 0.497
1.656PheSer: 1.656 ± 0.451
1.498PheThr: 1.498 ± 0.352
2.445PheVal: 2.445 ± 0.43
0.631PheTrp: 0.631 ± 0.22
1.735PheTyr: 1.735 ± 0.508
0.0PheXaa: 0.0 ± 0.0
Gly
4.022GlyAla: 4.022 ± 0.887
0.394GlyCys: 0.394 ± 0.238
2.997GlyAsp: 2.997 ± 0.479
5.362GlyGlu: 5.362 ± 0.621
2.602GlyPhe: 2.602 ± 0.45
3.233GlyGly: 3.233 ± 0.413
1.262GlyHis: 1.262 ± 0.276
3.864GlyIle: 3.864 ± 0.554
4.968GlyLys: 4.968 ± 0.812
5.205GlyLeu: 5.205 ± 0.606
1.814GlyMet: 1.814 ± 0.429
3.154GlyAsn: 3.154 ± 0.355
1.735GlyPro: 1.735 ± 0.293
3.391GlyGln: 3.391 ± 0.675
3.391GlyArg: 3.391 ± 0.58
3.391GlySer: 3.391 ± 0.59
3.47GlyThr: 3.47 ± 0.808
4.81GlyVal: 4.81 ± 0.582
1.025GlyTrp: 1.025 ± 0.35
2.602GlyTyr: 2.602 ± 0.552
0.0GlyXaa: 0.0 ± 0.0
His
1.341HisAla: 1.341 ± 0.346
0.237HisCys: 0.237 ± 0.14
1.104HisAsp: 1.104 ± 0.335
1.814HisGlu: 1.814 ± 0.376
1.025HisPhe: 1.025 ± 0.27
1.498HisGly: 1.498 ± 0.288
0.946HisHis: 0.946 ± 0.231
1.419HisIle: 1.419 ± 0.378
1.183HisLys: 1.183 ± 0.319
1.025HisLeu: 1.025 ± 0.283
0.394HisMet: 0.394 ± 0.139
0.789HisAsn: 0.789 ± 0.232
0.867HisPro: 0.867 ± 0.297
0.473HisGln: 0.473 ± 0.199
1.341HisArg: 1.341 ± 0.37
0.867HisSer: 0.867 ± 0.246
1.577HisThr: 1.577 ± 0.393
1.025HisVal: 1.025 ± 0.311
0.237HisTrp: 0.237 ± 0.141
0.867HisTyr: 0.867 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
5.283IleAla: 5.283 ± 0.613
0.473IleCys: 0.473 ± 0.178
5.993IleAsp: 5.993 ± 0.56
6.072IleGlu: 6.072 ± 0.591
1.419IlePhe: 1.419 ± 0.392
2.918IleGly: 2.918 ± 0.561
1.025IleHis: 1.025 ± 0.252
3.943IleIle: 3.943 ± 0.655
4.495IleLys: 4.495 ± 0.627
3.075IleLeu: 3.075 ± 0.63
1.025IleMet: 1.025 ± 0.293
4.179IleAsn: 4.179 ± 0.546
1.971IlePro: 1.971 ± 0.404
3.075IleGln: 3.075 ± 0.432
3.47IleArg: 3.47 ± 0.593
4.022IleSer: 4.022 ± 0.731
4.495IleThr: 4.495 ± 0.575
2.918IleVal: 2.918 ± 0.385
0.394IleTrp: 0.394 ± 0.169
2.208IleTyr: 2.208 ± 0.394
0.0IleXaa: 0.0 ± 0.0
Lys
6.309LysAla: 6.309 ± 0.829
0.631LysCys: 0.631 ± 0.324
4.574LysAsp: 4.574 ± 0.563
7.334LysGlu: 7.334 ± 1.058
1.577LysPhe: 1.577 ± 0.333
4.258LysGly: 4.258 ± 0.552
1.735LysHis: 1.735 ± 0.366
4.653LysIle: 4.653 ± 0.61
7.965LysLys: 7.965 ± 1.362
5.836LysLeu: 5.836 ± 0.941
2.445LysMet: 2.445 ± 0.551
3.549LysAsn: 3.549 ± 0.622
2.445LysPro: 2.445 ± 0.489
3.943LysGln: 3.943 ± 0.546
3.391LysArg: 3.391 ± 0.505
4.416LysSer: 4.416 ± 0.784
4.101LysThr: 4.101 ± 0.858
5.599LysVal: 5.599 ± 0.534
1.025LysTrp: 1.025 ± 0.267
2.76LysTyr: 2.76 ± 0.605
0.0LysXaa: 0.0 ± 0.0
Leu
4.574LeuAla: 4.574 ± 0.664
0.552LeuCys: 0.552 ± 0.24
6.151LeuAsp: 6.151 ± 0.591
7.965LeuGlu: 7.965 ± 1.039
2.366LeuPhe: 2.366 ± 0.305
5.441LeuGly: 5.441 ± 0.5
1.656LeuHis: 1.656 ± 0.376
4.101LeuIle: 4.101 ± 0.637
7.413LeuLys: 7.413 ± 1.226
5.441LeuLeu: 5.441 ± 0.959
1.419LeuMet: 1.419 ± 0.318
4.574LeuAsn: 4.574 ± 0.537
2.918LeuPro: 2.918 ± 0.455
3.706LeuGln: 3.706 ± 0.657
5.047LeuArg: 5.047 ± 0.652
5.047LeuSer: 5.047 ± 0.669
3.943LeuThr: 3.943 ± 0.342
3.785LeuVal: 3.785 ± 0.454
0.473LeuTrp: 0.473 ± 0.182
2.839LeuTyr: 2.839 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
2.129MetAla: 2.129 ± 0.554
0.237MetCys: 0.237 ± 0.144
2.129MetAsp: 2.129 ± 0.435
2.129MetGlu: 2.129 ± 0.442
1.025MetPhe: 1.025 ± 0.24
1.183MetGly: 1.183 ± 0.293
0.552MetHis: 0.552 ± 0.231
1.419MetIle: 1.419 ± 0.359
2.05MetLys: 2.05 ± 0.459
1.893MetLeu: 1.893 ± 0.356
0.473MetMet: 0.473 ± 0.217
1.419MetAsn: 1.419 ± 0.317
1.341MetPro: 1.341 ± 0.325
1.262MetGln: 1.262 ± 0.334
1.577MetArg: 1.577 ± 0.366
1.656MetSer: 1.656 ± 0.419
1.262MetThr: 1.262 ± 0.338
1.419MetVal: 1.419 ± 0.341
0.473MetTrp: 0.473 ± 0.173
0.473MetTyr: 0.473 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
3.943AsnAla: 3.943 ± 0.589
0.315AsnCys: 0.315 ± 0.169
3.706AsnAsp: 3.706 ± 0.549
3.233AsnGlu: 3.233 ± 0.374
1.341AsnPhe: 1.341 ± 0.35
3.706AsnGly: 3.706 ± 0.544
1.104AsnHis: 1.104 ± 0.3
3.233AsnIle: 3.233 ± 0.499
3.627AsnLys: 3.627 ± 0.484
4.022AsnLeu: 4.022 ± 0.551
1.577AsnMet: 1.577 ± 0.317
3.075AsnAsn: 3.075 ± 0.629
1.498AsnPro: 1.498 ± 0.431
2.681AsnGln: 2.681 ± 0.439
2.76AsnArg: 2.76 ± 0.459
3.864AsnSer: 3.864 ± 0.513
2.918AsnThr: 2.918 ± 0.605
3.154AsnVal: 3.154 ± 0.406
1.025AsnTrp: 1.025 ± 0.337
1.419AsnTyr: 1.419 ± 0.378
0.0AsnXaa: 0.0 ± 0.0
Pro
1.735ProAla: 1.735 ± 0.495
0.237ProCys: 0.237 ± 0.135
1.971ProAsp: 1.971 ± 0.436
3.391ProGlu: 3.391 ± 0.577
1.419ProPhe: 1.419 ± 0.36
2.602ProGly: 2.602 ± 0.527
0.631ProHis: 0.631 ± 0.186
1.971ProIle: 1.971 ± 0.429
2.05ProLys: 2.05 ± 0.479
2.839ProLeu: 2.839 ± 0.478
0.394ProMet: 0.394 ± 0.166
1.419ProAsn: 1.419 ± 0.327
1.262ProPro: 1.262 ± 0.338
1.419ProGln: 1.419 ± 0.353
1.025ProArg: 1.025 ± 0.285
2.05ProSer: 2.05 ± 0.376
1.577ProThr: 1.577 ± 0.281
2.05ProVal: 2.05 ± 0.408
0.315ProTrp: 0.315 ± 0.143
1.656ProTyr: 1.656 ± 0.381
0.0ProXaa: 0.0 ± 0.0
Gln
4.731GlnAla: 4.731 ± 0.598
0.158GlnCys: 0.158 ± 0.115
1.735GlnAsp: 1.735 ± 0.278
3.785GlnGlu: 3.785 ± 0.574
1.893GlnPhe: 1.893 ± 0.372
2.523GlnGly: 2.523 ± 0.537
0.946GlnHis: 0.946 ± 0.272
3.312GlnIle: 3.312 ± 0.582
3.943GlnLys: 3.943 ± 0.639
3.549GlnLeu: 3.549 ± 0.573
1.735GlnMet: 1.735 ± 0.481
2.366GlnAsn: 2.366 ± 0.499
1.656GlnPro: 1.656 ± 0.511
3.549GlnGln: 3.549 ± 0.751
2.287GlnArg: 2.287 ± 0.451
1.419GlnSer: 1.419 ± 0.268
1.971GlnThr: 1.971 ± 0.392
2.523GlnVal: 2.523 ± 0.488
0.552GlnTrp: 0.552 ± 0.219
1.498GlnTyr: 1.498 ± 0.291
0.0GlnXaa: 0.0 ± 0.0
Arg
2.681ArgAla: 2.681 ± 0.473
0.473ArgCys: 0.473 ± 0.219
2.76ArgAsp: 2.76 ± 0.365
3.706ArgGlu: 3.706 ± 0.539
2.445ArgPhe: 2.445 ± 0.401
2.523ArgGly: 2.523 ± 0.379
0.71ArgHis: 0.71 ± 0.284
3.785ArgIle: 3.785 ± 0.593
4.889ArgLys: 4.889 ± 0.874
4.416ArgLeu: 4.416 ± 0.53
1.735ArgMet: 1.735 ± 0.423
2.287ArgAsn: 2.287 ± 0.505
1.262ArgPro: 1.262 ± 0.308
2.602ArgGln: 2.602 ± 0.569
2.602ArgArg: 2.602 ± 0.438
2.839ArgSer: 2.839 ± 0.501
3.627ArgThr: 3.627 ± 0.489
4.179ArgVal: 4.179 ± 0.587
0.158ArgTrp: 0.158 ± 0.107
1.735ArgTyr: 1.735 ± 0.298
0.0ArgXaa: 0.0 ± 0.0
Ser
3.706SerAla: 3.706 ± 0.547
0.473SerCys: 0.473 ± 0.214
3.627SerAsp: 3.627 ± 0.414
4.337SerGlu: 4.337 ± 0.539
2.76SerPhe: 2.76 ± 0.4
4.574SerGly: 4.574 ± 0.761
0.552SerHis: 0.552 ± 0.225
3.785SerIle: 3.785 ± 0.464
3.312SerLys: 3.312 ± 0.4
4.495SerLeu: 4.495 ± 0.67
1.577SerMet: 1.577 ± 0.412
2.681SerAsn: 2.681 ± 0.423
1.893SerPro: 1.893 ± 0.348
2.997SerGln: 2.997 ± 0.409
2.76SerArg: 2.76 ± 0.445
3.706SerSer: 3.706 ± 0.567
3.627SerThr: 3.627 ± 0.711
3.706SerVal: 3.706 ± 0.458
0.946SerTrp: 0.946 ± 0.326
2.287SerTyr: 2.287 ± 0.419
0.0SerXaa: 0.0 ± 0.0
Thr
4.81ThrAla: 4.81 ± 0.822
0.237ThrCys: 0.237 ± 0.142
2.366ThrAsp: 2.366 ± 0.385
3.864ThrGlu: 3.864 ± 0.461
2.445ThrPhe: 2.445 ± 0.449
4.337ThrGly: 4.337 ± 0.592
1.025ThrHis: 1.025 ± 0.356
2.997ThrIle: 2.997 ± 0.507
3.785ThrLys: 3.785 ± 0.743
5.441ThrLeu: 5.441 ± 0.669
0.71ThrMet: 0.71 ± 0.296
2.129ThrAsn: 2.129 ± 0.294
2.997ThrPro: 2.997 ± 0.48
1.814ThrGln: 1.814 ± 0.425
2.129ThrArg: 2.129 ± 0.378
3.47ThrSer: 3.47 ± 0.659
3.391ThrThr: 3.391 ± 0.594
3.706ThrVal: 3.706 ± 0.537
0.946ThrTrp: 0.946 ± 0.261
1.735ThrTyr: 1.735 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
4.179ValAla: 4.179 ± 0.729
0.315ValCys: 0.315 ± 0.122
4.731ValAsp: 4.731 ± 0.823
4.81ValGlu: 4.81 ± 0.579
2.602ValPhe: 2.602 ± 0.5
4.179ValGly: 4.179 ± 0.633
0.71ValHis: 0.71 ± 0.252
3.864ValIle: 3.864 ± 0.59
4.81ValLys: 4.81 ± 0.66
4.416ValLeu: 4.416 ± 0.744
1.735ValMet: 1.735 ± 0.424
3.47ValAsn: 3.47 ± 0.523
1.735ValPro: 1.735 ± 0.328
1.735ValGln: 1.735 ± 0.369
3.47ValArg: 3.47 ± 0.652
4.101ValSer: 4.101 ± 0.531
3.706ValThr: 3.706 ± 0.457
4.022ValVal: 4.022 ± 0.524
0.394ValTrp: 0.394 ± 0.235
2.287ValTyr: 2.287 ± 0.457
0.0ValXaa: 0.0 ± 0.0
Trp
0.789TrpAla: 0.789 ± 0.227
0.158TrpCys: 0.158 ± 0.106
1.498TrpAsp: 1.498 ± 0.374
1.183TrpGlu: 1.183 ± 0.355
0.394TrpPhe: 0.394 ± 0.142
0.473TrpGly: 0.473 ± 0.213
0.237TrpHis: 0.237 ± 0.14
0.552TrpIle: 0.552 ± 0.207
0.473TrpLys: 0.473 ± 0.205
1.104TrpLeu: 1.104 ± 0.232
0.394TrpMet: 0.394 ± 0.186
0.789TrpAsn: 0.789 ± 0.236
0.0TrpPro: 0.0 ± 0.0
0.789TrpGln: 0.789 ± 0.326
0.631TrpArg: 0.631 ± 0.148
1.025TrpSer: 1.025 ± 0.231
0.867TrpThr: 0.867 ± 0.263
0.71TrpVal: 0.71 ± 0.259
0.158TrpTrp: 0.158 ± 0.101
0.315TrpTyr: 0.315 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.893TyrAla: 1.893 ± 0.378
0.079TyrCys: 0.079 ± 0.069
2.129TyrAsp: 2.129 ± 0.469
3.785TyrGlu: 3.785 ± 0.631
1.735TyrPhe: 1.735 ± 0.388
1.893TyrGly: 1.893 ± 0.468
0.789TyrHis: 0.789 ± 0.182
1.971TyrIle: 1.971 ± 0.406
2.602TyrLys: 2.602 ± 0.568
2.997TyrLeu: 2.997 ± 0.611
1.025TyrMet: 1.025 ± 0.277
2.287TyrAsn: 2.287 ± 0.426
1.341TyrPro: 1.341 ± 0.301
1.971TyrGln: 1.971 ± 0.365
2.129TyrArg: 2.129 ± 0.382
1.656TyrSer: 1.656 ± 0.365
2.681TyrThr: 2.681 ± 0.377
2.523TyrVal: 2.523 ± 0.375
0.631TyrTrp: 0.631 ± 0.206
1.577TyrTyr: 1.577 ± 0.543
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12682 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski