Lake Sarah-associated circular molecule 10

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G8G1|A0A126G8G1_9VIRU Replication associated protein OS=Lake Sarah-associated circular molecule 10 OX=1685725 PE=4 SV=1
MM1 pKa = 6.78TTDD4 pKa = 5.19KK5 pKa = 11.24KK6 pKa = 10.43FTTQNNTKK14 pKa = 8.7YY15 pKa = 9.63WCFTWDD21 pKa = 3.38TNISQKK27 pKa = 10.69KK28 pKa = 8.72LPPVADD34 pKa = 5.41LINFLNNISEE44 pKa = 4.17TAIFQEE50 pKa = 4.19EE51 pKa = 4.91CGTIKK56 pKa = 10.84GKK58 pKa = 8.3VHH60 pKa = 5.26YY61 pKa = 10.15QGTLTLIGPRR71 pKa = 11.84QSKK74 pKa = 10.38ISVLRR79 pKa = 11.84LFEE82 pKa = 4.19RR83 pKa = 11.84RR84 pKa = 11.84FKK86 pKa = 10.96GVSGLTLSRR95 pKa = 11.84SYY97 pKa = 11.5SNQASEE103 pKa = 5.47DD104 pKa = 4.09YY105 pKa = 9.33VTKK108 pKa = 10.76DD109 pKa = 3.02EE110 pKa = 4.41GRR112 pKa = 11.84ISGPYY117 pKa = 9.45FCGKK121 pKa = 9.8KK122 pKa = 9.94EE123 pKa = 4.06KK124 pKa = 10.69FSMEE128 pKa = 3.86YY129 pKa = 9.37STTKK133 pKa = 9.16LTAWQQDD140 pKa = 3.55LFDD143 pKa = 5.22FLTKK147 pKa = 9.16ATKK150 pKa = 9.66IKK152 pKa = 10.14YY153 pKa = 9.05FRR155 pKa = 11.84DD156 pKa = 3.58RR157 pKa = 11.84IVIVVQDD164 pKa = 3.67EE165 pKa = 4.34KK166 pKa = 11.59GNSGKK171 pKa = 10.69SFFLKK176 pKa = 9.89WLAIGQKK183 pKa = 9.76VLNSKK188 pKa = 9.96KK189 pKa = 10.75LPVTSVDD196 pKa = 3.37RR197 pKa = 11.84LMSAVTKK204 pKa = 9.3VTADD208 pKa = 3.27SKK210 pKa = 11.2RR211 pKa = 11.84IDD213 pKa = 3.99LFTINLTRR221 pKa = 11.84SKK223 pKa = 11.42GEE225 pKa = 3.86DD226 pKa = 3.25QSYY229 pKa = 11.23KK230 pKa = 11.04DD231 pKa = 3.53LFAAIEE237 pKa = 4.33EE238 pKa = 4.45IKK240 pKa = 10.79DD241 pKa = 3.76GYY243 pKa = 10.39IVDD246 pKa = 4.13AMYY249 pKa = 11.23GNYY252 pKa = 9.52KK253 pKa = 9.85EE254 pKa = 5.82AIFDD258 pKa = 3.83PPIVVIFTNLIIDD271 pKa = 4.54GKK273 pKa = 10.38LSEE276 pKa = 4.67SLSPDD281 pKa = 2.45RR282 pKa = 11.84WLKK285 pKa = 9.58LTIDD289 pKa = 3.45NKK291 pKa = 11.11KK292 pKa = 9.9NIVYY296 pKa = 10.69NGIAWNAYY304 pKa = 7.17ITEE307 pKa = 4.39TPLKK311 pKa = 10.31DD312 pKa = 3.28IEE314 pKa = 4.35PEE316 pKa = 3.87EE317 pKa = 4.1FAKK320 pKa = 10.87NLSTKK325 pKa = 10.65YY326 pKa = 10.25LGEE329 pKa = 4.1KK330 pKa = 9.9

Molecular weight: 37.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G8G1|A0A126G8G1_9VIRU Replication associated protein OS=Lake Sarah-associated circular molecule 10 OX=1685725 PE=4 SV=1
MM1 pKa = 6.78TTDD4 pKa = 5.19KK5 pKa = 11.24KK6 pKa = 10.43FTTQNNTKK14 pKa = 8.7YY15 pKa = 9.63WCFTWDD21 pKa = 3.38TNISQKK27 pKa = 10.69KK28 pKa = 8.72LPPVADD34 pKa = 5.41LINFLNNISEE44 pKa = 4.17TAIFQEE50 pKa = 4.19EE51 pKa = 4.91CGTIKK56 pKa = 10.84GKK58 pKa = 8.3VHH60 pKa = 5.26YY61 pKa = 10.15QGTLTLIGPRR71 pKa = 11.84QSKK74 pKa = 10.38ISVLRR79 pKa = 11.84LFEE82 pKa = 4.19RR83 pKa = 11.84RR84 pKa = 11.84FKK86 pKa = 10.96GVSGLTLSRR95 pKa = 11.84SYY97 pKa = 11.5SNQASEE103 pKa = 5.47DD104 pKa = 4.09YY105 pKa = 9.33VTKK108 pKa = 10.76DD109 pKa = 3.02EE110 pKa = 4.41GRR112 pKa = 11.84ISGPYY117 pKa = 9.45FCGKK121 pKa = 9.8KK122 pKa = 9.94EE123 pKa = 4.06KK124 pKa = 10.69FSMEE128 pKa = 3.86YY129 pKa = 9.37STTKK133 pKa = 9.16LTAWQQDD140 pKa = 3.55LFDD143 pKa = 5.22FLTKK147 pKa = 9.16ATKK150 pKa = 9.66IKK152 pKa = 10.14YY153 pKa = 9.05FRR155 pKa = 11.84DD156 pKa = 3.58RR157 pKa = 11.84IVIVVQDD164 pKa = 3.67EE165 pKa = 4.34KK166 pKa = 11.59GNSGKK171 pKa = 10.69SFFLKK176 pKa = 9.89WLAIGQKK183 pKa = 9.76VLNSKK188 pKa = 9.96KK189 pKa = 10.75LPVTSVDD196 pKa = 3.37RR197 pKa = 11.84LMSAVTKK204 pKa = 9.3VTADD208 pKa = 3.27SKK210 pKa = 11.2RR211 pKa = 11.84IDD213 pKa = 3.99LFTINLTRR221 pKa = 11.84SKK223 pKa = 11.42GEE225 pKa = 3.86DD226 pKa = 3.25QSYY229 pKa = 11.23KK230 pKa = 11.04DD231 pKa = 3.53LFAAIEE237 pKa = 4.33EE238 pKa = 4.45IKK240 pKa = 10.79DD241 pKa = 3.76GYY243 pKa = 10.39IVDD246 pKa = 4.13AMYY249 pKa = 11.23GNYY252 pKa = 9.52KK253 pKa = 9.85EE254 pKa = 5.82AIFDD258 pKa = 3.83PPIVVIFTNLIIDD271 pKa = 4.54GKK273 pKa = 10.38LSEE276 pKa = 4.67SLSPDD281 pKa = 2.45RR282 pKa = 11.84WLKK285 pKa = 9.58LTIDD289 pKa = 3.45NKK291 pKa = 11.11KK292 pKa = 9.9NIVYY296 pKa = 10.69NGIAWNAYY304 pKa = 7.17ITEE307 pKa = 4.39TPLKK311 pKa = 10.31DD312 pKa = 3.28IEE314 pKa = 4.35PEE316 pKa = 3.87EE317 pKa = 4.1FAKK320 pKa = 10.87NLSTKK325 pKa = 10.65YY326 pKa = 10.25LGEE329 pKa = 4.1KK330 pKa = 9.9

Molecular weight: 37.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

330

330

330

330.0

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.545 ± 0.0

0.909 ± 0.0

6.364 ± 0.0

5.758 ± 0.0

5.455 ± 0.0

5.455 ± 0.0

0.303 ± 0.0

8.182 ± 0.0

11.212 ± 0.0

8.485 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.212 ± 0.0

5.152 ± 0.0

3.03 ± 0.0

3.333 ± 0.0

3.636 ± 0.0

7.273 ± 0.0

8.485 ± 0.0

5.152 ± 0.0

1.818 ± 0.0

4.242 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski