Sphingomonas rubra

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5U2V0|A0A1I5U2V0_9SPHN Imidazole glycerol phosphate synthase subunit HisF OS=Sphingomonas rubra OX=634430 GN=hisF PE=3 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84LSHH5 pKa = 6.97LLLGGLALAVTTPALAQDD23 pKa = 3.7DD24 pKa = 4.46TAPPKK29 pKa = 10.63AVTVSGSVGLTSDD42 pKa = 3.2YY43 pKa = 10.86RR44 pKa = 11.84FRR46 pKa = 11.84GVSQSDD52 pKa = 3.36EE53 pKa = 3.96NLAVQGGITVSHH65 pKa = 6.76EE66 pKa = 4.07SGVYY70 pKa = 9.83AGVWGSNLAGWGTFGGANMEE90 pKa = 4.45LDD92 pKa = 4.89LIAGVKK98 pKa = 9.99VPVGGGTLDD107 pKa = 4.86VGATWYY113 pKa = 7.91MYY115 pKa = 10.43PGGFDD120 pKa = 2.99NTDD123 pKa = 3.69FIEE126 pKa = 5.02PYY128 pKa = 10.31ARR130 pKa = 11.84LSGTVGPVGLTAGVAYY146 pKa = 10.16APKK149 pKa = 9.91QQALGAWYY157 pKa = 10.55SSGAVAATGVYY168 pKa = 10.31DD169 pKa = 5.8DD170 pKa = 5.67PGDD173 pKa = 4.06KK174 pKa = 10.69NDD176 pKa = 5.24NLYY179 pKa = 11.05LWGDD183 pKa = 3.54VSTAVPDD190 pKa = 3.32TGLTVKK196 pKa = 10.71AHH198 pKa = 6.62LGYY201 pKa = 11.21SNGNKK206 pKa = 10.09GLGPFATSVAPTGEE220 pKa = 4.22YY221 pKa = 10.62VDD223 pKa = 3.72WLAGVDD229 pKa = 3.75YY230 pKa = 8.72TVPGTPLTVGVAYY243 pKa = 10.76VDD245 pKa = 3.56TDD247 pKa = 2.93IDD249 pKa = 3.74RR250 pKa = 11.84AEE252 pKa = 3.94AAYY255 pKa = 9.22LQPSFSRR262 pKa = 11.84GQDD265 pKa = 3.3GVGSIADD272 pKa = 3.51ATVLFTLTAAFF283 pKa = 4.11

Molecular weight: 28.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5QYE4|A0A1I5QYE4_9SPHN Outer membrane receptor proteins mostly Fe transport OS=Sphingomonas rubra OX=634430 GN=SAMN04488241_102383 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3070

0

3070

947806

39

1668

308.7

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.506 ± 0.078

0.7 ± 0.014

6.117 ± 0.038

5.112 ± 0.042

3.316 ± 0.033

9.155 ± 0.039

1.933 ± 0.022

4.418 ± 0.027

2.486 ± 0.037

9.865 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.022

2.223 ± 0.031

5.483 ± 0.034

2.911 ± 0.025

8.004 ± 0.045

4.643 ± 0.029

5.583 ± 0.035

7.777 ± 0.03

1.432 ± 0.019

2.087 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski