Nocardia puris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366DR90|A0A366DR90_9NOCA EmrB/QacA subfamily drug resistance transporter OS=Nocardia puris OX=208602 GN=DFR74_103255 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 4.56GAKK5 pKa = 10.36DD6 pKa = 3.44IPYY9 pKa = 10.01VDD11 pKa = 3.71AAFDD15 pKa = 4.01VNLDD19 pKa = 3.73APDD22 pKa = 3.61IEE24 pKa = 4.82INYY27 pKa = 9.82QLTCRR32 pKa = 11.84FFFEE36 pKa = 4.98NDD38 pKa = 2.75VDD40 pKa = 4.14PPAPAEE46 pKa = 3.87ATEE49 pKa = 3.74FMRR52 pKa = 11.84DD53 pKa = 3.43YY54 pKa = 10.86GVSYY58 pKa = 11.36ALGYY62 pKa = 10.69VNAALASDD70 pKa = 3.96SSAFRR75 pKa = 11.84VRR77 pKa = 11.84NYY79 pKa = 10.28AIPVLALNDD88 pKa = 3.55VKK90 pKa = 11.36SLVTSNEE97 pKa = 3.92SDD99 pKa = 3.06TDD101 pKa = 3.84TPGG104 pKa = 2.92

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366DL82|A0A366DL82_9NOCA Uncharacterized protein OS=Nocardia puris OX=208602 GN=DFR74_10597 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7050

0

7050

2243064

26

13198

318.2

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.786 ± 0.045

0.731 ± 0.009

6.226 ± 0.026

5.644 ± 0.026

2.898 ± 0.018

8.963 ± 0.031

2.258 ± 0.014

3.843 ± 0.02

1.797 ± 0.021

10.169 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.771 ± 0.014

1.859 ± 0.015

6.125 ± 0.03

2.561 ± 0.017

8.069 ± 0.035

5.013 ± 0.02

6.166 ± 0.027

8.616 ± 0.032

1.464 ± 0.012

2.04 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski