Clostridium septicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386PH36|A0A386PH36_CLOSE Carbohydrate ABC transporter substrate-binding protein OS=Clostridium septicum OX=1504 GN=CP523_10000 PE=4 SV=1
MM1 pKa = 7.83EE2 pKa = 4.87FTTIEE7 pKa = 4.07QFRR10 pKa = 11.84EE11 pKa = 3.81QPIEE15 pKa = 3.9VQKK18 pKa = 11.03IFLDD22 pKa = 3.32WWEE25 pKa = 4.24CDD27 pKa = 3.58YY28 pKa = 12.02GDD30 pKa = 4.24LYY32 pKa = 11.34YY33 pKa = 11.21YY34 pKa = 10.94NEE36 pKa = 5.11DD37 pKa = 3.01PHH39 pKa = 7.23EE40 pKa = 4.38YY41 pKa = 10.32KK42 pKa = 10.62DD43 pKa = 5.09VEE45 pKa = 4.9IIDD48 pKa = 4.26NNLEE52 pKa = 3.97CDD54 pKa = 3.6LNGDD58 pKa = 3.67FDD60 pKa = 4.43YY61 pKa = 10.71FKK63 pKa = 10.98SIGPIPLFTEE73 pKa = 4.1GQLRR77 pKa = 11.84KK78 pKa = 9.84FIEE81 pKa = 4.63DD82 pKa = 3.23KK83 pKa = 11.12TNGKK87 pKa = 9.29VEE89 pKa = 4.6SYY91 pKa = 10.3YY92 pKa = 11.21AWDD95 pKa = 4.13YY96 pKa = 8.73YY97 pKa = 10.79TIAIRR102 pKa = 11.84DD103 pKa = 4.03TGCGGDD109 pKa = 3.79DD110 pKa = 3.44PQYY113 pKa = 10.66DD114 pKa = 3.96TEE116 pKa = 4.4EE117 pKa = 4.29TNLLQVYY124 pKa = 8.28WKK126 pKa = 8.8VACIIAEE133 pKa = 4.19EE134 pKa = 4.17KK135 pKa = 10.46VQVSEE140 pKa = 4.3YY141 pKa = 9.04QQ142 pKa = 3.0

Molecular weight: 16.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386PH93|A0A386PH93_CLOSE ROK family protein OS=Clostridium septicum OX=1504 GN=CP523_07245 PE=3 SV=1
MM1 pKa = 7.29ARR3 pKa = 11.84KK4 pKa = 9.71AIIEE8 pKa = 3.95KK9 pKa = 9.52WKK11 pKa = 10.7RR12 pKa = 11.84EE13 pKa = 4.07PKK15 pKa = 10.03YY16 pKa = 9.5STRR19 pKa = 11.84AYY21 pKa = 8.1TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.74SVLKK35 pKa = 10.88KK36 pKa = 10.24FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.88KK50 pKa = 10.17GQIPGCKK57 pKa = 8.74KK58 pKa = 10.74ASWW61 pKa = 3.34

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2908

0

2908

919036

27

2835

316.0

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.313 ± 0.049

1.073 ± 0.018

5.527 ± 0.035

7.711 ± 0.058

4.38 ± 0.039

6.427 ± 0.037

1.219 ± 0.013

10.272 ± 0.056

9.325 ± 0.047

9.095 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.023

6.862 ± 0.048

2.648 ± 0.022

2.068 ± 0.019

3.279 ± 0.029

6.279 ± 0.038

4.859 ± 0.029

6.444 ± 0.04

0.68 ± 0.014

4.106 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski