Microbacterium phage DizzyRudy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T675|A0A4D6T675_9CAUD Uncharacterized protein OS=Microbacterium phage DizzyRudy OX=2572531 GN=13 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84YY3 pKa = 10.16ALVKK7 pKa = 10.88DD8 pKa = 3.78NVVCSVILWDD18 pKa = 4.43GEE20 pKa = 4.46TPYY23 pKa = 10.69EE24 pKa = 4.15PEE26 pKa = 4.47EE27 pKa = 4.51GCVMYY32 pKa = 9.46EE33 pKa = 4.11VPDD36 pKa = 4.53EE37 pKa = 4.52ISPGWTIADD46 pKa = 3.98NQWAPPIPVEE56 pKa = 3.98IPVPSEE62 pKa = 3.78TEE64 pKa = 4.05AIEE67 pKa = 4.04TAKK70 pKa = 10.38IQALLEE76 pKa = 4.04LTALGMSEE84 pKa = 3.61ATARR88 pKa = 11.84TIVGLPPIDD97 pKa = 3.68SQEE100 pKa = 3.72

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T6B9|A0A4D6T6B9_9CAUD Holliday junction resolvase OS=Microbacterium phage DizzyRudy OX=2572531 GN=83 PE=4 SV=1
MM1 pKa = 7.18RR2 pKa = 11.84CKK4 pKa = 10.48RR5 pKa = 11.84KK6 pKa = 8.81MKK8 pKa = 10.34KK9 pKa = 9.43GWYY12 pKa = 9.07CVRR15 pKa = 11.84TQHH18 pKa = 7.64DD19 pKa = 4.1SGPCALRR26 pKa = 11.84PRR28 pKa = 11.84WWNIAGKK35 pKa = 9.65IKK37 pKa = 10.03HH38 pKa = 6.29RR39 pKa = 11.84NYY41 pKa = 10.87LYY43 pKa = 11.06

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

17291

15

1402

201.1

22.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.305 ± 0.617

0.474 ± 0.081

6.396 ± 0.265

6.969 ± 0.39

3.765 ± 0.199

7.657 ± 0.502

1.885 ± 0.185

5.76 ± 0.276

5.899 ± 0.413

7.831 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.276

4.343 ± 0.12

4.135 ± 0.293

3.256 ± 0.148

5.442 ± 0.401

5.957 ± 0.248

6.119 ± 0.272

7.061 ± 0.219

1.544 ± 0.156

3.499 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski