Amino acid dipepetide frequency for Mycobacterium phage Donkeykong

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.086AlaAla: 15.086 ± 1.85
0.733AlaCys: 0.733 ± 0.19
7.543AlaAsp: 7.543 ± 0.622
7.543AlaGlu: 7.543 ± 0.714
2.567AlaPhe: 2.567 ± 0.41
9.9AlaGly: 9.9 ± 1.042
2.252AlaHis: 2.252 ± 0.425
4.033AlaIle: 4.033 ± 0.512
3.876AlaLys: 3.876 ± 0.386
8.486AlaLeu: 8.486 ± 0.879
2.724AlaMet: 2.724 ± 0.399
2.41AlaAsn: 2.41 ± 0.303
5.552AlaPro: 5.552 ± 0.617
3.195AlaGln: 3.195 ± 0.449
8.59AlaArg: 8.59 ± 0.989
4.976AlaSer: 4.976 ± 0.616
6.338AlaThr: 6.338 ± 0.598
6.652AlaVal: 6.652 ± 0.653
3.3AlaTrp: 3.3 ± 0.536
2.514AlaTyr: 2.514 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
1.205CysAla: 1.205 ± 0.322
0.105CysCys: 0.105 ± 0.072
1.414CysAsp: 1.414 ± 0.336
0.838CysGlu: 0.838 ± 0.244
0.21CysPhe: 0.21 ± 0.104
1.624CysGly: 1.624 ± 0.346
0.314CysHis: 0.314 ± 0.121
0.105CysIle: 0.105 ± 0.075
0.733CysLys: 0.733 ± 0.187
0.681CysLeu: 0.681 ± 0.206
0.314CysMet: 0.314 ± 0.12
0.524CysAsn: 0.524 ± 0.166
0.89CysPro: 0.89 ± 0.249
0.314CysGln: 0.314 ± 0.136
0.786CysArg: 0.786 ± 0.216
0.786CysSer: 0.786 ± 0.232
0.786CysThr: 0.786 ± 0.215
0.786CysVal: 0.786 ± 0.195
0.262CysTrp: 0.262 ± 0.096
0.262CysTyr: 0.262 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
7.071AspAla: 7.071 ± 0.644
1.205AspCys: 1.205 ± 0.288
4.767AspAsp: 4.767 ± 0.515
3.667AspGlu: 3.667 ± 0.402
1.99AspPhe: 1.99 ± 0.271
6.914AspGly: 6.914 ± 0.64
1.519AspHis: 1.519 ± 0.305
2.776AspIle: 2.776 ± 0.35
1.729AspLys: 1.729 ± 0.273
6.024AspLeu: 6.024 ± 0.486
1.31AspMet: 1.31 ± 0.284
1.362AspAsn: 1.362 ± 0.283
4.924AspPro: 4.924 ± 0.608
2.933AspGln: 2.933 ± 0.393
5.133AspArg: 5.133 ± 0.61
3.352AspSer: 3.352 ± 0.498
3.824AspThr: 3.824 ± 0.409
3.981AspVal: 3.981 ± 0.494
1.519AspTrp: 1.519 ± 0.305
1.676AspTyr: 1.676 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
6.6GluAla: 6.6 ± 0.606
0.786GluCys: 0.786 ± 0.207
3.981GluAsp: 3.981 ± 0.413
2.881GluGlu: 2.881 ± 0.463
2.305GluPhe: 2.305 ± 0.351
3.248GluGly: 3.248 ± 0.422
1.624GluHis: 1.624 ± 0.323
2.41GluIle: 2.41 ± 0.401
1.938GluLys: 1.938 ± 0.325
5.762GluLeu: 5.762 ± 0.726
1.571GluMet: 1.571 ± 0.332
1.729GluAsn: 1.729 ± 0.253
3.352GluPro: 3.352 ± 0.462
3.195GluGln: 3.195 ± 0.392
5.029GluArg: 5.029 ± 0.677
3.195GluSer: 3.195 ± 0.4
3.667GluThr: 3.667 ± 0.53
3.929GluVal: 3.929 ± 0.501
1.624GluTrp: 1.624 ± 0.28
1.886GluTyr: 1.886 ± 0.325
0.0GluXaa: 0.0 ± 0.0
Phe
2.829PheAla: 2.829 ± 0.37
0.367PheCys: 0.367 ± 0.141
2.41PheAsp: 2.41 ± 0.405
1.624PheGlu: 1.624 ± 0.326
0.786PhePhe: 0.786 ± 0.291
2.619PheGly: 2.619 ± 0.6
0.576PheHis: 0.576 ± 0.176
1.467PheIle: 1.467 ± 0.329
0.838PheLys: 0.838 ± 0.203
1.833PheLeu: 1.833 ± 0.246
0.786PheMet: 0.786 ± 0.236
1.048PheAsn: 1.048 ± 0.341
1.519PhePro: 1.519 ± 0.306
0.838PheGln: 0.838 ± 0.277
1.676PheArg: 1.676 ± 0.29
1.467PheSer: 1.467 ± 0.256
2.305PheThr: 2.305 ± 0.354
2.252PheVal: 2.252 ± 0.347
0.524PheTrp: 0.524 ± 0.132
0.995PheTyr: 0.995 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
8.276GlyAla: 8.276 ± 1.326
1.205GlyCys: 1.205 ± 0.294
6.338GlyAsp: 6.338 ± 0.565
4.033GlyGlu: 4.033 ± 0.561
2.567GlyPhe: 2.567 ± 0.42
10.843GlyGly: 10.843 ± 2.193
1.571GlyHis: 1.571 ± 0.253
4.243GlyIle: 4.243 ± 0.629
2.724GlyLys: 2.724 ± 0.399
5.5GlyLeu: 5.5 ± 0.568
2.776GlyMet: 2.776 ± 0.438
2.933GlyAsn: 2.933 ± 0.348
4.243GlyPro: 4.243 ± 0.751
2.724GlyGln: 2.724 ± 0.541
5.029GlyArg: 5.029 ± 0.684
6.024GlySer: 6.024 ± 0.802
6.338GlyThr: 6.338 ± 0.708
6.338GlyVal: 6.338 ± 0.6
2.2GlyTrp: 2.2 ± 0.341
2.148GlyTyr: 2.148 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
1.781HisAla: 1.781 ± 0.338
0.314HisCys: 0.314 ± 0.145
0.995HisAsp: 0.995 ± 0.187
1.414HisGlu: 1.414 ± 0.32
0.419HisPhe: 0.419 ± 0.151
1.676HisGly: 1.676 ± 0.266
1.048HisHis: 1.048 ± 0.303
1.519HisIle: 1.519 ± 0.325
0.838HisLys: 0.838 ± 0.217
1.571HisLeu: 1.571 ± 0.301
0.471HisMet: 0.471 ± 0.118
1.048HisAsn: 1.048 ± 0.276
1.571HisPro: 1.571 ± 0.27
0.786HisGln: 0.786 ± 0.199
2.41HisArg: 2.41 ± 0.374
0.786HisSer: 0.786 ± 0.186
1.676HisThr: 1.676 ± 0.357
0.995HisVal: 0.995 ± 0.317
0.576HisTrp: 0.576 ± 0.155
0.576HisTyr: 0.576 ± 0.138
0.0HisXaa: 0.0 ± 0.0
Ile
5.029IleAla: 5.029 ± 0.494
0.576IleCys: 0.576 ± 0.189
3.876IleAsp: 3.876 ± 0.438
4.086IleGlu: 4.086 ± 0.329
0.838IlePhe: 0.838 ± 0.231
4.243IleGly: 4.243 ± 0.504
1.257IleHis: 1.257 ± 0.234
1.1IleIle: 1.1 ± 0.255
1.152IleLys: 1.152 ± 0.243
2.043IleLeu: 2.043 ± 0.439
0.367IleMet: 0.367 ± 0.124
1.781IleAsn: 1.781 ± 0.271
2.986IlePro: 2.986 ± 0.299
1.257IleGln: 1.257 ± 0.238
2.986IleArg: 2.986 ± 0.486
2.043IleSer: 2.043 ± 0.479
3.771IleThr: 3.771 ± 0.477
2.986IleVal: 2.986 ± 0.444
0.995IleTrp: 0.995 ± 0.276
0.629IleTyr: 0.629 ± 0.176
0.0IleXaa: 0.0 ± 0.0
Lys
4.452LysAla: 4.452 ± 0.511
0.471LysCys: 0.471 ± 0.151
1.781LysAsp: 1.781 ± 0.291
1.152LysGlu: 1.152 ± 0.236
1.152LysPhe: 1.152 ± 0.209
2.619LysGly: 2.619 ± 0.374
1.048LysHis: 1.048 ± 0.26
0.995LysIle: 0.995 ± 0.223
1.1LysLys: 1.1 ± 0.282
2.829LysLeu: 2.829 ± 0.458
0.681LysMet: 0.681 ± 0.191
0.838LysAsn: 0.838 ± 0.208
1.938LysPro: 1.938 ± 0.32
1.519LysGln: 1.519 ± 0.263
2.567LysArg: 2.567 ± 0.36
1.676LysSer: 1.676 ± 0.229
2.148LysThr: 2.148 ± 0.389
2.357LysVal: 2.357 ± 0.343
0.681LysTrp: 0.681 ± 0.169
0.943LysTyr: 0.943 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
8.171LeuAla: 8.171 ± 0.792
0.786LeuCys: 0.786 ± 0.213
5.081LeuAsp: 5.081 ± 0.604
3.667LeuGlu: 3.667 ± 0.483
2.252LeuPhe: 2.252 ± 0.276
5.709LeuGly: 5.709 ± 0.617
0.995LeuHis: 0.995 ± 0.224
3.457LeuIle: 3.457 ± 0.492
1.833LeuLys: 1.833 ± 0.304
4.557LeuLeu: 4.557 ± 0.454
1.729LeuMet: 1.729 ± 0.369
2.357LeuAsn: 2.357 ± 0.336
5.448LeuPro: 5.448 ± 0.645
2.357LeuGln: 2.357 ± 0.369
6.024LeuArg: 6.024 ± 0.63
4.452LeuSer: 4.452 ± 0.531
6.286LeuThr: 6.286 ± 0.553
5.029LeuVal: 5.029 ± 0.483
1.414LeuTrp: 1.414 ± 0.271
2.305LeuTyr: 2.305 ± 0.385
0.0LeuXaa: 0.0 ± 0.0
Met
2.41MetAla: 2.41 ± 0.441
0.262MetCys: 0.262 ± 0.116
1.467MetAsp: 1.467 ± 0.238
1.048MetGlu: 1.048 ± 0.213
0.943MetPhe: 0.943 ± 0.237
1.729MetGly: 1.729 ± 0.3
0.105MetHis: 0.105 ± 0.07
0.89MetIle: 0.89 ± 0.23
0.786MetLys: 0.786 ± 0.197
1.729MetLeu: 1.729 ± 0.271
0.733MetMet: 0.733 ± 0.192
0.786MetAsn: 0.786 ± 0.178
1.414MetPro: 1.414 ± 0.241
0.367MetGln: 0.367 ± 0.115
1.624MetArg: 1.624 ± 0.283
3.195MetSer: 3.195 ± 0.407
1.938MetThr: 1.938 ± 0.231
1.257MetVal: 1.257 ± 0.288
0.157MetTrp: 0.157 ± 0.091
0.471MetTyr: 0.471 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.719AsnAla: 3.719 ± 0.373
0.367AsnCys: 0.367 ± 0.162
1.886AsnAsp: 1.886 ± 0.243
1.571AsnGlu: 1.571 ± 0.295
0.786AsnPhe: 0.786 ± 0.243
3.771AsnGly: 3.771 ± 0.503
1.152AsnHis: 1.152 ± 0.266
1.467AsnIle: 1.467 ± 0.481
0.786AsnLys: 0.786 ± 0.184
2.305AsnLeu: 2.305 ± 0.38
0.629AsnMet: 0.629 ± 0.151
1.624AsnAsn: 1.624 ± 0.306
2.2AsnPro: 2.2 ± 0.309
1.257AsnGln: 1.257 ± 0.322
1.781AsnArg: 1.781 ± 0.333
1.467AsnSer: 1.467 ± 0.247
2.095AsnThr: 2.095 ± 0.355
1.571AsnVal: 1.571 ± 0.319
0.733AsnTrp: 0.733 ± 0.224
0.681AsnTyr: 0.681 ± 0.154
0.0AsnXaa: 0.0 ± 0.0
Pro
5.395ProAla: 5.395 ± 0.586
0.838ProCys: 0.838 ± 0.209
4.662ProAsp: 4.662 ± 0.615
4.976ProGlu: 4.976 ± 0.578
1.833ProPhe: 1.833 ± 0.272
6.129ProGly: 6.129 ± 0.664
1.624ProHis: 1.624 ± 0.258
2.043ProIle: 2.043 ± 0.316
2.252ProLys: 2.252 ± 0.342
4.557ProLeu: 4.557 ± 0.507
1.571ProMet: 1.571 ± 0.347
2.305ProAsn: 2.305 ± 0.284
3.3ProPro: 3.3 ± 0.539
1.938ProGln: 1.938 ± 0.304
3.457ProArg: 3.457 ± 0.494
2.881ProSer: 2.881 ± 0.424
3.09ProThr: 3.09 ± 0.434
4.295ProVal: 4.295 ± 0.483
1.152ProTrp: 1.152 ± 0.225
1.362ProTyr: 1.362 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
5.133GlnAla: 5.133 ± 0.572
0.157GlnCys: 0.157 ± 0.089
1.31GlnAsp: 1.31 ± 0.259
1.676GlnGlu: 1.676 ± 0.315
1.257GlnPhe: 1.257 ± 0.22
2.357GlnGly: 2.357 ± 0.507
0.681GlnHis: 0.681 ± 0.186
1.833GlnIle: 1.833 ± 0.305
1.414GlnLys: 1.414 ± 0.256
3.038GlnLeu: 3.038 ± 0.377
0.786GlnMet: 0.786 ± 0.163
0.681GlnAsn: 0.681 ± 0.215
2.567GlnPro: 2.567 ± 0.38
1.152GlnGln: 1.152 ± 0.261
1.886GlnArg: 1.886 ± 0.319
1.833GlnSer: 1.833 ± 0.369
1.833GlnThr: 1.833 ± 0.381
2.619GlnVal: 2.619 ± 0.332
0.524GlnTrp: 0.524 ± 0.141
0.786GlnTyr: 0.786 ± 0.253
0.0GlnXaa: 0.0 ± 0.0
Arg
6.757ArgAla: 6.757 ± 0.709
1.571ArgCys: 1.571 ± 0.373
4.138ArgAsp: 4.138 ± 0.492
5.133ArgGlu: 5.133 ± 0.654
2.252ArgPhe: 2.252 ± 0.377
4.452ArgGly: 4.452 ± 0.558
1.781ArgHis: 1.781 ± 0.333
3.614ArgIle: 3.614 ± 0.496
2.986ArgLys: 2.986 ± 0.491
5.605ArgLeu: 5.605 ± 0.735
2.095ArgMet: 2.095 ± 0.321
2.514ArgAsn: 2.514 ± 0.367
3.09ArgPro: 3.09 ± 0.37
2.095ArgGln: 2.095 ± 0.398
6.39ArgArg: 6.39 ± 0.834
3.981ArgSer: 3.981 ± 0.576
3.143ArgThr: 3.143 ± 0.457
5.762ArgVal: 5.762 ± 0.653
1.938ArgTrp: 1.938 ± 0.331
1.99ArgTyr: 1.99 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
6.548SerAla: 6.548 ± 1.177
0.471SerCys: 0.471 ± 0.221
3.614SerAsp: 3.614 ± 0.371
3.248SerGlu: 3.248 ± 0.457
1.676SerPhe: 1.676 ± 0.42
6.286SerGly: 6.286 ± 0.892
1.31SerHis: 1.31 ± 0.238
3.038SerIle: 3.038 ± 0.443
2.357SerLys: 2.357 ± 0.327
3.719SerLeu: 3.719 ± 0.456
1.205SerMet: 1.205 ± 0.278
2.514SerAsn: 2.514 ± 0.417
2.881SerPro: 2.881 ± 0.335
1.519SerGln: 1.519 ± 0.241
2.881SerArg: 2.881 ± 0.356
3.929SerSer: 3.929 ± 0.793
3.248SerThr: 3.248 ± 0.462
4.243SerVal: 4.243 ± 0.483
1.31SerTrp: 1.31 ± 0.273
1.1SerTyr: 1.1 ± 0.198
0.0SerXaa: 0.0 ± 0.0
Thr
6.6ThrAla: 6.6 ± 0.58
0.786ThrCys: 0.786 ± 0.202
4.19ThrAsp: 4.19 ± 0.525
4.086ThrGlu: 4.086 ± 0.43
1.729ThrPhe: 1.729 ± 0.315
5.814ThrGly: 5.814 ± 0.753
1.676ThrHis: 1.676 ± 0.313
3.667ThrIle: 3.667 ± 0.438
1.99ThrLys: 1.99 ± 0.304
4.976ThrLeu: 4.976 ± 0.561
1.1ThrMet: 1.1 ± 0.226
1.833ThrAsn: 1.833 ± 0.331
4.61ThrPro: 4.61 ± 0.497
1.886ThrGln: 1.886 ± 0.274
4.033ThrArg: 4.033 ± 0.429
3.719ThrSer: 3.719 ± 0.464
4.61ThrThr: 4.61 ± 0.624
5.552ThrVal: 5.552 ± 0.637
1.048ThrTrp: 1.048 ± 0.267
1.624ThrTyr: 1.624 ± 0.263
0.0ThrXaa: 0.0 ± 0.0
Val
6.705ValAla: 6.705 ± 0.59
1.31ValCys: 1.31 ± 0.32
5.395ValAsp: 5.395 ± 0.512
5.081ValGlu: 5.081 ± 0.571
1.886ValPhe: 1.886 ± 0.354
5.238ValGly: 5.238 ± 0.615
0.995ValHis: 0.995 ± 0.195
2.933ValIle: 2.933 ± 0.415
2.043ValLys: 2.043 ± 0.367
4.924ValLeu: 4.924 ± 0.567
1.31ValMet: 1.31 ± 0.204
2.252ValAsn: 2.252 ± 0.343
4.4ValPro: 4.4 ± 0.39
2.462ValGln: 2.462 ± 0.365
4.714ValArg: 4.714 ± 0.649
4.767ValSer: 4.767 ± 0.545
5.081ValThr: 5.081 ± 0.536
5.867ValVal: 5.867 ± 0.561
1.676ValTrp: 1.676 ± 0.315
1.467ValTyr: 1.467 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
1.99TrpAla: 1.99 ± 0.272
0.262TrpCys: 0.262 ± 0.109
1.519TrpAsp: 1.519 ± 0.264
1.048TrpGlu: 1.048 ± 0.294
0.838TrpPhe: 0.838 ± 0.171
1.152TrpGly: 1.152 ± 0.223
0.419TrpHis: 0.419 ± 0.136
1.205TrpIle: 1.205 ± 0.227
0.943TrpLys: 0.943 ± 0.204
1.99TrpLeu: 1.99 ± 0.379
0.838TrpMet: 0.838 ± 0.219
0.681TrpAsn: 0.681 ± 0.219
1.1TrpPro: 1.1 ± 0.271
0.838TrpGln: 0.838 ± 0.272
2.148TrpArg: 2.148 ± 0.392
1.519TrpSer: 1.519 ± 0.341
1.571TrpThr: 1.571 ± 0.238
1.676TrpVal: 1.676 ± 0.383
0.89TrpTrp: 0.89 ± 0.201
0.419TrpTyr: 0.419 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.514TyrAla: 2.514 ± 0.355
0.367TyrCys: 0.367 ± 0.155
1.31TyrAsp: 1.31 ± 0.326
1.99TyrGlu: 1.99 ± 0.338
0.471TyrPhe: 0.471 ± 0.164
1.729TyrGly: 1.729 ± 0.348
0.524TyrHis: 0.524 ± 0.16
1.205TyrIle: 1.205 ± 0.234
0.629TyrLys: 0.629 ± 0.176
1.781TyrLeu: 1.781 ± 0.298
0.262TyrMet: 0.262 ± 0.102
0.629TyrAsn: 0.629 ± 0.164
1.676TyrPro: 1.676 ± 0.23
0.89TyrGln: 0.89 ± 0.231
2.148TyrArg: 2.148 ± 0.37
1.048TyrSer: 1.048 ± 0.265
1.781TyrThr: 1.781 ± 0.331
2.357TyrVal: 2.357 ± 0.361
0.524TyrTrp: 0.524 ± 0.184
0.629TyrTyr: 0.629 ± 0.152
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 110 proteins (19092 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski