Fusarium culmorum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales;

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12360 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4GEL4|A0A2T4GEL4_FUSCU Uncharacterized protein OS=Fusarium culmorum OX=5516 GN=FCULG_00010253 PE=4 SV=1
MM1 pKa = 7.82AYY3 pKa = 10.66SLTTSFIGEE12 pKa = 4.16SLLSGFEE19 pKa = 4.16WFNGTDD25 pKa = 3.4LSKK28 pKa = 11.38GFVQYY33 pKa = 11.13QDD35 pKa = 3.2FTGAFEE41 pKa = 4.39NGLYY45 pKa = 10.65SVDD48 pKa = 3.8PFSEE52 pKa = 4.77TVQLRR57 pKa = 11.84PDD59 pKa = 3.29SRR61 pKa = 11.84HH62 pKa = 6.33KK63 pKa = 11.06YY64 pKa = 10.08GLDD67 pKa = 3.0EE68 pKa = 4.46GRR70 pKa = 11.84PSIRR74 pKa = 11.84LEE76 pKa = 4.12SKK78 pKa = 10.14EE79 pKa = 4.04SYY81 pKa = 10.47QYY83 pKa = 11.98GLFIADD89 pKa = 4.16FQHH92 pKa = 6.63MPISQCGTWPAFWAYY107 pKa = 9.91GSNWPMGGEE116 pKa = 3.82IDD118 pKa = 3.63ILEE121 pKa = 4.64GANLAYY127 pKa = 9.51TNIMSAHH134 pKa = 5.16TAEE137 pKa = 4.57GCMLDD142 pKa = 3.98PADD145 pKa = 4.36SNLFSGIRR153 pKa = 11.84RR154 pKa = 11.84NLDD157 pKa = 3.17CGVGTNNVGCGFNPPKK173 pKa = 10.45SDD175 pKa = 2.97TSSYY179 pKa = 11.36GDD181 pKa = 3.35GFNAVGGGVYY191 pKa = 10.63AMEE194 pKa = 4.64WDD196 pKa = 3.6SEE198 pKa = 4.75YY199 pKa = 10.73ISIWHH204 pKa = 6.56FPRR207 pKa = 11.84GAIPADD213 pKa = 3.51IEE215 pKa = 4.53AKK217 pKa = 10.53QPDD220 pKa = 4.02PRR222 pKa = 11.84NWGLPQSLFGGAKK235 pKa = 9.89CDD237 pKa = 3.27VDD239 pKa = 5.41KK240 pKa = 11.48YY241 pKa = 10.76FSDD244 pKa = 3.35MKK246 pKa = 10.93LVLNINFCGDD256 pKa = 3.43YY257 pKa = 11.63GEE259 pKa = 4.7GTWANSKK266 pKa = 9.28VCRR269 pKa = 11.84ALAPTCRR276 pKa = 11.84EE277 pKa = 3.86YY278 pKa = 11.3VANNPTAFKK287 pKa = 10.12EE288 pKa = 4.27AYY290 pKa = 9.86FDD292 pKa = 3.29VSYY295 pKa = 11.1IDD297 pKa = 3.24VYY299 pKa = 11.02TRR301 pKa = 11.84LGGNIPPVLPSSASEE316 pKa = 4.05ASTQASDD323 pKa = 3.69VIIPGEE329 pKa = 4.22SATNTPMSGRR339 pKa = 11.84PVFPSTNFPGNSTIATRR356 pKa = 11.84TDD358 pKa = 3.09RR359 pKa = 11.84KK360 pKa = 10.75GKK362 pKa = 8.6ATQSEE367 pKa = 5.02EE368 pKa = 4.1PTTTTTLTMTGISSVLVTIPGSGTDD393 pKa = 3.71TPTVTPLPVATGGKK407 pKa = 9.0SANPSKK413 pKa = 10.74IGDD416 pKa = 3.7YY417 pKa = 10.88SYY419 pKa = 11.3LGCFGSQNGFQTFDD433 pKa = 3.88LADD436 pKa = 4.78DD437 pKa = 4.29SDD439 pKa = 4.34DD440 pKa = 3.69MTIEE444 pKa = 4.12RR445 pKa = 11.84CVDD448 pKa = 2.91ACNGLTYY455 pKa = 10.29IGLFEE460 pKa = 4.26GTCYY464 pKa = 10.14CASVLDD470 pKa = 4.31GDD472 pKa = 4.1TLAIRR477 pKa = 11.84NEE479 pKa = 4.32TSCNRR484 pKa = 11.84PCPGDD489 pKa = 3.77DD490 pKa = 3.42AQFCGGMVTQNTKK503 pKa = 8.16TRR505 pKa = 11.84FRR507 pKa = 11.84RR508 pKa = 11.84TTPLRR513 pKa = 11.84RR514 pKa = 11.84DD515 pKa = 3.31APSNVLLTVYY525 pKa = 11.16ADD527 pKa = 3.32ISDD530 pKa = 4.02VEE532 pKa = 4.38LPEE535 pKa = 4.35VPPAMGPGTNDD546 pKa = 2.69TSTDD550 pKa = 3.67DD551 pKa = 4.41GDD553 pKa = 4.18SQGDD557 pKa = 3.88SATDD561 pKa = 3.42EE562 pKa = 4.63SEE564 pKa = 4.58SPTTSDD570 pKa = 4.69DD571 pKa = 3.61NASQGASDD579 pKa = 3.51AAEE582 pKa = 4.54PEE584 pKa = 4.01NSGDD588 pKa = 3.69SDD590 pKa = 3.8VTTSQEE596 pKa = 3.81STNADD601 pKa = 2.97GSEE604 pKa = 4.48DD605 pKa = 3.37LTASNDD611 pKa = 3.08NGTQGGSTTDD621 pKa = 4.01PDD623 pKa = 4.82DD624 pKa = 3.8STASDD629 pKa = 3.67GNASQRR635 pKa = 11.84GSEE638 pKa = 4.19SGDD641 pKa = 3.52SKK643 pKa = 10.94ILPVSNDD650 pKa = 3.53NIDD653 pKa = 3.4ASLAGSASSPGSSAQGSSDD672 pKa = 3.69EE673 pKa = 4.51QDD675 pKa = 3.4SSDD678 pKa = 4.94DD679 pKa = 3.85QDD681 pKa = 5.9DD682 pKa = 4.13PDD684 pKa = 4.87SDD686 pKa = 4.22GNSILPSAAGVVLSDD701 pKa = 4.91DD702 pKa = 3.47QDD704 pKa = 3.95TPPVAVIGTASDD716 pKa = 3.86GLVLGATQSAADD728 pKa = 3.84SNQEE732 pKa = 3.8LGIITMSYY740 pKa = 9.57EE741 pKa = 3.67KK742 pKa = 10.21CDD744 pKa = 3.57YY745 pKa = 11.21CEE747 pKa = 3.88TPEE750 pKa = 4.83LIKK753 pKa = 10.74PPMEE757 pKa = 4.28TKK759 pKa = 10.58VVDD762 pKa = 4.44CDD764 pKa = 3.59GCGPNGEE771 pKa = 4.36DD772 pKa = 3.5TVTLTVPISVTVTVPGASTVQAQQTEE798 pKa = 4.27KK799 pKa = 10.76AAAEE803 pKa = 4.12AVTSYY808 pKa = 11.18GDD810 pKa = 3.82SPEE813 pKa = 3.95QPPRR817 pKa = 11.84NSTILPVVPNGMGSGAEE834 pKa = 3.91AGEE837 pKa = 4.41SPEE840 pKa = 3.92VTTVVITYY848 pKa = 7.5LTTQLVTYY856 pKa = 7.47GTSTDD861 pKa = 3.39SVSTHH866 pKa = 6.3LAYY869 pKa = 9.67PIPGQPNTPVTIATPTPGAPSEE891 pKa = 4.1PVAVSAASSRR901 pKa = 11.84RR902 pKa = 11.84DD903 pKa = 3.36DD904 pKa = 3.56VFVYY908 pKa = 8.89FAIVAMAILALTLL921 pKa = 3.7

Molecular weight: 96.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4GI23|A0A2T4GI23_FUSCU ATPase GET3 OS=Fusarium culmorum OX=5516 GN=GET3 PE=3 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APARR26 pKa = 11.84SNRR29 pKa = 11.84HH30 pKa = 4.32TVTTTTTTTTKK41 pKa = 9.91PRR43 pKa = 11.84RR44 pKa = 11.84GMFGGGSTARR54 pKa = 11.84RR55 pKa = 11.84THH57 pKa = 5.96GAAPVHH63 pKa = 5.35HH64 pKa = 6.52HH65 pKa = 5.47QRR67 pKa = 11.84RR68 pKa = 11.84PSMKK72 pKa = 10.02DD73 pKa = 2.95KK74 pKa = 11.41VSGALLKK81 pKa = 11.04LKK83 pKa = 10.68GSLTRR88 pKa = 11.84RR89 pKa = 11.84PGVKK93 pKa = 9.89AAGTRR98 pKa = 11.84RR99 pKa = 11.84MHH101 pKa = 5.56GTNGRR106 pKa = 11.84GARR109 pKa = 11.84HH110 pKa = 5.8HH111 pKa = 7.22RR112 pKa = 11.84YY113 pKa = 9.44

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12360

0

12360

5813542

24

7826

470.4

52.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.269 ± 0.021

1.3 ± 0.01

5.936 ± 0.017

6.207 ± 0.026

3.769 ± 0.014

6.736 ± 0.02

2.362 ± 0.01

5.102 ± 0.016

5.124 ± 0.02

8.679 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.009

3.862 ± 0.01

5.856 ± 0.023

4.015 ± 0.017

5.793 ± 0.02

8.129 ± 0.024

6.124 ± 0.029

6.15 ± 0.018

1.514 ± 0.009

2.796 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski