Amino acid dipepetide frequency for Bacillus phage Shbh1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.766AlaAla: 1.766 ± 0.285
0.333AlaCys: 0.333 ± 0.081
2.738AlaAsp: 2.738 ± 0.289
3.455AlaGlu: 3.455 ± 0.302
1.945AlaPhe: 1.945 ± 0.224
2.559AlaGly: 2.559 ± 0.341
0.947AlaHis: 0.947 ± 0.151
3.557AlaIle: 3.557 ± 0.382
3.25AlaLys: 3.25 ± 0.301
4.274AlaLeu: 4.274 ± 0.47
0.896AlaMet: 0.896 ± 0.156
2.533AlaAsn: 2.533 ± 0.273
1.356AlaPro: 1.356 ± 0.205
1.612AlaGln: 1.612 ± 0.219
2.533AlaArg: 2.533 ± 0.291
3.531AlaSer: 3.531 ± 0.343
3.071AlaThr: 3.071 ± 0.332
2.789AlaVal: 2.789 ± 0.315
0.64AlaTrp: 0.64 ± 0.12
2.098AlaTyr: 2.098 ± 0.218
0.0AlaXaa: 0.0 ± 0.0
Cys
0.23CysAla: 0.23 ± 0.079
0.179CysCys: 0.179 ± 0.076
0.461CysAsp: 0.461 ± 0.128
0.563CysGlu: 0.563 ± 0.148
0.409CysPhe: 0.409 ± 0.118
0.205CysGly: 0.205 ± 0.079
0.281CysHis: 0.281 ± 0.092
0.358CysIle: 0.358 ± 0.108
0.589CysLys: 0.589 ± 0.131
0.64CysLeu: 0.64 ± 0.147
0.179CysMet: 0.179 ± 0.063
0.281CysAsn: 0.281 ± 0.075
0.307CysPro: 0.307 ± 0.085
0.23CysGln: 0.23 ± 0.079
0.307CysArg: 0.307 ± 0.101
0.614CysSer: 0.614 ± 0.1
0.179CysThr: 0.179 ± 0.063
0.461CysVal: 0.461 ± 0.105
0.154CysTrp: 0.154 ± 0.058
0.358CysTyr: 0.358 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
2.15AspAla: 2.15 ± 0.272
0.409AspCys: 0.409 ± 0.101
3.25AspAsp: 3.25 ± 0.355
4.99AspGlu: 4.99 ± 0.425
3.429AspPhe: 3.429 ± 0.284
4.222AspGly: 4.222 ± 0.463
1.433AspHis: 1.433 ± 0.191
5.169AspIle: 5.169 ± 0.374
5.195AspLys: 5.195 ± 0.467
6.474AspLeu: 6.474 ± 0.44
1.74AspMet: 1.74 ± 0.204
3.327AspAsn: 3.327 ± 0.266
2.457AspPro: 2.457 ± 0.26
1.945AspGln: 1.945 ± 0.228
2.968AspArg: 2.968 ± 0.321
4.555AspSer: 4.555 ± 0.397
3.608AspThr: 3.608 ± 0.265
4.504AspVal: 4.504 ± 0.274
0.742AspTrp: 0.742 ± 0.118
4.094AspTyr: 4.094 ± 0.32
0.0AspXaa: 0.0 ± 0.0
Glu
4.171GluAla: 4.171 ± 0.373
0.512GluCys: 0.512 ± 0.119
6.653GluAsp: 6.653 ± 0.533
9.084GluGlu: 9.084 ± 0.771
3.045GluPhe: 3.045 ± 0.244
5.451GluGly: 5.451 ± 0.404
1.459GluHis: 1.459 ± 0.207
5.348GluIle: 5.348 ± 0.375
5.63GluLys: 5.63 ± 0.535
7.728GluLeu: 7.728 ± 0.487
1.842GluMet: 1.842 ± 0.259
4.427GluAsn: 4.427 ± 0.375
1.663GluPro: 1.663 ± 0.216
3.403GluGln: 3.403 ± 0.322
3.506GluArg: 3.506 ± 0.372
4.094GluSer: 4.094 ± 0.321
3.429GluThr: 3.429 ± 0.333
6.781GluVal: 6.781 ± 0.468
0.793GluTrp: 0.793 ± 0.133
3.89GluTyr: 3.89 ± 0.278
0.0GluXaa: 0.0 ± 0.0
Phe
1.587PheAla: 1.587 ± 0.221
0.358PheCys: 0.358 ± 0.102
2.636PheAsp: 2.636 ± 0.293
2.917PheGlu: 2.917 ± 0.284
1.612PhePhe: 1.612 ± 0.211
2.303PheGly: 2.303 ± 0.34
1.126PheHis: 1.126 ± 0.199
3.02PheIle: 3.02 ± 0.307
2.866PheLys: 2.866 ± 0.302
3.403PheLeu: 3.403 ± 0.289
0.998PheMet: 0.998 ± 0.169
2.661PheAsn: 2.661 ± 0.286
1.407PhePro: 1.407 ± 0.198
1.356PheGln: 1.356 ± 0.211
1.945PheArg: 1.945 ± 0.174
3.352PheSer: 3.352 ± 0.342
2.764PheThr: 2.764 ± 0.3
2.277PheVal: 2.277 ± 0.266
0.256PheTrp: 0.256 ± 0.083
1.817PheTyr: 1.817 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
2.559GlyAla: 2.559 ± 0.311
0.614GlyCys: 0.614 ± 0.129
3.838GlyAsp: 3.838 ± 0.363
4.504GlyGlu: 4.504 ± 0.348
2.508GlyPhe: 2.508 ± 0.247
4.709GlyGly: 4.709 ± 0.832
1.382GlyHis: 1.382 ± 0.163
4.35GlyIle: 4.35 ± 0.395
3.89GlyLys: 3.89 ± 0.267
4.734GlyLeu: 4.734 ± 0.445
1.663GlyMet: 1.663 ± 0.31
3.327GlyAsn: 3.327 ± 0.331
0.665GlyPro: 0.665 ± 0.144
1.842GlyGln: 1.842 ± 0.239
2.687GlyArg: 2.687 ± 0.278
4.12GlySer: 4.12 ± 0.422
3.659GlyThr: 3.659 ± 0.413
5.323GlyVal: 5.323 ± 0.38
0.844GlyTrp: 0.844 ± 0.16
2.61GlyTyr: 2.61 ± 0.266
0.0GlyXaa: 0.0 ± 0.0
His
0.793HisAla: 0.793 ± 0.127
0.179HisCys: 0.179 ± 0.075
0.972HisAsp: 0.972 ± 0.183
1.049HisGlu: 1.049 ± 0.143
0.691HisPhe: 0.691 ± 0.137
1.228HisGly: 1.228 ± 0.175
0.409HisHis: 0.409 ± 0.103
1.689HisIle: 1.689 ± 0.208
1.663HisLys: 1.663 ± 0.21
2.15HisLeu: 2.15 ± 0.277
0.409HisMet: 0.409 ± 0.107
1.535HisAsn: 1.535 ± 0.21
0.691HisPro: 0.691 ± 0.13
0.691HisGln: 0.691 ± 0.151
1.126HisArg: 1.126 ± 0.174
1.279HisSer: 1.279 ± 0.167
1.407HisThr: 1.407 ± 0.198
1.612HisVal: 1.612 ± 0.184
0.333HisTrp: 0.333 ± 0.091
0.87HisTyr: 0.87 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
4.018IleAla: 4.018 ± 0.351
0.512IleCys: 0.512 ± 0.135
5.297IleAsp: 5.297 ± 0.367
6.423IleGlu: 6.423 ± 0.425
1.868IlePhe: 1.868 ± 0.204
3.506IleGly: 3.506 ± 0.344
1.228IleHis: 1.228 ± 0.196
4.018IleIle: 4.018 ± 0.3
5.476IleLys: 5.476 ± 0.445
4.785IleLeu: 4.785 ± 0.407
1.587IleMet: 1.587 ± 0.197
4.146IleAsn: 4.146 ± 0.457
2.354IlePro: 2.354 ± 0.333
2.354IleGln: 2.354 ± 0.228
3.429IleArg: 3.429 ± 0.319
4.99IleSer: 4.99 ± 0.379
3.966IleThr: 3.966 ± 0.362
4.427IleVal: 4.427 ± 0.319
0.256IleTrp: 0.256 ± 0.088
2.38IleTyr: 2.38 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
3.378LysAla: 3.378 ± 0.327
0.486LysCys: 0.486 ± 0.134
4.888LysAsp: 4.888 ± 0.481
7.728LysGlu: 7.728 ± 0.637
2.61LysPhe: 2.61 ± 0.292
4.325LysGly: 4.325 ± 0.359
1.689LysHis: 1.689 ± 0.193
4.325LysIle: 4.325 ± 0.325
5.886LysLys: 5.886 ± 0.623
6.244LysLeu: 6.244 ± 0.509
2.175LysMet: 2.175 ± 0.245
4.197LysAsn: 4.197 ± 0.356
1.894LysPro: 1.894 ± 0.297
3.48LysGln: 3.48 ± 0.308
3.276LysArg: 3.276 ± 0.309
4.478LysSer: 4.478 ± 0.427
3.915LysThr: 3.915 ± 0.3
4.939LysVal: 4.939 ± 0.417
0.972LysTrp: 0.972 ± 0.147
3.045LysTyr: 3.045 ± 0.303
0.0LysXaa: 0.0 ± 0.0
Leu
4.094LeuAla: 4.094 ± 0.324
0.742LeuCys: 0.742 ± 0.143
6.295LeuAsp: 6.295 ± 0.407
7.958LeuGlu: 7.958 ± 0.456
3.199LeuPhe: 3.199 ± 0.219
4.99LeuGly: 4.99 ± 0.41
1.612LeuHis: 1.612 ± 0.204
5.655LeuIle: 5.655 ± 0.481
6.705LeuLys: 6.705 ± 0.572
5.63LeuLeu: 5.63 ± 0.461
1.561LeuMet: 1.561 ± 0.217
4.657LeuAsn: 4.657 ± 0.387
3.506LeuPro: 3.506 ± 0.294
3.787LeuGln: 3.787 ± 0.283
4.427LeuArg: 4.427 ± 0.368
6.577LeuSer: 6.577 ± 0.455
5.195LeuThr: 5.195 ± 0.341
5.195LeuVal: 5.195 ± 0.411
0.512LeuTrp: 0.512 ± 0.12
3.403LeuTyr: 3.403 ± 0.316
0.0LeuXaa: 0.0 ± 0.0
Met
1.049MetAla: 1.049 ± 0.185
0.128MetCys: 0.128 ± 0.062
1.663MetAsp: 1.663 ± 0.196
1.894MetGlu: 1.894 ± 0.208
0.87MetPhe: 0.87 ± 0.135
1.228MetGly: 1.228 ± 0.235
0.409MetHis: 0.409 ± 0.095
1.433MetIle: 1.433 ± 0.252
1.868MetLys: 1.868 ± 0.217
1.715MetLeu: 1.715 ± 0.217
0.435MetMet: 0.435 ± 0.094
1.587MetAsn: 1.587 ± 0.241
0.64MetPro: 0.64 ± 0.148
0.691MetGln: 0.691 ± 0.115
1.407MetArg: 1.407 ± 0.246
2.047MetSer: 2.047 ± 0.196
1.279MetThr: 1.279 ± 0.184
1.382MetVal: 1.382 ± 0.169
0.102MetTrp: 0.102 ± 0.065
0.921MetTyr: 0.921 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
2.738AsnAla: 2.738 ± 0.266
0.23AsnCys: 0.23 ± 0.075
3.557AsnAsp: 3.557 ± 0.272
3.403AsnGlu: 3.403 ± 0.332
2.098AsnPhe: 2.098 ± 0.215
3.403AsnGly: 3.403 ± 0.439
1.203AsnHis: 1.203 ± 0.179
3.762AsnIle: 3.762 ± 0.315
3.813AsnLys: 3.813 ± 0.352
5.118AsnLeu: 5.118 ± 0.377
1.254AsnMet: 1.254 ± 0.191
3.659AsnAsn: 3.659 ± 0.348
2.789AsnPro: 2.789 ± 0.346
2.124AsnGln: 2.124 ± 0.234
2.866AsnArg: 2.866 ± 0.29
3.89AsnSer: 3.89 ± 0.35
3.455AsnThr: 3.455 ± 0.376
4.453AsnVal: 4.453 ± 0.506
0.384AsnTrp: 0.384 ± 0.094
2.84AsnTyr: 2.84 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
1.177ProAla: 1.177 ± 0.215
0.077ProCys: 0.077 ± 0.044
2.405ProAsp: 2.405 ± 0.27
2.508ProGlu: 2.508 ± 0.21
1.484ProPhe: 1.484 ± 0.219
0.665ProGly: 0.665 ± 0.141
1.279ProHis: 1.279 ± 0.279
2.022ProIle: 2.022 ± 0.226
2.405ProLys: 2.405 ± 0.26
2.457ProLeu: 2.457 ± 0.243
0.589ProMet: 0.589 ± 0.131
2.098ProAsn: 2.098 ± 0.23
1.1ProPro: 1.1 ± 0.21
1.459ProGln: 1.459 ± 0.308
1.279ProArg: 1.279 ± 0.16
3.173ProSer: 3.173 ± 0.307
2.226ProThr: 2.226 ± 0.303
2.277ProVal: 2.277 ± 0.25
0.179ProTrp: 0.179 ± 0.067
1.356ProTyr: 1.356 ± 0.19
0.0ProXaa: 0.0 ± 0.0
Gln
2.38GlnAla: 2.38 ± 0.346
0.179GlnCys: 0.179 ± 0.064
1.945GlnAsp: 1.945 ± 0.209
3.762GlnGlu: 3.762 ± 0.274
1.791GlnPhe: 1.791 ± 0.216
2.175GlnGly: 2.175 ± 0.282
0.717GlnHis: 0.717 ± 0.161
2.508GlnIle: 2.508 ± 0.27
2.789GlnLys: 2.789 ± 0.284
3.378GlnLeu: 3.378 ± 0.294
0.921GlnMet: 0.921 ± 0.158
2.022GlnAsn: 2.022 ± 0.222
1.254GlnPro: 1.254 ± 0.242
2.073GlnGln: 2.073 ± 0.392
1.638GlnArg: 1.638 ± 0.24
2.738GlnSer: 2.738 ± 0.284
1.612GlnThr: 1.612 ± 0.198
2.533GlnVal: 2.533 ± 0.288
0.461GlnTrp: 0.461 ± 0.102
0.947GlnTyr: 0.947 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
2.303ArgAla: 2.303 ± 0.274
0.256ArgCys: 0.256 ± 0.086
3.173ArgAsp: 3.173 ± 0.289
3.813ArgGlu: 3.813 ± 0.353
2.457ArgPhe: 2.457 ± 0.244
3.276ArgGly: 3.276 ± 0.381
0.589ArgHis: 0.589 ± 0.12
3.173ArgIle: 3.173 ± 0.303
3.941ArgLys: 3.941 ± 0.354
4.478ArgLeu: 4.478 ± 0.356
1.279ArgMet: 1.279 ± 0.175
2.585ArgAsn: 2.585 ± 0.26
1.228ArgPro: 1.228 ± 0.198
1.945ArgGln: 1.945 ± 0.216
2.508ArgArg: 2.508 ± 0.281
2.457ArgSer: 2.457 ± 0.23
2.917ArgThr: 2.917 ± 0.306
3.224ArgVal: 3.224 ± 0.362
0.64ArgTrp: 0.64 ± 0.119
2.252ArgTyr: 2.252 ± 0.244
0.0ArgXaa: 0.0 ± 0.0
Ser
3.071SerAla: 3.071 ± 0.361
0.512SerCys: 0.512 ± 0.103
4.325SerAsp: 4.325 ± 0.315
4.734SerGlu: 4.734 ± 0.389
3.02SerPhe: 3.02 ± 0.226
4.709SerGly: 4.709 ± 0.403
1.51SerHis: 1.51 ± 0.222
4.376SerIle: 4.376 ± 0.359
5.144SerLys: 5.144 ± 0.466
5.911SerLeu: 5.911 ± 0.346
1.766SerMet: 1.766 ± 0.199
4.274SerAsn: 4.274 ± 0.457
2.226SerPro: 2.226 ± 0.22
2.329SerGln: 2.329 ± 0.25
3.685SerArg: 3.685 ± 0.309
5.425SerSer: 5.425 ± 0.519
3.941SerThr: 3.941 ± 0.365
4.581SerVal: 4.581 ± 0.37
0.844SerTrp: 0.844 ± 0.131
2.892SerTyr: 2.892 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
2.559ThrAla: 2.559 ± 0.335
0.256ThrCys: 0.256 ± 0.083
3.378ThrAsp: 3.378 ± 0.333
4.325ThrGlu: 4.325 ± 0.304
2.252ThrPhe: 2.252 ± 0.232
3.966ThrGly: 3.966 ± 0.47
1.024ThrHis: 1.024 ± 0.198
4.146ThrIle: 4.146 ± 0.316
3.864ThrLys: 3.864 ± 0.443
5.272ThrLeu: 5.272 ± 0.371
1.075ThrMet: 1.075 ± 0.166
2.815ThrAsn: 2.815 ± 0.292
2.738ThrPro: 2.738 ± 0.313
2.201ThrGln: 2.201 ± 0.241
2.943ThrArg: 2.943 ± 0.275
3.608ThrSer: 3.608 ± 0.379
3.557ThrThr: 3.557 ± 0.298
4.709ThrVal: 4.709 ± 0.415
0.435ThrTrp: 0.435 ± 0.129
3.045ThrTyr: 3.045 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
3.327ValAla: 3.327 ± 0.31
0.486ValCys: 0.486 ± 0.119
4.99ValAsp: 4.99 ± 0.327
5.604ValGlu: 5.604 ± 0.427
3.455ValPhe: 3.455 ± 0.345
3.813ValGly: 3.813 ± 0.251
1.51ValHis: 1.51 ± 0.223
4.299ValIle: 4.299 ± 0.307
5.451ValLys: 5.451 ± 0.47
5.937ValLeu: 5.937 ± 0.457
1.305ValMet: 1.305 ± 0.194
3.813ValAsn: 3.813 ± 0.333
2.354ValPro: 2.354 ± 0.29
2.457ValGln: 2.457 ± 0.264
3.378ValArg: 3.378 ± 0.319
4.529ValSer: 4.529 ± 0.429
4.888ValThr: 4.888 ± 0.543
4.401ValVal: 4.401 ± 0.374
0.512ValTrp: 0.512 ± 0.133
2.968ValTyr: 2.968 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
0.333TrpAla: 0.333 ± 0.096
0.077TrpCys: 0.077 ± 0.046
0.921TrpAsp: 0.921 ± 0.16
0.793TrpGlu: 0.793 ± 0.129
0.461TrpPhe: 0.461 ± 0.111
0.691TrpGly: 0.691 ± 0.142
0.128TrpHis: 0.128 ± 0.049
0.614TrpIle: 0.614 ± 0.113
0.64TrpLys: 0.64 ± 0.143
0.844TrpLeu: 0.844 ± 0.153
0.051TrpMet: 0.051 ± 0.037
0.512TrpAsn: 0.512 ± 0.138
0.0TrpPro: 0.0 ± 0.0
0.358TrpGln: 0.358 ± 0.097
0.537TrpArg: 0.537 ± 0.101
0.844TrpSer: 0.844 ± 0.146
0.461TrpThr: 0.461 ± 0.091
0.793TrpVal: 0.793 ± 0.155
0.205TrpTrp: 0.205 ± 0.087
0.563TrpTyr: 0.563 ± 0.108
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.201TyrAla: 2.201 ± 0.224
0.461TyrCys: 0.461 ± 0.124
3.276TyrAsp: 3.276 ± 0.309
3.403TyrGlu: 3.403 ± 0.269
1.459TyrPhe: 1.459 ± 0.173
2.405TyrGly: 2.405 ± 0.273
0.844TyrHis: 0.844 ± 0.174
3.199TyrIle: 3.199 ± 0.302
2.866TyrLys: 2.866 ± 0.296
4.529TyrLeu: 4.529 ± 0.422
0.998TyrMet: 0.998 ± 0.137
2.585TyrAsn: 2.585 ± 0.223
1.587TyrPro: 1.587 ± 0.204
1.484TyrGln: 1.484 ± 0.177
2.047TyrArg: 2.047 ± 0.213
2.968TyrSer: 2.968 ± 0.297
2.636TyrThr: 2.636 ± 0.243
2.84TyrVal: 2.84 ± 0.286
0.563TyrTrp: 0.563 ± 0.143
2.226TyrTyr: 2.226 ± 0.297
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 177 proteins (39079 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski