Amino acid dipepetide frequency for Mus musculus (Mouse)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.578AlaAla: 6.578 ± 0.031	1.363AlaCys: 1.363 ± 0.009	2.91AlaAsp: 2.91 ± 0.013	4.732AlaGlu: 4.732 ± 0.022	2.643AlaPhe: 2.643 ± 0.014	4.464AlaGly: 4.464 ± 0.021	1.561AlaHis: 1.561 ± 0.008	2.767AlaIle: 2.767 ± 0.011	3.352AlaLys: 3.352 ± 0.018	7.068AlaLeu: 7.068 ± 0.029	1.54AlaMet: 1.54 ± 0.009	2.022AlaAsn: 2.022 ± 0.009	4.041AlaPro: 4.041 ± 0.022	3.275AlaGln: 3.275 ± 0.016	3.57AlaArg: 3.57 ± 0.016	5.991AlaSer: 5.991 ± 0.02	3.663AlaThr: 3.663 ± 0.019	4.683AlaVal: 4.683 ± 0.015	0.764AlaTrp: 0.764 ± 0.007	1.501AlaTyr: 1.501 ± 0.008	0.009AlaXaa: 0.009 ± 0.001
Cys	1.255CysAla: 1.255 ± 0.008	0.703CysCys: 0.703 ± 0.012	1.043CysAsp: 1.043 ± 0.009	1.276CysGlu: 1.276 ± 0.013	0.873CysPhe: 0.873 ± 0.008	1.722CysGly: 1.722 ± 0.015	0.678CysHis: 0.678 ± 0.006	0.951CysIle: 0.951 ± 0.007	1.171CysLys: 1.171 ± 0.01	2.173CysLeu: 2.173 ± 0.013	0.452CysMet: 0.452 ± 0.004	0.811CysAsn: 0.811 ± 0.007	1.358CysPro: 1.358 ± 0.013	1.093CysGln: 1.093 ± 0.009	1.271CysArg: 1.271 ± 0.008	2.07CysSer: 2.07 ± 0.011	1.114CysThr: 1.114 ± 0.008	1.322CysVal: 1.322 ± 0.009	0.287CysTrp: 0.287 ± 0.003	0.588CysTyr: 0.588 ± 0.005	0.004CysXaa: 0.004 ± 0.0
Asp	2.867AspAla: 2.867 ± 0.01	1.048AspCys: 1.048 ± 0.01	2.641AspAsp: 2.641 ± 0.015	3.36AspGlu: 3.36 ± 0.017	2.077AspPhe: 2.077 ± 0.011	3.285AspGly: 3.285 ± 0.02	1.134AspHis: 1.134 ± 0.008	2.571AspIle: 2.571 ± 0.01	2.508AspLys: 2.508 ± 0.013	4.964AspLeu: 4.964 ± 0.018	1.16AspMet: 1.16 ± 0.007	1.649AspAsn: 1.649 ± 0.011	2.94AspPro: 2.94 ± 0.012	1.843AspGln: 1.843 ± 0.009	2.495AspArg: 2.495 ± 0.011	4.346AspSer: 4.346 ± 0.017	2.554AspThr: 2.554 ± 0.011	3.028AspVal: 3.028 ± 0.016	0.607AspTrp: 0.607 ± 0.005	1.448AspTyr: 1.448 ± 0.008	0.006AspXaa: 0.006 ± 0.0
Glu	5.211GluAla: 5.211 ± 0.025	1.351GluCys: 1.351 ± 0.012	4.519GluAsp: 4.519 ± 0.018	7.901GluGlu: 7.901 ± 0.039	1.996GluPhe: 1.996 ± 0.01	4.122GluGly: 4.122 ± 0.018	1.518GluHis: 1.518 ± 0.008	2.994GluIle: 2.994 ± 0.017	5.257GluLys: 5.257 ± 0.025	6.45GluLeu: 6.45 ± 0.029	1.659GluMet: 1.659 ± 0.009	2.986GluAsn: 2.986 ± 0.014	3.33GluPro: 3.33 ± 0.024	3.207GluGln: 3.207 ± 0.019	3.985GluArg: 3.985 ± 0.021	4.495GluSer: 4.495 ± 0.019	3.447GluThr: 3.447 ± 0.013	4.156GluVal: 4.156 ± 0.019	0.701GluTrp: 0.701 ± 0.005	1.563GluTyr: 1.563 ± 0.015	0.008GluXaa: 0.008 ± 0.001
Phe	1.942PheAla: 1.942 ± 0.011	0.992PheCys: 0.992 ± 0.007	1.623PheAsp: 1.623 ± 0.009	1.93PheGlu: 1.93 ± 0.009	1.644PhePhe: 1.644 ± 0.01	2.14PheGly: 2.14 ± 0.012	1.043PheHis: 1.043 ± 0.007	1.872PheIle: 1.872 ± 0.01	1.725PheLys: 1.725 ± 0.01	4.184PheLeu: 4.184 ± 0.021	0.791PheMet: 0.791 ± 0.007	1.302PheAsn: 1.302 ± 0.008	2.006PhePro: 2.006 ± 0.01	1.789PheGln: 1.789 ± 0.009	1.945PheArg: 1.945 ± 0.012	3.408PheSer: 3.408 ± 0.017	2.053PheThr: 2.053 ± 0.011	2.162PheVal: 2.162 ± 0.011	0.504PheTrp: 0.504 ± 0.005	1.205PheTyr: 1.205 ± 0.009	0.006PheXaa: 0.006 ± 0.0
Gly	4.226GlyAla: 4.226 ± 0.02	1.301GlyCys: 1.301 ± 0.012	3.127GlyAsp: 3.127 ± 0.014	3.983GlyGlu: 3.983 ± 0.018	2.34GlyPhe: 2.34 ± 0.012	4.759GlyGly: 4.759 ± 0.027	1.694GlyHis: 1.694 ± 0.009	2.651GlyIle: 2.651 ± 0.011	3.683GlyLys: 3.683 ± 0.016	5.803GlyLeu: 5.803 ± 0.021	1.309GlyMet: 1.309 ± 0.009	2.333GlyAsn: 2.333 ± 0.01	4.084GlyPro: 4.084 ± 0.036	2.786GlyGln: 2.786 ± 0.017	3.642GlyArg: 3.642 ± 0.015	5.97GlySer: 5.97 ± 0.026	3.685GlyThr: 3.685 ± 0.018	3.475GlyVal: 3.475 ± 0.015	0.753GlyTrp: 0.753 ± 0.007	1.709GlyTyr: 1.709 ± 0.011	0.008GlyXaa: 0.008 ± 0.0
His	1.32HisAla: 1.32 ± 0.008	0.723HisCys: 0.723 ± 0.007	0.888HisAsp: 0.888 ± 0.006	1.346HisGlu: 1.346 ± 0.008	1.092HisPhe: 1.092 ± 0.006	1.564HisGly: 1.564 ± 0.01	0.919HisHis: 0.919 ± 0.008	1.254HisIle: 1.254 ± 0.007	1.276HisLys: 1.276 ± 0.008	2.933HisLeu: 2.933 ± 0.013	0.608HisMet: 0.608 ± 0.005	0.854HisAsn: 0.854 ± 0.006	1.655HisPro: 1.655 ± 0.01	1.313HisGln: 1.313 ± 0.01	1.623HisArg: 1.623 ± 0.01	2.407HisSer: 2.407 ± 0.013	1.563HisThr: 1.563 ± 0.01	1.483HisVal: 1.483 ± 0.008	0.345HisTrp: 0.345 ± 0.004	0.802HisTyr: 0.802 ± 0.005	0.005HisXaa: 0.005 ± 0.0
Ile	2.513IleAla: 2.513 ± 0.011	1.084IleCys: 1.084 ± 0.007	1.938IleAsp: 1.938 ± 0.011	2.513IleGlu: 2.513 ± 0.013	1.909IlePhe: 1.909 ± 0.012	2.162IleGly: 2.162 ± 0.011	1.35IleHis: 1.35 ± 0.01	2.344IleIle: 2.344 ± 0.013	2.509IleLys: 2.509 ± 0.015	4.646IleLeu: 4.646 ± 0.016	1.003IleMet: 1.003 ± 0.007	1.744IleAsn: 1.744 ± 0.01	2.626IlePro: 2.626 ± 0.012	2.247IleGln: 2.247 ± 0.01	2.293IleArg: 2.293 ± 0.011	3.714IleSer: 3.714 ± 0.014	2.538IleThr: 2.538 ± 0.02	2.498IleVal: 2.498 ± 0.013	0.509IleTrp: 0.509 ± 0.005	1.387IleTyr: 1.387 ± 0.008	0.006IleXaa: 0.006 ± 0.0
Lys	4.045LysAla: 4.045 ± 0.017	1.163LysCys: 1.163 ± 0.009	3.204LysAsp: 3.204 ± 0.017	4.983LysGlu: 4.983 ± 0.024	1.7LysPhe: 1.7 ± 0.009	3.207LysGly: 3.207 ± 0.019	1.376LysHis: 1.376 ± 0.009	2.615LysIle: 2.615 ± 0.013	4.552LysLys: 4.552 ± 0.027	5.071LysLeu: 5.071 ± 0.02	1.44LysMet: 1.44 ± 0.008	2.283LysAsn: 2.283 ± 0.01	3.167LysPro: 3.167 ± 0.021	2.593LysGln: 2.593 ± 0.016	3.322LysArg: 3.322 ± 0.015	3.887LysSer: 3.887 ± 0.017	3.075LysThr: 3.075 ± 0.013	3.464LysVal: 3.464 ± 0.022	0.589LysTrp: 0.589 ± 0.006	1.499LysTyr: 1.499 ± 0.01	0.009LysXaa: 0.009 ± 0.001
Leu	6.781LeuAla: 6.781 ± 0.024	2.232LeuCys: 2.232 ± 0.012	4.748LeuAsp: 4.748 ± 0.017	7.127LeuGlu: 7.127 ± 0.028	3.398LeuPhe: 3.398 ± 0.015	5.787LeuGly: 5.787 ± 0.023	2.768LeuHis: 2.768 ± 0.013	3.87LeuIle: 3.87 ± 0.016	5.788LeuLys: 5.788 ± 0.022	10.974LeuLeu: 10.974 ± 0.043	2.096LeuMet: 2.096 ± 0.011	3.415LeuAsn: 3.415 ± 0.015	5.969LeuPro: 5.969 ± 0.021	5.914LeuGln: 5.914 ± 0.029	6.006LeuArg: 6.006 ± 0.019	8.248LeuSer: 8.248 ± 0.022	5.128LeuThr: 5.128 ± 0.017	5.531LeuVal: 5.531 ± 0.018	1.143LeuTrp: 1.143 ± 0.008	2.526LeuTyr: 2.526 ± 0.011	0.018LeuXaa: 0.018 ± 0.001
Met	2.009MetAla: 2.009 ± 0.009	0.393MetCys: 0.393 ± 0.004	1.294MetAsp: 1.294 ± 0.008	1.905MetGlu: 1.905 ± 0.011	0.745MetPhe: 0.745 ± 0.006	1.288MetGly: 1.288 ± 0.009	0.466MetHis: 0.466 ± 0.004	0.852MetIle: 0.852 ± 0.007	1.462MetLys: 1.462 ± 0.008	2.053MetLeu: 2.053 ± 0.01	0.574MetMet: 0.574 ± 0.005	0.919MetAsn: 0.919 ± 0.006	1.103MetPro: 1.103 ± 0.009	0.934MetGln: 0.934 ± 0.007	1.04MetArg: 1.04 ± 0.007	1.722MetSer: 1.722 ± 0.009	1.177MetThr: 1.177 ± 0.007	1.444MetVal: 1.444 ± 0.007	0.241MetTrp: 0.241 ± 0.003	0.6MetTyr: 0.6 ± 0.005	0.004MetXaa: 0.004 ± 0.0
Asn	2.011AsnAla: 2.011 ± 0.01	0.817AsnCys: 0.817 ± 0.007	1.454AsnAsp: 1.454 ± 0.01	2.115AsnGlu: 2.115 ± 0.011	1.453AsnPhe: 1.453 ± 0.009	2.431AsnGly: 2.431 ± 0.014	0.937AsnHis: 0.937 ± 0.006	2.058AsnIle: 2.058 ± 0.012	2.109AsnLys: 2.109 ± 0.011	3.647AsnLeu: 3.647 ± 0.014	0.911AsnMet: 0.911 ± 0.006	1.441AsnAsn: 1.441 ± 0.008	2.172AsnPro: 2.172 ± 0.01	1.656AsnGln: 1.656 ± 0.011	1.842AsnArg: 1.842 ± 0.009	3.11AsnSer: 3.11 ± 0.013	1.939AsnThr: 1.939 ± 0.008	2.134AsnVal: 2.134 ± 0.01	0.447AsnTrp: 0.447 ± 0.004	1.107AsnTyr: 1.107 ± 0.007	0.005AsnXaa: 0.005 ± 0.0
Pro	4.771ProAla: 4.771 ± 0.023	1.17ProCys: 1.17 ± 0.011	2.759ProAsp: 2.759 ± 0.012	4.528ProGlu: 4.528 ± 0.023	1.873ProPhe: 1.873 ± 0.01	5.098ProGly: 5.098 ± 0.044	1.435ProHis: 1.435 ± 0.009	1.861ProIle: 1.861 ± 0.014	2.83ProLys: 2.83 ± 0.022	5.235ProLeu: 5.235 ± 0.016	1.145ProMet: 1.145 ± 0.006	1.827ProAsn: 1.827 ± 0.009	6.036ProPro: 6.036 ± 0.051	2.906ProGln: 2.906 ± 0.017	3.301ProArg: 3.301 ± 0.014	5.913ProSer: 5.913 ± 0.023	3.208ProThr: 3.208 ± 0.02	3.917ProVal: 3.917 ± 0.022	0.674ProTrp: 0.674 ± 0.006	1.505ProTyr: 1.505 ± 0.011	0.01ProXaa: 0.01 ± 0.001
Gln	3.553GlnAla: 3.553 ± 0.018	0.987GlnCys: 0.987 ± 0.01	2.377GlnAsp: 2.377 ± 0.01	3.958GlnGlu: 3.958 ± 0.02	1.359GlnPhe: 1.359 ± 0.008	2.866GlnGly: 2.866 ± 0.016	1.309GlnHis: 1.309 ± 0.008	1.944GlnIle: 1.944 ± 0.009	2.94GlnLys: 2.94 ± 0.015	4.805GlnLeu: 4.805 ± 0.022	1.146GlnMet: 1.146 ± 0.008	1.82GlnAsn: 1.82 ± 0.01	2.88GlnPro: 2.88 ± 0.016	3.276GlnGln: 3.276 ± 0.031	3.016GlnArg: 3.016 ± 0.015	3.315GlnSer: 3.315 ± 0.017	2.379GlnThr: 2.379 ± 0.01	2.875GlnVal: 2.875 ± 0.012	0.53GlnTrp: 0.53 ± 0.004	1.14GlnTyr: 1.14 ± 0.007	0.007GlnXaa: 0.007 ± 0.001
Arg	3.775ArgAla: 3.775 ± 0.014	1.2ArgCys: 1.2 ± 0.01	2.773ArgAsp: 2.773 ± 0.011	3.978ArgGlu: 3.978 ± 0.019	1.832ArgPhe: 1.832 ± 0.009	3.496ArgGly: 3.496 ± 0.017	1.528ArgHis: 1.528 ± 0.008	2.394ArgIle: 2.394 ± 0.011	3.686ArgLys: 3.686 ± 0.014	5.392ArgLeu: 5.392 ± 0.02	1.23ArgMet: 1.23 ± 0.007	2.145ArgAsn: 2.145 ± 0.01	3.179ArgPro: 3.179 ± 0.015	2.68ArgGln: 2.68 ± 0.015	4.349ArgArg: 4.349 ± 0.019	4.393ArgSer: 4.393 ± 0.021	2.853ArgThr: 2.853 ± 0.013	3.169ArgVal: 3.169 ± 0.014	0.682ArgTrp: 0.682 ± 0.006	1.421ArgTyr: 1.421 ± 0.007	0.011ArgXaa: 0.011 ± 0.001
Ser	5.476SerAla: 5.476 ± 0.018	1.941SerCys: 1.941 ± 0.013	3.958SerAsp: 3.958 ± 0.018	5.309SerGlu: 5.309 ± 0.019	3.079SerPhe: 3.079 ± 0.012	5.742SerGly: 5.742 ± 0.026	2.261SerHis: 2.261 ± 0.012	3.282SerIle: 3.282 ± 0.012	4.114SerLys: 4.114 ± 0.018	8.472SerLeu: 8.472 ± 0.027	1.697SerMet: 1.697 ± 0.009	2.716SerAsn: 2.716 ± 0.011	6.219SerPro: 6.219 ± 0.026	4.054SerGln: 4.054 ± 0.017	4.641SerArg: 4.641 ± 0.018	9.861SerSer: 9.861 ± 0.047	4.724SerThr: 4.724 ± 0.026	5.075SerVal: 5.075 ± 0.016	1.115SerTrp: 1.115 ± 0.008	2.108SerTyr: 2.108 ± 0.01	0.014SerXaa: 0.014 ± 0.001
Thr	3.81ThrAla: 3.81 ± 0.023	1.329ThrCys: 1.329 ± 0.01	2.477ThrAsp: 2.477 ± 0.012	3.637ThrGlu: 3.637 ± 0.014	2.125ThrPhe: 2.125 ± 0.012	3.579ThrGly: 3.579 ± 0.021	1.346ThrHis: 1.346 ± 0.009	2.394ThrIle: 2.394 ± 0.013	2.646ThrLys: 2.646 ± 0.015	5.448ThrLeu: 5.448 ± 0.019	1.176ThrMet: 1.176 ± 0.006	1.702ThrAsn: 1.702 ± 0.009	3.707ThrPro: 3.707 ± 0.022	2.392ThrGln: 2.392 ± 0.011	2.499ThrArg: 2.499 ± 0.01	4.85ThrSer: 4.85 ± 0.029	3.168ThrThr: 3.168 ± 0.059	3.978ThrVal: 3.978 ± 0.02	0.717ThrTrp: 0.717 ± 0.007	1.443ThrTyr: 1.443 ± 0.008	0.007ThrXaa: 0.007 ± 0.0
Val	4.222ValAla: 4.222 ± 0.014	1.489ValCys: 1.489 ± 0.009	2.899ValAsp: 2.899 ± 0.013	3.814ValGlu: 3.814 ± 0.02	2.444ValPhe: 2.444 ± 0.012	3.295ValGly: 3.295 ± 0.014	1.604ValHis: 1.604 ± 0.009	2.921ValIle: 2.921 ± 0.013	3.341ValLys: 3.341 ± 0.019	6.274ValLeu: 6.274 ± 0.02	1.349ValMet: 1.349 ± 0.008	2.212ValAsn: 2.212 ± 0.012	3.729ValPro: 3.729 ± 0.023	2.77ValGln: 2.77 ± 0.011	3.017ValArg: 3.017 ± 0.012	5.01ValSer: 5.01 ± 0.019	3.931ValThr: 3.931 ± 0.032	3.989ValVal: 3.989 ± 0.018	0.712ValTrp: 0.712 ± 0.006	1.631ValTyr: 1.631 ± 0.008	0.01ValXaa: 0.01 ± 0.001
Trp	0.769TrpAla: 0.769 ± 0.005	0.233TrpCys: 0.233 ± 0.003	0.633TrpAsp: 0.633 ± 0.006	0.796TrpGlu: 0.796 ± 0.006	0.442TrpPhe: 0.442 ± 0.005	0.668TrpGly: 0.668 ± 0.007	0.296TrpHis: 0.296 ± 0.004	0.563TrpIle: 0.563 ± 0.005	0.806TrpLys: 0.806 ± 0.005	1.221TrpLeu: 1.221 ± 0.008	0.318TrpMet: 0.318 ± 0.004	0.534TrpAsn: 0.534 ± 0.005	0.509TrpPro: 0.509 ± 0.005	0.525TrpGln: 0.525 ± 0.005	0.729TrpArg: 0.729 ± 0.005	0.895TrpSer: 0.895 ± 0.008	0.701TrpThr: 0.701 ± 0.006	0.712TrpVal: 0.712 ± 0.006	0.192TrpTrp: 0.192 ± 0.003	0.333TrpTyr: 0.333 ± 0.004	0.002TrpXaa: 0.002 ± 0.0
Tyr	1.351TyrAla: 1.351 ± 0.008	0.691TyrCys: 0.691 ± 0.006	1.258TyrAsp: 1.258 ± 0.008	1.653TyrGlu: 1.653 ± 0.009	1.236TyrPhe: 1.236 ± 0.008	1.637TyrGly: 1.637 ± 0.01	0.749TyrHis: 0.749 ± 0.006	1.371TyrIle: 1.371 ± 0.008	1.477TyrLys: 1.477 ± 0.014	2.64TyrLeu: 2.64 ± 0.012	0.617TyrMet: 0.617 ± 0.004	1.075TyrAsn: 1.075 ± 0.007	1.284TyrPro: 1.284 ± 0.008	1.223TyrGln: 1.223 ± 0.007	1.567TyrArg: 1.567 ± 0.008	2.243TyrSer: 2.243 ± 0.011	1.522TyrThr: 1.522 ± 0.011	1.56TyrVal: 1.56 ± 0.009	0.361TyrTrp: 0.361 ± 0.005	0.933TyrTyr: 0.933 ± 0.006	0.004TyrXaa: 0.004 ± 0.0
Xaa	0.011XaaAla: 0.011 ± 0.001	0.004XaaCys: 0.004 ± 0.0	0.008XaaAsp: 0.008 ± 0.001	0.013XaaGlu: 0.013 ± 0.001	0.006XaaPhe: 0.006 ± 0.0	0.011XaaGly: 0.011 ± 0.001	0.003XaaHis: 0.003 ± 0.0	0.006XaaIle: 0.006 ± 0.0	0.01XaaLys: 0.01 ± 0.001	0.013XaaLeu: 0.013 ± 0.001	0.003XaaMet: 0.003 ± 0.0	0.006XaaAsn: 0.006 ± 0.0	0.007XaaPro: 0.007 ± 0.0	0.006XaaGln: 0.006 ± 0.0	0.011XaaArg: 0.011 ± 0.001	0.013XaaSer: 0.013 ± 0.001	0.007XaaThr: 0.007 ± 0.0	0.008XaaVal: 0.008 ± 0.001	0.003XaaTrp: 0.003 ± 0.0	0.003XaaTyr: 0.003 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 63656 proteins (28559426 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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