Amino acid dipepetide frequency for Alicyclobacillus hesperidum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.764AlaAla: 10.764 ± 0.14	1.234AlaCys: 1.234 ± 0.042	5.317AlaAsp: 5.317 ± 0.08	5.929AlaGlu: 5.929 ± 0.085	3.891AlaPhe: 3.891 ± 0.075	7.695AlaGly: 7.695 ± 0.103	2.293AlaHis: 2.293 ± 0.056	6.584AlaIle: 6.584 ± 0.093	4.28AlaLys: 4.28 ± 0.083	10.599AlaLeu: 10.599 ± 0.131	3.106AlaMet: 3.106 ± 0.065	3.3AlaAsn: 3.3 ± 0.077	3.555AlaPro: 3.555 ± 0.079	4.104AlaGln: 4.104 ± 0.075	5.94AlaArg: 5.94 ± 0.1	5.905AlaSer: 5.905 ± 0.098	5.402AlaThr: 5.402 ± 0.095	8.413AlaVal: 8.413 ± 0.116	1.42AlaTrp: 1.42 ± 0.046	2.881AlaTyr: 2.881 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.952CysAla: 0.952 ± 0.035	0.121CysCys: 0.121 ± 0.014	0.632CysAsp: 0.632 ± 0.029	0.576CysGlu: 0.576 ± 0.028	0.338CysPhe: 0.338 ± 0.022	0.979CysGly: 0.979 ± 0.035	0.262CysHis: 0.262 ± 0.017	0.587CysIle: 0.587 ± 0.025	0.298CysLys: 0.298 ± 0.018	0.902CysLeu: 0.902 ± 0.037	0.241CysMet: 0.241 ± 0.016	0.247CysAsn: 0.247 ± 0.019	0.464CysPro: 0.464 ± 0.025	0.29CysGln: 0.29 ± 0.017	0.594CysArg: 0.594 ± 0.031	0.499CysSer: 0.499 ± 0.026	0.55CysThr: 0.55 ± 0.028	0.759CysVal: 0.759 ± 0.029	0.108CysTrp: 0.108 ± 0.011	0.254CysTyr: 0.254 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	5.316AspAla: 5.316 ± 0.084	0.425AspCys: 0.425 ± 0.023	2.681AspAsp: 2.681 ± 0.061	4.015AspGlu: 4.015 ± 0.079	1.827AspPhe: 1.827 ± 0.049	4.212AspGly: 4.212 ± 0.08	1.094AspHis: 1.094 ± 0.035	3.237AspIle: 3.237 ± 0.064	1.766AspLys: 1.766 ± 0.043	4.929AspLeu: 4.929 ± 0.082	1.365AspMet: 1.365 ± 0.041	1.271AspAsn: 1.271 ± 0.044	2.68AspPro: 2.68 ± 0.056	1.378AspGln: 1.378 ± 0.046	3.074AspArg: 3.074 ± 0.073	2.082AspSer: 2.082 ± 0.043	2.553AspThr: 2.553 ± 0.056	5.204AspVal: 5.204 ± 0.083	0.846AspTrp: 0.846 ± 0.034	1.402AspTyr: 1.402 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.138GluAla: 6.138 ± 0.101	0.444GluCys: 0.444 ± 0.025	2.721GluAsp: 2.721 ± 0.059	3.732GluGlu: 3.732 ± 0.084	1.723GluPhe: 1.723 ± 0.045	3.501GluGly: 3.501 ± 0.066	1.66GluHis: 1.66 ± 0.049	3.58GluIle: 3.58 ± 0.066	2.121GluLys: 2.121 ± 0.06	6.117GluLeu: 6.117 ± 0.097	1.666GluMet: 1.666 ± 0.044	1.573GluAsn: 1.573 ± 0.041	2.301GluPro: 2.301 ± 0.058	3.234GluGln: 3.234 ± 0.072	5.337GluArg: 5.337 ± 0.104	2.991GluSer: 2.991 ± 0.064	2.998GluThr: 2.998 ± 0.065	4.402GluVal: 4.402 ± 0.088	0.949GluTrp: 0.949 ± 0.035	1.44GluTyr: 1.44 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	4.41PheAla: 4.41 ± 0.077	0.388PheCys: 0.388 ± 0.02	2.105PheAsp: 2.105 ± 0.051	1.788PheGlu: 1.788 ± 0.044	1.454PhePhe: 1.454 ± 0.05	3.434PheGly: 3.434 ± 0.074	1.021PheHis: 1.021 ± 0.036	2.036PheIle: 2.036 ± 0.056	0.792PheLys: 0.792 ± 0.03	3.449PheLeu: 3.449 ± 0.073	0.831PheMet: 0.831 ± 0.031	0.942PheAsn: 0.942 ± 0.033	1.571PhePro: 1.571 ± 0.044	1.352PheGln: 1.352 ± 0.043	2.242PheArg: 2.242 ± 0.051	2.184PheSer: 2.184 ± 0.057	2.146PheThr: 2.146 ± 0.054	3.439PheVal: 3.439 ± 0.075	0.53PheTrp: 0.53 ± 0.026	1.091PheTyr: 1.091 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	6.925GlyAla: 6.925 ± 0.112	0.876GlyCys: 0.876 ± 0.037	3.692GlyAsp: 3.692 ± 0.076	4.327GlyGlu: 4.327 ± 0.076	3.093GlyPhe: 3.093 ± 0.063	5.909GlyGly: 5.909 ± 0.1	1.846GlyHis: 1.846 ± 0.047	5.159GlyIle: 5.159 ± 0.082	3.28GlyLys: 3.28 ± 0.063	7.492GlyLeu: 7.492 ± 0.103	2.272GlyMet: 2.272 ± 0.056	2.246GlyAsn: 2.246 ± 0.073	2.449GlyPro: 2.449 ± 0.056	3.103GlyGln: 3.103 ± 0.074	4.693GlyArg: 4.693 ± 0.082	4.362GlySer: 4.362 ± 0.083	4.576GlyThr: 4.576 ± 0.1	6.822GlyVal: 6.822 ± 0.103	1.244GlyTrp: 1.244 ± 0.038	2.784GlyTyr: 2.784 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.455HisAla: 2.455 ± 0.064	0.243HisCys: 0.243 ± 0.021	1.178HisAsp: 1.178 ± 0.042	1.41HisGlu: 1.41 ± 0.041	0.919HisPhe: 0.919 ± 0.032	2.081HisGly: 2.081 ± 0.049	0.781HisHis: 0.781 ± 0.032	1.466HisIle: 1.466 ± 0.044	0.719HisLys: 0.719 ± 0.03	2.494HisLeu: 2.494 ± 0.056	0.659HisMet: 0.659 ± 0.032	0.666HisAsn: 0.666 ± 0.028	1.608HisPro: 1.608 ± 0.049	0.855HisGln: 0.855 ± 0.031	1.687HisArg: 1.687 ± 0.046	1.177HisSer: 1.177 ± 0.036	1.236HisThr: 1.236 ± 0.04	2.276HisVal: 2.276 ± 0.054	0.354HisTrp: 0.354 ± 0.02	0.672HisTyr: 0.672 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	7.092IleAla: 7.092 ± 0.097	0.641IleCys: 0.641 ± 0.026	3.601IleAsp: 3.601 ± 0.069	3.862IleGlu: 3.862 ± 0.075	1.635IlePhe: 1.635 ± 0.052	5.195IleGly: 5.195 ± 0.1	1.413IleHis: 1.413 ± 0.042	2.445IleIle: 2.445 ± 0.058	1.284IleLys: 1.284 ± 0.04	5.156IleLeu: 5.156 ± 0.099	1.019IleMet: 1.019 ± 0.034	1.318IleAsn: 1.318 ± 0.043	2.943IlePro: 2.943 ± 0.069	2.109IleGln: 2.109 ± 0.057	3.812IleArg: 3.812 ± 0.078	3.137IleSer: 3.137 ± 0.073	2.771IleThr: 2.771 ± 0.06	5.598IleVal: 5.598 ± 0.089	0.727IleTrp: 0.727 ± 0.026	1.5IleTyr: 1.5 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	3.402LysAla: 3.402 ± 0.067	0.204LysCys: 0.204 ± 0.016	1.553LysAsp: 1.553 ± 0.043	1.971LysGlu: 1.971 ± 0.051	0.928LysPhe: 0.928 ± 0.032	2.272LysGly: 2.272 ± 0.042	0.88LysHis: 0.88 ± 0.033	1.594LysIle: 1.594 ± 0.046	1.275LysLys: 1.275 ± 0.049	3.313LysLeu: 3.313 ± 0.069	0.867LysMet: 0.867 ± 0.033	0.949LysAsn: 0.949 ± 0.034	1.837LysPro: 1.837 ± 0.047	1.562LysGln: 1.562 ± 0.036	2.829LysArg: 2.829 ± 0.06	2.052LysSer: 2.052 ± 0.053	1.967LysThr: 1.967 ± 0.054	2.607LysVal: 2.607 ± 0.062	0.598LysTrp: 0.598 ± 0.03	0.982LysTyr: 0.982 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	11.6LeuAla: 11.6 ± 0.155	1.111LeuCys: 1.111 ± 0.037	4.844LeuAsp: 4.844 ± 0.08	5.177LeuGlu: 5.177 ± 0.093	3.934LeuPhe: 3.934 ± 0.085	7.476LeuGly: 7.476 ± 0.105	2.644LeuHis: 2.644 ± 0.064	4.785LeuIle: 4.785 ± 0.092	2.881LeuLys: 2.881 ± 0.063	9.652LeuLeu: 9.652 ± 0.126	2.33LeuMet: 2.33 ± 0.057	2.731LeuAsn: 2.731 ± 0.052	4.935LeuPro: 4.935 ± 0.083	4.367LeuGln: 4.367 ± 0.08	6.796LeuArg: 6.796 ± 0.107	6.129LeuSer: 6.129 ± 0.084	5.656LeuThr: 5.656 ± 0.096	7.704LeuVal: 7.704 ± 0.107	1.259LeuTrp: 1.259 ± 0.037	2.877LeuTyr: 2.877 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.802MetAla: 2.802 ± 0.056	0.197MetCys: 0.197 ± 0.016	1.285MetAsp: 1.285 ± 0.036	1.501MetGlu: 1.501 ± 0.04	0.959MetPhe: 0.959 ± 0.038	1.881MetGly: 1.881 ± 0.054	0.637MetHis: 0.637 ± 0.026	1.343MetIle: 1.343 ± 0.04	1.046MetLys: 1.046 ± 0.032	2.492MetLeu: 2.492 ± 0.054	0.806MetMet: 0.806 ± 0.031	1.002MetAsn: 1.002 ± 0.035	1.263MetPro: 1.263 ± 0.032	1.187MetGln: 1.187 ± 0.038	1.83MetArg: 1.83 ± 0.045	1.712MetSer: 1.712 ± 0.046	1.638MetThr: 1.638 ± 0.043	1.893MetVal: 1.893 ± 0.049	0.298MetTrp: 0.298 ± 0.019	0.8MetTyr: 0.8 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	2.965AsnAla: 2.965 ± 0.056	0.24AsnCys: 0.24 ± 0.018	1.299AsnAsp: 1.299 ± 0.036	1.594AsnGlu: 1.594 ± 0.05	0.941AsnPhe: 0.941 ± 0.036	2.681AsnGly: 2.681 ± 0.075	0.699AsnHis: 0.699 ± 0.03	1.541AsnIle: 1.541 ± 0.044	0.885AsnLys: 0.885 ± 0.032	2.807AsnLeu: 2.807 ± 0.061	0.806AsnMet: 0.806 ± 0.028	0.915AsnAsn: 0.915 ± 0.042	1.957AsnPro: 1.957 ± 0.053	1.359AsnGln: 1.359 ± 0.045	1.924AsnArg: 1.924 ± 0.051	1.403AsnSer: 1.403 ± 0.046	1.517AsnThr: 1.517 ± 0.054	2.572AsnVal: 2.572 ± 0.054	0.51AsnTrp: 0.51 ± 0.027	0.821AsnTyr: 0.821 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	4.045ProAla: 4.045 ± 0.077	0.351ProCys: 0.351 ± 0.019	2.746ProAsp: 2.746 ± 0.054	2.923ProGlu: 2.923 ± 0.057	1.948ProPhe: 1.948 ± 0.048	3.627ProGly: 3.627 ± 0.082	1.151ProHis: 1.151 ± 0.034	2.691ProIle: 2.691 ± 0.044	1.574ProLys: 1.574 ± 0.047	4.219ProLeu: 4.219 ± 0.076	1.169ProMet: 1.169 ± 0.038	1.685ProAsn: 1.685 ± 0.044	1.629ProPro: 1.629 ± 0.047	1.664ProGln: 1.664 ± 0.046	2.175ProArg: 2.175 ± 0.05	3.065ProSer: 3.065 ± 0.073	2.609ProThr: 2.609 ± 0.063	3.965ProVal: 3.965 ± 0.082	0.653ProTrp: 0.653 ± 0.026	1.566ProTyr: 1.566 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	4.684GlnAla: 4.684 ± 0.084	0.28GlnCys: 0.28 ± 0.018	1.647GlnAsp: 1.647 ± 0.044	2.118GlnGlu: 2.118 ± 0.056	1.476GlnPhe: 1.476 ± 0.041	2.679GlnGly: 2.679 ± 0.056	0.954GlnHis: 0.954 ± 0.037	2.34GlnIle: 2.34 ± 0.054	1.375GlnLys: 1.375 ± 0.042	4.28GlnLeu: 4.28 ± 0.078	1.261GlnMet: 1.261 ± 0.044	1.261GlnAsn: 1.261 ± 0.041	1.918GlnPro: 1.918 ± 0.051	2.015GlnGln: 2.015 ± 0.068	2.593GlnArg: 2.593 ± 0.056	2.569GlnSer: 2.569 ± 0.063	2.442GlnThr: 2.442 ± 0.055	3.432GlnVal: 3.432 ± 0.063	0.532GlnTrp: 0.532 ± 0.025	1.212GlnTyr: 1.212 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	5.76ArgAla: 5.76 ± 0.093	0.533ArgCys: 0.533 ± 0.027	3.54ArgAsp: 3.54 ± 0.078	4.509ArgGlu: 4.509 ± 0.089	2.447ArgPhe: 2.447 ± 0.056	4.08ArgGly: 4.08 ± 0.072	1.644ArgHis: 1.644 ± 0.05	3.866ArgIle: 3.866 ± 0.061	2.653ArgLys: 2.653 ± 0.062	6.864ArgLeu: 6.864 ± 0.106	2.027ArgMet: 2.027 ± 0.047	1.854ArgAsn: 1.854 ± 0.051	2.862ArgPro: 2.862 ± 0.059	2.969ArgGln: 2.969 ± 0.065	5.102ArgArg: 5.102 ± 0.097	3.23ArgSer: 3.23 ± 0.065	3.373ArgThr: 3.373 ± 0.07	5.105ArgVal: 5.105 ± 0.082	0.96ArgTrp: 0.96 ± 0.037	1.968ArgTyr: 1.968 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	5.441SerAla: 5.441 ± 0.102	0.428SerCys: 0.428 ± 0.022	2.864SerAsp: 2.864 ± 0.065	3.084SerGlu: 3.084 ± 0.064	2.468SerPhe: 2.468 ± 0.057	5.046SerGly: 5.046 ± 0.099	1.353SerHis: 1.353 ± 0.043	3.393SerIle: 3.393 ± 0.067	1.768SerLys: 1.768 ± 0.047	5.772SerLeu: 5.772 ± 0.089	1.578SerMet: 1.578 ± 0.047	1.718SerAsn: 1.718 ± 0.054	2.602SerPro: 2.602 ± 0.058	2.146SerGln: 2.146 ± 0.056	3.192SerArg: 3.192 ± 0.066	3.506SerSer: 3.506 ± 0.097	3.231SerThr: 3.231 ± 0.075	4.702SerVal: 4.702 ± 0.075	0.846SerTrp: 0.846 ± 0.032	1.723SerTyr: 1.723 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	5.406ThrAla: 5.406 ± 0.087	0.5ThrCys: 0.5 ± 0.025	2.663ThrAsp: 2.663 ± 0.057	2.622ThrGlu: 2.622 ± 0.06	2.292ThrPhe: 2.292 ± 0.064	4.783ThrGly: 4.783 ± 0.089	1.293ThrHis: 1.293 ± 0.04	3.056ThrIle: 3.056 ± 0.06	1.754ThrLys: 1.754 ± 0.046	5.489ThrLeu: 5.489 ± 0.09	1.342ThrMet: 1.342 ± 0.039	1.806ThrAsn: 1.806 ± 0.065	2.983ThrPro: 2.983 ± 0.067	1.839ThrGln: 1.839 ± 0.051	2.817ThrArg: 2.817 ± 0.064	3.542ThrSer: 3.542 ± 0.076	3.245ThrThr: 3.245 ± 0.072	4.937ThrVal: 4.937 ± 0.083	0.912ThrTrp: 0.912 ± 0.037	1.809ThrTyr: 1.809 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	8.549ValAla: 8.549 ± 0.111	1.02ValCys: 1.02 ± 0.036	4.683ValAsp: 4.683 ± 0.073	5.055ValGlu: 5.055 ± 0.091	3.143ValPhe: 3.143 ± 0.065	6.195ValGly: 6.195 ± 0.094	2.152ValHis: 2.152 ± 0.048	5.114ValIle: 5.114 ± 0.08	2.699ValLys: 2.699 ± 0.063	8.087ValLeu: 8.087 ± 0.117	2.054ValMet: 2.054 ± 0.053	2.525ValAsn: 2.525 ± 0.063	3.902ValPro: 3.902 ± 0.069	3.46ValGln: 3.46 ± 0.068	5.451ValArg: 5.451 ± 0.083	5.021ValSer: 5.021 ± 0.083	4.702ValThr: 4.702 ± 0.091	7.351ValVal: 7.351 ± 0.12	1.113ValTrp: 1.113 ± 0.038	2.395ValTyr: 2.395 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	1.034TrpAla: 1.034 ± 0.04	0.134TrpCys: 0.134 ± 0.014	0.633TrpAsp: 0.633 ± 0.031	0.606TrpGlu: 0.606 ± 0.025	0.653TrpPhe: 0.653 ± 0.029	0.869TrpGly: 0.869 ± 0.028	0.383TrpHis: 0.383 ± 0.021	0.92TrpIle: 0.92 ± 0.034	0.473TrpLys: 0.473 ± 0.024	1.906TrpLeu: 1.906 ± 0.052	0.513TrpMet: 0.513 ± 0.022	0.527TrpAsn: 0.527 ± 0.026	0.632TrpPro: 0.632 ± 0.03	0.769TrpGln: 0.769 ± 0.03	1.069TrpArg: 1.069 ± 0.04	0.934TrpSer: 0.934 ± 0.033	0.858TrpThr: 0.858 ± 0.031	0.909TrpVal: 0.909 ± 0.033	0.283TrpTrp: 0.283 ± 0.022	0.475TrpTyr: 0.475 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.869TyrAla: 2.869 ± 0.064	0.282TyrCys: 0.282 ± 0.021	1.672TyrAsp: 1.672 ± 0.045	1.829TyrGlu: 1.829 ± 0.05	1.135TyrPhe: 1.135 ± 0.037	2.56TyrGly: 2.56 ± 0.055	0.775TyrHis: 0.775 ± 0.029	1.523TyrIle: 1.523 ± 0.042	0.724TyrLys: 0.724 ± 0.03	2.871TyrLeu: 2.871 ± 0.062	0.66TyrMet: 0.66 ± 0.031	0.9TyrAsn: 0.9 ± 0.039	1.362TyrPro: 1.362 ± 0.044	1.305TyrGln: 1.305 ± 0.04	2.185TyrArg: 2.185 ± 0.052	1.36TyrSer: 1.36 ± 0.044	1.621TyrThr: 1.621 ± 0.042	2.581TyrVal: 2.581 ± 0.066	0.439TyrTrp: 0.439 ± 0.022	0.942TyrTyr: 0.942 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2843 proteins (851207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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