Blastococcus sp. DSM 46838

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2DZY8|A0A1I2DZY8_9ACTN Cation-transporting ATPase E OS=Blastococcus sp. DSM 46838 OX=1798228 GN=SAMN05216574_106186 PE=4 SV=1
MM1 pKa = 7.15RR2 pKa = 11.84TRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 10.46GSTGAAALAVALLAACSSGTTDD28 pKa = 4.26ASGGSDD34 pKa = 3.01GGASADD40 pKa = 3.62GPLAEE45 pKa = 4.78VVADD49 pKa = 3.59VDD51 pKa = 3.59AAAQARR57 pKa = 11.84DD58 pKa = 3.64TFAVPSEE65 pKa = 4.07PVDD68 pKa = 3.49VSGWEE73 pKa = 3.98GRR75 pKa = 11.84TVYY78 pKa = 9.85YY79 pKa = 10.73VPITAQISVFQVYY92 pKa = 9.87GEE94 pKa = 4.61KK95 pKa = 9.73IGEE98 pKa = 4.06ALEE101 pKa = 4.38TVGADD106 pKa = 4.38LQICDD111 pKa = 4.05GGSNPSQISGCIDD124 pKa = 3.28QAVGASAAAIITDD137 pKa = 3.5NVPYY141 pKa = 10.95GMAANALDD149 pKa = 4.07GARR152 pKa = 11.84SAGIPVLIANQLPDD166 pKa = 4.01PAHH169 pKa = 6.65PADD172 pKa = 3.74EE173 pKa = 4.14SLAYY177 pKa = 10.57VEE179 pKa = 5.81GPATEE184 pKa = 4.86MITSVADD191 pKa = 3.39WVVADD196 pKa = 4.31SDD198 pKa = 4.33GEE200 pKa = 4.34ATVVLQKK207 pKa = 9.83VTDD210 pKa = 3.96NPSTIAWAEE219 pKa = 3.85AAEE222 pKa = 3.91QRR224 pKa = 11.84IDD226 pKa = 4.17EE227 pKa = 4.62RR228 pKa = 11.84CPDD231 pKa = 3.49CTVVVNEE238 pKa = 4.19VSASNFPLIPSSTSSALLANPDD260 pKa = 3.16ARR262 pKa = 11.84YY263 pKa = 9.9VLAEE267 pKa = 4.26FEE269 pKa = 5.3HH270 pKa = 6.64FVQPTLGGIQQSPNAADD287 pKa = 3.31IKK289 pKa = 10.46IVASAATLSGLQMLADD305 pKa = 5.03GAQLHH310 pKa = 6.98ADD312 pKa = 3.67AGQNFAFQGWATADD326 pKa = 3.2ALFRR330 pKa = 11.84LVAGQEE336 pKa = 3.88IPEE339 pKa = 3.99YY340 pKa = 10.23DD341 pKa = 3.34YY342 pKa = 11.78SFRR345 pKa = 11.84LFTQDD350 pKa = 3.66NVADD354 pKa = 4.91LDD356 pKa = 4.69LSDD359 pKa = 4.85EE360 pKa = 4.51GQASGSWYY368 pKa = 10.58GPTDD372 pKa = 3.59YY373 pKa = 11.38ADD375 pKa = 3.45EE376 pKa = 4.66FAALWVEE383 pKa = 4.55GG384 pKa = 3.93

Molecular weight: 39.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1VWJ0|A0A1I1VWJ0_9ACTN o-succinylbenzoate synthase OS=Blastococcus sp. DSM 46838 OX=1798228 GN=menC PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4797

0

4797

1512024

19

1967

315.2

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.303 ± 0.062

0.687 ± 0.009

6.276 ± 0.03

5.626 ± 0.033

2.589 ± 0.02

9.697 ± 0.035

2.013 ± 0.014

2.978 ± 0.025

1.381 ± 0.022

10.699 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.663 ± 0.014

1.444 ± 0.018

6.234 ± 0.031

2.701 ± 0.017

8.192 ± 0.036

4.846 ± 0.023

5.864 ± 0.024

9.605 ± 0.038

1.47 ± 0.015

1.734 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski