Lachnospiraceae bacterium KHCPX20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2W9K9|A0A1H2W9K9_9FIRM Putative hydrolase of the HAD superfamily OS=Lachnospiraceae bacterium KHCPX20 OX=1855375 GN=SAMN05216391_1171 PE=4 SV=1
MM1 pKa = 7.54TDD3 pKa = 3.08ADD5 pKa = 3.98RR6 pKa = 11.84AEE8 pKa = 4.0AFINGWYY15 pKa = 10.22VKK17 pKa = 10.3GSTGSSSGGMEE28 pKa = 4.69GGPGGNGGPGGNDD41 pKa = 3.28GPGGDD46 pKa = 4.01NAGGPPSGEE55 pKa = 4.28KK56 pKa = 10.4PNGAGPGGDD65 pKa = 3.34NAGGPHH71 pKa = 6.51AMNGGSSDD79 pKa = 3.76EE80 pKa = 4.34VGTPDD85 pKa = 4.72SGTTQSASSKK95 pKa = 9.15TDD97 pKa = 3.05SANYY101 pKa = 10.26SSFAEE106 pKa = 4.05MLAAYY111 pKa = 9.5QADD114 pKa = 3.37IAEE117 pKa = 4.26IQAGDD122 pKa = 3.51EE123 pKa = 4.15YY124 pKa = 11.33GNNIVDD130 pKa = 5.12LYY132 pKa = 11.59NPLNYY137 pKa = 9.56IGADD141 pKa = 3.6GTDD144 pKa = 3.29GPTWARR150 pKa = 11.84ILMGASEE157 pKa = 4.8GDD159 pKa = 3.02ISMMNSLNLQVAWLNSGTDD178 pKa = 5.57AEE180 pKa = 6.16IEE182 pKa = 4.18WQWNGGHH189 pKa = 5.84VPSEE193 pKa = 3.92IFGDD197 pKa = 4.07SLALYY202 pKa = 9.53VDD204 pKa = 3.96TMYY207 pKa = 11.43GEE209 pKa = 4.5YY210 pKa = 10.56VDD212 pKa = 4.39GAVKK216 pKa = 8.83ITKK219 pKa = 9.68AAASGQTTNGDD230 pKa = 3.29GTEE233 pKa = 4.09PSGTDD238 pKa = 3.04LSAWVSYY245 pKa = 11.07DD246 pKa = 3.18STSGVGMTLAGAAAYY261 pKa = 7.55RR262 pKa = 11.84TSGASKK268 pKa = 10.59AMPGFDD274 pKa = 3.49VMDD277 pKa = 4.28YY278 pKa = 10.69GQEE281 pKa = 3.98DD282 pKa = 5.17YY283 pKa = 11.81VFGSSTADD291 pKa = 2.98ARR293 pKa = 11.84HH294 pKa = 6.2WDD296 pKa = 3.75TYY298 pKa = 7.54VLKK301 pKa = 10.9VFEE304 pKa = 4.28EE305 pKa = 4.45HH306 pKa = 7.48ADD308 pKa = 3.62TLAEE312 pKa = 4.11LFNN315 pKa = 4.61

Molecular weight: 32.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2UP34|A0A1H2UP34_9FIRM Transcriptional regulator LacI family OS=Lachnospiraceae bacterium KHCPX20 OX=1855375 GN=SAMN05216391_11161 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.28GGRR28 pKa = 11.84KK29 pKa = 9.04VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2926

0

2926

953569

25

4544

325.9

36.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.443 ± 0.051

1.317 ± 0.019

6.185 ± 0.04

7.417 ± 0.056

3.94 ± 0.03

6.853 ± 0.04

1.873 ± 0.021

6.981 ± 0.044

7.302 ± 0.051

8.767 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.066 ± 0.032

4.364 ± 0.037

3.19 ± 0.025

3.27 ± 0.025

4.535 ± 0.036

6.081 ± 0.036

5.555 ± 0.044

6.826 ± 0.048

0.843 ± 0.016

4.192 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski