Lake Sarah-associated circular virus-43

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GAE9|A0A126GAE9_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-43 OX=1685772 PE=4 SV=1
MM1 pKa = 7.73QSRR4 pKa = 11.84CWCFTVNFKK13 pKa = 10.78YY14 pKa = 11.0LNDD17 pKa = 3.72YY18 pKa = 9.95EE19 pKa = 6.56AIDD22 pKa = 3.68PAEE25 pKa = 3.96WPGCVVAVWQHH36 pKa = 4.74EE37 pKa = 4.38VGEE40 pKa = 4.77GGTEE44 pKa = 3.85HH45 pKa = 6.57LQGYY49 pKa = 8.92VNFAKK54 pKa = 10.18PKK56 pKa = 10.48RR57 pKa = 11.84MGALHH62 pKa = 6.82KK63 pKa = 10.54LSGMARR69 pKa = 11.84ASVRR73 pKa = 11.84PRR75 pKa = 11.84AKK77 pKa = 10.59LATKK81 pKa = 10.14KK82 pKa = 10.81DD83 pKa = 3.4NLVYY87 pKa = 9.42CTKK90 pKa = 10.89EE91 pKa = 3.77EE92 pKa = 4.1GRR94 pKa = 11.84LDD96 pKa = 3.6GPFYY100 pKa = 10.7FPSKK104 pKa = 10.23EE105 pKa = 4.02KK106 pKa = 11.12VEE108 pKa = 4.57AYY110 pKa = 10.31CKK112 pKa = 10.55VEE114 pKa = 3.62NGQRR118 pKa = 11.84TDD120 pKa = 3.32LALISDD126 pKa = 3.77QVARR130 pKa = 11.84GLTDD134 pKa = 3.74KK135 pKa = 10.71EE136 pKa = 3.79IAEE139 pKa = 4.37INPIAILKK147 pKa = 6.63YY148 pKa = 10.35QKK150 pKa = 10.88GIDD153 pKa = 3.71HH154 pKa = 6.8LRR156 pKa = 11.84IAITDD161 pKa = 3.67TSRR164 pKa = 11.84LGDD167 pKa = 3.78EE168 pKa = 4.26VDD170 pKa = 3.38SMVFVGPTGTGKK182 pKa = 10.25SYY184 pKa = 11.05RR185 pKa = 11.84LNRR188 pKa = 11.84DD189 pKa = 3.25YY190 pKa = 11.4PEE192 pKa = 4.56GPEE195 pKa = 4.14WFWVSPGKK203 pKa = 9.83WFDD206 pKa = 3.69GYY208 pKa = 10.77QGQPGLVFDD217 pKa = 4.5EE218 pKa = 6.03FRR220 pKa = 11.84DD221 pKa = 3.2NWYY224 pKa = 9.35PYY226 pKa = 10.7QFLLRR231 pKa = 11.84LLDD234 pKa = 3.67TKK236 pKa = 10.4PYY238 pKa = 9.97RR239 pKa = 11.84VEE241 pKa = 3.91KK242 pKa = 10.69KK243 pKa = 10.41GGHH246 pKa = 5.98LSMRR250 pKa = 11.84AHH252 pKa = 7.65RR253 pKa = 11.84FRR255 pKa = 11.84FSTNIHH261 pKa = 5.82PMHH264 pKa = 7.46WYY266 pKa = 9.43QGVVKK271 pKa = 8.86PAWEE275 pKa = 3.9EE276 pKa = 4.17SPLKK280 pKa = 10.45RR281 pKa = 11.84RR282 pKa = 11.84LPYY285 pKa = 9.82IEE287 pKa = 5.27LMEE290 pKa = 4.23VPYY293 pKa = 10.68DD294 pKa = 3.72RR295 pKa = 11.84PTVSFDD301 pKa = 3.88SAAAWALLQPEE312 pKa = 4.61AVAPAARR319 pKa = 11.84AVYY322 pKa = 8.37GQRR325 pKa = 11.84MDD327 pKa = 3.2GG328 pKa = 3.41

Molecular weight: 37.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GAE9|A0A126GAE9_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-43 OX=1685772 PE=4 SV=1
MM1 pKa = 7.79PEE3 pKa = 4.12KK4 pKa = 10.39FLADD8 pKa = 4.74GISFHH13 pKa = 5.83HH14 pKa = 6.59HH15 pKa = 6.65KK16 pKa = 10.71KK17 pKa = 10.35LLCCAYY23 pKa = 9.68ILGINRR29 pKa = 11.84AKK31 pKa = 10.64GLRR34 pKa = 11.84IMSMYY39 pKa = 9.75YY40 pKa = 9.85RR41 pKa = 11.84KK42 pKa = 10.22KK43 pKa = 8.89KK44 pKa = 8.71TGGGGAARR52 pKa = 11.84GSVKK56 pKa = 10.42GYY58 pKa = 9.84AAAATRR64 pKa = 11.84VPGTAGKK71 pKa = 10.38AKK73 pKa = 10.26LAAARR78 pKa = 11.84NASKK82 pKa = 10.18SAQMARR88 pKa = 11.84YY89 pKa = 8.52VGRR92 pKa = 11.84RR93 pKa = 11.84SNVPGKK99 pKa = 10.08DD100 pKa = 2.7NHH102 pKa = 6.33TFDD105 pKa = 4.72PVVLNTNYY113 pKa = 10.07LQCDD117 pKa = 3.3NATAVAVSASGYY129 pKa = 6.97ITPGSSAIVLNQVSQGTSSTQRR151 pKa = 11.84IGRR154 pKa = 11.84KK155 pKa = 8.87ILMTAIHH162 pKa = 5.96IRR164 pKa = 11.84AYY166 pKa = 10.67LRR168 pKa = 11.84NTTASADD175 pKa = 3.61LNMVRR180 pKa = 11.84VALVYY185 pKa = 10.18IPEE188 pKa = 4.18MDD190 pKa = 3.25RR191 pKa = 11.84TTTTMPPQNAIWTSQDD207 pKa = 2.72ARR209 pKa = 11.84EE210 pKa = 4.5LRR212 pKa = 11.84VIDD215 pKa = 3.37NADD218 pKa = 3.49RR219 pKa = 11.84FKK221 pKa = 11.22VVRR224 pKa = 11.84QWTFMLLGDD233 pKa = 5.01ADD235 pKa = 3.86ACATGSEE242 pKa = 4.14AFAIDD247 pKa = 4.36EE248 pKa = 4.22YY249 pKa = 11.92VKK251 pKa = 11.14LNLPTCWLKK260 pKa = 11.49ANTDD264 pKa = 3.09GSFNSMEE271 pKa = 4.12KK272 pKa = 10.72GGLCLYY278 pKa = 9.72VQGSSALIPVLFPVIRR294 pKa = 11.84LYY296 pKa = 11.09FEE298 pKa = 4.54DD299 pKa = 3.5QQ300 pKa = 3.08

Molecular weight: 32.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

628

300

328

314.0

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.032 ± 1.511

1.752 ± 0.163

5.255 ± 0.386

4.618 ± 1.5

3.662 ± 0.435

7.803 ± 0.089

2.07 ± 0.265

4.299 ± 0.679

6.051 ± 0.252

7.803 ± 0.13

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.866 ± 0.307

3.822 ± 0.774

5.255 ± 1.043

3.503 ± 0.112

6.847 ± 0.118

5.414 ± 0.822

5.414 ± 1.041

6.847 ± 0.118

2.07 ± 0.703

4.618 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski