Synechococcus phage S-B28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Qadamvirus; Synechococcus virus SB28

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IFP0|A0A482IFP0_9CAUD Core protein OS=Synechococcus phage S-B28 OX=2545435 PE=4 SV=1
MM1 pKa = 7.32TATLSKK7 pKa = 10.29LTGQDD12 pKa = 3.71LIDD15 pKa = 3.89HH16 pKa = 6.24VKK18 pKa = 10.5LHH20 pKa = 6.79NDD22 pKa = 3.48CCSKK26 pKa = 11.24SEE28 pKa = 3.89MCLDD32 pKa = 3.44AGYY35 pKa = 10.93VNKK38 pKa = 10.67NGGPAFTDD46 pKa = 5.05FYY48 pKa = 10.98TALLEE53 pKa = 4.87AKK55 pKa = 9.35TDD57 pKa = 3.97LNQMPEE63 pKa = 4.1EE64 pKa = 4.4SQSGADD70 pKa = 3.03WYY72 pKa = 10.75DD73 pKa = 3.37NLSEE77 pKa = 4.18QDD79 pKa = 3.42QLLYY83 pKa = 10.95DD84 pKa = 4.41QIEE87 pKa = 4.39DD88 pKa = 3.69MCPEE92 pKa = 4.32FAKK95 pKa = 9.69LTAEE99 pKa = 4.14EE100 pKa = 4.44CQEE103 pKa = 4.3FMDD106 pKa = 4.42EE107 pKa = 4.8LSDD110 pKa = 4.33IGITTAQQFEE120 pKa = 4.45DD121 pKa = 4.05AYY123 pKa = 11.47YY124 pKa = 9.38MQFDD128 pKa = 3.78SYY130 pKa = 10.87KK131 pKa = 10.45AEE133 pKa = 3.85RR134 pKa = 11.84EE135 pKa = 3.93FAEE138 pKa = 4.48EE139 pKa = 4.53LMCQVTPNLEE149 pKa = 4.15DD150 pKa = 3.43SPVFFAIDD158 pKa = 3.33WQRR161 pKa = 11.84VWDD164 pKa = 4.2HH165 pKa = 5.98SLSYY169 pKa = 10.96DD170 pKa = 3.72FSTIEE175 pKa = 4.37FDD177 pKa = 2.95GTTYY181 pKa = 10.49FFHH184 pKa = 7.21NNN186 pKa = 2.92

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IFQ7|A0A482IFQ7_9CAUD Uncharacterized protein OS=Synechococcus phage S-B28 OX=2545435 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 10.22PIRR6 pKa = 11.84NSLGPLLLHH15 pKa = 6.7HH16 pKa = 6.68GCDD19 pKa = 3.07RR20 pKa = 11.84SLANLVLEE28 pKa = 5.34GKK30 pKa = 8.59TSPIPSEE37 pKa = 4.55NKK39 pKa = 10.04YY40 pKa = 10.86DD41 pKa = 3.46WLGPGIYY48 pKa = 9.7FWVDD52 pKa = 3.04SPSRR56 pKa = 11.84AMTWARR62 pKa = 11.84EE63 pKa = 4.0KK64 pKa = 11.14YY65 pKa = 10.02NRR67 pKa = 11.84EE68 pKa = 3.68TGSNLKK74 pKa = 10.46APQPKK79 pKa = 9.79GGPRR83 pKa = 11.84KK84 pKa = 9.73RR85 pKa = 11.84SFCARR90 pKa = 11.84MSGVKK95 pKa = 10.52GPMKK99 pKa = 10.2DD100 pKa = 3.05EE101 pKa = 4.46KK102 pKa = 11.11GRR104 pKa = 11.84PTRR107 pKa = 11.84KK108 pKa = 9.9ALALRR113 pKa = 11.84KK114 pKa = 8.38WKK116 pKa = 10.63CC117 pKa = 3.1

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

14035

40

1356

255.2

28.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.351 ± 0.421

0.955 ± 0.197

6.006 ± 0.263

6.398 ± 0.403

3.591 ± 0.178

6.975 ± 0.38

1.482 ± 0.2

5.33 ± 0.179

5.657 ± 0.383

8.272 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.174

5.33 ± 0.232

4.339 ± 0.195

4.973 ± 0.412

4.966 ± 0.261

6.79 ± 0.326

6.74 ± 0.492

6.227 ± 0.337

1.247 ± 0.124

3.862 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski