Peptoclostridium acidaminophilum DSM 3953

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptoclostridium; Peptoclostridium acidaminophilum

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8T706|W8T706_PEPAC Elongation factor Ts OS=Peptoclostridium acidaminophilum DSM 3953 OX=1286171 GN=tsf PE=3 SV=1
MM1 pKa = 7.35IAKK4 pKa = 9.76KK5 pKa = 10.06AARR8 pKa = 11.84YY9 pKa = 9.02LLAITICIVCFGSSIFASSNDD30 pKa = 3.35NPNAEE35 pKa = 4.43KK36 pKa = 10.82LKK38 pKa = 10.73ALKK41 pKa = 8.64KK42 pKa = 8.13TQATEE47 pKa = 4.03EE48 pKa = 4.33KK49 pKa = 10.29LGEE52 pKa = 4.33YY53 pKa = 10.14YY54 pKa = 10.77DD55 pKa = 4.3SLDD58 pKa = 3.38TGSAQDD64 pKa = 3.27IAVGVSTEE72 pKa = 4.64DD73 pKa = 3.66IIAAYY78 pKa = 10.51SNSTYY83 pKa = 10.35RR84 pKa = 11.84INIQEE89 pKa = 4.1SGFYY93 pKa = 10.44HH94 pKa = 7.04IDD96 pKa = 3.31LGLTEE101 pKa = 5.17DD102 pKa = 3.96ADD104 pKa = 4.44LFVEE108 pKa = 5.07TYY110 pKa = 10.54DD111 pKa = 3.81EE112 pKa = 4.14SLEE115 pKa = 4.06YY116 pKa = 10.74VDD118 pKa = 4.23WGSMVYY124 pKa = 10.4LQGNADD130 pKa = 3.62YY131 pKa = 11.02YY132 pKa = 10.52IYY134 pKa = 10.66LDD136 pKa = 4.38NYY138 pKa = 9.73YY139 pKa = 10.52QDD141 pKa = 3.67TDD143 pKa = 3.79TQVNICVQKK152 pKa = 10.33VQPTMYY158 pKa = 10.18EE159 pKa = 3.84LGSDD163 pKa = 4.13LVADD167 pKa = 4.69GVMQDD172 pKa = 4.5GEE174 pKa = 4.17VHH176 pKa = 5.81MYY178 pKa = 9.04EE179 pKa = 4.25FSVGEE184 pKa = 3.78EE185 pKa = 3.93ALYY188 pKa = 10.36EE189 pKa = 3.98IYY191 pKa = 10.87GCDD194 pKa = 5.34FILLDD199 pKa = 3.84EE200 pKa = 4.47NFNRR204 pKa = 11.84IEE206 pKa = 4.1CDD208 pKa = 3.69YY209 pKa = 10.35EE210 pKa = 4.93CSLSPDD216 pKa = 3.78EE217 pKa = 4.75IYY219 pKa = 11.13YY220 pKa = 10.21IAVADD225 pKa = 3.79KK226 pKa = 10.67GYY228 pKa = 10.39SAEE231 pKa = 4.23EE232 pKa = 3.73YY233 pKa = 8.42WYY235 pKa = 10.34DD236 pKa = 3.84VIVEE240 pKa = 4.74NVDD243 pKa = 2.67DD244 pKa = 4.97WYY246 pKa = 10.68DD247 pKa = 3.51DD248 pKa = 3.87DD249 pKa = 5.84YY250 pKa = 11.42EE251 pKa = 4.36YY252 pKa = 11.32TDD254 pKa = 5.22VEE256 pKa = 4.37PQGNDD261 pKa = 3.32YY262 pKa = 10.97IEE264 pKa = 4.42YY265 pKa = 10.59AVNEE269 pKa = 4.04YY270 pKa = 10.49DD271 pKa = 3.8GKK273 pKa = 9.7FTIGTRR279 pKa = 11.84EE280 pKa = 4.2GTSNPNDD287 pKa = 3.98DD288 pKa = 4.12NKK290 pKa = 11.35KK291 pKa = 8.95MLFGHH296 pKa = 7.5PYY298 pKa = 9.34PGTSFTTINLDD309 pKa = 3.33GEE311 pKa = 4.6YY312 pKa = 10.97AKK314 pKa = 9.28YY315 pKa = 9.06TPDD318 pKa = 3.65AEE320 pKa = 4.52SSISSGTTNMSEE332 pKa = 3.77QTMGDD337 pKa = 3.36VSVKK341 pKa = 10.07QVLSIVKK348 pKa = 10.16NSATQKK354 pKa = 10.36EE355 pKa = 4.51DD356 pKa = 3.22VVQIKK361 pKa = 10.46YY362 pKa = 10.26IVTNNDD368 pKa = 3.11GASHH372 pKa = 6.8DD373 pKa = 3.75VGVRR377 pKa = 11.84IMMDD381 pKa = 3.11TMLGDD386 pKa = 3.67NDD388 pKa = 4.0SAPFKK393 pKa = 10.8VPNVGNVLYY402 pKa = 10.32EE403 pKa = 4.03RR404 pKa = 11.84EE405 pKa = 4.13YY406 pKa = 10.5TGADD410 pKa = 3.29VPVYY414 pKa = 8.9WQAFDD419 pKa = 4.96SIEE422 pKa = 4.08NPTIVSQGTLRR433 pKa = 11.84DD434 pKa = 4.2GYY436 pKa = 9.21NTAPDD441 pKa = 3.76KK442 pKa = 11.4VQFASWGDD450 pKa = 3.36ITDD453 pKa = 3.88TLWDD457 pKa = 4.0YY458 pKa = 10.86EE459 pKa = 4.17VSEE462 pKa = 4.18YY463 pKa = 10.93QEE465 pKa = 4.63IGDD468 pKa = 4.22SSVAVYY474 pKa = 8.74WNPITIGPGARR485 pKa = 11.84KK486 pKa = 10.19DD487 pKa = 3.46FVTYY491 pKa = 10.49YY492 pKa = 10.89GLSEE496 pKa = 4.47FSANAASGMVLGVTAPSEE514 pKa = 3.94LVLDD518 pKa = 4.1KK519 pKa = 11.42EE520 pKa = 5.05EE521 pKa = 4.04YY522 pKa = 10.07TPDD525 pKa = 3.63PFIVTAYY532 pKa = 9.13IVNSGEE538 pKa = 3.9EE539 pKa = 4.0TSANTVVKK547 pKa = 10.55INLPEE552 pKa = 4.15GLNLYY557 pKa = 10.13SGSSEE562 pKa = 4.24VNVGDD567 pKa = 3.89VLPGEE572 pKa = 4.42EE573 pKa = 5.37KK574 pKa = 10.63LVSWYY579 pKa = 8.13VTAVKK584 pKa = 10.5RR585 pKa = 11.84EE586 pKa = 4.18TEE588 pKa = 3.87DD589 pKa = 3.11TLAYY593 pKa = 10.18SVEE596 pKa = 4.02LSSDD600 pKa = 3.22GVAGAFEE607 pKa = 4.39QKK609 pKa = 10.39HH610 pKa = 4.32ITVPSVSAIKK620 pKa = 10.14FADD623 pKa = 4.02PGLEE627 pKa = 3.85QAIRR631 pKa = 11.84DD632 pKa = 3.77ALGQSEE638 pKa = 4.33GAITKK643 pKa = 9.54TDD645 pKa = 3.83LEE647 pKa = 5.02SITALNAEE655 pKa = 4.3NLEE658 pKa = 3.99ISDD661 pKa = 4.1LSGIEE666 pKa = 3.78YY667 pKa = 10.44LVNLEE672 pKa = 4.0EE673 pKa = 6.02LKK675 pKa = 11.15LNYY678 pKa = 10.47NNIDD682 pKa = 3.85DD683 pKa = 4.97LNLVVDD689 pKa = 4.03NLQNLEE695 pKa = 3.97RR696 pKa = 11.84LEE698 pKa = 4.06LRR700 pKa = 11.84GNNIEE705 pKa = 4.52NIFISFFMQNSVVLAQEE722 pKa = 4.05RR723 pKa = 11.84LGATLGKK730 pKa = 10.19LPNLSYY736 pKa = 11.54LDD738 pKa = 3.89LSEE741 pKa = 5.38NNINNIEE748 pKa = 4.3GIEE751 pKa = 4.06NFTNIEE757 pKa = 4.12TLNLSANKK765 pKa = 9.65IDD767 pKa = 6.04DD768 pKa = 3.81IANLAGLSGMKK779 pKa = 9.98SLNISGNQVEE789 pKa = 5.66DD790 pKa = 3.32ITPLLEE796 pKa = 4.21VKK798 pKa = 9.47TLEE801 pKa = 4.64SVKK804 pKa = 10.85LQDD807 pKa = 3.7NPVVEE812 pKa = 4.51IPSEE816 pKa = 3.61ISEE819 pKa = 4.37IINVKK824 pKa = 10.47SLGKK828 pKa = 8.8KK829 pKa = 8.5TGITSNKK836 pKa = 5.68VWRR839 pKa = 11.84VQFSKK844 pKa = 11.13GLDD847 pKa = 3.09EE848 pKa = 4.04MLNYY852 pKa = 10.45NNYY855 pKa = 7.89VTVKK859 pKa = 10.39DD860 pKa = 3.66SLGNIVDD867 pKa = 3.57VDD869 pKa = 3.57VTYY872 pKa = 11.27SKK874 pKa = 11.3NEE876 pKa = 3.63KK877 pKa = 10.29ALLVTPKK884 pKa = 10.17SSYY887 pKa = 10.7SAGEE891 pKa = 4.13TYY893 pKa = 10.53SIVIGEE899 pKa = 4.89GILSEE904 pKa = 4.26KK905 pKa = 11.13GEE907 pKa = 4.37TLPEE911 pKa = 3.88AVRR914 pKa = 11.84MVFTVAQQ921 pKa = 3.65

Molecular weight: 101.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8TK11|W8TK11_PEPAC Pyruvate carboxylase OS=Peptoclostridium acidaminophilum DSM 3953 OX=1286171 GN=pyc PE=4 SV=1
MM1 pKa = 7.04IRR3 pKa = 11.84WYY5 pKa = 9.68ATGTSIAGRR14 pKa = 11.84RR15 pKa = 11.84FAIFILLTPSMLRR28 pKa = 11.84PIPNMSKK35 pKa = 10.82LPTTLISDD43 pKa = 4.08ITCCVSMPLRR53 pKa = 11.84NPAPRR58 pKa = 11.84VIAPWYY64 pKa = 9.63ISTEE68 pKa = 3.93MADD71 pKa = 4.32IITPMPIVDD80 pKa = 4.29ANIMADD86 pKa = 3.62TPSRR90 pKa = 11.84TDD92 pKa = 3.45FANSVLWSPLSPLSSAPTIAIAPTQYY118 pKa = 10.84ISVQLTKK125 pKa = 10.41PSATLPPLPLKK136 pKa = 10.58SSSRR140 pKa = 11.84RR141 pKa = 11.84SPNLISLWSISITSPITPPMTMEE164 pKa = 5.23NITSSRR170 pKa = 11.84LASSRR175 pKa = 11.84APLIPMRR182 pKa = 11.84TTHH185 pKa = 6.13SAIPFITVSLNFSVFIPLIIIPAKK209 pKa = 10.58LPATIAATLTSVPIIQSPPFF229 pKa = 3.97

Molecular weight: 24.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2843

0

2843

861762

35

2802

303.1

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.559 ± 0.057

1.18 ± 0.021

5.644 ± 0.044

7.775 ± 0.059

4.055 ± 0.034

7.336 ± 0.043

1.592 ± 0.016

8.262 ± 0.044

7.456 ± 0.042

8.854 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.021

4.271 ± 0.029

3.118 ± 0.024

2.708 ± 0.022

4.527 ± 0.035

6.778 ± 0.042

4.572 ± 0.028

6.903 ± 0.04

0.732 ± 0.015

3.584 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski