Saccharomonospora sp. LRS4.154

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharomo

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4456 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V9A086|A0A1V9A086_9PSEU MarR family transcriptional regulator OS=Saccharomonospora sp. LRS4.154 OX=1962155 GN=B1813_13515 PE=4 SV=1
MM1 pKa = 7.26TVRR4 pKa = 11.84DD5 pKa = 3.85TPYY8 pKa = 10.8PDD10 pKa = 4.5GTPCWVDD17 pKa = 4.25LMVADD22 pKa = 5.05PRR24 pKa = 11.84MAMDD28 pKa = 4.24FYY30 pKa = 11.59GALFGWNFADD40 pKa = 4.88QGDD43 pKa = 3.82EE44 pKa = 3.9AGNYY48 pKa = 8.08LLCSIDD54 pKa = 3.98DD55 pKa = 3.95FVVTGVGGMPPDD67 pKa = 3.74QPSPPVWTTYY77 pKa = 10.8LATSDD82 pKa = 4.0VDD84 pKa = 3.49DD85 pKa = 3.61TAARR89 pKa = 11.84VRR91 pKa = 11.84QAGGQLMVEE100 pKa = 4.47PMDD103 pKa = 3.69VMSEE107 pKa = 3.59GRR109 pKa = 11.84MAMAADD115 pKa = 3.45PTGAVFGLWQAGRR128 pKa = 11.84TIGAQLTGTPSALVWNEE145 pKa = 3.09CMTRR149 pKa = 11.84DD150 pKa = 3.33FATAKK155 pKa = 10.44GFYY158 pKa = 9.64TDD160 pKa = 3.19VFGYY164 pKa = 10.38AAEE167 pKa = 4.65DD168 pKa = 3.46MSDD171 pKa = 3.43EE172 pKa = 4.17QFTYY176 pKa = 10.93AALLVGDD183 pKa = 4.81RR184 pKa = 11.84YY185 pKa = 10.89VGGVGEE191 pKa = 4.96LPADD195 pKa = 3.9VPADD199 pKa = 3.93VPPHH203 pKa = 5.25WMTYY207 pKa = 10.41FGVADD212 pKa = 3.74TDD214 pKa = 3.83AAVARR219 pKa = 11.84VGDD222 pKa = 3.86LGGSVVTAPFDD233 pKa = 3.68TPYY236 pKa = 11.4GRR238 pKa = 11.84MAAVADD244 pKa = 3.9NQGVAFSVIAVTAVPDD260 pKa = 3.69SPEE263 pKa = 3.49

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V9A125|A0A1V9A125_9PSEU Non-specific serine/threonine protein kinase OS=Saccharomonospora sp. LRS4.154 OX=1962155 GN=B1813_14430 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.89GRR42 pKa = 11.84ARR44 pKa = 11.84LSAA47 pKa = 3.91

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4456

0

4456

1458872

33

5196

327.4

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.464 ± 0.056

0.753 ± 0.01

6.41 ± 0.032

5.947 ± 0.035

2.686 ± 0.024

9.406 ± 0.036

2.289 ± 0.016

2.865 ± 0.024

1.55 ± 0.026

10.499 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.595 ± 0.014

1.637 ± 0.016

5.853 ± 0.029

2.576 ± 0.024

8.386 ± 0.044

5.181 ± 0.026

6.103 ± 0.026

9.391 ± 0.033

1.489 ± 0.014

1.921 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski