Eel River basin pequenovirus

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5AK08|A0A0C5AK08_9VIRU Putative replication initiation protein OS=Eel River basin pequenovirus OX=1609634 PE=4 SV=1
MM1 pKa = 7.59LVPEE5 pKa = 4.25VQGNQLYY12 pKa = 10.77YY13 pKa = 10.56DD14 pKa = 3.9ISSKK18 pKa = 9.62TEE20 pKa = 3.75FSTSEE25 pKa = 3.39IAAIDD30 pKa = 3.88SNISGDD36 pKa = 3.45EE37 pKa = 4.14YY38 pKa = 11.58YY39 pKa = 10.19PYY41 pKa = 11.41AWTSTVNDD49 pKa = 3.19EE50 pKa = 4.23LANYY54 pKa = 9.69RR55 pKa = 11.84YY56 pKa = 10.18YY57 pKa = 11.33VDD59 pKa = 3.84NSVDD63 pKa = 3.61LVAGSRR69 pKa = 11.84VAFGIFLAPDD79 pKa = 3.45VNKK82 pKa = 11.07DD83 pKa = 3.14NMLINIEE90 pKa = 3.99GAMAFYY96 pKa = 10.93GDD98 pKa = 3.54STRR101 pKa = 11.84FNVYY105 pKa = 9.77PILGTTVSSTITSSKK120 pKa = 10.2AAQSNQLACWKK131 pKa = 9.71VIPCEE136 pKa = 4.19TQDD139 pKa = 3.28GAGYY143 pKa = 10.27SKK145 pKa = 10.76LVSFNKK151 pKa = 9.22TVLAVEE157 pKa = 4.09QSSVNTYY164 pKa = 10.02CLAWVISNHH173 pKa = 5.71EE174 pKa = 3.95GSTRR178 pKa = 11.84SLEE181 pKa = 4.31FNSNLSMRR189 pKa = 11.84AWTTPINYY197 pKa = 8.24HH198 pKa = 6.43RR199 pKa = 11.84PGAA202 pKa = 3.85

Molecular weight: 22.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5AK13|A0A0C5AK13_9VIRU Uncharacterized protein OS=Eel River basin pequenovirus OX=1609634 PE=4 SV=1
MM1 pKa = 7.53LSIIKK6 pKa = 9.28NQKK9 pKa = 8.98LKK11 pKa = 11.05KK12 pKa = 10.44LIIRR16 pKa = 11.84YY17 pKa = 9.47KK18 pKa = 9.5MRR20 pKa = 11.84SLRR23 pKa = 11.84LSISKK28 pKa = 10.02SLKK31 pKa = 9.13NLKK34 pKa = 9.92EE35 pKa = 3.95NSLKK39 pKa = 10.54ARR41 pKa = 11.84KK42 pKa = 9.66AEE44 pKa = 3.85LLTRR48 pKa = 11.84LEE50 pKa = 4.16YY51 pKa = 10.54EE52 pKa = 3.6LEE54 pKa = 3.99YY55 pKa = 10.46RR56 pKa = 11.84IKK58 pKa = 10.93NGWYY62 pKa = 10.31CIFNTLTVNEE72 pKa = 4.17SSIEE76 pKa = 4.0KK77 pKa = 10.1VFEE80 pKa = 4.12KK81 pKa = 10.62GSRR84 pKa = 11.84IFSDD88 pKa = 3.72YY89 pKa = 10.69VRR91 pKa = 11.84SLDD94 pKa = 3.56RR95 pKa = 11.84GVGIAIHH102 pKa = 6.12KK103 pKa = 9.13NWRR106 pKa = 11.84QAVTKK111 pKa = 9.95RR112 pKa = 11.84KK113 pKa = 9.46EE114 pKa = 3.96GNEE117 pKa = 3.51FHH119 pKa = 6.96TYY121 pKa = 9.02FAVVEE126 pKa = 4.24RR127 pKa = 11.84GTKK130 pKa = 9.63NGRR133 pKa = 11.84LHH135 pKa = 6.1IHH137 pKa = 6.84VIHH140 pKa = 6.3MMKK143 pKa = 10.12EE144 pKa = 4.08LPNGCVDD151 pKa = 4.06PNAGRR156 pKa = 11.84AIPNRR161 pKa = 11.84RR162 pKa = 11.84EE163 pKa = 3.44VTYY166 pKa = 10.56LKK168 pKa = 10.42RR169 pKa = 11.84YY170 pKa = 6.75WKK172 pKa = 10.01YY173 pKa = 10.93GYY175 pKa = 9.36SAPIAVRR182 pKa = 11.84FNTNDD187 pKa = 3.29AFGKK191 pKa = 10.08KK192 pKa = 6.95YY193 pKa = 9.55WRR195 pKa = 11.84FPVKK199 pKa = 10.06EE200 pKa = 3.83VAKK203 pKa = 10.83NRR205 pKa = 11.84FEE207 pKa = 4.65SLEE210 pKa = 4.22CKK212 pKa = 10.28DD213 pKa = 3.49AGSIIGYY220 pKa = 8.39IGKK223 pKa = 10.04YY224 pKa = 5.93MTKK227 pKa = 10.05EE228 pKa = 3.48YY229 pKa = 10.75DD230 pKa = 3.26KK231 pKa = 10.45PLEE234 pKa = 4.18KK235 pKa = 10.35EE236 pKa = 4.05YY237 pKa = 10.0VTWRR241 pKa = 11.84TRR243 pKa = 11.84LTRR246 pKa = 11.84TLGTQIMKK254 pKa = 9.99SLCRR258 pKa = 11.84VVTKK262 pKa = 10.85NQLLLLMTMKK272 pKa = 9.38STKK275 pKa = 8.21EE276 pKa = 3.68MKK278 pKa = 10.43VLEE281 pKa = 4.76KK282 pKa = 10.39PLPLYY287 pKa = 10.45KK288 pKa = 10.43LRR290 pKa = 11.84TIATQEE296 pKa = 3.65YY297 pKa = 8.27MRR299 pKa = 11.84RR300 pKa = 11.84SLTNQPLKK308 pKa = 10.16IWEE311 pKa = 4.32SLICLTPRR319 pKa = 11.84MSLLTHH325 pKa = 6.05VKK327 pKa = 10.84SMIQEE332 pKa = 3.49KK333 pKa = 7.33WTYY336 pKa = 10.3RR337 pKa = 11.84LQNYY341 pKa = 8.36GITMTQIMSNMEE353 pKa = 3.68GSNINKK359 pKa = 9.45ILNNLEE365 pKa = 3.63KK366 pKa = 10.51QIYY369 pKa = 7.87GKK371 pKa = 10.6KK372 pKa = 8.64EE373 pKa = 3.2IKK375 pKa = 10.15YY376 pKa = 7.95YY377 pKa = 10.91ARR379 pKa = 11.84GVSMEE384 pKa = 4.21TLCC387 pKa = 5.64

Molecular weight: 45.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1824

59

428

202.7

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.798 ± 1.455

0.932 ± 0.22

5.099 ± 1.031

6.086 ± 0.704

3.618 ± 0.458

5.428 ± 0.688

1.7 ± 0.368

7.292 ± 0.514

8.991 ± 1.694

8.717 ± 0.752

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.481

6.14 ± 0.475

2.961 ± 0.428

3.673 ± 0.626

4.825 ± 0.75

7.182 ± 0.711

6.579 ± 0.639

5.482 ± 0.656

1.371 ± 0.31

4.715 ± 0.743
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski