Amino acid dipepetide frequency for Brochothrix thermosphacta (Microbacterium thermosphactum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.402AlaAla: 6.402 ± 0.161	0.513AlaCys: 0.513 ± 0.026	4.315AlaAsp: 4.315 ± 0.08	5.116AlaGlu: 5.116 ± 0.113	3.241AlaPhe: 3.241 ± 0.07	5.314AlaGly: 5.314 ± 0.102	1.385AlaHis: 1.385 ± 0.051	6.693AlaIle: 6.693 ± 0.115	4.995AlaLys: 4.995 ± 0.098	7.973AlaLeu: 7.973 ± 0.134	2.172AlaMet: 2.172 ± 0.064	3.363AlaAsn: 3.363 ± 0.072	2.245AlaPro: 2.245 ± 0.088	2.711AlaGln: 2.711 ± 0.062	2.66AlaArg: 2.66 ± 0.063	4.198AlaSer: 4.198 ± 0.094	4.776AlaThr: 4.776 ± 0.085	5.845AlaVal: 5.845 ± 0.101	0.532AlaTrp: 0.532 ± 0.027	2.602AlaTyr: 2.602 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.406CysAla: 0.406 ± 0.025	0.077CysCys: 0.077 ± 0.011	0.365CysAsp: 0.365 ± 0.025	0.434CysGlu: 0.434 ± 0.028	0.303CysPhe: 0.303 ± 0.02	0.584CysGly: 0.584 ± 0.028	0.201CysHis: 0.201 ± 0.015	0.458CysIle: 0.458 ± 0.028	0.252CysLys: 0.252 ± 0.02	0.673CysLeu: 0.673 ± 0.037	0.136CysMet: 0.136 ± 0.016	0.223CysAsn: 0.223 ± 0.018	0.215CysPro: 0.215 ± 0.018	0.211CysGln: 0.211 ± 0.018	0.219CysArg: 0.219 ± 0.016	0.412CysSer: 0.412 ± 0.024	0.289CysThr: 0.289 ± 0.022	0.445CysVal: 0.445 ± 0.023	0.062CysTrp: 0.062 ± 0.01	0.251CysTyr: 0.251 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	3.685AspAla: 3.685 ± 0.077	0.308AspCys: 0.308 ± 0.024	2.696AspAsp: 2.696 ± 0.069	4.302AspGlu: 4.302 ± 0.094	2.559AspPhe: 2.559 ± 0.061	3.27AspGly: 3.27 ± 0.078	0.833AspHis: 0.833 ± 0.034	4.214AspIle: 4.214 ± 0.086	3.849AspLys: 3.849 ± 0.097	4.581AspLeu: 4.581 ± 0.092	1.395AspMet: 1.395 ± 0.044	2.448AspAsn: 2.448 ± 0.061	1.513AspPro: 1.513 ± 0.046	1.498AspGln: 1.498 ± 0.044	1.772AspArg: 1.772 ± 0.053	3.046AspSer: 3.046 ± 0.091	3.134AspThr: 3.134 ± 0.072	4.35AspVal: 4.35 ± 0.084	0.602AspTrp: 0.602 ± 0.029	2.402AspTyr: 2.402 ± 0.061	0.0AspXaa: 0.0 ± 0.0
Glu	5.784GluAla: 5.784 ± 0.113	0.348GluCys: 0.348 ± 0.026	3.201GluAsp: 3.201 ± 0.074	4.991GluGlu: 4.991 ± 0.11	2.201GluPhe: 2.201 ± 0.064	3.854GluGly: 3.854 ± 0.078	1.322GluHis: 1.322 ± 0.042	5.054GluIle: 5.054 ± 0.099	6.063GluLys: 6.063 ± 0.11	6.696GluLeu: 6.696 ± 0.112	2.093GluMet: 2.093 ± 0.063	3.616GluAsn: 3.616 ± 0.082	1.669GluPro: 1.669 ± 0.056	3.081GluGln: 3.081 ± 0.096	3.119GluArg: 3.119 ± 0.081	3.382GluSer: 3.382 ± 0.073	4.465GluThr: 4.465 ± 0.09	4.705GluVal: 4.705 ± 0.099	0.706GluTrp: 0.706 ± 0.036	1.888GluTyr: 1.888 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.959PheAla: 2.959 ± 0.082	0.323PheCys: 0.323 ± 0.021	2.487PheAsp: 2.487 ± 0.059	2.919PheGlu: 2.919 ± 0.073	2.208PhePhe: 2.208 ± 0.068	3.068PheGly: 3.068 ± 0.07	0.676PheHis: 0.676 ± 0.031	3.967PheIle: 3.967 ± 0.087	3.001PheLys: 3.001 ± 0.074	4.122PheLeu: 4.122 ± 0.101	1.114PheMet: 1.114 ± 0.042	2.226PheAsn: 2.226 ± 0.052	1.417PhePro: 1.417 ± 0.04	1.274PheGln: 1.274 ± 0.047	1.176PheArg: 1.176 ± 0.045	3.141PheSer: 3.141 ± 0.08	2.601PheThr: 2.601 ± 0.065	3.181PheVal: 3.181 ± 0.082	0.406PheTrp: 0.406 ± 0.027	1.775PheTyr: 1.775 ± 0.057	0.0PheXaa: 0.0 ± 0.0
Gly	4.746GlyAla: 4.746 ± 0.096	0.551GlyCys: 0.551 ± 0.028	3.222GlyAsp: 3.222 ± 0.077	4.063GlyGlu: 4.063 ± 0.078	3.266GlyPhe: 3.266 ± 0.075	4.387GlyGly: 4.387 ± 0.092	1.309GlyHis: 1.309 ± 0.047	5.583GlyIle: 5.583 ± 0.103	4.488GlyLys: 4.488 ± 0.093	6.348GlyLeu: 6.348 ± 0.126	1.768GlyMet: 1.768 ± 0.06	2.628GlyAsn: 2.628 ± 0.072	1.49GlyPro: 1.49 ± 0.047	2.13GlyGln: 2.13 ± 0.058	2.383GlyArg: 2.383 ± 0.071	3.775GlySer: 3.775 ± 0.077	4.019GlyThr: 4.019 ± 0.083	4.906GlyVal: 4.906 ± 0.096	0.689GlyTrp: 0.689 ± 0.032	2.702GlyTyr: 2.702 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.163HisAla: 1.163 ± 0.037	0.167HisCys: 0.167 ± 0.014	0.996HisAsp: 0.996 ± 0.04	1.157HisGlu: 1.157 ± 0.045	0.983HisPhe: 0.983 ± 0.041	1.187HisGly: 1.187 ± 0.044	0.552HisHis: 0.552 ± 0.026	1.344HisIle: 1.344 ± 0.04	0.97HisLys: 0.97 ± 0.037	1.988HisLeu: 1.988 ± 0.061	0.386HisMet: 0.386 ± 0.022	0.778HisAsn: 0.778 ± 0.032	0.922HisPro: 0.922 ± 0.037	0.898HisGln: 0.898 ± 0.035	0.855HisArg: 0.855 ± 0.036	1.114HisSer: 1.114 ± 0.046	1.081HisThr: 1.081 ± 0.038	1.299HisVal: 1.299 ± 0.047	0.195HisTrp: 0.195 ± 0.016	0.859HisTyr: 0.859 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	7.01IleAla: 7.01 ± 0.129	0.614IleCys: 0.614 ± 0.028	4.592IleAsp: 4.592 ± 0.078	5.657IleGlu: 5.657 ± 0.095	3.087IlePhe: 3.087 ± 0.083	5.702IleGly: 5.702 ± 0.102	1.373IleHis: 1.373 ± 0.046	6.367IleIle: 6.367 ± 0.138	5.113IleLys: 5.113 ± 0.09	6.755IleLeu: 6.755 ± 0.12	1.838IleMet: 1.838 ± 0.061	3.647IleAsn: 3.647 ± 0.08	2.923IlePro: 2.923 ± 0.065	2.593IleGln: 2.593 ± 0.064	2.694IleArg: 2.694 ± 0.057	5.186IleSer: 5.186 ± 0.089	5.251IleThr: 5.251 ± 0.094	5.904IleVal: 5.904 ± 0.101	0.615IleTrp: 0.615 ± 0.032	2.365IleTyr: 2.365 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.155LysAla: 5.155 ± 0.117	0.243LysCys: 0.243 ± 0.02	3.749LysAsp: 3.749 ± 0.093	5.782LysGlu: 5.782 ± 0.108	1.902LysPhe: 1.902 ± 0.052	4.007LysGly: 4.007 ± 0.086	1.237LysHis: 1.237 ± 0.039	5.08LysIle: 5.08 ± 0.09	6.127LysLys: 6.127 ± 0.127	5.83LysLeu: 5.83 ± 0.092	2.45LysMet: 2.45 ± 0.06	3.789LysAsn: 3.789 ± 0.084	2.036LysPro: 2.036 ± 0.057	3.283LysGln: 3.283 ± 0.08	3.251LysArg: 3.251 ± 0.07	3.42LysSer: 3.42 ± 0.083	4.484LysThr: 4.484 ± 0.097	4.713LysVal: 4.713 ± 0.115	0.652LysTrp: 0.652 ± 0.031	2.402LysTyr: 2.402 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	8.233LeuAla: 8.233 ± 0.134	0.548LeuCys: 0.548 ± 0.024	4.802LeuAsp: 4.802 ± 0.085	6.105LeuGlu: 6.105 ± 0.114	4.591LeuPhe: 4.591 ± 0.11	5.865LeuGly: 5.865 ± 0.112	1.668LeuHis: 1.668 ± 0.047	7.585LeuIle: 7.585 ± 0.14	6.577LeuLys: 6.577 ± 0.11	10.108LeuLeu: 10.108 ± 0.194	2.633LeuMet: 2.633 ± 0.063	4.72LeuAsn: 4.72 ± 0.079	3.93LeuPro: 3.93 ± 0.078	3.296LeuGln: 3.296 ± 0.088	3.307LeuArg: 3.307 ± 0.071	6.845LeuSer: 6.845 ± 0.101	6.604LeuThr: 6.604 ± 0.127	6.463LeuVal: 6.463 ± 0.121	0.64LeuTrp: 0.64 ± 0.029	2.933LeuTyr: 2.933 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	2.095MetAla: 2.095 ± 0.061	0.147MetCys: 0.147 ± 0.015	1.242MetAsp: 1.242 ± 0.041	1.311MetGlu: 1.311 ± 0.048	1.017MetPhe: 1.017 ± 0.037	1.735MetGly: 1.735 ± 0.045	0.454MetHis: 0.454 ± 0.022	2.15MetIle: 2.15 ± 0.058	2.247MetLys: 2.247 ± 0.06	2.557MetLeu: 2.557 ± 0.061	0.884MetMet: 0.884 ± 0.045	1.413MetAsn: 1.413 ± 0.053	1.065MetPro: 1.065 ± 0.036	0.865MetGln: 0.865 ± 0.036	1.052MetArg: 1.052 ± 0.036	1.91MetSer: 1.91 ± 0.047	2.049MetThr: 2.049 ± 0.053	1.731MetVal: 1.731 ± 0.054	0.166MetTrp: 0.166 ± 0.015	0.711MetTyr: 0.711 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.201AsnAla: 3.201 ± 0.072	0.307AsnCys: 0.307 ± 0.023	2.661AsnAsp: 2.661 ± 0.065	3.326AsnGlu: 3.326 ± 0.083	1.891AsnPhe: 1.891 ± 0.057	3.272AsnGly: 3.272 ± 0.089	0.951AsnHis: 0.951 ± 0.034	3.697AsnIle: 3.697 ± 0.08	3.595AsnLys: 3.595 ± 0.091	3.782AsnLeu: 3.782 ± 0.075	1.203AsnMet: 1.203 ± 0.04	2.548AsnAsn: 2.548 ± 0.073	1.914AsnPro: 1.914 ± 0.051	1.854AsnGln: 1.854 ± 0.056	1.905AsnArg: 1.905 ± 0.058	2.491AsnSer: 2.491 ± 0.062	2.705AsnThr: 2.705 ± 0.073	3.231AsnVal: 3.231 ± 0.068	0.478AsnTrp: 0.478 ± 0.031	1.973AsnTyr: 1.973 ± 0.064	0.0AsnXaa: 0.0 ± 0.0
Pro	2.394ProAla: 2.394 ± 0.062	0.136ProCys: 0.136 ± 0.015	1.84ProAsp: 1.84 ± 0.077	2.643ProGlu: 2.643 ± 0.071	1.805ProPhe: 1.805 ± 0.054	1.935ProGly: 1.935 ± 0.07	0.714ProHis: 0.714 ± 0.031	2.631ProIle: 2.631 ± 0.061	1.995ProLys: 1.995 ± 0.051	3.486ProLeu: 3.486 ± 0.075	0.78ProMet: 0.78 ± 0.033	1.488ProAsn: 1.488 ± 0.048	0.682ProPro: 0.682 ± 0.037	1.22ProGln: 1.22 ± 0.045	0.92ProArg: 0.92 ± 0.036	1.998ProSer: 1.998 ± 0.084	2.408ProThr: 2.408 ± 0.065	2.643ProVal: 2.643 ± 0.078	0.247ProTrp: 0.247 ± 0.017	1.291ProTyr: 1.291 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.027GlnAla: 3.027 ± 0.077	0.156GlnCys: 0.156 ± 0.015	1.344GlnAsp: 1.344 ± 0.05	2.254GlnGlu: 2.254 ± 0.064	1.527GlnPhe: 1.527 ± 0.047	2.009GlnGly: 2.009 ± 0.056	0.806GlnHis: 0.806 ± 0.033	2.53GlnIle: 2.53 ± 0.063	2.648GlnLys: 2.648 ± 0.064	4.55GlnLeu: 4.55 ± 0.096	1.002GlnMet: 1.002 ± 0.041	1.507GlnAsn: 1.507 ± 0.044	1.361GlnPro: 1.361 ± 0.056	2.006GlnGln: 2.006 ± 0.073	1.782GlnArg: 1.782 ± 0.056	2.138GlnSer: 2.138 ± 0.058	2.604GlnThr: 2.604 ± 0.068	2.328GlnVal: 2.328 ± 0.063	0.388GlnTrp: 0.388 ± 0.028	1.092GlnTyr: 1.092 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.423ArgAla: 2.423 ± 0.06	0.248ArgCys: 0.248 ± 0.019	1.821ArgAsp: 1.821 ± 0.051	2.713ArgGlu: 2.713 ± 0.074	1.925ArgPhe: 1.925 ± 0.058	2.12ArgGly: 2.12 ± 0.057	0.883ArgHis: 0.883 ± 0.037	2.963ArgIle: 2.963 ± 0.067	2.471ArgLys: 2.471 ± 0.06	4.085ArgLeu: 4.085 ± 0.081	1.113ArgMet: 1.113 ± 0.039	1.568ArgAsn: 1.568 ± 0.051	1.21ArgPro: 1.21 ± 0.041	1.638ArgGln: 1.638 ± 0.048	1.777ArgArg: 1.777 ± 0.052	1.972ArgSer: 1.972 ± 0.049	1.958ArgThr: 1.958 ± 0.053	2.678ArgVal: 2.678 ± 0.069	0.354ArgTrp: 0.354 ± 0.022	1.701ArgTyr: 1.701 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.298SerAla: 4.298 ± 0.089	0.326SerCys: 0.326 ± 0.022	3.289SerAsp: 3.289 ± 0.077	4.044SerGlu: 4.044 ± 0.096	3.114SerPhe: 3.114 ± 0.074	4.329SerGly: 4.329 ± 0.092	1.165SerHis: 1.165 ± 0.039	4.637SerIle: 4.637 ± 0.093	3.878SerLys: 3.878 ± 0.081	5.849SerLeu: 5.849 ± 0.095	1.523SerMet: 1.523 ± 0.054	2.815SerAsn: 2.815 ± 0.078	1.872SerPro: 1.872 ± 0.058	2.165SerGln: 2.165 ± 0.053	2.253SerArg: 2.253 ± 0.064	3.723SerSer: 3.723 ± 0.114	3.51SerThr: 3.51 ± 0.105	4.235SerVal: 4.235 ± 0.081	0.573SerTrp: 0.573 ± 0.03	2.424SerTyr: 2.424 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	5.242ThrAla: 5.242 ± 0.102	0.293ThrCys: 0.293 ± 0.018	3.479ThrAsp: 3.479 ± 0.089	4.018ThrGlu: 4.018 ± 0.084	2.802ThrPhe: 2.802 ± 0.075	4.289ThrGly: 4.289 ± 0.092	1.233ThrHis: 1.233 ± 0.044	5.264ThrIle: 5.264 ± 0.099	3.94ThrLys: 3.94 ± 0.088	6.387ThrLeu: 6.387 ± 0.101	1.464ThrMet: 1.464 ± 0.042	2.893ThrAsn: 2.893 ± 0.062	2.611ThrPro: 2.611 ± 0.083	2.038ThrGln: 2.038 ± 0.051	1.997ThrArg: 1.997 ± 0.06	3.655ThrSer: 3.655 ± 0.08	4.487ThrThr: 4.487 ± 0.109	5.123ThrVal: 5.123 ± 0.106	0.448ThrTrp: 0.448 ± 0.029	2.295ThrTyr: 2.295 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	5.945ValAla: 5.945 ± 0.12	0.523ValCys: 0.523 ± 0.025	3.785ValAsp: 3.785 ± 0.083	4.536ValGlu: 4.536 ± 0.089	3.289ValPhe: 3.289 ± 0.073	4.646ValGly: 4.646 ± 0.093	1.08ValHis: 1.08 ± 0.042	6.002ValIle: 6.002 ± 0.103	4.825ValLys: 4.825 ± 0.099	6.993ValLeu: 6.993 ± 0.107	1.909ValMet: 1.909 ± 0.048	3.235ValAsn: 3.235 ± 0.08	2.663ValPro: 2.663 ± 0.064	2.086ValGln: 2.086 ± 0.056	2.384ValArg: 2.384 ± 0.059	4.832ValSer: 4.832 ± 0.094	4.959ValThr: 4.959 ± 0.088	5.686ValVal: 5.686 ± 0.113	0.562ValTrp: 0.562 ± 0.03	2.236ValTyr: 2.236 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.508TrpAla: 0.508 ± 0.026	0.078TrpCys: 0.078 ± 0.011	0.51TrpAsp: 0.51 ± 0.028	0.563TrpGlu: 0.563 ± 0.029	0.412TrpPhe: 0.412 ± 0.026	0.581TrpGly: 0.581 ± 0.028	0.222TrpHis: 0.222 ± 0.018	0.607TrpIle: 0.607 ± 0.032	0.551TrpLys: 0.551 ± 0.031	1.102TrpLeu: 1.102 ± 0.042	0.217TrpMet: 0.217 ± 0.018	0.384TrpAsn: 0.384 ± 0.025	0.21TrpPro: 0.21 ± 0.017	0.451TrpGln: 0.451 ± 0.028	0.385TrpArg: 0.385 ± 0.021	0.551TrpSer: 0.551 ± 0.035	0.478TrpThr: 0.478 ± 0.031	0.515TrpVal: 0.515 ± 0.03	0.112TrpTrp: 0.112 ± 0.013	0.329TrpTyr: 0.329 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.374TyrAla: 2.374 ± 0.056	0.31TyrCys: 0.31 ± 0.021	2.058TyrAsp: 2.058 ± 0.053	2.304TyrGlu: 2.304 ± 0.061	1.919TyrPhe: 1.919 ± 0.052	2.298TyrGly: 2.298 ± 0.058	0.832TyrHis: 0.832 ± 0.031	2.458TyrIle: 2.458 ± 0.059	2.009TyrLys: 2.009 ± 0.057	3.586TyrLeu: 3.586 ± 0.09	0.722TyrMet: 0.722 ± 0.03	1.699TyrAsn: 1.699 ± 0.049	1.309TyrPro: 1.309 ± 0.044	1.762TyrGln: 1.762 ± 0.055	1.729TyrArg: 1.729 ± 0.054	2.23TyrSer: 2.23 ± 0.052	2.095TyrThr: 2.095 ± 0.056	2.198TyrVal: 2.198 ± 0.049	0.341TyrTrp: 0.341 ± 0.024	1.442TyrTyr: 1.442 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2406 proteins (729719 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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