Streptococcus phage Javan486

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BBD0|A0A4D6BBD0_9CAUD YopX domain-containing protein OS=Streptococcus phage Javan486 OX=2548201 GN=Javan486_0037 PE=4 SV=1
MM1 pKa = 7.88TIRR4 pKa = 11.84EE5 pKa = 4.24MLISNQILNNDD16 pKa = 5.05DD17 pKa = 3.53IDD19 pKa = 4.57FMLSVNQDD27 pKa = 3.1VSPLFEE33 pKa = 6.68LEE35 pKa = 5.16DD36 pKa = 4.53NDD38 pKa = 5.57DD39 pKa = 3.73NWDD42 pKa = 3.67VLVDD46 pKa = 3.79EE47 pKa = 5.17LQQTSSYY54 pKa = 9.47LTGDD58 pKa = 3.74GEE60 pKa = 4.84SVSDD64 pKa = 3.5RR65 pKa = 11.84TKK67 pKa = 11.12KK68 pKa = 10.67FDD70 pKa = 4.13DD71 pKa = 4.21LVDD74 pKa = 4.67KK75 pKa = 11.11ISAII79 pKa = 3.85

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B9A4|A0A4D6B9A4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan486 OX=2548201 GN=Javan486_0031 PE=4 SV=1
MM1 pKa = 7.38AKK3 pKa = 10.08VVVEE7 pKa = 3.73LGGIKK12 pKa = 10.21RR13 pKa = 11.84KK14 pKa = 10.05VSPQALAKK22 pKa = 10.53GKK24 pKa = 10.57LIMNNQVMMSMNPYY38 pKa = 9.39VPYY41 pKa = 10.15RR42 pKa = 11.84DD43 pKa = 3.27GALRR47 pKa = 11.84GSSRR51 pKa = 11.84ANSVGVTWSGPHH63 pKa = 6.37ARR65 pKa = 11.84AQFYY69 pKa = 10.53GGAYY73 pKa = 9.93NKK75 pKa = 10.37YY76 pKa = 10.46KK77 pKa = 10.58SFKK80 pKa = 8.64FKK82 pKa = 10.8KK83 pKa = 8.45YY84 pKa = 5.33TTPGTGKK91 pKa = 10.19RR92 pKa = 11.84WDD94 pKa = 3.49KK95 pKa = 10.88RR96 pKa = 11.84ALANATIVKK105 pKa = 9.88DD106 pKa = 3.5WEE108 pKa = 4.16KK109 pKa = 11.26SLLRR113 pKa = 11.84GMGFKK118 pKa = 10.45

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12034

39

1086

200.6

22.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.155 ± 0.605

0.64 ± 0.099

6.714 ± 0.309

7.263 ± 0.37

3.69 ± 0.217

6.291 ± 0.256

1.579 ± 0.158

6.781 ± 0.25

8.667 ± 0.29

8.767 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.142

5.592 ± 0.207

2.684 ± 0.23

3.922 ± 0.229

4.363 ± 0.254

6.191 ± 0.372

6.191 ± 0.296

6.257 ± 0.274

1.097 ± 0.101

3.781 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski