Amino acid dipepetide frequency for Mycobacterium phage Kazan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.32AlaAla: 11.32 ± 1.079
0.554AlaCys: 0.554 ± 0.169
5.414AlaAsp: 5.414 ± 0.565
7.444AlaGlu: 7.444 ± 0.812
3.138AlaPhe: 3.138 ± 0.462
8.244AlaGly: 8.244 ± 0.841
1.6AlaHis: 1.6 ± 0.36
3.63AlaIle: 3.63 ± 0.443
5.106AlaLys: 5.106 ± 0.609
6.952AlaLeu: 6.952 ± 0.665
2.461AlaMet: 2.461 ± 0.345
3.015AlaAsn: 3.015 ± 0.499
4.553AlaPro: 4.553 ± 0.838
3.753AlaGln: 3.753 ± 0.662
6.398AlaArg: 6.398 ± 0.713
4.614AlaSer: 4.614 ± 0.584
4.799AlaThr: 4.799 ± 0.584
6.398AlaVal: 6.398 ± 0.689
1.6AlaTrp: 1.6 ± 0.312
2.461AlaTyr: 2.461 ± 0.5
0.0AlaXaa: 0.0 ± 0.0
Cys
0.308CysAla: 0.308 ± 0.119
0.062CysCys: 0.062 ± 0.058
0.677CysAsp: 0.677 ± 0.217
0.8CysGlu: 0.8 ± 0.247
0.369CysPhe: 0.369 ± 0.121
0.615CysGly: 0.615 ± 0.177
0.246CysHis: 0.246 ± 0.119
0.369CysIle: 0.369 ± 0.181
0.308CysLys: 0.308 ± 0.131
0.861CysLeu: 0.861 ± 0.251
0.062CysMet: 0.062 ± 0.056
0.677CysAsn: 0.677 ± 0.205
0.923CysPro: 0.923 ± 0.274
0.369CysGln: 0.369 ± 0.156
0.615CysArg: 0.615 ± 0.243
0.431CysSer: 0.431 ± 0.173
0.554CysThr: 0.554 ± 0.173
0.8CysVal: 0.8 ± 0.216
0.369CysTrp: 0.369 ± 0.124
0.185CysTyr: 0.185 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
6.398AspAla: 6.398 ± 0.647
0.677AspCys: 0.677 ± 0.211
3.814AspAsp: 3.814 ± 0.459
3.753AspGlu: 3.753 ± 0.503
2.707AspPhe: 2.707 ± 0.46
6.398AspGly: 6.398 ± 0.577
1.477AspHis: 1.477 ± 0.32
3.322AspIle: 3.322 ± 0.629
2.707AspLys: 2.707 ± 0.402
5.906AspLeu: 5.906 ± 0.903
1.354AspMet: 1.354 ± 0.314
1.969AspAsn: 1.969 ± 0.351
4.491AspPro: 4.491 ± 0.578
2.03AspGln: 2.03 ± 0.383
3.199AspArg: 3.199 ± 0.498
1.969AspSer: 1.969 ± 0.332
3.876AspThr: 3.876 ± 0.427
4.676AspVal: 4.676 ± 0.584
1.723AspTrp: 1.723 ± 0.324
2.215AspTyr: 2.215 ± 0.419
0.0AspXaa: 0.0 ± 0.0
Glu
6.583GluAla: 6.583 ± 0.812
0.369GluCys: 0.369 ± 0.148
5.045GluAsp: 5.045 ± 0.585
4.737GluGlu: 4.737 ± 0.663
3.261GluPhe: 3.261 ± 0.408
5.291GluGly: 5.291 ± 0.586
1.477GluHis: 1.477 ± 0.349
4.061GluIle: 4.061 ± 0.412
2.584GluLys: 2.584 ± 0.411
6.521GluLeu: 6.521 ± 0.775
1.907GluMet: 1.907 ± 0.364
2.707GluAsn: 2.707 ± 0.328
2.707GluPro: 2.707 ± 0.476
3.015GluGln: 3.015 ± 0.426
4.061GluArg: 4.061 ± 0.551
3.753GluSer: 3.753 ± 0.419
3.076GluThr: 3.076 ± 0.473
4.245GluVal: 4.245 ± 0.45
1.169GluTrp: 1.169 ± 0.321
1.846GluTyr: 1.846 ± 0.351
0.0GluXaa: 0.0 ± 0.0
Phe
2.953PheAla: 2.953 ± 0.387
0.431PheCys: 0.431 ± 0.148
2.338PheAsp: 2.338 ± 0.434
2.707PheGlu: 2.707 ± 0.405
0.8PhePhe: 0.8 ± 0.253
3.445PheGly: 3.445 ± 0.539
0.492PheHis: 0.492 ± 0.149
1.784PheIle: 1.784 ± 0.302
1.292PheLys: 1.292 ± 0.25
2.338PheLeu: 2.338 ± 0.49
0.554PheMet: 0.554 ± 0.162
1.723PheAsn: 1.723 ± 0.299
2.215PhePro: 2.215 ± 0.392
1.107PheGln: 1.107 ± 0.273
2.276PheArg: 2.276 ± 0.33
2.338PheSer: 2.338 ± 0.471
1.969PheThr: 1.969 ± 0.339
2.215PheVal: 2.215 ± 0.386
0.923PheTrp: 0.923 ± 0.263
0.677PheTyr: 0.677 ± 0.164
0.0PheXaa: 0.0 ± 0.0
Gly
6.091GlyAla: 6.091 ± 0.802
0.861GlyCys: 0.861 ± 0.204
6.337GlyAsp: 6.337 ± 0.701
4.983GlyGlu: 4.983 ± 0.79
2.584GlyPhe: 2.584 ± 0.463
10.767GlyGly: 10.767 ± 2.958
1.907GlyHis: 1.907 ± 0.307
5.045GlyIle: 5.045 ± 0.732
4.737GlyLys: 4.737 ± 0.491
7.137GlyLeu: 7.137 ± 1.02
2.03GlyMet: 2.03 ± 0.298
2.953GlyAsn: 2.953 ± 0.523
4.737GlyPro: 4.737 ± 0.972
2.83GlyGln: 2.83 ± 0.534
4.491GlyArg: 4.491 ± 0.451
5.291GlySer: 5.291 ± 0.768
5.291GlyThr: 5.291 ± 0.773
5.599GlyVal: 5.599 ± 0.677
1.907GlyTrp: 1.907 ± 0.353
2.953GlyTyr: 2.953 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.6HisAla: 1.6 ± 0.386
0.246HisCys: 0.246 ± 0.127
1.415HisAsp: 1.415 ± 0.305
1.538HisGlu: 1.538 ± 0.345
0.615HisPhe: 0.615 ± 0.19
1.538HisGly: 1.538 ± 0.393
0.738HisHis: 0.738 ± 0.211
1.107HisIle: 1.107 ± 0.233
1.107HisLys: 1.107 ± 0.238
1.354HisLeu: 1.354 ± 0.244
0.308HisMet: 0.308 ± 0.141
0.492HisAsn: 0.492 ± 0.166
1.415HisPro: 1.415 ± 0.276
1.107HisGln: 1.107 ± 0.234
1.415HisArg: 1.415 ± 0.301
0.861HisSer: 0.861 ± 0.323
0.738HisThr: 0.738 ± 0.241
0.8HisVal: 0.8 ± 0.203
0.431HisTrp: 0.431 ± 0.158
0.677HisTyr: 0.677 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
4.676IleAla: 4.676 ± 0.651
0.431IleCys: 0.431 ± 0.164
3.814IleAsp: 3.814 ± 0.492
4.676IleGlu: 4.676 ± 0.479
1.354IlePhe: 1.354 ± 0.34
3.999IleGly: 3.999 ± 0.673
0.984IleHis: 0.984 ± 0.221
1.6IleIle: 1.6 ± 0.317
2.83IleLys: 2.83 ± 0.357
3.568IleLeu: 3.568 ± 0.413
0.369IleMet: 0.369 ± 0.151
2.092IleAsn: 2.092 ± 0.356
3.076IlePro: 3.076 ± 0.398
1.6IleGln: 1.6 ± 0.42
3.384IleArg: 3.384 ± 0.471
3.261IleSer: 3.261 ± 0.371
3.568IleThr: 3.568 ± 0.461
3.322IleVal: 3.322 ± 0.472
0.984IleTrp: 0.984 ± 0.199
1.292IleTyr: 1.292 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
4.368LysAla: 4.368 ± 0.699
0.369LysCys: 0.369 ± 0.152
3.015LysAsp: 3.015 ± 0.567
3.076LysGlu: 3.076 ± 0.452
1.292LysPhe: 1.292 ± 0.309
4.122LysGly: 4.122 ± 0.532
0.861LysHis: 0.861 ± 0.218
2.707LysIle: 2.707 ± 0.401
3.691LysLys: 3.691 ± 0.581
3.753LysLeu: 3.753 ± 0.542
1.046LysMet: 1.046 ± 0.242
1.538LysAsn: 1.538 ± 0.265
3.199LysPro: 3.199 ± 0.513
1.723LysGln: 1.723 ± 0.359
2.892LysArg: 2.892 ± 0.481
3.076LysSer: 3.076 ± 0.533
3.199LysThr: 3.199 ± 0.494
3.691LysVal: 3.691 ± 0.546
1.046LysTrp: 1.046 ± 0.314
1.169LysTyr: 1.169 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
8.49LeuAla: 8.49 ± 0.733
0.923LeuCys: 0.923 ± 0.272
4.368LeuAsp: 4.368 ± 0.469
5.168LeuGlu: 5.168 ± 0.685
2.646LeuPhe: 2.646 ± 0.402
5.845LeuGly: 5.845 ± 0.595
1.846LeuHis: 1.846 ± 0.342
4.799LeuIle: 4.799 ± 0.541
4.122LeuLys: 4.122 ± 0.661
5.291LeuLeu: 5.291 ± 0.618
2.769LeuMet: 2.769 ± 0.406
2.892LeuAsn: 2.892 ± 0.424
4.491LeuPro: 4.491 ± 0.477
1.846LeuGln: 1.846 ± 0.384
4.491LeuArg: 4.491 ± 0.511
5.045LeuSer: 5.045 ± 0.687
5.414LeuThr: 5.414 ± 0.654
4.43LeuVal: 4.43 ± 0.637
1.477LeuTrp: 1.477 ± 0.29
2.092LeuTyr: 2.092 ± 0.451
0.0LeuXaa: 0.0 ± 0.0
Met
2.153MetAla: 2.153 ± 0.422
0.062MetCys: 0.062 ± 0.068
1.107MetAsp: 1.107 ± 0.277
1.661MetGlu: 1.661 ± 0.332
0.615MetPhe: 0.615 ± 0.221
1.661MetGly: 1.661 ± 0.313
0.615MetHis: 0.615 ± 0.185
1.046MetIle: 1.046 ± 0.298
1.477MetLys: 1.477 ± 0.288
1.354MetLeu: 1.354 ± 0.33
0.738MetMet: 0.738 ± 0.222
0.8MetAsn: 0.8 ± 0.253
0.923MetPro: 0.923 ± 0.257
0.861MetGln: 0.861 ± 0.272
1.477MetArg: 1.477 ± 0.29
1.846MetSer: 1.846 ± 0.342
2.03MetThr: 2.03 ± 0.321
1.354MetVal: 1.354 ± 0.297
0.185MetTrp: 0.185 ± 0.104
0.8MetTyr: 0.8 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
3.199AsnAla: 3.199 ± 0.479
0.431AsnCys: 0.431 ± 0.194
1.969AsnAsp: 1.969 ± 0.302
1.969AsnGlu: 1.969 ± 0.388
0.861AsnPhe: 0.861 ± 0.231
3.999AsnGly: 3.999 ± 0.561
0.738AsnHis: 0.738 ± 0.186
1.23AsnIle: 1.23 ± 0.306
1.723AsnLys: 1.723 ± 0.327
2.707AsnLeu: 2.707 ± 0.457
0.861AsnMet: 0.861 ± 0.194
0.861AsnAsn: 0.861 ± 0.209
2.584AsnPro: 2.584 ± 0.379
1.23AsnGln: 1.23 ± 0.204
2.215AsnArg: 2.215 ± 0.336
1.661AsnSer: 1.661 ± 0.349
2.153AsnThr: 2.153 ± 0.351
2.338AsnVal: 2.338 ± 0.462
0.677AsnTrp: 0.677 ± 0.186
0.861AsnTyr: 0.861 ± 0.215
0.0AsnXaa: 0.0 ± 0.0
Pro
5.106ProAla: 5.106 ± 0.575
0.554ProCys: 0.554 ± 0.156
3.937ProAsp: 3.937 ± 0.549
4.061ProGlu: 4.061 ± 0.596
2.276ProPhe: 2.276 ± 0.376
4.799ProGly: 4.799 ± 0.575
0.738ProHis: 0.738 ± 0.221
2.892ProIle: 2.892 ± 0.383
2.892ProLys: 2.892 ± 0.429
3.138ProLeu: 3.138 ± 0.512
0.861ProMet: 0.861 ± 0.255
2.646ProAsn: 2.646 ± 0.412
2.338ProPro: 2.338 ± 0.636
2.584ProGln: 2.584 ± 0.571
3.199ProArg: 3.199 ± 0.5
2.953ProSer: 2.953 ± 0.381
3.876ProThr: 3.876 ± 0.457
4.245ProVal: 4.245 ± 0.546
1.046ProTrp: 1.046 ± 0.315
1.415ProTyr: 1.415 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
4.676GlnAla: 4.676 ± 0.663
0.246GlnCys: 0.246 ± 0.131
2.03GlnAsp: 2.03 ± 0.34
1.477GlnGlu: 1.477 ± 0.363
1.046GlnPhe: 1.046 ± 0.294
3.876GlnGly: 3.876 ± 1.415
0.984GlnHis: 0.984 ± 0.26
2.646GlnIle: 2.646 ± 0.398
1.477GlnLys: 1.477 ± 0.4
3.322GlnLeu: 3.322 ± 0.498
1.169GlnMet: 1.169 ± 0.246
0.861GlnAsn: 0.861 ± 0.183
1.107GlnPro: 1.107 ± 0.262
1.784GlnGln: 1.784 ± 0.462
1.969GlnArg: 1.969 ± 0.453
1.969GlnSer: 1.969 ± 0.396
1.477GlnThr: 1.477 ± 0.332
2.707GlnVal: 2.707 ± 0.455
0.738GlnTrp: 0.738 ± 0.234
1.169GlnTyr: 1.169 ± 0.287
0.0GlnXaa: 0.0 ± 0.0
Arg
5.291ArgAla: 5.291 ± 0.59
0.861ArgCys: 0.861 ± 0.333
3.568ArgAsp: 3.568 ± 0.465
4.676ArgGlu: 4.676 ± 0.636
2.83ArgPhe: 2.83 ± 0.599
3.876ArgGly: 3.876 ± 0.516
0.984ArgHis: 0.984 ± 0.259
3.384ArgIle: 3.384 ± 0.468
3.507ArgLys: 3.507 ± 0.506
5.599ArgLeu: 5.599 ± 0.683
1.907ArgMet: 1.907 ± 0.351
1.723ArgAsn: 1.723 ± 0.31
2.215ArgPro: 2.215 ± 0.363
2.03ArgGln: 2.03 ± 0.33
4.737ArgArg: 4.737 ± 0.6
2.646ArgSer: 2.646 ± 0.41
2.522ArgThr: 2.522 ± 0.334
4.43ArgVal: 4.43 ± 0.513
1.415ArgTrp: 1.415 ± 0.288
2.03ArgTyr: 2.03 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
5.106SerAla: 5.106 ± 0.572
0.554SerCys: 0.554 ± 0.182
3.261SerAsp: 3.261 ± 0.558
3.568SerGlu: 3.568 ± 0.466
2.215SerPhe: 2.215 ± 0.487
5.291SerGly: 5.291 ± 0.852
0.677SerHis: 0.677 ± 0.18
2.399SerIle: 2.399 ± 0.432
2.461SerLys: 2.461 ± 0.361
4.307SerLeu: 4.307 ± 0.594
1.292SerMet: 1.292 ± 0.265
1.354SerAsn: 1.354 ± 0.28
3.937SerPro: 3.937 ± 0.437
2.276SerGln: 2.276 ± 0.354
3.691SerArg: 3.691 ± 0.417
4.122SerSer: 4.122 ± 0.72
3.199SerThr: 3.199 ± 0.464
4.061SerVal: 4.061 ± 0.518
0.984SerTrp: 0.984 ± 0.275
1.477SerTyr: 1.477 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
4.86ThrAla: 4.86 ± 0.436
0.492ThrCys: 0.492 ± 0.146
3.384ThrAsp: 3.384 ± 0.515
3.384ThrGlu: 3.384 ± 0.396
2.522ThrPhe: 2.522 ± 0.408
5.599ThrGly: 5.599 ± 0.74
1.107ThrHis: 1.107 ± 0.294
3.015ThrIle: 3.015 ± 0.424
2.83ThrLys: 2.83 ± 0.432
4.922ThrLeu: 4.922 ± 0.503
0.8ThrMet: 0.8 ± 0.238
1.661ThrAsn: 1.661 ± 0.274
4.676ThrPro: 4.676 ± 0.653
2.461ThrGln: 2.461 ± 0.312
2.769ThrArg: 2.769 ± 0.379
3.507ThrSer: 3.507 ± 0.515
2.892ThrThr: 2.892 ± 0.512
4.245ThrVal: 4.245 ± 0.442
1.046ThrTrp: 1.046 ± 0.271
1.723ThrTyr: 1.723 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
6.152ValAla: 6.152 ± 0.725
0.615ValCys: 0.615 ± 0.202
5.229ValAsp: 5.229 ± 0.537
4.676ValGlu: 4.676 ± 0.57
2.215ValPhe: 2.215 ± 0.394
5.229ValGly: 5.229 ± 0.644
1.107ValHis: 1.107 ± 0.322
3.691ValIle: 3.691 ± 0.438
3.63ValLys: 3.63 ± 0.487
5.66ValLeu: 5.66 ± 0.616
1.292ValMet: 1.292 ± 0.275
2.461ValAsn: 2.461 ± 0.392
3.691ValPro: 3.691 ± 0.587
2.092ValGln: 2.092 ± 0.334
4.061ValArg: 4.061 ± 0.615
3.876ValSer: 3.876 ± 0.467
4.368ValThr: 4.368 ± 0.512
5.414ValVal: 5.414 ± 0.698
1.723ValTrp: 1.723 ± 0.324
1.846ValTyr: 1.846 ± 0.423
0.0ValXaa: 0.0 ± 0.0
Trp
2.03TrpAla: 2.03 ± 0.338
0.431TrpCys: 0.431 ± 0.162
1.477TrpAsp: 1.477 ± 0.283
1.661TrpGlu: 1.661 ± 0.312
0.554TrpPhe: 0.554 ± 0.195
1.415TrpGly: 1.415 ± 0.364
0.554TrpHis: 0.554 ± 0.184
0.923TrpIle: 0.923 ± 0.279
0.861TrpLys: 0.861 ± 0.228
0.923TrpLeu: 0.923 ± 0.299
0.615TrpMet: 0.615 ± 0.194
1.107TrpAsn: 1.107 ± 0.363
0.923TrpPro: 0.923 ± 0.252
1.169TrpGln: 1.169 ± 0.316
0.984TrpArg: 0.984 ± 0.178
1.23TrpSer: 1.23 ± 0.297
1.23TrpThr: 1.23 ± 0.292
1.477TrpVal: 1.477 ± 0.328
0.984TrpTrp: 0.984 ± 0.31
0.308TrpTyr: 0.308 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.092TyrAla: 2.092 ± 0.412
0.431TyrCys: 0.431 ± 0.168
2.461TyrAsp: 2.461 ± 0.335
2.399TyrGlu: 2.399 ± 0.461
0.677TyrPhe: 0.677 ± 0.22
2.522TyrGly: 2.522 ± 0.374
0.369TyrHis: 0.369 ± 0.183
1.23TyrIle: 1.23 ± 0.208
0.554TyrLys: 0.554 ± 0.202
2.83TyrLeu: 2.83 ± 0.409
0.308TyrMet: 0.308 ± 0.152
0.738TyrAsn: 0.738 ± 0.188
1.354TyrPro: 1.354 ± 0.329
0.861TyrGln: 0.861 ± 0.264
1.907TyrArg: 1.907 ± 0.33
1.784TyrSer: 1.784 ± 0.323
1.661TyrThr: 1.661 ± 0.361
2.584TyrVal: 2.584 ± 0.476
0.431TyrTrp: 0.431 ± 0.15
1.354TyrTyr: 1.354 ± 0.275
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (16255 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski