Salmonella phage vB_SenS_phi135

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BZN7|A0A3G3BZN7_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_phi135 OX=2420239 GN=phi135_19 PE=4 SV=1
MM1 pKa = 7.19ATYY4 pKa = 10.6FEE6 pKa = 5.55DD7 pKa = 3.3LTKK10 pKa = 11.12AFDD13 pKa = 3.37TALAAFGTDD22 pKa = 2.97NDD24 pKa = 3.73IMVALEE30 pKa = 4.82NIDD33 pKa = 4.56APTSTDD39 pKa = 3.13VPYY42 pKa = 10.45LASYY46 pKa = 9.38MLLSDD51 pKa = 4.41TEE53 pKa = 4.16QADD56 pKa = 4.43LFWTEE61 pKa = 3.66QRR63 pKa = 11.84AGIYY67 pKa = 9.69QVDD70 pKa = 3.89INVGSTLGSAPLNRR84 pKa = 11.84LADD87 pKa = 3.71KK88 pKa = 11.28LNATFAAGNCFSRR101 pKa = 11.84NEE103 pKa = 3.63ICAEE107 pKa = 4.01VQSVSLGPLIVEE119 pKa = 4.42NGWAKK124 pKa = 10.54RR125 pKa = 11.84PLSINFIAFTARR137 pKa = 11.84IRR139 pKa = 3.58

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BZL8|A0A3G3BZL8_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_phi135 OX=2420239 GN=phi135_21 PE=4 SV=1
MM1 pKa = 7.09VCASLTLINAFMVSSPLVGVDD22 pKa = 2.62IFNFEE27 pKa = 3.33HH28 pKa = 7.17SYY30 pKa = 10.84RR31 pKa = 11.84SNQILYY37 pKa = 10.29RR38 pKa = 11.84DD39 pKa = 4.28DD40 pKa = 3.52KK41 pKa = 11.4KK42 pKa = 10.9KK43 pKa = 10.69APKK46 pKa = 10.22GAFSHH51 pKa = 6.69SSILLRR57 pKa = 11.84YY58 pKa = 8.38FRR60 pKa = 11.84NFSRR64 pKa = 11.84LSIASAKK71 pKa = 9.74SVPVSLCPRR80 pKa = 11.84KK81 pKa = 9.48PIHH84 pKa = 6.52NSFFNACCSAGSRR97 pKa = 11.84ISNALRR103 pKa = 11.84TSYY106 pKa = 10.25TSQVMLSPSVFVIVRR121 pKa = 11.84LYY123 pKa = 11.25LLVIPASPVKK133 pKa = 10.64

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13261

39

1032

224.8

24.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.577 ± 0.524

0.965 ± 0.128

6.033 ± 0.179

6.628 ± 0.424

3.831 ± 0.157

7.812 ± 0.312

1.682 ± 0.148

4.864 ± 0.198

5.859 ± 0.347

7.865 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.135

4.336 ± 0.246

3.974 ± 0.204

3.65 ± 0.224

5.671 ± 0.218

6.191 ± 0.278

6.334 ± 0.338

7.398 ± 0.302

1.44 ± 0.148

3.348 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski