Nematostella vectensis (Starlet sea anemone)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria; Edwardsiidae; Nematostella

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7T6T1|A7T6T1_NEMVE Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g148194 PE=4 SV=1
MM1 pKa = 7.55LFQRR5 pKa = 11.84GRR7 pKa = 11.84YY8 pKa = 6.19FTQGSFKK15 pKa = 10.82NVGFAYY21 pKa = 9.47MKK23 pKa = 10.11VFVMMIAVVNVAAVVVSVDD42 pKa = 3.03AAADD46 pKa = 3.6VSVDD50 pKa = 3.02IATVAVNVDD59 pKa = 3.34VVAVFVNVDD68 pKa = 2.76VDD70 pKa = 3.9AVVVNVGVAAVVAVSNVCVAAVVVNVATVVVNVDD104 pKa = 3.08AAAVVVGVDD113 pKa = 3.36VATFAADD120 pKa = 3.07VDD122 pKa = 4.22VAAVVVYY129 pKa = 10.77VDD131 pKa = 4.49FAAVVVNVDD140 pKa = 3.24VAAVIVNVDD149 pKa = 3.26VAVVVRR155 pKa = 11.84NVDD158 pKa = 3.02VAAAIFVNVDD168 pKa = 3.17VEE170 pKa = 4.84TVGVNVDD177 pKa = 3.08VAAAVVNDD185 pKa = 3.97DD186 pKa = 3.64VASVVVNVDD195 pKa = 2.42VFAAVVNVAAVVVSVDD211 pKa = 3.2AAADD215 pKa = 3.95LLLMLQQLLLVLMLLQIQNDD235 pKa = 3.76YY236 pKa = 11.17NALNEE241 pKa = 4.34CNSCRR246 pKa = 11.84VAMDD250 pKa = 3.93SSNSSILILFYY261 pKa = 10.74HH262 pKa = 5.51SQCVSMHH269 pKa = 7.08RR270 pKa = 11.84IFLYY274 pKa = 10.58CSHH277 pKa = 6.51TPILKK282 pKa = 7.9MTTTHH287 pKa = 7.06SIRR290 pKa = 11.84VTAVGSQWNLVIEE303 pKa = 4.51VSS305 pKa = 3.38

Molecular weight: 31.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7T7G3|A7T7G3_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g148833 PE=4 SV=1
HHH2 pKa = 7.02NRR4 pKa = 11.84LPGLTSINTNRR15 pKa = 11.84LPGLTSINTNRR26 pKa = 11.84LPGLTSINTNRR37 pKa = 11.84LPGLTSINTNRR48 pKa = 11.84LPGLTSINTNRR59 pKa = 11.84LPGLTSINTNRR70 pKa = 11.84LPGLTSINTNRR81 pKa = 11.84LPGLTSINTNRR92 pKa = 11.84LPGLTSINTNRR103 pKa = 11.84LPGLTSINTNRR114 pKa = 11.84LPGLTSINTNRR125 pKa = 11.84LPGLTSINTNRR136 pKa = 11.84LPG

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24428

16

24444

8260506

23

8745

337.9

37.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.417 ± 0.018

2.432 ± 0.019

5.257 ± 0.016

5.981 ± 0.023

4.044 ± 0.014

6.18 ± 0.022

2.697 ± 0.014

5.418 ± 0.016

6.151 ± 0.019

9.24 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.008

4.28 ± 0.015

4.916 ± 0.019

3.898 ± 0.013

5.525 ± 0.019

7.765 ± 0.023

5.976 ± 0.018

6.718 ± 0.017

1.292 ± 0.008

3.423 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski