Amino acid dipepetide frequency for Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.511AlaAla: 12.511 ± 0.289	0.809AlaCys: 0.809 ± 0.047	3.319AlaAsp: 3.319 ± 0.087	7.323AlaGlu: 7.323 ± 0.133	2.523AlaPhe: 2.523 ± 0.068	7.923AlaGly: 7.923 ± 0.19	1.325AlaHis: 1.325 ± 0.062	5.893AlaIle: 5.893 ± 0.117	4.706AlaLys: 4.706 ± 0.107	10.926AlaLeu: 10.926 ± 0.19	2.248AlaMet: 2.248 ± 0.073	1.821AlaAsn: 1.821 ± 0.068	3.59AlaPro: 3.59 ± 0.088	1.645AlaGln: 1.645 ± 0.058	9.219AlaArg: 9.219 ± 0.172	6.28AlaSer: 6.28 ± 0.14	4.184AlaThr: 4.184 ± 0.106	9.332AlaVal: 9.332 ± 0.165	1.369AlaTrp: 1.369 ± 0.07	3.668AlaTyr: 3.668 ± 0.101	0.0AlaXaa: 0.0 ± 0.0
Cys	0.527CysAla: 0.527 ± 0.038	0.136CysCys: 0.136 ± 0.017	0.265CysAsp: 0.265 ± 0.026	0.398CysGlu: 0.398 ± 0.026	0.185CysPhe: 0.185 ± 0.02	1.06CysGly: 1.06 ± 0.053	0.191CysHis: 0.191 ± 0.03	0.669CysIle: 0.669 ± 0.042	0.387CysLys: 0.387 ± 0.033	0.616CysLeu: 0.616 ± 0.042	0.276CysMet: 0.276 ± 0.027	0.289CysAsn: 0.289 ± 0.026	0.863CysPro: 0.863 ± 0.058	0.176CysGln: 0.176 ± 0.021	0.925CysArg: 0.925 ± 0.045	0.889CysSer: 0.889 ± 0.04	0.449CysThr: 0.449 ± 0.031	0.489CysVal: 0.489 ± 0.035	0.16CysTrp: 0.16 ± 0.018	0.269CysTyr: 0.269 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.959AspAla: 3.959 ± 0.1	0.362AspCys: 0.362 ± 0.029	1.612AspAsp: 1.612 ± 0.058	3.57AspGlu: 3.57 ± 0.085	1.174AspPhe: 1.174 ± 0.054	2.892AspGly: 2.892 ± 0.095	0.614AspHis: 0.614 ± 0.039	3.192AspIle: 3.192 ± 0.09	1.979AspLys: 1.979 ± 0.072	3.561AspLeu: 3.561 ± 0.078	0.936AspMet: 0.936 ± 0.047	1.04AspAsn: 1.04 ± 0.055	3.092AspPro: 3.092 ± 0.093	0.631AspGln: 0.631 ± 0.041	2.459AspArg: 2.459 ± 0.086	1.858AspSer: 1.858 ± 0.067	2.172AspThr: 2.172 ± 0.077	4.095AspVal: 4.095 ± 0.095	0.571AspTrp: 0.571 ± 0.038	2.101AspTyr: 2.101 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	9.581GluAla: 9.581 ± 0.183	0.507GluCys: 0.507 ± 0.034	3.066GluAsp: 3.066 ± 0.086	7.756GluGlu: 7.756 ± 0.196	1.934GluPhe: 1.934 ± 0.062	4.648GluGly: 4.648 ± 0.091	1.552GluHis: 1.552 ± 0.063	4.035GluIle: 4.035 ± 0.117	5.315GluLys: 5.315 ± 0.133	8.968GluLeu: 8.968 ± 0.148	1.249GluMet: 1.249 ± 0.054	1.91GluAsn: 1.91 ± 0.064	3.768GluPro: 3.768 ± 0.106	1.907GluGln: 1.907 ± 0.074	5.302GluArg: 5.302 ± 0.119	2.708GluSer: 2.708 ± 0.085	3.132GluThr: 3.132 ± 0.084	5.74GluVal: 5.74 ± 0.122	0.965GluTrp: 0.965 ± 0.049	2.761GluTyr: 2.761 ± 0.084	0.0GluXaa: 0.0 ± 0.0
Phe	2.379PheAla: 2.379 ± 0.086	0.271PheCys: 0.271 ± 0.024	1.358PheAsp: 1.358 ± 0.07	1.901PheGlu: 1.901 ± 0.062	0.86PhePhe: 0.86 ± 0.047	1.856PheGly: 1.856 ± 0.064	0.631PheHis: 0.631 ± 0.036	1.979PheIle: 1.979 ± 0.071	1.227PheLys: 1.227 ± 0.065	2.554PheLeu: 2.554 ± 0.084	0.651PheMet: 0.651 ± 0.038	0.907PheAsn: 0.907 ± 0.049	1.016PhePro: 1.016 ± 0.047	0.585PheGln: 0.585 ± 0.036	1.874PheArg: 1.874 ± 0.068	1.667PheSer: 1.667 ± 0.067	1.725PheThr: 1.725 ± 0.062	2.036PheVal: 2.036 ± 0.064	0.342PheTrp: 0.342 ± 0.03	1.3PheTyr: 1.3 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	5.876GlyAla: 5.876 ± 0.139	0.974GlyCys: 0.974 ± 0.05	3.395GlyAsp: 3.395 ± 0.084	5.527GlyGlu: 5.527 ± 0.124	3.177GlyPhe: 3.177 ± 0.086	5.718GlyGly: 5.718 ± 0.136	1.236GlyHis: 1.236 ± 0.052	4.955GlyIle: 4.955 ± 0.104	3.73GlyLys: 3.73 ± 0.103	8.741GlyLeu: 8.741 ± 0.169	1.892GlyMet: 1.892 ± 0.063	1.529GlyAsn: 1.529 ± 0.062	2.821GlyPro: 2.821 ± 0.085	1.325GlyGln: 1.325 ± 0.057	5.878GlyArg: 5.878 ± 0.12	4.286GlySer: 4.286 ± 0.111	2.99GlyThr: 2.99 ± 0.092	7.416GlyVal: 7.416 ± 0.147	1.183GlyTrp: 1.183 ± 0.054	3.555GlyTyr: 3.555 ± 0.099	0.0GlyXaa: 0.0 ± 0.0
His	1.592HisAla: 1.592 ± 0.058	0.182HisCys: 0.182 ± 0.022	0.738HisAsp: 0.738 ± 0.042	1.096HisGlu: 1.096 ± 0.054	0.385HisPhe: 0.385 ± 0.031	1.872HisGly: 1.872 ± 0.068	0.418HisHis: 0.418 ± 0.032	1.192HisIle: 1.192 ± 0.055	0.56HisLys: 0.56 ± 0.035	1.532HisLeu: 1.532 ± 0.064	0.425HisMet: 0.425 ± 0.03	0.413HisAsn: 0.413 ± 0.032	1.196HisPro: 1.196 ± 0.051	0.318HisGln: 0.318 ± 0.026	1.401HisArg: 1.401 ± 0.056	0.945HisSer: 0.945 ± 0.047	0.865HisThr: 0.865 ± 0.042	1.912HisVal: 1.912 ± 0.063	0.216HisTrp: 0.216 ± 0.023	0.645HisTyr: 0.645 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	7.643IleAla: 7.643 ± 0.13	0.425IleCys: 0.425 ± 0.027	3.45IleAsp: 3.45 ± 0.09	4.924IleGlu: 4.924 ± 0.126	1.289IlePhe: 1.289 ± 0.055	4.279IleGly: 4.279 ± 0.109	1.172IleHis: 1.172 ± 0.043	3.753IleIle: 3.753 ± 0.104	2.156IleLys: 2.156 ± 0.081	6.167IleLeu: 6.167 ± 0.117	1.098IleMet: 1.098 ± 0.045	1.352IleAsn: 1.352 ± 0.061	3.201IlePro: 3.201 ± 0.091	1.003IleGln: 1.003 ± 0.05	4.07IleArg: 4.07 ± 0.094	2.645IleSer: 2.645 ± 0.076	2.648IleThr: 2.648 ± 0.097	7.607IleVal: 7.607 ± 0.12	0.476IleTrp: 0.476 ± 0.032	2.356IleTyr: 2.356 ± 0.073	0.0IleXaa: 0.0 ± 0.0
Lys	4.477LysAla: 4.477 ± 0.121	0.347LysCys: 0.347 ± 0.03	1.252LysAsp: 1.252 ± 0.06	2.485LysGlu: 2.485 ± 0.082	0.949LysPhe: 0.949 ± 0.047	2.95LysGly: 2.95 ± 0.089	0.863LysHis: 0.863 ± 0.042	2.821LysIle: 2.821 ± 0.09	2.305LysLys: 2.305 ± 0.089	7.198LysLeu: 7.198 ± 0.149	0.883LysMet: 0.883 ± 0.045	0.758LysAsn: 0.758 ± 0.044	3.641LysPro: 3.641 ± 0.099	1.054LysGln: 1.054 ± 0.055	3.33LysArg: 3.33 ± 0.113	1.77LysSer: 1.77 ± 0.064	2.465LysThr: 2.465 ± 0.077	3.386LysVal: 3.386 ± 0.095	0.547LysTrp: 0.547 ± 0.037	1.592LysTyr: 1.592 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	13.45LeuAla: 13.45 ± 0.244	0.787LeuCys: 0.787 ± 0.042	5.224LeuAsp: 5.224 ± 0.121	9.619LeuGlu: 9.619 ± 0.171	2.51LeuPhe: 2.51 ± 0.082	8.99LeuGly: 8.99 ± 0.157	2.23LeuHis: 2.23 ± 0.069	5.173LeuIle: 5.173 ± 0.115	4.346LeuLys: 4.346 ± 0.111	12.485LeuLeu: 12.485 ± 0.209	1.836LeuMet: 1.836 ± 0.06	2.223LeuAsn: 2.223 ± 0.072	5.011LeuPro: 5.011 ± 0.127	2.485LeuGln: 2.485 ± 0.081	9.235LeuArg: 9.235 ± 0.173	5.958LeuSer: 5.958 ± 0.12	4.226LeuThr: 4.226 ± 0.118	10.266LeuVal: 10.266 ± 0.164	1.163LeuTrp: 1.163 ± 0.051	4.168LeuTyr: 4.168 ± 0.104	0.0LeuXaa: 0.0 ± 0.0
Met	2.363MetAla: 2.363 ± 0.07	0.165MetCys: 0.165 ± 0.019	0.751MetAsp: 0.751 ± 0.04	1.529MetGlu: 1.529 ± 0.058	0.556MetPhe: 0.556 ± 0.039	1.598MetGly: 1.598 ± 0.053	0.418MetHis: 0.418 ± 0.035	1.12MetIle: 1.12 ± 0.051	0.963MetLys: 0.963 ± 0.048	2.768MetLeu: 2.768 ± 0.078	0.378MetMet: 0.378 ± 0.03	0.442MetAsn: 0.442 ± 0.032	1.278MetPro: 1.278 ± 0.055	0.565MetGln: 0.565 ± 0.035	1.409MetArg: 1.409 ± 0.057	1.205MetSer: 1.205 ± 0.056	0.94MetThr: 0.94 ± 0.048	1.834MetVal: 1.834 ± 0.071	0.171MetTrp: 0.171 ± 0.02	0.622MetTyr: 0.622 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	1.921AsnAla: 1.921 ± 0.064	0.285AsnCys: 0.285 ± 0.026	0.823AsnAsp: 0.823 ± 0.046	1.107AsnGlu: 1.107 ± 0.054	0.462AsnPhe: 0.462 ± 0.032	1.654AsnGly: 1.654 ± 0.065	0.322AsnHis: 0.322 ± 0.025	1.976AsnIle: 1.976 ± 0.067	0.938AsnLys: 0.938 ± 0.044	2.072AsnLeu: 2.072 ± 0.075	0.625AsnMet: 0.625 ± 0.035	0.578AsnAsn: 0.578 ± 0.036	1.99AsnPro: 1.99 ± 0.061	0.331AsnGln: 0.331 ± 0.024	1.138AsnArg: 1.138 ± 0.05	0.983AsnSer: 0.983 ± 0.056	1.338AsnThr: 1.338 ± 0.059	2.019AsnVal: 2.019 ± 0.072	0.229AsnTrp: 0.229 ± 0.024	0.871AsnTyr: 0.871 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	4.11ProAla: 4.11 ± 0.111	0.465ProCys: 0.465 ± 0.029	2.403ProAsp: 2.403 ± 0.071	4.144ProGlu: 4.144 ± 0.091	1.374ProPhe: 1.374 ± 0.064	4.622ProGly: 4.622 ± 0.122	0.934ProHis: 0.934 ± 0.045	2.519ProIle: 2.519 ± 0.078	1.712ProLys: 1.712 ± 0.066	5.195ProLeu: 5.195 ± 0.101	0.805ProMet: 0.805 ± 0.044	1.005ProAsn: 1.005 ± 0.045	3.192ProPro: 3.192 ± 0.1	1.1ProGln: 1.1 ± 0.051	4.371ProArg: 4.371 ± 0.109	3.108ProSer: 3.108 ± 0.083	2.012ProThr: 2.012 ± 0.062	4.771ProVal: 4.771 ± 0.111	0.869ProTrp: 0.869 ± 0.046	1.914ProTyr: 1.914 ± 0.068	0.0ProXaa: 0.0 ± 0.0
Gln	2.163GlnAla: 2.163 ± 0.075	0.165GlnCys: 0.165 ± 0.022	0.562GlnAsp: 0.562 ± 0.03	1.169GlnGlu: 1.169 ± 0.06	0.413GlnPhe: 0.413 ± 0.028	1.536GlnGly: 1.536 ± 0.059	0.505GlnHis: 0.505 ± 0.038	0.967GlnIle: 0.967 ± 0.054	0.827GlnLys: 0.827 ± 0.046	2.941GlnLeu: 2.941 ± 0.074	0.393GlnMet: 0.393 ± 0.029	0.387GlnAsn: 0.387 ± 0.027	1.383GlnPro: 1.383 ± 0.066	0.791GlnGln: 0.791 ± 0.05	1.734GlnArg: 1.734 ± 0.071	0.883GlnSer: 0.883 ± 0.048	0.787GlnThr: 0.787 ± 0.05	1.583GlnVal: 1.583 ± 0.058	0.185GlnTrp: 0.185 ± 0.022	0.616GlnTyr: 0.616 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	6.051ArgAla: 6.051 ± 0.127	0.891ArgCys: 0.891 ± 0.051	3.248ArgAsp: 3.248 ± 0.098	6.385ArgGlu: 6.385 ± 0.134	2.205ArgPhe: 2.205 ± 0.074	6.476ArgGly: 6.476 ± 0.137	1.378ArgHis: 1.378 ± 0.049	5.631ArgIle: 5.631 ± 0.108	3.317ArgLys: 3.317 ± 0.103	10.268ArgLeu: 10.268 ± 0.187	1.627ArgMet: 1.627 ± 0.065	1.496ArgAsn: 1.496 ± 0.06	3.068ArgPro: 3.068 ± 0.081	1.523ArgGln: 1.523 ± 0.058	7.341ArgArg: 7.341 ± 0.166	3.899ArgSer: 3.899 ± 0.097	2.443ArgThr: 2.443 ± 0.076	7.094ArgVal: 7.094 ± 0.172	0.923ArgTrp: 0.923 ± 0.046	2.848ArgTyr: 2.848 ± 0.078	0.0ArgXaa: 0.0 ± 0.0
Ser	3.775SerAla: 3.775 ± 0.093	0.654SerCys: 0.654 ± 0.044	1.985SerAsp: 1.985 ± 0.074	3.161SerGlu: 3.161 ± 0.076	1.685SerPhe: 1.685 ± 0.072	4.217SerGly: 4.217 ± 0.111	0.907SerHis: 0.907 ± 0.048	4.055SerIle: 4.055 ± 0.11	2.528SerLys: 2.528 ± 0.071	6.207SerLeu: 6.207 ± 0.127	1.641SerMet: 1.641 ± 0.055	1.107SerAsn: 1.107 ± 0.05	2.697SerPro: 2.697 ± 0.095	1.265SerGln: 1.265 ± 0.057	4.446SerArg: 4.446 ± 0.109	3.392SerSer: 3.392 ± 0.111	2.523SerThr: 2.523 ± 0.075	4.024SerVal: 4.024 ± 0.106	0.763SerTrp: 0.763 ± 0.042	1.99SerTyr: 1.99 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	4.255ThrAla: 4.255 ± 0.098	0.469ThrCys: 0.469 ± 0.034	1.389ThrAsp: 1.389 ± 0.056	1.959ThrGlu: 1.959 ± 0.073	1.052ThrPhe: 1.052 ± 0.047	4.311ThrGly: 4.311 ± 0.103	0.767ThrHis: 0.767 ± 0.042	3.161ThrIle: 3.161 ± 0.103	1.343ThrLys: 1.343 ± 0.053	5.024ThrLeu: 5.024 ± 0.129	1.016ThrMet: 1.016 ± 0.045	0.845ThrAsn: 0.845 ± 0.049	2.745ThrPro: 2.745 ± 0.085	0.709ThrGln: 0.709 ± 0.036	3.188ThrArg: 3.188 ± 0.086	2.634ThrSer: 2.634 ± 0.083	2.185ThrThr: 2.185 ± 0.078	4.911ThrVal: 4.911 ± 0.117	0.534ThrTrp: 0.534 ± 0.04	1.498ThrTyr: 1.498 ± 0.082	0.0ThrXaa: 0.0 ± 0.0
Val	9.244ValAla: 9.244 ± 0.16	0.716ValCys: 0.716 ± 0.047	4.813ValAsp: 4.813 ± 0.104	9.386ValGlu: 9.386 ± 0.169	3.021ValPhe: 3.021 ± 0.094	5.484ValGly: 5.484 ± 0.118	1.534ValHis: 1.534 ± 0.059	5.255ValIle: 5.255 ± 0.13	4.775ValLys: 4.775 ± 0.121	9.208ValLeu: 9.208 ± 0.146	1.75ValMet: 1.75 ± 0.063	2.125ValAsn: 2.125 ± 0.069	3.739ValPro: 3.739 ± 0.092	1.503ValGln: 1.503 ± 0.061	6.276ValArg: 6.276 ± 0.136	4.833ValSer: 4.833 ± 0.11	3.986ValThr: 3.986 ± 0.093	9.246ValVal: 9.246 ± 0.174	0.967ValTrp: 0.967 ± 0.047	4.286ValTyr: 4.286 ± 0.11	0.0ValXaa: 0.0 ± 0.0
Trp	0.943TrpAla: 0.943 ± 0.047	0.14TrpCys: 0.14 ± 0.019	0.509TrpAsp: 0.509 ± 0.037	0.831TrpGlu: 0.831 ± 0.042	0.407TrpPhe: 0.407 ± 0.033	0.867TrpGly: 0.867 ± 0.05	0.271TrpHis: 0.271 ± 0.028	0.771TrpIle: 0.771 ± 0.042	0.556TrpLys: 0.556 ± 0.037	1.652TrpLeu: 1.652 ± 0.058	0.26TrpMet: 0.26 ± 0.025	0.293TrpAsn: 0.293 ± 0.027	0.52TrpPro: 0.52 ± 0.037	0.322TrpGln: 0.322 ± 0.026	1.309TrpArg: 1.309 ± 0.054	0.783TrpSer: 0.783 ± 0.045	0.427TrpThr: 0.427 ± 0.033	0.92TrpVal: 0.92 ± 0.047	0.213TrpTrp: 0.213 ± 0.022	0.407TrpTyr: 0.407 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.797TyrAla: 3.797 ± 0.095	0.469TyrCys: 0.469 ± 0.035	1.712TyrAsp: 1.712 ± 0.062	2.534TyrGlu: 2.534 ± 0.077	0.96TyrPhe: 0.96 ± 0.05	3.248TyrGly: 3.248 ± 0.091	0.634TyrHis: 0.634 ± 0.037	2.597TyrIle: 2.597 ± 0.072	1.403TyrLys: 1.403 ± 0.059	3.301TyrLeu: 3.301 ± 0.082	1.087TyrMet: 1.087 ± 0.044	1.14TyrAsn: 1.14 ± 0.044	1.812TyrPro: 1.812 ± 0.062	0.751TyrGln: 0.751 ± 0.05	3.208TyrArg: 3.208 ± 0.089	2.294TyrSer: 2.294 ± 0.065	2.497TyrThr: 2.497 ± 0.081	3.466TyrVal: 3.466 ± 0.088	0.558TyrTrp: 0.558 ± 0.043	1.601TyrTyr: 1.601 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1602 proteins (449830 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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