Roseibium hamelinense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Roseibium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562T0Y1|A0A562T0Y1_9RHOB Uncharacterized protein OS=Roseibium hamelinense OX=150831 GN=JM93_02471 PE=4 SV=1
MM1 pKa = 7.7PNIFEE6 pKa = 4.34TSDD9 pKa = 3.41AASGTSTSYY18 pKa = 11.32VLRR21 pKa = 11.84EE22 pKa = 3.95TGKK25 pKa = 10.09IAQGTQNAVFTTVQAHH41 pKa = 6.92LAPGDD46 pKa = 3.83DD47 pKa = 4.17SDD49 pKa = 4.67WFAIDD54 pKa = 4.31LKK56 pKa = 11.1AGQTYY61 pKa = 8.14TFAAVGTFDD70 pKa = 3.66GRR72 pKa = 11.84GRR74 pKa = 11.84LEE76 pKa = 4.34DD77 pKa = 4.73PYY79 pKa = 11.56LKK81 pKa = 10.91LLDD84 pKa = 3.83QSGSLIGEE92 pKa = 4.74DD93 pKa = 4.48DD94 pKa = 3.93DD95 pKa = 5.56AGPGAYY101 pKa = 9.76SAITFTAQTSGRR113 pKa = 11.84YY114 pKa = 8.3FLSVSAGEE122 pKa = 4.36AGLTGQYY129 pKa = 10.68GLSAVEE135 pKa = 4.1GTKK138 pKa = 10.41AVYY141 pKa = 10.4DD142 pKa = 3.67GDD144 pKa = 3.95MAAGALLRR152 pKa = 11.84PGVSWGTPGQPATVTYY168 pKa = 10.2GFRR171 pKa = 11.84DD172 pKa = 3.57TLNNLDD178 pKa = 3.91PEE180 pKa = 4.53YY181 pKa = 10.58AAEE184 pKa = 4.08VPEE187 pKa = 4.23TFEE190 pKa = 4.35QVTAAQMVAVEE201 pKa = 5.57AILATYY207 pKa = 10.37SEE209 pKa = 4.16IANIQFEE216 pKa = 4.53RR217 pKa = 11.84VNPDD221 pKa = 2.86GYY223 pKa = 9.63TDD225 pKa = 3.86DD226 pKa = 5.58ASMLFSNYY234 pKa = 9.14FSTTDD239 pKa = 3.21GAGAYY244 pKa = 9.84AYY246 pKa = 10.42FPGSDD251 pKa = 3.77ASDD254 pKa = 3.02RR255 pKa = 11.84WEE257 pKa = 4.23GDD259 pKa = 2.52VWLNTNSVSKK269 pKa = 11.14EE270 pKa = 3.71EE271 pKa = 4.59LPNGSYY277 pKa = 10.22SHH279 pKa = 6.84FAVMHH284 pKa = 6.39EE285 pKa = 4.2IGHH288 pKa = 6.23AVGLHH293 pKa = 6.24HH294 pKa = 7.29PGDD297 pKa = 4.36YY298 pKa = 10.35NASPDD303 pKa = 3.29IDD305 pKa = 3.24ITYY308 pKa = 10.3EE309 pKa = 3.56AFAQYY314 pKa = 10.66RR315 pKa = 11.84EE316 pKa = 4.26DD317 pKa = 3.73TQQYY321 pKa = 10.8SVMSYY326 pKa = 10.69FDD328 pKa = 3.7EE329 pKa = 4.8SNADD333 pKa = 3.19GSLGFGAFPDD343 pKa = 3.88TLMLHH348 pKa = 7.44DD349 pKa = 5.2ILALQQLYY357 pKa = 9.93GANTSTRR364 pKa = 11.84SGDD367 pKa = 3.08TTYY370 pKa = 11.36GFNSTEE376 pKa = 3.73GGVYY380 pKa = 10.38DD381 pKa = 5.25FSQAMPHH388 pKa = 7.27ALTLWDD394 pKa = 4.02AGGVDD399 pKa = 4.41TIDD402 pKa = 3.45LSGAGKK408 pKa = 10.09VDD410 pKa = 3.49QFVSLIDD417 pKa = 3.55GTYY420 pKa = 9.68SNVGGGLGNMAIAYY434 pKa = 8.57SAVIEE439 pKa = 4.07NAIGGRR445 pKa = 11.84GDD447 pKa = 4.7DD448 pKa = 3.58VVLGNAASNTLHH460 pKa = 6.75GKK462 pKa = 10.18AGDD465 pKa = 3.75DD466 pKa = 3.78TLVGGAGHH474 pKa = 7.45DD475 pKa = 3.67KK476 pKa = 11.2LFGGSGNDD484 pKa = 3.31RR485 pKa = 11.84LDD487 pKa = 3.88GGSSDD492 pKa = 5.02DD493 pKa = 4.02VLNGGAGDD501 pKa = 4.18DD502 pKa = 4.01SLIGGSGNDD511 pKa = 3.15RR512 pKa = 11.84LVGGSGNDD520 pKa = 3.46TYY522 pKa = 11.27TGGSGSDD529 pKa = 3.11MFCIYY534 pKa = 10.78NRR536 pKa = 11.84GDD538 pKa = 3.42AEE540 pKa = 4.69TITDD544 pKa = 4.12FQSGFDD550 pKa = 3.76TIAIRR555 pKa = 11.84SEE557 pKa = 3.78FAAFDD562 pKa = 3.8NLTILASGADD572 pKa = 3.79SVIKK576 pKa = 10.74FGDD579 pKa = 3.44NSISLLGVDD588 pKa = 5.19AGIISQNDD596 pKa = 3.45LAFVV600 pKa = 3.67

Molecular weight: 62.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562SLY9|A0A562SLY9_9RHOB Probable membrane transporter protein OS=Roseibium hamelinense OX=150831 GN=JM93_03579 PE=3 SV=1
MM1 pKa = 7.36GHH3 pKa = 7.46LFWLSDD9 pKa = 3.78EE10 pKa = 3.92QWAAIEE16 pKa = 4.11PLLPRR21 pKa = 11.84NKK23 pKa = 10.16GGARR27 pKa = 11.84RR28 pKa = 11.84VDD30 pKa = 3.5DD31 pKa = 3.8RR32 pKa = 11.84RR33 pKa = 11.84VISGIIHH40 pKa = 6.07GLKK43 pKa = 10.18VGCRR47 pKa = 11.84WCDD50 pKa = 3.36CPEE53 pKa = 4.02EE54 pKa = 4.37YY55 pKa = 10.87GPSTTIYY62 pKa = 10.63NRR64 pKa = 11.84FNRR67 pKa = 11.84WSRR70 pKa = 11.84KK71 pKa = 9.35RR72 pKa = 11.84FWIDD76 pKa = 3.16LVEE79 pKa = 4.26GLAICGAVTKK89 pKa = 9.64STSIDD94 pKa = 3.22STYY97 pKa = 11.31VKK99 pKa = 10.34AHH101 pKa = 6.76RR102 pKa = 11.84SAHH105 pKa = 5.41GGKK108 pKa = 10.02GGRR111 pKa = 11.84KK112 pKa = 7.96IRR114 pKa = 11.84LSGRR118 pKa = 11.84HH119 pKa = 4.87GAGKK123 pKa = 10.06RR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84STPLPTT132 pKa = 3.9

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4355

0

4355

1400321

21

2801

321.5

35.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.299 ± 0.046

0.889 ± 0.011

6.017 ± 0.041

6.003 ± 0.037

4.015 ± 0.024

8.272 ± 0.035

2.007 ± 0.02

5.387 ± 0.026

3.897 ± 0.028

10.007 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.018

3.055 ± 0.024

4.874 ± 0.03

3.395 ± 0.021

6.095 ± 0.037

5.958 ± 0.031

5.531 ± 0.03

7.26 ± 0.03

1.239 ± 0.014

2.322 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski