Amino acid dipepetide frequency for Bacillus anthracis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.08AlaAla: 5.08 ± 0.077	0.649AlaCys: 0.649 ± 0.021	2.897AlaAsp: 2.897 ± 0.051	4.345AlaGlu: 4.345 ± 0.068	3.295AlaPhe: 3.295 ± 0.055	4.804AlaGly: 4.804 ± 0.067	1.285AlaHis: 1.285 ± 0.033	5.856AlaIle: 5.856 ± 0.066	5.008AlaLys: 5.008 ± 0.07	6.922AlaLeu: 6.922 ± 0.07	2.02AlaMet: 2.02 ± 0.044	2.922AlaAsn: 2.922 ± 0.067	1.934AlaPro: 1.934 ± 0.042	2.035AlaGln: 2.035 ± 0.039	2.346AlaArg: 2.346 ± 0.05	3.795AlaSer: 3.795 ± 0.049	3.789AlaThr: 3.789 ± 0.07	5.01AlaVal: 5.01 ± 0.067	0.577AlaTrp: 0.577 ± 0.02	2.38AlaTyr: 2.38 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.45CysAla: 0.45 ± 0.017	0.12CysCys: 0.12 ± 0.01	0.425CysAsp: 0.425 ± 0.02	0.568CysGlu: 0.568 ± 0.023	0.446CysPhe: 0.446 ± 0.018	0.707CysGly: 0.707 ± 0.028	0.189CysHis: 0.189 ± 0.012	0.752CysIle: 0.752 ± 0.023	0.509CysLys: 0.509 ± 0.023	0.833CysLeu: 0.833 ± 0.027	0.269CysMet: 0.269 ± 0.014	0.386CysAsn: 0.386 ± 0.018	0.312CysPro: 0.312 ± 0.017	0.242CysGln: 0.242 ± 0.015	0.282CysArg: 0.282 ± 0.013	0.615CysSer: 0.615 ± 0.023	0.474CysThr: 0.474 ± 0.019	0.5CysVal: 0.5 ± 0.018	0.086CysTrp: 0.086 ± 0.009	0.313CysTyr: 0.313 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.076AspAla: 3.076 ± 0.046	0.404AspCys: 0.404 ± 0.019	2.138AspAsp: 2.138 ± 0.047	4.064AspGlu: 4.064 ± 0.053	2.193AspPhe: 2.193 ± 0.039	3.24AspGly: 3.24 ± 0.049	0.922AspHis: 0.922 ± 0.029	3.957AspIle: 3.957 ± 0.056	3.217AspLys: 3.217 ± 0.051	4.242AspLeu: 4.242 ± 0.056	1.247AspMet: 1.247 ± 0.027	1.763AspAsn: 1.763 ± 0.037	1.656AspPro: 1.656 ± 0.041	1.412AspGln: 1.412 ± 0.032	1.863AspArg: 1.863 ± 0.041	2.339AspSer: 2.339 ± 0.041	2.347AspThr: 2.347 ± 0.042	3.894AspVal: 3.894 ± 0.055	0.611AspTrp: 0.611 ± 0.021	1.997AspTyr: 1.997 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	5.04GluAla: 5.04 ± 0.073	0.5GluCys: 0.5 ± 0.019	3.534GluAsp: 3.534 ± 0.057	7.449GluGlu: 7.449 ± 0.115	2.582GluPhe: 2.582 ± 0.044	4.142GluGly: 4.142 ± 0.064	1.558GluHis: 1.558 ± 0.031	5.576GluIle: 5.576 ± 0.07	7.511GluLys: 7.511 ± 0.095	6.964GluLeu: 6.964 ± 0.074	2.379GluMet: 2.379 ± 0.043	3.826GluAsn: 3.826 ± 0.059	1.744GluPro: 1.744 ± 0.042	3.482GluGln: 3.482 ± 0.066	3.518GluArg: 3.518 ± 0.06	3.419GluSer: 3.419 ± 0.053	3.877GluThr: 3.877 ± 0.05	5.291GluVal: 5.291 ± 0.075	0.85GluTrp: 0.85 ± 0.024	2.439GluTyr: 2.439 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.111PheAla: 3.111 ± 0.053	0.444PheCys: 0.444 ± 0.016	2.104PheAsp: 2.104 ± 0.037	2.843PheGlu: 2.843 ± 0.043	2.48PhePhe: 2.48 ± 0.054	3.34PheGly: 3.34 ± 0.06	1.105PheHis: 1.105 ± 0.029	4.324PheIle: 4.324 ± 0.069	2.436PheLys: 2.436 ± 0.04	4.829PheLeu: 4.829 ± 0.066	1.243PheMet: 1.243 ± 0.034	1.789PheAsn: 1.789 ± 0.034	1.624PhePro: 1.624 ± 0.036	1.822PheGln: 1.822 ± 0.044	1.549PheArg: 1.549 ± 0.036	3.249PheSer: 3.249 ± 0.052	2.769PheThr: 2.769 ± 0.049	3.541PheVal: 3.541 ± 0.054	0.505PheTrp: 0.505 ± 0.022	1.788PheTyr: 1.788 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.729GlyAla: 4.729 ± 0.075	0.655GlyCys: 0.655 ± 0.026	3.056GlyAsp: 3.056 ± 0.057	4.429GlyGlu: 4.429 ± 0.059	3.303GlyPhe: 3.303 ± 0.05	4.608GlyGly: 4.608 ± 0.072	1.33GlyHis: 1.33 ± 0.038	5.969GlyIle: 5.969 ± 0.078	5.136GlyLys: 5.136 ± 0.069	6.147GlyLeu: 6.147 ± 0.098	2.069GlyMet: 2.069 ± 0.042	2.919GlyAsn: 2.919 ± 0.058	1.636GlyPro: 1.636 ± 0.061	1.993GlyGln: 1.993 ± 0.038	2.314GlyArg: 2.314 ± 0.049	3.719GlySer: 3.719 ± 0.06	4.033GlyThr: 4.033 ± 0.063	4.972GlyVal: 4.972 ± 0.059	0.8GlyTrp: 0.8 ± 0.026	2.728GlyTyr: 2.728 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.322HisAla: 1.322 ± 0.029	0.206HisCys: 0.206 ± 0.012	1.044HisAsp: 1.044 ± 0.03	1.437HisGlu: 1.437 ± 0.031	1.082HisPhe: 1.082 ± 0.031	1.316HisGly: 1.316 ± 0.033	0.68HisHis: 0.68 ± 0.031	1.866HisIle: 1.866 ± 0.037	1.189HisLys: 1.189 ± 0.031	1.966HisLeu: 1.966 ± 0.038	0.595HisMet: 0.595 ± 0.021	0.951HisAsn: 0.951 ± 0.03	1.0HisPro: 1.0 ± 0.029	0.652HisGln: 0.652 ± 0.023	0.796HisArg: 0.796 ± 0.023	1.23HisSer: 1.23 ± 0.03	1.198HisThr: 1.198 ± 0.029	1.556HisVal: 1.556 ± 0.032	0.22HisTrp: 0.22 ± 0.014	0.916HisTyr: 0.916 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.197IleAla: 6.197 ± 0.075	0.882IleCys: 0.882 ± 0.023	3.993IleAsp: 3.993 ± 0.057	5.874IleGlu: 5.874 ± 0.071	3.646IlePhe: 3.646 ± 0.064	6.28IleGly: 6.28 ± 0.082	1.82IleHis: 1.82 ± 0.033	6.29IleIle: 6.29 ± 0.084	4.515IleLys: 4.515 ± 0.063	7.267IleLeu: 7.267 ± 0.085	2.033IleMet: 2.033 ± 0.042	3.146IleAsn: 3.146 ± 0.072	3.427IlePro: 3.427 ± 0.067	3.017IleGln: 3.017 ± 0.053	2.972IleArg: 2.972 ± 0.045	5.175IleSer: 5.175 ± 0.066	4.763IleThr: 4.763 ± 0.071	6.219IleVal: 6.219 ± 0.078	0.685IleTrp: 0.685 ± 0.023	2.526IleTyr: 2.526 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.503LysAla: 4.503 ± 0.065	0.399LysCys: 0.399 ± 0.018	4.077LysAsp: 4.077 ± 0.062	8.039LysGlu: 8.039 ± 0.102	2.214LysPhe: 2.214 ± 0.036	4.603LysGly: 4.603 ± 0.059	1.419LysHis: 1.419 ± 0.027	4.807LysIle: 4.807 ± 0.064	6.728LysLys: 6.728 ± 0.097	6.065LysLeu: 6.065 ± 0.081	2.567LysMet: 2.567 ± 0.044	3.709LysAsn: 3.709 ± 0.053	2.193LysPro: 2.193 ± 0.046	3.722LysGln: 3.722 ± 0.059	3.576LysArg: 3.576 ± 0.06	3.573LysSer: 3.573 ± 0.051	3.743LysThr: 3.743 ± 0.05	5.179LysVal: 5.179 ± 0.069	0.92LysTrp: 0.92 ± 0.024	2.614LysTyr: 2.614 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	6.649LeuAla: 6.649 ± 0.084	0.884LeuCys: 0.884 ± 0.026	4.129LeuAsp: 4.129 ± 0.055	6.409LeuGlu: 6.409 ± 0.069	5.054LeuPhe: 5.054 ± 0.085	6.105LeuGly: 6.105 ± 0.07	2.24LeuHis: 2.24 ± 0.046	6.983LeuIle: 6.983 ± 0.092	6.478LeuLys: 6.478 ± 0.069	10.106LeuLeu: 10.106 ± 0.116	2.348LeuMet: 2.348 ± 0.042	4.19LeuAsn: 4.19 ± 0.065	3.727LeuPro: 3.727 ± 0.056	4.376LeuGln: 4.376 ± 0.056	3.373LeuArg: 3.373 ± 0.049	6.341LeuSer: 6.341 ± 0.062	5.214LeuThr: 5.214 ± 0.063	6.136LeuVal: 6.136 ± 0.075	0.79LeuTrp: 0.79 ± 0.023	3.517LeuTyr: 3.517 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.768MetAla: 1.768 ± 0.037	0.192MetCys: 0.192 ± 0.011	1.314MetAsp: 1.314 ± 0.028	1.993MetGlu: 1.993 ± 0.038	1.244MetPhe: 1.244 ± 0.033	1.693MetGly: 1.693 ± 0.043	0.5MetHis: 0.5 ± 0.019	2.208MetIle: 2.208 ± 0.042	3.042MetLys: 3.042 ± 0.047	2.728MetLeu: 2.728 ± 0.043	0.931MetMet: 0.931 ± 0.027	1.711MetAsn: 1.711 ± 0.036	0.975MetPro: 0.975 ± 0.027	1.093MetGln: 1.093 ± 0.025	1.133MetArg: 1.133 ± 0.03	1.645MetSer: 1.645 ± 0.028	1.567MetThr: 1.567 ± 0.031	1.694MetVal: 1.694 ± 0.035	0.22MetTrp: 0.22 ± 0.013	0.996MetTyr: 0.996 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.746AsnAla: 2.746 ± 0.047	0.365AsnCys: 0.365 ± 0.017	2.205AsnAsp: 2.205 ± 0.04	3.909AsnGlu: 3.909 ± 0.058	1.77AsnPhe: 1.77 ± 0.032	3.407AsnGly: 3.407 ± 0.064	1.037AsnHis: 1.037 ± 0.029	3.87AsnIle: 3.87 ± 0.068	3.507AsnLys: 3.507 ± 0.055	3.646AsnLeu: 3.646 ± 0.047	1.347AsnMet: 1.347 ± 0.031	2.199AsnAsn: 2.199 ± 0.051	2.123AsnPro: 2.123 ± 0.061	1.684AsnGln: 1.684 ± 0.036	1.956AsnArg: 1.956 ± 0.041	2.346AsnSer: 2.346 ± 0.06	2.453AsnThr: 2.453 ± 0.069	3.475AsnVal: 3.475 ± 0.055	0.533AsnTrp: 0.533 ± 0.02	1.631AsnTyr: 1.631 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	2.143ProAla: 2.143 ± 0.047	0.219ProCys: 0.219 ± 0.013	1.579ProAsp: 1.579 ± 0.04	2.504ProGlu: 2.504 ± 0.043	1.958ProPhe: 1.958 ± 0.04	1.952ProGly: 1.952 ± 0.042	0.773ProHis: 0.773 ± 0.023	2.892ProIle: 2.892 ± 0.059	2.323ProLys: 2.323 ± 0.045	3.304ProLeu: 3.304 ± 0.056	0.807ProMet: 0.807 ± 0.025	1.86ProAsn: 1.86 ± 0.063	0.894ProPro: 0.894 ± 0.033	1.01ProGln: 1.01 ± 0.031	0.962ProArg: 0.962 ± 0.028	2.161ProSer: 2.161 ± 0.043	1.975ProThr: 1.975 ± 0.063	2.687ProVal: 2.687 ± 0.049	0.345ProTrp: 0.345 ± 0.017	1.441ProTyr: 1.441 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.38GlnAla: 2.38 ± 0.046	0.227GlnCys: 0.227 ± 0.011	1.55GlnAsp: 1.55 ± 0.03	2.858GlnGlu: 2.858 ± 0.052	1.656GlnPhe: 1.656 ± 0.035	2.186GlnGly: 2.186 ± 0.04	0.854GlnHis: 0.854 ± 0.026	2.704GlnIle: 2.704 ± 0.045	3.114GlnLys: 3.114 ± 0.047	3.821GlnLeu: 3.821 ± 0.06	1.105GlnMet: 1.105 ± 0.029	1.893GlnAsn: 1.893 ± 0.04	1.116GlnPro: 1.116 ± 0.033	1.735GlnGln: 1.735 ± 0.046	1.344GlnArg: 1.344 ± 0.033	2.09GlnSer: 2.09 ± 0.044	2.032GlnThr: 2.032 ± 0.038	2.491GlnVal: 2.491 ± 0.053	0.347GlnTrp: 0.347 ± 0.018	1.503GlnTyr: 1.503 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.328ArgAla: 2.328 ± 0.044	0.256ArgCys: 0.256 ± 0.013	1.843ArgAsp: 1.843 ± 0.038	3.123ArgGlu: 3.123 ± 0.048	1.786ArgPhe: 1.786 ± 0.037	2.111ArgGly: 2.111 ± 0.047	0.736ArgHis: 0.736 ± 0.025	3.001ArgIle: 3.001 ± 0.05	3.329ArgLys: 3.329 ± 0.056	3.565ArgLeu: 3.565 ± 0.051	1.227ArgMet: 1.227 ± 0.032	1.943ArgAsn: 1.943 ± 0.035	1.138ArgPro: 1.138 ± 0.032	1.331ArgGln: 1.331 ± 0.028	1.557ArgArg: 1.557 ± 0.037	1.919ArgSer: 1.919 ± 0.035	1.984ArgThr: 1.984 ± 0.038	2.554ArgVal: 2.554 ± 0.043	0.401ArgTrp: 0.401 ± 0.016	1.509ArgTyr: 1.509 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	3.603SerAla: 3.603 ± 0.057	0.493SerCys: 0.493 ± 0.024	2.394SerAsp: 2.394 ± 0.048	3.489SerGlu: 3.489 ± 0.055	3.458SerPhe: 3.458 ± 0.05	3.905SerGly: 3.905 ± 0.062	1.208SerHis: 1.208 ± 0.032	5.283SerIle: 5.283 ± 0.079	4.009SerLys: 4.009 ± 0.061	5.95SerLeu: 5.95 ± 0.073	1.713SerMet: 1.713 ± 0.033	2.717SerAsn: 2.717 ± 0.057	1.913SerPro: 1.913 ± 0.046	1.737SerGln: 1.737 ± 0.038	1.956SerArg: 1.956 ± 0.034	3.769SerSer: 3.769 ± 0.055	3.152SerThr: 3.152 ± 0.058	4.101SerVal: 4.101 ± 0.058	0.611SerTrp: 0.611 ± 0.019	2.48SerTyr: 2.48 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	3.725ThrAla: 3.725 ± 0.064	0.481ThrCys: 0.481 ± 0.018	2.403ThrAsp: 2.403 ± 0.044	3.691ThrGlu: 3.691 ± 0.046	2.95ThrPhe: 2.95 ± 0.061	4.21ThrGly: 4.21 ± 0.127	1.088ThrHis: 1.088 ± 0.026	4.857ThrIle: 4.857 ± 0.086	4.002ThrLys: 4.002 ± 0.052	5.432ThrLeu: 5.432 ± 0.059	1.396ThrMet: 1.396 ± 0.032	2.712ThrAsn: 2.712 ± 0.054	2.158ThrPro: 2.158 ± 0.04	1.376ThrGln: 1.376 ± 0.03	1.683ThrArg: 1.683 ± 0.034	3.21ThrSer: 3.21 ± 0.06	3.165ThrThr: 3.165 ± 0.087	4.397ThrVal: 4.397 ± 0.079	0.53ThrTrp: 0.53 ± 0.021	2.271ThrTyr: 2.271 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.246ValAla: 5.246 ± 0.068	0.664ValCys: 0.664 ± 0.019	3.359ValAsp: 3.359 ± 0.049	5.006ValGlu: 5.006 ± 0.075	3.313ValPhe: 3.313 ± 0.051	4.702ValGly: 4.702 ± 0.065	1.447ValHis: 1.447 ± 0.035	5.708ValIle: 5.708 ± 0.063	5.146ValLys: 5.146 ± 0.076	7.014ValLeu: 7.014 ± 0.065	1.987ValMet: 1.987 ± 0.032	3.203ValAsn: 3.203 ± 0.052	2.682ValPro: 2.682 ± 0.047	2.747ValGln: 2.747 ± 0.052	2.598ValArg: 2.598 ± 0.045	4.568ValSer: 4.568 ± 0.059	4.581ValThr: 4.581 ± 0.104	5.433ValVal: 5.433 ± 0.069	0.691ValTrp: 0.691 ± 0.025	2.425ValTyr: 2.425 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.554TrpAla: 0.554 ± 0.019	0.088TrpCys: 0.088 ± 0.007	0.563TrpAsp: 0.563 ± 0.019	0.686TrpGlu: 0.686 ± 0.025	0.529TrpPhe: 0.529 ± 0.02	0.699TrpGly: 0.699 ± 0.023	0.215TrpHis: 0.215 ± 0.013	0.947TrpIle: 0.947 ± 0.033	0.813TrpLys: 0.813 ± 0.021	1.062TrpLeu: 1.062 ± 0.029	0.311TrpMet: 0.311 ± 0.016	0.642TrpAsn: 0.642 ± 0.025	0.205TrpPro: 0.205 ± 0.012	0.338TrpGln: 0.338 ± 0.015	0.374TrpArg: 0.374 ± 0.015	0.613TrpSer: 0.613 ± 0.021	0.494TrpThr: 0.494 ± 0.018	0.611TrpVal: 0.611 ± 0.018	0.14TrpTrp: 0.14 ± 0.012	0.372TrpTyr: 0.372 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.298TyrAla: 2.298 ± 0.042	0.347TyrCys: 0.347 ± 0.016	1.973TyrAsp: 1.973 ± 0.045	2.965TyrGlu: 2.965 ± 0.045	1.937TyrPhe: 1.937 ± 0.038	2.531TyrGly: 2.531 ± 0.041	0.817TyrHis: 0.817 ± 0.023	2.876TyrIle: 2.876 ± 0.045	2.638TyrLys: 2.638 ± 0.045	3.187TyrLeu: 3.187 ± 0.046	1.053TyrMet: 1.053 ± 0.026	1.727TyrAsn: 1.727 ± 0.034	1.334TyrPro: 1.334 ± 0.03	1.203TyrGln: 1.203 ± 0.032	1.49TyrArg: 1.49 ± 0.03	2.166TyrSer: 2.166 ± 0.039	2.127TyrThr: 2.127 ± 0.04	2.782TyrVal: 2.782 ± 0.043	0.393TyrTrp: 0.393 ± 0.016	1.624TyrTyr: 1.624 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5493 proteins (1439306 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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