Amino acid dipepetide frequency for Clostridium disporicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.901AlaAla: 3.901 ± 0.088	0.702AlaCys: 0.702 ± 0.029	2.659AlaAsp: 2.659 ± 0.056	3.511AlaGlu: 3.511 ± 0.067	2.462AlaPhe: 2.462 ± 0.058	3.85AlaGly: 3.85 ± 0.077	0.804AlaHis: 0.804 ± 0.03	6.06AlaIle: 6.06 ± 0.09	4.613AlaLys: 4.613 ± 0.069	5.843AlaLeu: 5.843 ± 0.088	1.757AlaMet: 1.757 ± 0.042	2.82AlaAsn: 2.82 ± 0.063	1.438AlaPro: 1.438 ± 0.039	1.322AlaGln: 1.322 ± 0.032	1.86AlaArg: 1.86 ± 0.049	3.082AlaSer: 3.082 ± 0.053	3.135AlaThr: 3.135 ± 0.059	4.16AlaVal: 4.16 ± 0.071	0.395AlaTrp: 0.395 ± 0.023	2.221AlaTyr: 2.221 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.599CysAla: 0.599 ± 0.027	0.227CysCys: 0.227 ± 0.019	0.752CysAsp: 0.752 ± 0.026	0.806CysGlu: 0.806 ± 0.029	0.472CysPhe: 0.472 ± 0.024	1.155CysGly: 1.155 ± 0.042	0.203CysHis: 0.203 ± 0.014	1.186CysIle: 1.186 ± 0.036	1.015CysLys: 1.015 ± 0.034	0.837CysLeu: 0.837 ± 0.03	0.286CysMet: 0.286 ± 0.018	0.8CysAsn: 0.8 ± 0.029	0.478CysPro: 0.478 ± 0.025	0.217CysGln: 0.217 ± 0.016	0.381CysArg: 0.381 ± 0.02	0.714CysSer: 0.714 ± 0.026	0.635CysThr: 0.635 ± 0.027	0.619CysVal: 0.619 ± 0.028	0.069CysTrp: 0.069 ± 0.009	0.464CysTyr: 0.464 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	2.634AspAla: 2.634 ± 0.057	0.585AspCys: 0.585 ± 0.024	3.087AspAsp: 3.087 ± 0.066	5.01AspGlu: 5.01 ± 0.079	2.718AspPhe: 2.718 ± 0.051	3.698AspGly: 3.698 ± 0.068	0.556AspHis: 0.556 ± 0.023	6.342AspIle: 6.342 ± 0.097	5.55AspLys: 5.55 ± 0.075	5.03AspLeu: 5.03 ± 0.07	1.567AspMet: 1.567 ± 0.041	3.638AspAsn: 3.638 ± 0.08	1.217AspPro: 1.217 ± 0.035	0.775AspGln: 0.775 ± 0.031	1.971AspArg: 1.971 ± 0.047	3.355AspSer: 3.355 ± 0.059	2.619AspThr: 2.619 ± 0.06	3.722AspVal: 3.722 ± 0.065	0.343AspTrp: 0.343 ± 0.022	2.824AspTyr: 2.824 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	4.332GluAla: 4.332 ± 0.084	0.746GluCys: 0.746 ± 0.031	4.544GluAsp: 4.544 ± 0.073	7.59GluGlu: 7.59 ± 0.115	3.135GluPhe: 3.135 ± 0.065	4.443GluGly: 4.443 ± 0.076	0.855GluHis: 0.855 ± 0.027	7.272GluIle: 7.272 ± 0.111	7.192GluLys: 7.192 ± 0.098	6.99GluLeu: 6.99 ± 0.098	1.956GluMet: 1.956 ± 0.047	5.364GluAsn: 5.364 ± 0.076	1.404GluPro: 1.404 ± 0.045	1.591GluGln: 1.591 ± 0.047	2.696GluArg: 2.696 ± 0.046	3.794GluSer: 3.794 ± 0.064	2.938GluThr: 2.938 ± 0.057	5.59GluVal: 5.59 ± 0.078	0.45GluTrp: 0.45 ± 0.021	3.242GluTyr: 3.242 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	2.353PheAla: 2.353 ± 0.056	0.499PheCys: 0.499 ± 0.025	2.616PheAsp: 2.616 ± 0.051	2.691PheGlu: 2.691 ± 0.055	1.9PhePhe: 1.9 ± 0.05	3.019PheGly: 3.019 ± 0.056	0.471PheHis: 0.471 ± 0.023	4.996PheIle: 4.996 ± 0.093	3.675PheLys: 3.675 ± 0.064	3.894PheLeu: 3.894 ± 0.077	1.184PheMet: 1.184 ± 0.037	3.085PheAsn: 3.085 ± 0.063	1.124PhePro: 1.124 ± 0.037	0.845PheGln: 0.845 ± 0.032	1.324PheArg: 1.324 ± 0.035	2.919PheSer: 2.919 ± 0.061	2.401PheThr: 2.401 ± 0.051	2.763PheVal: 2.763 ± 0.053	0.287PheTrp: 0.287 ± 0.017	1.812PheTyr: 1.812 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	4.2GlyAla: 4.2 ± 0.08	0.978GlyCys: 0.978 ± 0.039	3.357GlyAsp: 3.357 ± 0.066	4.61GlyGlu: 4.61 ± 0.077	3.101GlyPhe: 3.101 ± 0.052	4.544GlyGly: 4.544 ± 0.082	0.958GlyHis: 0.958 ± 0.03	6.868GlyIle: 6.868 ± 0.095	5.325GlyLys: 5.325 ± 0.081	5.755GlyLeu: 5.755 ± 0.095	1.755GlyMet: 1.755 ± 0.05	3.405GlyAsn: 3.405 ± 0.068	1.247GlyPro: 1.247 ± 0.043	1.453GlyGln: 1.453 ± 0.043	2.427GlyArg: 2.427 ± 0.056	3.611GlySer: 3.611 ± 0.058	3.624GlyThr: 3.624 ± 0.068	4.939GlyVal: 4.939 ± 0.068	0.515GlyTrp: 0.515 ± 0.026	3.073GlyTyr: 3.073 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	0.59HisAla: 0.59 ± 0.023	0.203HisCys: 0.203 ± 0.015	0.663HisAsp: 0.663 ± 0.025	0.768HisGlu: 0.768 ± 0.026	0.577HisPhe: 0.577 ± 0.025	1.003HisGly: 1.003 ± 0.034	0.285HisHis: 0.285 ± 0.019	1.201HisIle: 1.201 ± 0.041	1.048HisLys: 1.048 ± 0.036	1.083HisLeu: 1.083 ± 0.036	0.358HisMet: 0.358 ± 0.019	0.805HisAsn: 0.805 ± 0.029	0.58HisPro: 0.58 ± 0.024	0.294HisGln: 0.294 ± 0.019	0.472HisArg: 0.472 ± 0.022	0.795HisSer: 0.795 ± 0.028	0.588HisThr: 0.588 ± 0.023	0.701HisVal: 0.701 ± 0.028	0.106HisTrp: 0.106 ± 0.011	0.624HisTyr: 0.624 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.177IleAla: 6.177 ± 0.097	1.251IleCys: 1.251 ± 0.037	6.231IleAsp: 6.231 ± 0.088	7.312IleGlu: 7.312 ± 0.097	4.449IlePhe: 4.449 ± 0.086	6.357IleGly: 6.357 ± 0.099	1.209IleHis: 1.209 ± 0.043	9.753IleIle: 9.753 ± 0.126	8.612IleLys: 8.612 ± 0.106	9.058IleLeu: 9.058 ± 0.128	2.351IleMet: 2.351 ± 0.05	6.759IleAsn: 6.759 ± 0.091	3.149IlePro: 3.149 ± 0.056	2.072IleGln: 2.072 ± 0.045	3.089IleArg: 3.089 ± 0.06	6.846IleSer: 6.846 ± 0.084	5.037IleThr: 5.037 ± 0.076	6.467IleVal: 6.467 ± 0.093	0.568IleTrp: 0.568 ± 0.025	3.875IleTyr: 3.875 ± 0.073	0.0IleXaa: 0.0 ± 0.0
Lys	4.715LysAla: 4.715 ± 0.07	0.948LysCys: 0.948 ± 0.034	5.71LysAsp: 5.71 ± 0.086	8.632LysGlu: 8.632 ± 0.118	3.253LysPhe: 3.253 ± 0.05	4.88LysGly: 4.88 ± 0.077	0.987LysHis: 0.987 ± 0.033	7.753LysIle: 7.753 ± 0.086	7.463LysLys: 7.463 ± 0.092	7.1LysLeu: 7.1 ± 0.087	2.303LysMet: 2.303 ± 0.052	5.858LysAsn: 5.858 ± 0.078	1.953LysPro: 1.953 ± 0.044	1.729LysGln: 1.729 ± 0.044	3.085LysArg: 3.085 ± 0.059	5.02LysSer: 5.02 ± 0.081	3.672LysThr: 3.672 ± 0.066	6.089LysVal: 6.089 ± 0.074	0.592LysTrp: 0.592 ± 0.025	4.13LysTyr: 4.13 ± 0.073	0.0LysXaa: 0.0 ± 0.0
Leu	5.424LeuAla: 5.424 ± 0.088	1.057LeuCys: 1.057 ± 0.032	5.346LeuAsp: 5.346 ± 0.078	6.597LeuGlu: 6.597 ± 0.094	3.697LeuPhe: 3.697 ± 0.072	6.193LeuGly: 6.193 ± 0.088	1.075LeuHis: 1.075 ± 0.036	8.469LeuIle: 8.469 ± 0.115	7.841LeuLys: 7.841 ± 0.092	7.893LeuLeu: 7.893 ± 0.119	2.29LeuMet: 2.29 ± 0.049	5.77LeuAsn: 5.77 ± 0.082	2.727LeuPro: 2.727 ± 0.06	1.93LeuGln: 1.93 ± 0.048	3.093LeuArg: 3.093 ± 0.055	6.498LeuSer: 6.498 ± 0.078	4.677LeuThr: 4.677 ± 0.062	5.623LeuVal: 5.623 ± 0.076	0.61LeuTrp: 0.61 ± 0.029	3.275LeuTyr: 3.275 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.822MetAla: 1.822 ± 0.054	0.26MetCys: 0.26 ± 0.018	1.494MetAsp: 1.494 ± 0.039	1.732MetGlu: 1.732 ± 0.04	1.002MetPhe: 1.002 ± 0.035	1.729MetGly: 1.729 ± 0.049	0.331MetHis: 0.331 ± 0.021	2.42MetIle: 2.42 ± 0.052	2.443MetLys: 2.443 ± 0.048	2.238MetLeu: 2.238 ± 0.048	0.745MetMet: 0.745 ± 0.027	1.712MetAsn: 1.712 ± 0.04	0.913MetPro: 0.913 ± 0.032	0.611MetGln: 0.611 ± 0.026	0.917MetArg: 0.917 ± 0.03	1.645MetSer: 1.645 ± 0.044	1.236MetThr: 1.236 ± 0.035	1.662MetVal: 1.662 ± 0.046	0.188MetTrp: 0.188 ± 0.015	0.907MetTyr: 0.907 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.785AsnAla: 2.785 ± 0.063	0.762AsnCys: 0.762 ± 0.03	3.54AsnAsp: 3.54 ± 0.062	4.531AsnGlu: 4.531 ± 0.071	2.696AsnPhe: 2.696 ± 0.063	4.039AsnGly: 4.039 ± 0.064	0.842AsnHis: 0.842 ± 0.029	7.418AsnIle: 7.418 ± 0.092	5.907AsnLys: 5.907 ± 0.081	5.524AsnLeu: 5.524 ± 0.089	1.581AsnMet: 1.581 ± 0.038	4.86AsnAsn: 4.86 ± 0.102	2.111AsnPro: 2.111 ± 0.046	1.207AsnGln: 1.207 ± 0.039	2.107AsnArg: 2.107 ± 0.049	3.987AsnSer: 3.987 ± 0.07	2.945AsnThr: 2.945 ± 0.063	3.598AsnVal: 3.598 ± 0.065	0.432AsnTrp: 0.432 ± 0.024	2.973AsnTyr: 2.973 ± 0.062	0.0AsnXaa: 0.0 ± 0.0
Pro	1.409ProAla: 1.409 ± 0.045	0.313ProCys: 0.313 ± 0.019	1.329ProAsp: 1.329 ± 0.04	2.174ProGlu: 2.174 ± 0.051	1.356ProPhe: 1.356 ± 0.041	1.699ProGly: 1.699 ± 0.045	0.431ProHis: 0.431 ± 0.022	2.67ProIle: 2.67 ± 0.057	1.957ProLys: 1.957 ± 0.048	2.444ProLeu: 2.444 ± 0.043	0.73ProMet: 0.73 ± 0.028	1.585ProAsn: 1.585 ± 0.043	0.557ProPro: 0.557 ± 0.025	0.742ProGln: 0.742 ± 0.031	0.829ProArg: 0.829 ± 0.029	1.772ProSer: 1.772 ± 0.047	1.552ProThr: 1.552 ± 0.042	2.017ProVal: 2.017 ± 0.046	0.256ProTrp: 0.256 ± 0.019	1.289ProTyr: 1.289 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.268GlnAla: 1.268 ± 0.036	0.227GlnCys: 0.227 ± 0.015	1.127GlnAsp: 1.127 ± 0.035	1.574GlnGlu: 1.574 ± 0.042	0.915GlnPhe: 0.915 ± 0.034	1.501GlnGly: 1.501 ± 0.047	0.256GlnHis: 0.256 ± 0.016	1.868GlnIle: 1.868 ± 0.047	1.688GlnLys: 1.688 ± 0.043	1.828GlnLeu: 1.828 ± 0.048	0.596GlnMet: 0.596 ± 0.026	1.26GlnAsn: 1.26 ± 0.031	0.498GlnPro: 0.498 ± 0.023	0.51GlnGln: 0.51 ± 0.025	0.899GlnArg: 0.899 ± 0.032	1.166GlnSer: 1.166 ± 0.034	0.918GlnThr: 0.918 ± 0.033	1.463GlnVal: 1.463 ± 0.039	0.181GlnTrp: 0.181 ± 0.013	1.06GlnTyr: 1.06 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	1.848ArgAla: 1.848 ± 0.046	0.459ArgCys: 0.459 ± 0.023	1.992ArgAsp: 1.992 ± 0.05	3.082ArgGlu: 3.082 ± 0.061	1.521ArgPhe: 1.521 ± 0.04	2.139ArgGly: 2.139 ± 0.049	0.42ArgHis: 0.42 ± 0.02	3.185ArgIle: 3.185 ± 0.063	2.915ArgLys: 2.915 ± 0.057	2.953ArgLeu: 2.953 ± 0.055	0.903ArgMet: 0.903 ± 0.031	2.006ArgAsn: 2.006 ± 0.045	0.856ArgPro: 0.856 ± 0.03	0.785ArgGln: 0.785 ± 0.028	1.489ArgArg: 1.489 ± 0.047	1.555ArgSer: 1.555 ± 0.047	1.569ArgThr: 1.569 ± 0.044	2.441ArgVal: 2.441 ± 0.051	0.256ArgTrp: 0.256 ± 0.017	1.584ArgTyr: 1.584 ± 0.041	0.001ArgXaa: 0.001 ± 0.001
Ser	3.016SerAla: 3.016 ± 0.062	0.648SerCys: 0.648 ± 0.027	3.211SerAsp: 3.211 ± 0.06	4.071SerGlu: 4.071 ± 0.066	3.016SerPhe: 3.016 ± 0.062	4.231SerGly: 4.231 ± 0.07	0.862SerHis: 0.862 ± 0.032	6.657SerIle: 6.657 ± 0.11	5.506SerLys: 5.506 ± 0.096	5.995SerLeu: 5.995 ± 0.084	1.553SerMet: 1.553 ± 0.043	3.984SerAsn: 3.984 ± 0.075	1.575SerPro: 1.575 ± 0.045	1.384SerGln: 1.384 ± 0.046	2.017SerArg: 2.017 ± 0.048	4.007SerSer: 4.007 ± 0.076	3.211SerThr: 3.211 ± 0.059	3.626SerVal: 3.626 ± 0.059	0.423SerTrp: 0.423 ± 0.022	2.623SerTyr: 2.623 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	2.929ThrAla: 2.929 ± 0.069	0.53ThrCys: 0.53 ± 0.022	2.49ThrAsp: 2.49 ± 0.056	3.127ThrGlu: 3.127 ± 0.064	2.374ThrPhe: 2.374 ± 0.053	3.567ThrGly: 3.567 ± 0.07	0.708ThrHis: 0.708 ± 0.028	5.027ThrIle: 5.027 ± 0.088	3.498ThrLys: 3.498 ± 0.06	5.037ThrLeu: 5.037 ± 0.072	1.221ThrMet: 1.221 ± 0.035	2.759ThrAsn: 2.759 ± 0.062	1.848ThrPro: 1.848 ± 0.047	0.985ThrGln: 0.985 ± 0.029	1.535ThrArg: 1.535 ± 0.039	3.184ThrSer: 3.184 ± 0.059	2.808ThrThr: 2.808 ± 0.059	3.611ThrVal: 3.611 ± 0.072	0.335ThrTrp: 0.335 ± 0.018	1.986ThrTyr: 1.986 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.218ValAla: 4.218 ± 0.076	0.855ValCys: 0.855 ± 0.033	4.069ValAsp: 4.069 ± 0.064	4.783ValGlu: 4.783 ± 0.071	2.945ValPhe: 2.945 ± 0.058	4.429ValGly: 4.429 ± 0.076	0.817ValHis: 0.817 ± 0.026	6.493ValIle: 6.493 ± 0.077	5.375ValLys: 5.375 ± 0.082	6.18ValLeu: 6.18 ± 0.078	1.646ValMet: 1.646 ± 0.035	3.909ValAsn: 3.909 ± 0.059	1.945ValPro: 1.945 ± 0.046	1.334ValGln: 1.334 ± 0.033	2.029ValArg: 2.029 ± 0.044	4.336ValSer: 4.336 ± 0.063	3.574ValThr: 3.574 ± 0.061	4.928ValVal: 4.928 ± 0.077	0.408ValTrp: 0.408 ± 0.019	2.491ValTyr: 2.491 ± 0.046	0.001ValXaa: 0.001 ± 0.001
Trp	0.377TrpAla: 0.377 ± 0.02	0.111TrpCys: 0.111 ± 0.011	0.372TrpAsp: 0.372 ± 0.019	0.41TrpGlu: 0.41 ± 0.023	0.329TrpPhe: 0.329 ± 0.021	0.523TrpGly: 0.523 ± 0.028	0.11TrpHis: 0.11 ± 0.011	0.755TrpIle: 0.755 ± 0.034	0.454TrpLys: 0.454 ± 0.025	0.564TrpLeu: 0.564 ± 0.025	0.198TrpMet: 0.198 ± 0.015	0.441TrpAsn: 0.441 ± 0.022	0.176TrpPro: 0.176 ± 0.013	0.221TrpGln: 0.221 ± 0.016	0.249TrpArg: 0.249 ± 0.017	0.409TrpSer: 0.409 ± 0.023	0.322TrpThr: 0.322 ± 0.019	0.398TrpVal: 0.398 ± 0.019	0.091TrpTrp: 0.091 ± 0.01	0.272TrpTyr: 0.272 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.995TyrAla: 1.995 ± 0.047	0.553TyrCys: 0.553 ± 0.025	2.652TyrAsp: 2.652 ± 0.051	3.113TyrGlu: 3.113 ± 0.068	1.94TyrPhe: 1.94 ± 0.044	2.744TyrGly: 2.744 ± 0.051	0.563TyrHis: 0.563 ± 0.024	4.229TyrIle: 4.229 ± 0.084	3.8TyrLys: 3.8 ± 0.069	3.84TyrLeu: 3.84 ± 0.061	1.028TyrMet: 1.028 ± 0.032	3.025TyrAsn: 3.025 ± 0.068	1.314TyrPro: 1.314 ± 0.036	0.796TyrGln: 0.796 ± 0.031	1.491TyrArg: 1.491 ± 0.037	2.922TyrSer: 2.922 ± 0.054	2.095TyrThr: 2.095 ± 0.052	2.347TyrVal: 2.347 ± 0.051	0.275TyrTrp: 0.275 ± 0.017	2.016TyrTyr: 2.016 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.012XaaXaa: 0.012 ± 0.014

Statistics based on 3202 proteins (981246 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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