Neolecta irregularis (strain DAH-3)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Neolectomycetes; Neolectales; Neolectaceae; Neolecta; Neolecta irregularis

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7LT80|A0A1U7LT80_NEOID Cys-Gly metallodipeptidase dug1 OS=Neolecta irregularis (strain DAH-3) OX=1198029 GN=NEOLI_002715 PE=3 SV=1
MM1 pKa = 7.23NLGHH5 pKa = 6.2GVEE8 pKa = 4.76GRR10 pKa = 11.84AEE12 pKa = 3.84AAVDD16 pKa = 4.04AEE18 pKa = 4.55YY19 pKa = 10.73LVCDD23 pKa = 4.63DD24 pKa = 5.0GGDD27 pKa = 3.78GEE29 pKa = 4.75AVEE32 pKa = 5.43GVDD35 pKa = 4.67EE36 pKa = 5.08GFPCFDD42 pKa = 3.59ARR44 pKa = 11.84AALALVVEE52 pKa = 5.84AIHH55 pKa = 6.92AGDD58 pKa = 3.04VCAFVVSAA66 pKa = 4.91

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7LH51|A0A1U7LH51_NEOID DNA-directed RNA polymerases I II and III subunit RPABC2 OS=Neolecta irregularis (strain DAH-3) OX=1198029 GN=NEOLI_001797 PE=3 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84MSMRR6 pKa = 11.84QPASSRR12 pKa = 11.84SPSRR16 pKa = 11.84SRR18 pKa = 11.84SPPISAPLPLPLSSPSARR36 pKa = 11.84TPSANRR42 pKa = 11.84RR43 pKa = 11.84SMPSTMRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84PQSISSIQHH61 pKa = 5.53NAPPPLSSRR70 pKa = 11.84ARR72 pKa = 11.84KK73 pKa = 6.28TQSVADD79 pKa = 3.96LQHH82 pKa = 6.39PKK84 pKa = 10.39KK85 pKa = 10.49KK86 pKa = 10.29RR87 pKa = 11.84FPALRR92 pKa = 11.84KK93 pKa = 9.73LFRR96 pKa = 11.84LPLL99 pKa = 4.04

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5530

21

5551

2315541

13

5512

417.1

46.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.048 ± 0.026

1.411 ± 0.013

5.607 ± 0.022

6.296 ± 0.032

4.151 ± 0.02

5.62 ± 0.029

2.455 ± 0.015

6.325 ± 0.027

5.753 ± 0.027

9.695 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.012

4.161 ± 0.018

5.239 ± 0.033

4.123 ± 0.022

5.858 ± 0.025

8.53 ± 0.036

5.616 ± 0.02

5.887 ± 0.023

1.214 ± 0.013

2.899 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski