Lycianthes yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A140D6R5|A0A140D6R5_9GEMI Movement protein BC1 OS=Lycianthes yellow mosaic virus OX=1779714 GN=BC PE=3 SV=1
MM1 pKa = 7.33EE2 pKa = 5.69HH3 pKa = 6.59NNNAVAYY10 pKa = 5.35TTSDD14 pKa = 2.84RR15 pKa = 11.84TEE17 pKa = 3.9FQLSNEE23 pKa = 4.1KK24 pKa = 9.97TEE26 pKa = 4.14VTLLFPSTIDD36 pKa = 3.27QKK38 pKa = 11.06FSKK41 pKa = 10.79LLGKK45 pKa = 9.26CLRR48 pKa = 11.84IDD50 pKa = 3.67HH51 pKa = 6.78VILEE55 pKa = 4.31YY56 pKa = 10.8RR57 pKa = 11.84NQVPVNATGHH67 pKa = 5.14VVIEE71 pKa = 4.01MHH73 pKa = 6.5DD74 pKa = 3.69TRR76 pKa = 11.84LHH78 pKa = 6.18EE79 pKa = 4.92GDD81 pKa = 3.64SKK83 pKa = 10.99QAEE86 pKa = 4.06FTIPIGCNCNIHH98 pKa = 6.3YY99 pKa = 10.23YY100 pKa = 9.48SSSYY104 pKa = 10.47FSPKK108 pKa = 10.33DD109 pKa = 3.49PNPWRR114 pKa = 11.84VMYY117 pKa = 10.43RR118 pKa = 11.84VDD120 pKa = 3.44DD121 pKa = 4.19TNVVNGVHH129 pKa = 6.6FCRR132 pKa = 11.84MQGKK136 pKa = 9.91LKK138 pKa = 10.2MSSAKK143 pKa = 9.73QSSEE147 pKa = 3.7IQFKK151 pKa = 10.45SPKK154 pKa = 9.79IEE156 pKa = 4.1ILSKK160 pKa = 10.48AYY162 pKa = 11.2NMTNIDD168 pKa = 4.06FWTVPHH174 pKa = 6.11SQVSRR179 pKa = 11.84KK180 pKa = 8.79PVQALSNIRR189 pKa = 11.84SQSCRR194 pKa = 11.84YY195 pKa = 5.2TTPAILPGQTWASASSVGNEE215 pKa = 4.06DD216 pKa = 5.09QEE218 pKa = 4.34DD219 pKa = 3.71LPYY222 pKa = 11.02RR223 pKa = 11.84NLHH226 pKa = 5.9RR227 pKa = 11.84LQDD230 pKa = 3.65KK231 pKa = 8.97TLDD234 pKa = 4.11PGPSASEE241 pKa = 3.83VVGNNQTVNDD251 pKa = 4.22DD252 pKa = 3.87VINIIKK258 pKa = 8.83KK259 pKa = 7.36TVEE262 pKa = 3.62LCMEE266 pKa = 4.87GSNVTSNVKK275 pKa = 9.77PIKK278 pKa = 10.45

Molecular weight:
31.41 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A140D6R2|A0A140D6R2_9GEMI AC4 protein OS=Lycianthes yellow mosaic virus OX=1779714 GN=AC4 PE=3 SV=1
MM1 pKa = 7.38AVDD4 pKa = 4.58FRR6 pKa = 11.84TGEE9 pKa = 4.38YY10 pKa = 8.66ITATQAQNGAYY21 pKa = 8.83IWTVPNPLYY30 pKa = 10.81FKK32 pKa = 10.36VLKK35 pKa = 9.04HH36 pKa = 5.45TSRR39 pKa = 11.84PFNSDD44 pKa = 2.24HH45 pKa = 7.75DD46 pKa = 4.12YY47 pKa = 11.89LEE49 pKa = 4.04IQIQFNHH56 pKa = 5.87NLRR59 pKa = 11.84RR60 pKa = 11.84ALGIHH65 pKa = 5.86KK66 pKa = 9.88CFLIFRR72 pKa = 11.84IWTRR76 pKa = 11.84LRR78 pKa = 11.84PQTWRR83 pKa = 11.84FLRR86 pKa = 11.84VFKK89 pKa = 11.21YY90 pKa = 10.66NVIRR94 pKa = 11.84FLNNLGVVSINNVIRR109 pKa = 11.84SVNHH113 pKa = 5.24VLWNVFDD120 pKa = 3.6NTTYY124 pKa = 11.61ARR126 pKa = 11.84MLYY129 pKa = 10.06AIKK132 pKa = 10.51FNIYY136 pKa = 10.14

Molecular weight:
16.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1798

85

362

199.8

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.505 ± 0.528

2.336 ± 0.337

4.672 ± 0.36

4.171 ± 0.551

4.616 ± 0.508

5.061 ± 0.636

3.782 ± 0.392

6.34 ± 0.687

5.562 ± 0.513

7.341 ± 0.71

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.634

6.452 ± 0.6

5.006 ± 0.34

4.338 ± 0.543

6.952 ± 1.008

9.121 ± 0.79

5.228 ± 0.688

7.119 ± 0.788

1.168 ± 0.243

4.004 ± 0.617

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski