Amino acid dipepetide frequency for Gordonia phage Bizzy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.333AlaAla: 17.333 ± 1.371
0.818AlaCys: 0.818 ± 0.23
9.211AlaAsp: 9.211 ± 0.999
9.157AlaGlu: 9.157 ± 0.89
3.27AlaPhe: 3.27 ± 0.562
12.536AlaGly: 12.536 ± 1.221
2.017AlaHis: 2.017 ± 0.338
6.541AlaIle: 6.541 ± 1.002
3.979AlaLys: 3.979 ± 0.517
9.865AlaLeu: 9.865 ± 1.245
2.453AlaMet: 2.453 ± 0.386
3.052AlaAsn: 3.052 ± 0.623
6.323AlaPro: 6.323 ± 0.726
4.96AlaGln: 4.96 ± 0.455
8.285AlaArg: 8.285 ± 0.707
5.996AlaSer: 5.996 ± 0.777
5.996AlaThr: 5.996 ± 0.62
7.794AlaVal: 7.794 ± 0.565
2.344AlaTrp: 2.344 ± 0.318
2.344AlaTyr: 2.344 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
1.417CysAla: 1.417 ± 0.318
0.055CysCys: 0.055 ± 0.053
0.6CysAsp: 0.6 ± 0.205
0.491CysGlu: 0.491 ± 0.156
0.109CysPhe: 0.109 ± 0.072
1.635CysGly: 1.635 ± 0.488
0.273CysHis: 0.273 ± 0.141
0.109CysIle: 0.109 ± 0.098
0.109CysLys: 0.109 ± 0.07
0.436CysLeu: 0.436 ± 0.193
0.055CysMet: 0.055 ± 0.057
0.218CysAsn: 0.218 ± 0.112
0.545CysPro: 0.545 ± 0.18
0.327CysGln: 0.327 ± 0.171
0.382CysArg: 0.382 ± 0.167
0.709CysSer: 0.709 ± 0.222
0.382CysThr: 0.382 ± 0.142
0.382CysVal: 0.382 ± 0.13
0.055CysTrp: 0.055 ± 0.049
0.109CysTyr: 0.109 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
8.448AspAla: 8.448 ± 0.803
0.327AspCys: 0.327 ± 0.13
7.903AspAsp: 7.903 ± 1.166
5.832AspGlu: 5.832 ± 0.904
1.036AspPhe: 1.036 ± 0.27
7.849AspGly: 7.849 ± 0.803
1.744AspHis: 1.744 ± 0.33
1.744AspIle: 1.744 ± 0.255
1.199AspLys: 1.199 ± 0.241
5.232AspLeu: 5.232 ± 0.512
1.744AspMet: 1.744 ± 0.321
2.235AspAsn: 2.235 ± 0.332
7.249AspPro: 7.249 ± 0.827
2.562AspGln: 2.562 ± 0.478
5.014AspArg: 5.014 ± 0.634
2.507AspSer: 2.507 ± 0.273
3.597AspThr: 3.597 ± 0.485
5.778AspVal: 5.778 ± 0.501
1.581AspTrp: 1.581 ± 0.294
1.635AspTyr: 1.635 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
6.105GluAla: 6.105 ± 0.732
0.545GluCys: 0.545 ± 0.173
3.052GluAsp: 3.052 ± 0.41
1.254GluGlu: 1.254 ± 0.33
2.017GluPhe: 2.017 ± 0.319
3.652GluGly: 3.652 ± 0.553
2.017GluHis: 2.017 ± 0.403
2.507GluIle: 2.507 ± 0.241
1.417GluLys: 1.417 ± 0.319
5.232GluLeu: 5.232 ± 0.735
0.872GluMet: 0.872 ± 0.226
1.526GluAsn: 1.526 ± 0.257
3.216GluPro: 3.216 ± 0.496
3.488GluGln: 3.488 ± 0.552
3.761GluArg: 3.761 ± 0.535
2.344GluSer: 2.344 ± 0.42
3.379GluThr: 3.379 ± 0.498
4.633GluVal: 4.633 ± 0.541
1.69GluTrp: 1.69 ± 0.413
2.071GluTyr: 2.071 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
3.652PheAla: 3.652 ± 0.523
0.436PheCys: 0.436 ± 0.164
2.453PheAsp: 2.453 ± 0.346
1.69PheGlu: 1.69 ± 0.387
0.436PhePhe: 0.436 ± 0.207
3.052PheGly: 3.052 ± 0.395
0.709PheHis: 0.709 ± 0.189
1.199PheIle: 1.199 ± 0.23
0.6PheLys: 0.6 ± 0.165
1.69PheLeu: 1.69 ± 0.333
0.491PheMet: 0.491 ± 0.165
0.327PheAsn: 0.327 ± 0.168
0.872PhePro: 0.872 ± 0.239
0.382PheGln: 0.382 ± 0.137
1.199PheArg: 1.199 ± 0.24
1.036PheSer: 1.036 ± 0.215
1.635PheThr: 1.635 ± 0.236
1.526PheVal: 1.526 ± 0.246
0.273PheTrp: 0.273 ± 0.105
0.273PheTyr: 0.273 ± 0.119
0.0PheXaa: 0.0 ± 0.0
Gly
10.41GlyAla: 10.41 ± 1.283
0.872GlyCys: 0.872 ± 0.258
6.323GlyAsp: 6.323 ± 0.741
3.924GlyGlu: 3.924 ± 0.44
2.071GlyPhe: 2.071 ± 0.469
8.121GlyGly: 8.121 ± 1.339
2.671GlyHis: 2.671 ± 0.517
3.979GlyIle: 3.979 ± 0.522
2.398GlyLys: 2.398 ± 0.493
6.105GlyLeu: 6.105 ± 0.958
2.507GlyMet: 2.507 ± 0.323
2.18GlyAsn: 2.18 ± 0.376
4.142GlyPro: 4.142 ± 0.605
3.325GlyGln: 3.325 ± 0.495
7.522GlyArg: 7.522 ± 0.745
5.069GlySer: 5.069 ± 0.588
6.105GlyThr: 6.105 ± 0.764
7.195GlyVal: 7.195 ± 0.596
1.472GlyTrp: 1.472 ± 0.283
2.071GlyTyr: 2.071 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.799HisAla: 1.799 ± 0.294
0.055HisCys: 0.055 ± 0.055
1.581HisAsp: 1.581 ± 0.309
1.908HisGlu: 1.908 ± 0.364
0.273HisPhe: 0.273 ± 0.13
1.308HisGly: 1.308 ± 0.282
0.382HisHis: 0.382 ± 0.197
0.763HisIle: 0.763 ± 0.19
0.491HisLys: 0.491 ± 0.147
2.071HisLeu: 2.071 ± 0.372
0.981HisMet: 0.981 ± 0.253
0.273HisAsn: 0.273 ± 0.111
1.199HisPro: 1.199 ± 0.247
0.818HisGln: 0.818 ± 0.215
1.635HisArg: 1.635 ± 0.303
0.763HisSer: 0.763 ± 0.199
2.071HisThr: 2.071 ± 0.39
1.908HisVal: 1.908 ± 0.377
0.327HisTrp: 0.327 ± 0.153
0.327HisTyr: 0.327 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
5.996IleAla: 5.996 ± 0.635
0.164IleCys: 0.164 ± 0.093
3.761IleAsp: 3.761 ± 0.469
3.543IleGlu: 3.543 ± 0.446
0.436IlePhe: 0.436 ± 0.259
4.251IleGly: 4.251 ± 0.916
0.6IleHis: 0.6 ± 0.194
2.071IleIle: 2.071 ± 0.307
0.763IleLys: 0.763 ± 0.415
2.289IleLeu: 2.289 ± 0.308
0.654IleMet: 0.654 ± 0.187
0.709IleAsn: 0.709 ± 0.209
3.216IlePro: 3.216 ± 0.361
0.927IleGln: 0.927 ± 0.259
2.725IleArg: 2.725 ± 0.321
1.908IleSer: 1.908 ± 0.365
3.924IleThr: 3.924 ± 0.512
3.924IleVal: 3.924 ± 0.414
0.709IleTrp: 0.709 ± 0.19
0.709IleTyr: 0.709 ± 0.172
0.0IleXaa: 0.0 ± 0.0
Lys
2.943LysAla: 2.943 ± 0.454
0.055LysCys: 0.055 ± 0.05
1.09LysAsp: 1.09 ± 0.262
0.654LysGlu: 0.654 ± 0.18
1.036LysPhe: 1.036 ± 0.322
1.635LysGly: 1.635 ± 0.345
0.382LysHis: 0.382 ± 0.115
1.69LysIle: 1.69 ± 0.357
0.654LysLys: 0.654 ± 0.169
1.581LysLeu: 1.581 ± 0.222
0.382LysMet: 0.382 ± 0.123
0.6LysAsn: 0.6 ± 0.199
1.363LysPro: 1.363 ± 0.277
1.036LysGln: 1.036 ± 0.254
2.289LysArg: 2.289 ± 0.386
1.635LysSer: 1.635 ± 0.359
1.526LysThr: 1.526 ± 0.226
1.853LysVal: 1.853 ± 0.294
0.545LysTrp: 0.545 ± 0.163
0.763LysTyr: 0.763 ± 0.199
0.0LysXaa: 0.0 ± 0.0
Leu
9.484LeuAla: 9.484 ± 0.929
0.872LeuCys: 0.872 ± 0.211
5.723LeuAsp: 5.723 ± 0.729
2.562LeuGlu: 2.562 ± 0.431
2.126LeuPhe: 2.126 ± 0.392
7.14LeuGly: 7.14 ± 1.323
1.581LeuHis: 1.581 ± 0.336
2.834LeuIle: 2.834 ± 0.486
1.036LeuLys: 1.036 ± 0.278
5.505LeuLeu: 5.505 ± 0.635
1.308LeuMet: 1.308 ± 0.21
2.017LeuAsn: 2.017 ± 0.304
5.178LeuPro: 5.178 ± 0.489
3.27LeuGln: 3.27 ± 0.469
6.214LeuArg: 6.214 ± 0.415
3.107LeuSer: 3.107 ± 0.341
5.396LeuThr: 5.396 ± 0.506
6.977LeuVal: 6.977 ± 0.724
1.635LeuTrp: 1.635 ± 0.305
1.472LeuTyr: 1.472 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
1.635MetAla: 1.635 ± 0.314
0.164MetCys: 0.164 ± 0.089
1.09MetAsp: 1.09 ± 0.216
0.709MetGlu: 0.709 ± 0.25
0.491MetPhe: 0.491 ± 0.181
1.308MetGly: 1.308 ± 0.247
0.436MetHis: 0.436 ± 0.146
0.872MetIle: 0.872 ± 0.17
0.654MetLys: 0.654 ± 0.166
2.344MetLeu: 2.344 ± 0.369
0.327MetMet: 0.327 ± 0.129
0.763MetAsn: 0.763 ± 0.187
1.853MetPro: 1.853 ± 0.313
1.036MetGln: 1.036 ± 0.379
1.581MetArg: 1.581 ± 0.275
1.363MetSer: 1.363 ± 0.245
3.161MetThr: 3.161 ± 0.397
1.69MetVal: 1.69 ± 0.25
0.327MetTrp: 0.327 ± 0.118
0.218MetTyr: 0.218 ± 0.106
0.0MetXaa: 0.0 ± 0.0
Asn
3.488AsnAla: 3.488 ± 0.483
0.382AsnCys: 0.382 ± 0.122
2.398AsnAsp: 2.398 ± 0.316
1.036AsnGlu: 1.036 ± 0.242
0.545AsnPhe: 0.545 ± 0.146
2.998AsnGly: 2.998 ± 0.43
0.436AsnHis: 0.436 ± 0.158
1.036AsnIle: 1.036 ± 0.281
0.545AsnLys: 0.545 ± 0.179
1.962AsnLeu: 1.962 ± 0.321
0.709AsnMet: 0.709 ± 0.214
0.818AsnAsn: 0.818 ± 0.19
2.398AsnPro: 2.398 ± 0.327
0.491AsnGln: 0.491 ± 0.177
1.363AsnArg: 1.363 ± 0.318
1.363AsnSer: 1.363 ± 0.288
1.308AsnThr: 1.308 ± 0.221
2.126AsnVal: 2.126 ± 0.324
0.709AsnTrp: 0.709 ± 0.204
0.545AsnTyr: 0.545 ± 0.172
0.0AsnXaa: 0.0 ± 0.0
Pro
8.448ProAla: 8.448 ± 0.913
0.273ProCys: 0.273 ± 0.135
5.996ProAsp: 5.996 ± 0.868
3.979ProGlu: 3.979 ± 0.72
1.635ProPhe: 1.635 ± 0.342
5.232ProGly: 5.232 ± 0.525
1.199ProHis: 1.199 ± 0.285
3.543ProIle: 3.543 ± 0.419
1.417ProLys: 1.417 ± 0.3
4.469ProLeu: 4.469 ± 0.551
1.363ProMet: 1.363 ± 0.261
2.126ProAsn: 2.126 ± 0.331
4.36ProPro: 4.36 ± 0.699
1.635ProGln: 1.635 ± 0.276
3.488ProArg: 3.488 ± 0.499
2.834ProSer: 2.834 ± 0.358
4.251ProThr: 4.251 ± 0.534
5.232ProVal: 5.232 ± 0.71
0.927ProTrp: 0.927 ± 0.233
1.363ProTyr: 1.363 ± 0.257
0.0ProXaa: 0.0 ± 0.0
Gln
5.287GlnAla: 5.287 ± 0.518
0.382GlnCys: 0.382 ± 0.159
0.927GlnAsp: 0.927 ± 0.277
1.09GlnGlu: 1.09 ± 0.248
1.09GlnPhe: 1.09 ± 0.247
1.853GlnGly: 1.853 ± 0.32
0.763GlnHis: 0.763 ± 0.222
1.526GlnIle: 1.526 ± 0.291
0.818GlnLys: 0.818 ± 0.212
2.998GlnLeu: 2.998 ± 0.412
0.981GlnMet: 0.981 ± 0.212
0.927GlnAsn: 0.927 ± 0.217
3.052GlnPro: 3.052 ± 0.445
2.834GlnGln: 2.834 ± 0.411
3.815GlnArg: 3.815 ± 0.592
1.799GlnSer: 1.799 ± 0.373
2.507GlnThr: 2.507 ± 0.386
3.27GlnVal: 3.27 ± 0.447
0.981GlnTrp: 0.981 ± 0.2
0.818GlnTyr: 0.818 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
9.266ArgAla: 9.266 ± 0.818
0.654ArgCys: 0.654 ± 0.187
4.742ArgAsp: 4.742 ± 0.51
3.325ArgGlu: 3.325 ± 0.393
1.69ArgPhe: 1.69 ± 0.333
5.014ArgGly: 5.014 ± 0.744
1.199ArgHis: 1.199 ± 0.305
3.761ArgIle: 3.761 ± 0.57
2.235ArgLys: 2.235 ± 0.427
5.069ArgLeu: 5.069 ± 0.558
2.344ArgMet: 2.344 ± 0.318
2.507ArgAsn: 2.507 ± 0.351
3.652ArgPro: 3.652 ± 0.56
2.998ArgGln: 2.998 ± 0.415
9.211ArgArg: 9.211 ± 1.207
3.161ArgSer: 3.161 ± 0.422
3.924ArgThr: 3.924 ± 0.507
5.887ArgVal: 5.887 ± 0.649
1.853ArgTrp: 1.853 ± 0.402
2.18ArgTyr: 2.18 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
5.669SerAla: 5.669 ± 0.76
0.436SerCys: 0.436 ± 0.151
2.889SerAsp: 2.889 ± 0.485
2.507SerGlu: 2.507 ± 0.447
1.09SerPhe: 1.09 ± 0.259
5.287SerGly: 5.287 ± 0.778
1.09SerHis: 1.09 ± 0.195
2.126SerIle: 2.126 ± 0.272
1.199SerLys: 1.199 ± 0.34
3.434SerLeu: 3.434 ± 0.476
0.927SerMet: 0.927 ± 0.242
1.036SerAsn: 1.036 ± 0.241
2.998SerPro: 2.998 ± 0.424
1.581SerGln: 1.581 ± 0.378
3.161SerArg: 3.161 ± 0.428
3.434SerSer: 3.434 ± 0.665
3.761SerThr: 3.761 ± 0.529
3.815SerVal: 3.815 ± 0.418
1.363SerTrp: 1.363 ± 0.349
0.981SerTyr: 0.981 ± 0.248
0.0SerXaa: 0.0 ± 0.0
Thr
8.612ThrAla: 8.612 ± 0.761
0.654ThrCys: 0.654 ± 0.194
5.123ThrAsp: 5.123 ± 0.558
3.706ThrGlu: 3.706 ± 0.462
1.635ThrPhe: 1.635 ± 0.34
5.396ThrGly: 5.396 ± 0.518
0.981ThrHis: 0.981 ± 0.289
2.725ThrIle: 2.725 ± 0.38
1.744ThrLys: 1.744 ± 0.294
5.287ThrLeu: 5.287 ± 0.46
1.363ThrMet: 1.363 ± 0.214
2.126ThrAsn: 2.126 ± 0.339
5.396ThrPro: 5.396 ± 0.689
1.526ThrGln: 1.526 ± 0.239
3.924ThrArg: 3.924 ± 0.535
3.597ThrSer: 3.597 ± 0.431
4.251ThrThr: 4.251 ± 0.566
5.341ThrVal: 5.341 ± 0.576
1.254ThrTrp: 1.254 ± 0.218
1.145ThrTyr: 1.145 ± 0.232
0.0ThrXaa: 0.0 ± 0.0
Val
9.266ValAla: 9.266 ± 0.697
0.927ValCys: 0.927 ± 0.341
7.685ValAsp: 7.685 ± 0.758
4.796ValGlu: 4.796 ± 0.52
1.581ValPhe: 1.581 ± 0.26
7.304ValGly: 7.304 ± 0.669
1.472ValHis: 1.472 ± 0.279
2.889ValIle: 2.889 ± 0.458
1.635ValLys: 1.635 ± 0.239
5.287ValLeu: 5.287 ± 0.561
1.199ValMet: 1.199 ± 0.206
2.289ValAsn: 2.289 ± 0.428
4.306ValPro: 4.306 ± 0.46
2.943ValGln: 2.943 ± 0.312
5.396ValArg: 5.396 ± 0.518
3.815ValSer: 3.815 ± 0.451
5.996ValThr: 5.996 ± 0.601
6.868ValVal: 6.868 ± 0.69
2.126ValTrp: 2.126 ± 0.276
1.799ValTyr: 1.799 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
2.725TrpAla: 2.725 ± 0.39
0.273TrpCys: 0.273 ± 0.116
1.145TrpAsp: 1.145 ± 0.233
0.981TrpGlu: 0.981 ± 0.244
0.654TrpPhe: 0.654 ± 0.179
0.872TrpGly: 0.872 ± 0.228
0.491TrpHis: 0.491 ± 0.165
0.981TrpIle: 0.981 ± 0.266
0.6TrpLys: 0.6 ± 0.18
2.562TrpLeu: 2.562 ± 0.387
0.763TrpMet: 0.763 ± 0.175
0.491TrpAsn: 0.491 ± 0.226
1.254TrpPro: 1.254 ± 0.232
0.927TrpGln: 0.927 ± 0.19
1.799TrpArg: 1.799 ± 0.33
1.363TrpSer: 1.363 ± 0.273
1.036TrpThr: 1.036 ± 0.224
1.308TrpVal: 1.308 ± 0.268
0.436TrpTrp: 0.436 ± 0.17
0.382TrpTyr: 0.382 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.289TyrAla: 2.289 ± 0.433
0.055TyrCys: 0.055 ± 0.06
1.526TyrAsp: 1.526 ± 0.342
1.635TyrGlu: 1.635 ± 0.313
0.709TyrPhe: 0.709 ± 0.164
2.017TyrGly: 2.017 ± 0.272
0.491TyrHis: 0.491 ± 0.18
0.382TyrIle: 0.382 ± 0.154
0.273TyrLys: 0.273 ± 0.101
2.017TyrLeu: 2.017 ± 0.371
0.545TyrMet: 0.545 ± 0.152
0.436TyrAsn: 0.436 ± 0.174
1.199TyrPro: 1.199 ± 0.243
0.654TyrGln: 0.654 ± 0.174
1.908TyrArg: 1.908 ± 0.387
0.981TyrSer: 0.981 ± 0.192
1.581TyrThr: 1.581 ± 0.343
2.071TyrVal: 2.071 ± 0.405
0.436TyrTrp: 0.436 ± 0.147
0.382TyrTyr: 0.382 ± 0.113
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (18348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski